ES8802162A1 - Un procedimiento para la preparacion de una levadura trans- formada con un plasmido recombinante insertado con el gen del virus de la hepatitis b. - Google Patents

Un procedimiento para la preparacion de una levadura trans- formada con un plasmido recombinante insertado con el gen del virus de la hepatitis b.

Info

Publication number
ES8802162A1
ES8802162A1 ES540895A ES540895A ES8802162A1 ES 8802162 A1 ES8802162 A1 ES 8802162A1 ES 540895 A ES540895 A ES 540895A ES 540895 A ES540895 A ES 540895A ES 8802162 A1 ES8802162 A1 ES 8802162A1
Authority
ES
Spain
Prior art keywords
hepatitis
virus
recombinant plasmid
gene
yeast
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES540895A
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English (en)
Other versions
ES540895A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institute of Advanced Industrial Science and Technology AIST
Original Assignee
Agency of Industrial Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency of Industrial Science and Technology filed Critical Agency of Industrial Science and Technology
Publication of ES8802162A1 publication Critical patent/ES8802162A1/es
Publication of ES540895A0 publication Critical patent/ES540895A0/es
Expired legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/40Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00Reverse transcribing DNA viruses
    • C12N2730/00011Details
    • C12N2730/10011Hepadnaviridae
    • C12N2730/10111Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Saccharide Compounds (AREA)

Abstract

LA PREPARACION DE UNA LEVADURA TRANSFORMADA CON UN PLASMIDO RECOMBINANTE, INSERTADO CON EL GEN DEL VIRUS DE LA HEPATITIS B; SE LLEVA A CABO POR: A) RECOMBINACION DE UN GEN ANTIGENO HBS Y EN UN VECTOR REVERSIBLE QUE CONTIENE UN GEN DE LEVADURA Y UN GEN DE ESCHERICHIA COLI, EL CUAL CONSTA DE UNA SECUENCIA DE ADN QUE ES NECESARIA PARA LA REPLICACION DEL GEN EN CELULAS DE E.COLI Y QUE LLEVA UNA REGION DE CONTROL DE LA EXPRESION DEL GEN DE FOSFATASA ACIDA REPRIMIBLE (POLIPEPTIDO DE 60.000 DALTON); Y B) TRANSFORMACION DE UNA LEVADURA, QUE ES UNA CEPA MUTANTE QUE REQUIERE HISTIDINA, TRIPTOFANO, URACILO, ADENINA O PREFERIBLEMENTE LEUCINA, TIPO SACCHAROMYCES CEREVISIAE AH22 CA, LEN2, HIS4, CAU1 (CIRB), CON EL PLASMIDO RECOMBINANTE DE A), MEDIANTE LA MEZCLA DEL DNA DEL PLASMIDO CON CELULAS DE LEVADURAS PROCEDENTES DE SU TRANSFORMACION EN ESFEROPLASTOS, SEGUIDO DE TRATAMIENTO CON CALCIO.
ES540895A 1982-08-16 1985-03-01 Un procedimiento para la preparacion de una levadura trans- formada con un plasmido recombinante insertado con el gen del virus de la hepatitis b. Expired ES8802162A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP57142460A JPS5931799A (ja) 1982-08-16 1982-08-16 B型肝炎ウイルス遺伝子を組込んだ組換えプラスミド

Publications (2)

Publication Number Publication Date
ES8802162A1 true ES8802162A1 (es) 1988-04-01
ES540895A0 ES540895A0 (es) 1988-04-01

Family

ID=15315829

Family Applications (2)

Application Number Title Priority Date Filing Date
ES83524942A Expired ES8600059A1 (es) 1982-08-16 1983-08-12 Procedimiento para la preparacion de un plasmido recombinante insertado con el gen del virus de la hepatitis b.
ES540895A Expired ES8802162A1 (es) 1982-08-16 1985-03-01 Un procedimiento para la preparacion de una levadura trans- formada con un plasmido recombinante insertado con el gen del virus de la hepatitis b.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES83524942A Expired ES8600059A1 (es) 1982-08-16 1983-08-12 Procedimiento para la preparacion de un plasmido recombinante insertado con el gen del virus de la hepatitis b.

Country Status (8)

Country Link
US (1) US4778761A (es)
JP (1) JPS5931799A (es)
KR (1) KR890000096B1 (es)
CA (1) CA1270217A (es)
DK (1) DK169953B1 (es)
ES (2) ES8600059A1 (es)
IL (1) IL69423A0 (es)
SU (1) SU1387878A3 (es)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2125047B (en) * 1982-08-09 1986-02-19 Ciba Geigy Ag Yeast hybrid vectors and their use for the production of polypeptides
JPS5936699A (ja) * 1982-08-20 1984-02-28 Chemo Sero Therapeut Res Inst シヤトルベクタ−
JPS5974985A (ja) * 1982-10-19 1984-04-27 Takeda Chem Ind Ltd 新規dna
JPS61139391A (ja) * 1984-12-11 1986-06-26 Chemo Sero Therapeut Res Inst 単純ヘルペスウィルス遺伝子を組込んだ組換えプラスミドおよび形質転換酵母
US4816564A (en) * 1986-01-31 1989-03-28 Merck & Co., Inc. Method for producing hepatitis B virus proteins in yeast
US5190877A (en) * 1987-09-03 1993-03-02 Gist-Brocades N.V. Saccharomyces strains for maltose fermentation
US4883865A (en) * 1987-09-30 1989-11-28 Merck & Co. Inc. Recovery of pres2+s antigen
AP129A (en) * 1988-06-03 1991-04-17 Smithkline Biologicals S A Expression of retrovirus gag protein eukaryotic cells
US6319501B1 (en) * 1990-07-19 2001-11-20 Health Research, Inc. Method of immunizing against hepatitis B virus
LT3988B (en) 1992-02-17 1996-06-25 Fermentas Biotech Inst Recombinant plasmides pfs19, pfps2-48 and pjlfds1 codingsynthesis of human hepatite b of surfice virus antigenes, methods fof producing thereof
US5531990A (en) * 1993-12-15 1996-07-02 Health Research, Inc. Method of raising an immune response with an anti-idiotypic antibody having correspondence with human hepatitis B surface antigen
IL118626A0 (en) * 1996-06-11 1996-10-16 Xtl Biopharmaceuticals Limited Anti HBV antibody
US6451572B1 (en) 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
ES2248067T3 (es) 1999-03-31 2006-03-16 Cornell Research Foundation, Inc. Fosfatasas con actividad de fitasa mejorada.
US6841370B1 (en) * 1999-11-18 2005-01-11 Cornell Research Foundation, Inc. Site-directed mutagenesis of Escherichia coli phytase
AU2002356880A1 (en) 2001-10-31 2003-05-12 Phytex, Llc Phytase-containing animal food and method
CA2498017C (en) * 2002-09-13 2016-06-14 Cornell Research Foundation, Inc. Using mutations to improve aspergillus phytases
US7919297B2 (en) 2006-02-21 2011-04-05 Cornell Research Foundation, Inc. Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase
WO2008017066A2 (en) * 2006-08-03 2008-02-07 Cornell Research Foundation, Inc. Phytases with improved thermal stability
SI2147105T1 (sl) 2007-05-02 2013-08-30 Merial Limited DNA-plazmidi z izboljšano ekspresijo in stabilnostjo
US8192734B2 (en) 2007-07-09 2012-06-05 Cornell University Compositions and methods for bone strengthening
CN112680464B (zh) * 2020-12-10 2022-04-26 南京农业大学 一种单甲基砷和三价锑氧化酶基因arsV及其编码的蛋白和应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2480779B2 (fr) * 1979-08-30 1986-07-18 Anvar Vecteur contenant une sequence nucleotidique de l'antigene de surface du virus de l'hepatite b et procede de fabrication d'une molecule immunogene mettant en oeuvre ce vecteur
FR2444713A1 (fr) * 1978-12-18 1980-07-18 Pasteur Institut Procede de production d'un adn comprenant le genome du virus de l'hepatite b et vecteur le comportant
DK368882A (da) * 1981-08-25 1983-02-26 Alan John Kingsman Expessions vektorer
US4446235A (en) * 1982-03-22 1984-05-01 Genentech, Inc. Method for cloning human growth hormone varient genes
US4546082A (en) * 1982-06-17 1985-10-08 Regents Of The Univ. Of California E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins
GB2125047B (en) * 1982-08-09 1986-02-19 Ciba Geigy Ag Yeast hybrid vectors and their use for the production of polypeptides
IL69202A (en) * 1982-09-08 1991-08-16 Smith Kline Rit Surface antigen polypeptides of hbv virus produced in yeast and the preparation thereof
CA1341116C (en) * 1983-02-22 2000-10-17 Rae Lyn Burke Yeast expression systems with vectors having gapdh or pyk promoters and synthesis or foreign protein

Also Published As

Publication number Publication date
US4778761A (en) 1988-10-18
DK169953B1 (da) 1995-04-10
IL69423A0 (en) 1983-11-30
DK372183D0 (da) 1983-08-15
ES540895A0 (es) 1988-04-01
KR890000096B1 (ko) 1989-03-07
JPS5931799A (ja) 1984-02-20
CA1270217A (en) 1990-06-12
KR840005745A (ko) 1984-11-15
ES524942A0 (es) 1985-10-01
DK372183A (da) 1984-02-17
ES8600059A1 (es) 1985-10-01
SU1387878A3 (ru) 1988-04-07
JPS6155950B2 (es) 1986-11-29

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Effective date: 20151230