FI104496B - Yliekspressiojärjestelmät kolera B-alayksikön ekspressiota varten vieraiden promoottoreiden ja johtopeptidien avulla - Google Patents
Yliekspressiojärjestelmät kolera B-alayksikön ekspressiota varten vieraiden promoottoreiden ja johtopeptidien avulla Download PDFInfo
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- FI104496B FI104496B FI894368A FI894368A FI104496B FI 104496 B FI104496 B FI 104496B FI 894368 A FI894368 A FI 894368A FI 894368 A FI894368 A FI 894368A FI 104496 B FI104496 B FI 104496B
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- 230000003993 interaction Effects 0.000 description 1
- 230000008944 intestinal immunity Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000010253 intravenous injection Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000007169 ligase reaction Methods 0.000 description 1
- ADKOXSOCTOWDOP-UHFFFAOYSA-L magnesium;aluminum;dihydroxide;trihydrate Chemical compound O.O.O.[OH-].[OH-].[Mg+2].[Al] ADKOXSOCTOWDOP-UHFFFAOYSA-L 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 238000006384 oligomerization reaction Methods 0.000 description 1
- 229940126578 oral vaccine Drugs 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 102000013415 peroxidase activity proteins Human genes 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000024 polymyxin B Polymers 0.000 description 1
- 229960005266 polymyxin b Drugs 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011027 product recovery Methods 0.000 description 1
- 238000011321 prophylaxis Methods 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 210000005239 tubule Anatomy 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 230000009452 underexpressoin Effects 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/28—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Vibrionaceae (F)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Soy Sauces And Products Related Thereto (AREA)
- Compositions Of Macromolecular Compounds (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
- Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
- Measuring And Recording Apparatus For Diagnosis (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
- Exhaust Gas Treatment By Means Of Catalyst (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE8803291A SE462285B (sv) | 1988-09-16 | 1988-09-16 | Expression av koleratoxinets bindande subenhet med hjaelp av fraemmande promotor- och/eller ledarpeptidstrukturer |
SE8803291 | 1988-09-16 |
Publications (3)
Publication Number | Publication Date |
---|---|
FI894368A0 FI894368A0 (fi) | 1989-09-15 |
FI894368A FI894368A (fi) | 1990-03-17 |
FI104496B true FI104496B (fi) | 2000-02-15 |
Family
ID=20373364
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FI894368A FI104496B (fi) | 1988-09-16 | 1989-09-15 | Yliekspressiojärjestelmät kolera B-alayksikön ekspressiota varten vieraiden promoottoreiden ja johtopeptidien avulla |
Country Status (13)
Country | Link |
---|---|
EP (1) | EP0368819B1 (ja) |
JP (1) | JP2790333B2 (ja) |
AT (1) | ATE161885T1 (ja) |
AU (1) | AU633842B2 (ja) |
CA (1) | CA1340577C (ja) |
DE (1) | DE68928529T2 (ja) |
DK (1) | DK175694B1 (ja) |
ES (1) | ES2113342T3 (ja) |
FI (1) | FI104496B (ja) |
GR (1) | GR3025846T3 (ja) |
IS (1) | IS1804B (ja) |
NO (1) | NO301175B1 (ja) |
SE (1) | SE462285B (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109096403A (zh) * | 2018-07-04 | 2018-12-28 | 浙江工商大学 | 一种用于蛋白质转导的蛋白质载体及其制备方法和应用 |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0416505A3 (en) * | 1989-09-04 | 1991-10-02 | Takeda Chemical Industries, Ltd. | Expression plasmids and use thereof |
US5356797A (en) * | 1991-11-15 | 1994-10-18 | Board Of Regents, The University Of Texas | Membrane expression of heterologous genes |
EP0613500A1 (en) * | 1991-11-15 | 1994-09-07 | The Board Of Regents, The University Of Texas System | Membrane expression of heterologous genes |
US5932714A (en) * | 1995-02-23 | 1999-08-03 | Connaught Laboratories Limited | Expression of gene products from genetically manipulated strains of Bordetella |
US5998168A (en) * | 1995-06-07 | 1999-12-07 | Connaught Laboratories Limited | Expression of gene products from genetically manipulated strains of bordetella |
US6008329A (en) * | 1998-03-06 | 1999-12-28 | The United States Of America As Represented By The Secretary Of The Army | Method for purifying cholera toxin |
WO2007117339A2 (en) * | 2006-01-11 | 2007-10-18 | The United States Of America As Respresented By The Secretary Of The Navy | Adhesin-enterotoxin chimera based immunogenic composition against entertoxigenic escherichia coli |
DE102011111688A1 (de) * | 2011-08-24 | 2013-02-28 | Bioserv Analytik Und Medizinprodukte Gmbh | Verfahren zur schnellen Isolation bzw. Aufreinigung von rekombinanten Proteinen mit einer (nicht toxischen) Choleratoxin-Domäne |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4666837A (en) * | 1982-05-24 | 1987-05-19 | Smithkline-Rit | DNA sequences, recombinant DNA molecules and processes for producing the A and B subunits of cholera toxin and preparations containing so-obtained subunit or subunits |
US5135862A (en) * | 1983-03-04 | 1992-08-04 | University Of Maryland | Method of isolating restriction fragment deletions in vibrio cholerae, products thereof |
US4882278A (en) * | 1983-04-29 | 1989-11-21 | President And Fellows Of Harvard College | Non-toxinogenic vibrio cholerae mutants |
EP0251579A3 (en) * | 1986-06-24 | 1989-03-22 | Enterovax Research Pty. Ltd. | Non-antibiotic marker system |
HU205372B (en) * | 1987-04-29 | 1992-04-28 | Harvard College | Process for producing vaccines against cholera |
-
1988
- 1988-09-16 SE SE8803291A patent/SE462285B/sv not_active IP Right Cessation
-
1989
- 1989-09-04 IS IS3505A patent/IS1804B/is unknown
- 1989-09-11 EP EP89850295A patent/EP0368819B1/en not_active Expired - Lifetime
- 1989-09-11 DE DE68928529T patent/DE68928529T2/de not_active Expired - Lifetime
- 1989-09-11 ES ES89850295T patent/ES2113342T3/es not_active Expired - Lifetime
- 1989-09-11 AT AT89850295T patent/ATE161885T1/de not_active IP Right Cessation
- 1989-09-14 JP JP1239733A patent/JP2790333B2/ja not_active Expired - Lifetime
- 1989-09-15 DK DK198904569A patent/DK175694B1/da not_active IP Right Cessation
- 1989-09-15 NO NO893702A patent/NO301175B1/no not_active IP Right Cessation
- 1989-09-15 FI FI894368A patent/FI104496B/fi active IP Right Grant
- 1989-09-15 AU AU41349/89A patent/AU633842B2/en not_active Expired
- 1989-09-15 CA CA000611596A patent/CA1340577C/en not_active Expired - Fee Related
-
1998
- 1998-01-08 GR GR970402939T patent/GR3025846T3/el unknown
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109096403A (zh) * | 2018-07-04 | 2018-12-28 | 浙江工商大学 | 一种用于蛋白质转导的蛋白质载体及其制备方法和应用 |
CN109096403B (zh) * | 2018-07-04 | 2020-06-30 | 浙江工商大学 | 一种用于蛋白质转导的蛋白质载体及其制备方法和应用 |
Also Published As
Publication number | Publication date |
---|---|
ES2113342T3 (es) | 1998-05-01 |
NO893702L (no) | 1990-03-19 |
ATE161885T1 (de) | 1998-01-15 |
SE462285B (sv) | 1990-05-28 |
GR3025846T3 (en) | 1998-04-30 |
AU633842B2 (en) | 1993-02-11 |
IS1804B (is) | 2002-02-26 |
FI894368A (fi) | 1990-03-17 |
JPH0322997A (ja) | 1991-01-31 |
DK456989A (da) | 1990-03-17 |
FI894368A0 (fi) | 1989-09-15 |
DE68928529D1 (de) | 1998-02-12 |
CA1340577C (en) | 1999-06-01 |
DK175694B1 (da) | 2005-01-24 |
DE68928529T2 (de) | 1998-04-16 |
IS3505A7 (is) | 1990-03-17 |
JP2790333B2 (ja) | 1998-08-27 |
NO301175B1 (no) | 1997-09-22 |
AU4134989A (en) | 1990-04-05 |
SE8803291D0 (sv) | 1988-09-16 |
NO893702D0 (no) | 1989-09-15 |
DK456989D0 (da) | 1989-09-15 |
EP0368819B1 (en) | 1998-01-07 |
EP0368819A1 (en) | 1990-05-16 |
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Legal Events
Date | Code | Title | Description |
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GB | Transfer or assigment of application |
Owner name: VITEC AKTIEBOLAG |
|
FG | Patent granted |
Owner name: VITEC AKTIEBOLAG |