ES8802075A1 - Procedimiento para la multiplicacion de un adn heterologo - Google Patents
Procedimiento para la multiplicacion de un adn heterologoInfo
- Publication number
- ES8802075A1 ES8802075A1 ES556975A ES556975A ES8802075A1 ES 8802075 A1 ES8802075 A1 ES 8802075A1 ES 556975 A ES556975 A ES 556975A ES 556975 A ES556975 A ES 556975A ES 8802075 A1 ES8802075 A1 ES 8802075A1
- Authority
- ES
- Spain
- Prior art keywords
- preparation
- yeast vector
- multicopy yeast
- vectors
- multicopy
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/68—Stabilisation of the vector
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Metal Extraction Processes (AREA)
- Manufacturing Cores, Coils, And Magnets (AREA)
- Production Of Multi-Layered Print Wiring Board (AREA)
Abstract
PROCEDIMIENTO PARA LA MULTIPLICACION DE UN ADN HETEROLOGO. COMPRENDE LAS OPERACIONES DE INSERTAR UN ADN HETEROLOGO, QUE CODIFICA UNA PROTEINA DESEADA, EN UN VECTOR DE LEVADURA DE COPIAS MULTIPLES, ESTABLE Y RECOMBINANTE; DE TRANSFORMAR EL VECTOR DENTRO DE UN ORGANISMO HOSPEDANTE DE LEVADURA APROPIADO QUE CONTIENE COPIAS DE <TIPO SILVESTRE> DE PLASMIDO CIRCULO 2/U; DE CULTIVAR EL HOSPEDANTE PRIMERAMENTE SOBRE UN MEDIOQUE DESACTIVA AL PROMOTOR REGULABLE; DE CULTIVAR EL HOSPEDANTE SOBRE UN MEDIO QUE REACTIVA AL PROMOTOR REGULABLE; DE TRANSFERIR EL HOSPEDANTE DE NUEVO AL MEDIO DESACTIVADOR DEL PROMOTOR REGULABLE; DE AISLAR Y PURIFICAR LOS VECTORES PROCEDENTES DE LAS CELULAS; Y DE AISLAR EL ADN HETEROLOGO A PARTIR DE LOS VECTORES Y DEPURIFICARLO A CONTINUACION.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19843444495 DE3444495A1 (de) | 1984-12-04 | 1984-12-04 | Herstellung eines multi-kopien-hefe-vektors |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES556975A0 ES556975A0 (es) | 1988-03-16 |
| ES8802075A1 true ES8802075A1 (es) | 1988-03-16 |
Family
ID=6252051
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES549526A Expired ES8800342A1 (es) | 1984-12-04 | 1985-12-03 | Procedimiento para preparar un vector de levadura de copias multiples,estables y recombinante |
| ES556975A Expired ES8802075A1 (es) | 1984-12-04 | 1986-07-23 | Procedimiento para la multiplicacion de un adn heterologo |
| ES556976A Expired ES8801705A1 (es) | 1984-12-04 | 1986-07-23 | Procedimiento para la expresion de una proteina heterologa |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES549526A Expired ES8800342A1 (es) | 1984-12-04 | 1985-12-03 | Procedimiento para preparar un vector de levadura de copias multiples,estables y recombinante |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES556976A Expired ES8801705A1 (es) | 1984-12-04 | 1986-07-23 | Procedimiento para la expresion de una proteina heterologa |
Country Status (15)
| Country | Link |
|---|---|
| EP (1) | EP0184163A3 (es) |
| JP (1) | JPS61181385A (es) |
| AU (1) | AU5070685A (es) |
| DE (1) | DE3444495A1 (es) |
| DK (1) | DK559585A (es) |
| ES (3) | ES8800342A1 (es) |
| FI (1) | FI854781A (es) |
| GR (1) | GR852891B (es) |
| HU (1) | HU196454B (es) |
| NO (1) | NO854864L (es) |
| NZ (1) | NZ214422A (es) |
| PT (1) | PT81594B (es) |
| SU (1) | SU1364241A3 (es) |
| YU (1) | YU186985A (es) |
| ZA (1) | ZA859240B (es) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3605035A1 (de) * | 1986-02-18 | 1987-08-20 | Strahlen Umweltforsch Gmbh | Plasmid pws101, verfahren zu seiner gewinnung und seine verwendung |
| EP3228707B1 (en) * | 2014-12-03 | 2020-09-23 | Kaneka Corporation | Vector containing centromere dna sequence and use thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL63035A (en) * | 1980-09-09 | 1985-05-31 | Univ California | Dnas capable of replication and stable mitotic maintenance in a host eukaryote,their preparation and eukaryotic cells comprising them |
-
1984
- 1984-12-04 DE DE19843444495 patent/DE3444495A1/de not_active Withdrawn
-
1985
- 1985-11-30 EP EP85115203A patent/EP0184163A3/de not_active Withdrawn
- 1985-12-02 YU YU01869/85A patent/YU186985A/xx unknown
- 1985-12-02 GR GR852891A patent/GR852891B/el unknown
- 1985-12-03 DK DK559585A patent/DK559585A/da not_active Application Discontinuation
- 1985-12-03 ZA ZA859240A patent/ZA859240B/xx unknown
- 1985-12-03 NO NO854864A patent/NO854864L/no unknown
- 1985-12-03 PT PT81594A patent/PT81594B/pt unknown
- 1985-12-03 HU HU854619A patent/HU196454B/hu not_active IP Right Cessation
- 1985-12-03 ES ES549526A patent/ES8800342A1/es not_active Expired
- 1985-12-03 SU SU853991833A patent/SU1364241A3/ru active
- 1985-12-03 AU AU50706/85A patent/AU5070685A/en not_active Abandoned
- 1985-12-03 FI FI854781A patent/FI854781A/fi not_active IP Right Cessation
- 1985-12-03 NZ NZ214422A patent/NZ214422A/en unknown
- 1985-12-04 JP JP60273181A patent/JPS61181385A/ja active Pending
-
1986
- 1986-07-23 ES ES556975A patent/ES8802075A1/es not_active Expired
- 1986-07-23 ES ES556976A patent/ES8801705A1/es not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| NO854864L (no) | 1986-06-05 |
| ES549526A0 (es) | 1987-11-01 |
| FI854781A0 (fi) | 1985-12-03 |
| ES556975A0 (es) | 1988-03-16 |
| PT81594A (de) | 1986-01-01 |
| YU186985A (en) | 1988-06-30 |
| GR852891B (es) | 1986-04-03 |
| PT81594B (de) | 1987-10-16 |
| ES8801705A1 (es) | 1988-02-16 |
| EP0184163A3 (de) | 1988-01-13 |
| NZ214422A (en) | 1988-02-12 |
| ES8800342A1 (es) | 1987-11-01 |
| FI854781A (fi) | 1986-06-05 |
| HU196454B (en) | 1988-11-28 |
| DE3444495A1 (de) | 1986-06-05 |
| SU1364241A3 (ru) | 1987-12-30 |
| ES556976A0 (es) | 1988-02-16 |
| JPS61181385A (ja) | 1986-08-14 |
| DK559585D0 (da) | 1985-12-03 |
| AU5070685A (en) | 1986-06-12 |
| EP0184163A2 (de) | 1986-06-11 |
| ZA859240B (en) | 1987-08-26 |
| HUT39775A (en) | 1986-10-29 |
| DK559585A (da) | 1986-06-05 |
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