ES8800342A1 - Procedimiento para preparar un vector de levadura de copias multiples,estables y recombinante - Google Patents
Procedimiento para preparar un vector de levadura de copias multiples,estables y recombinanteInfo
- Publication number
- ES8800342A1 ES8800342A1 ES549526A ES549526A ES8800342A1 ES 8800342 A1 ES8800342 A1 ES 8800342A1 ES 549526 A ES549526 A ES 549526A ES 549526 A ES549526 A ES 549526A ES 8800342 A1 ES8800342 A1 ES 8800342A1
- Authority
- ES
- Spain
- Prior art keywords
- preparation
- yeast vector
- multicopy yeast
- vectors
- multicopy
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/68—Stabilisation of the vector
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Metal Extraction Processes (AREA)
- Manufacturing Cores, Coils, And Magnets (AREA)
- Production Of Multi-Layered Print Wiring Board (AREA)
Abstract
PROCEDIMIENTO PARA PREPARAR UN VECTOR DE LEVADURA DE COPIAS MULTIPLES ESTABLE Y RECOMBINANTE. COMPRENDE: A) LINEALIZAR UN VECTOR CON ENDONUCLEASA DE RESTRICCION, PARA DAR UN ADN LINEAL; B) PURIFICAR Y DESFOSFORILAR AL ADN LINEAL; C) TRATAR AL VECTOR DE A) CON ENDUNUCLEASAS DE RESTRICCION, PARA FORMAR UN PROMOTOR REGULABLE; D) PURIFICAR EL PROMOTOR REGULABLE; E) UNIR ENTRE SI AL PROMOTOR REGULABLE CON EL ADN LINEAL MEDIANTE ADN-LIGASA, PARA OBTENER UN VECTOR DE LEVADURA DE COPIAS MULTIPLES. EL VECTOR TIENE UNA FUNCION ESTABILIZADORA Y ES UN CENTROMERO 3 DE LEVADURA QUE PRODUCE DEL PLASMIDO PBC3T1; EL PROMOTOR REGULABLE ES UN PROMOTOR ALCOHOL-DESHIDRIGLUCASA II Y PROCEDE DEL PLASMIDO PADH2BS Y LAS ENDONUCLEASAS DE RESTRICCION SON (BAMHI Y ECORV). 6
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19843444495 DE3444495A1 (de) | 1984-12-04 | 1984-12-04 | Herstellung eines multi-kopien-hefe-vektors |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES549526A0 ES549526A0 (es) | 1987-11-01 |
| ES8800342A1 true ES8800342A1 (es) | 1987-11-01 |
Family
ID=6252051
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES549526A Expired ES8800342A1 (es) | 1984-12-04 | 1985-12-03 | Procedimiento para preparar un vector de levadura de copias multiples,estables y recombinante |
| ES556975A Expired ES8802075A1 (es) | 1984-12-04 | 1986-07-23 | Procedimiento para la multiplicacion de un adn heterologo |
| ES556976A Expired ES8801705A1 (es) | 1984-12-04 | 1986-07-23 | Procedimiento para la expresion de una proteina heterologa |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES556975A Expired ES8802075A1 (es) | 1984-12-04 | 1986-07-23 | Procedimiento para la multiplicacion de un adn heterologo |
| ES556976A Expired ES8801705A1 (es) | 1984-12-04 | 1986-07-23 | Procedimiento para la expresion de una proteina heterologa |
Country Status (15)
| Country | Link |
|---|---|
| EP (1) | EP0184163A3 (es) |
| JP (1) | JPS61181385A (es) |
| AU (1) | AU5070685A (es) |
| DE (1) | DE3444495A1 (es) |
| DK (1) | DK559585A (es) |
| ES (3) | ES8800342A1 (es) |
| FI (1) | FI854781A (es) |
| GR (1) | GR852891B (es) |
| HU (1) | HU196454B (es) |
| NO (1) | NO854864L (es) |
| NZ (1) | NZ214422A (es) |
| PT (1) | PT81594B (es) |
| SU (1) | SU1364241A3 (es) |
| YU (1) | YU186985A (es) |
| ZA (1) | ZA859240B (es) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3605035A1 (de) * | 1986-02-18 | 1987-08-20 | Strahlen Umweltforsch Gmbh | Plasmid pws101, verfahren zu seiner gewinnung und seine verwendung |
| WO2016088824A1 (ja) * | 2014-12-03 | 2016-06-09 | 株式会社カネカ | セントロメアｄｎａ配列を含むベクター及びその用途 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL63035A (en) * | 1980-09-09 | 1985-05-31 | Univ California | Dnas capable of replication and stable mitotic maintenance in a host eukaryote,their preparation and eukaryotic cells comprising them |
-
1984
- 1984-12-04 DE DE19843444495 patent/DE3444495A1/de not_active Withdrawn
-
1985
- 1985-11-30 EP EP85115203A patent/EP0184163A3/de not_active Withdrawn
- 1985-12-02 YU YU01869/85A patent/YU186985A/xx unknown
- 1985-12-02 GR GR852891A patent/GR852891B/el unknown
- 1985-12-03 FI FI854781A patent/FI854781A/fi not_active IP Right Cessation
- 1985-12-03 PT PT81594A patent/PT81594B/pt unknown
- 1985-12-03 ZA ZA859240A patent/ZA859240B/xx unknown
- 1985-12-03 ES ES549526A patent/ES8800342A1/es not_active Expired
- 1985-12-03 NZ NZ214422A patent/NZ214422A/en unknown
- 1985-12-03 SU SU853991833A patent/SU1364241A3/ru active
- 1985-12-03 NO NO854864A patent/NO854864L/no unknown
- 1985-12-03 HU HU854619A patent/HU196454B/hu not_active IP Right Cessation
- 1985-12-03 AU AU50706/85A patent/AU5070685A/en not_active Abandoned
- 1985-12-03 DK DK559585A patent/DK559585A/da not_active Application Discontinuation
- 1985-12-04 JP JP60273181A patent/JPS61181385A/ja active Pending
-
1986
- 1986-07-23 ES ES556975A patent/ES8802075A1/es not_active Expired
- 1986-07-23 ES ES556976A patent/ES8801705A1/es not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| PT81594B (de) | 1987-10-16 |
| YU186985A (en) | 1988-06-30 |
| ES8802075A1 (es) | 1988-03-16 |
| ES556976A0 (es) | 1988-02-16 |
| ES549526A0 (es) | 1987-11-01 |
| ES556975A0 (es) | 1988-03-16 |
| NZ214422A (en) | 1988-02-12 |
| AU5070685A (en) | 1986-06-12 |
| JPS61181385A (ja) | 1986-08-14 |
| SU1364241A3 (ru) | 1987-12-30 |
| EP0184163A3 (de) | 1988-01-13 |
| FI854781A0 (fi) | 1985-12-03 |
| DK559585A (da) | 1986-06-05 |
| EP0184163A2 (de) | 1986-06-11 |
| ES8801705A1 (es) | 1988-02-16 |
| PT81594A (de) | 1986-01-01 |
| ZA859240B (en) | 1987-08-26 |
| HU196454B (en) | 1988-11-28 |
| DE3444495A1 (de) | 1986-06-05 |
| DK559585D0 (da) | 1985-12-03 |
| HUT39775A (en) | 1986-10-29 |
| GR852891B (es) | 1986-04-03 |
| NO854864L (no) | 1986-06-05 |
| FI854781A (fi) | 1986-06-05 |
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