ES8606651A1 - Un metodo para detectar una secuencia de polinucleotidos en un analito - Google Patents

Un metodo para detectar una secuencia de polinucleotidos en un analito

Info

Publication number
ES8606651A1
ES8606651A1 ES542287A ES542287A ES8606651A1 ES 8606651 A1 ES8606651 A1 ES 8606651A1 ES 542287 A ES542287 A ES 542287A ES 542287 A ES542287 A ES 542287A ES 8606651 A1 ES8606651 A1 ES 8606651A1
Authority
ES
Spain
Prior art keywords
vivo
polynucleotide sequences
vivo labelling
labelled polynucleotides
labelling
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES542287A
Other languages
English (en)
Other versions
ES542287A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Enzo Biochem Inc
Original Assignee
Enzo Biochem Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Enzo Biochem Inc filed Critical Enzo Biochem Inc
Publication of ES8606651A1 publication Critical patent/ES8606651A1/es
Publication of ES542287A0 publication Critical patent/ES542287A0/es
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

METODO DE DETECCION DE SECUENCIAS DE POLINUCLEOTIDOS EN ANALITOS. COMPRENDE EL MARCADO DE UNA SECUENCIA DE POLINUCLEOTIDOS MEDIANTE EL CULTIVO DE UN ORGANISMO HOSPEDANTE CON LA MISMA SECUENCIA QUE EL QUE SE DESEA MARCAR, EN PRESENCIA DE UNA FUENTE DE UN MARCADOR CON UNA SECUENCIA DE POLINUCLEOTIDOS, QUE EXPRESA UN PRODUCTO POR CULTIVO DEL ORGANISMO HOSPEDANTE, QUE MARCA LA SECUENCIA DESEADA CUANDO SE REPLICA EN EL HOSPEDANTE CULTIVADO, Y DE UNA BASE, NUCLEOSIDO O NUCLEOTIDO, REQUERIDO POR EL HOSPEDANTE O SU VARIANTE PARA SU CRECIMIENTO; HIBRIDACION DE DICHA SECUENCIA DE POLINUCLEOTIDOS MARCADA A LA SECUENCIA COMPLEMENTARIA DEL ANALITO; Y DETECCION DE ESTA HIBRIDACION. TIENE APLICACIONES EN LABORATORIOS INDUSTRIALES Y MEDICOS PARA LA DETECCION DE ANALITOS.
ES542287A 1983-07-05 1985-04-16 Un metodo para detectar una secuencia de polinucleotidos en un analito Expired ES8606651A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US51097583A 1983-07-05 1983-07-05

Publications (2)

Publication Number Publication Date
ES8606651A1 true ES8606651A1 (es) 1986-04-01
ES542287A0 ES542287A0 (es) 1986-04-01

Family

ID=24032961

Family Applications (2)

Application Number Title Priority Date Filing Date
ES534014A Expired ES8606229A1 (es) 1983-07-05 1984-07-04 Un procedimiento para marcar in vivo secuencias de polinucleotidos
ES542287A Expired ES8606651A1 (es) 1983-07-05 1985-04-16 Un metodo para detectar una secuencia de polinucleotidos en un analito

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES534014A Expired ES8606229A1 (es) 1983-07-05 1984-07-04 Un procedimiento para marcar in vivo secuencias de polinucleotidos

Country Status (10)

Country Link
EP (1) EP0133473B1 (es)
JP (2) JP2540482B2 (es)
AT (1) ATE103327T1 (es)
AU (1) AU581620B2 (es)
CA (1) CA1338856C (es)
DE (1) DE3486290T2 (es)
DK (1) DK328084A (es)
ES (2) ES8606229A1 (es)
IL (1) IL72302A0 (es)
NO (1) NO842720L (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE45385T1 (de) * 1984-05-15 1989-08-15 Smithkline Beckman Corp Polynucleotid-hybridisierungsprobe.
CA1277931C (en) * 1984-06-05 1990-12-18 Shimon Ulitzur Detection and/or identification of microorganisms in a test sample usingbioluminescence or other exogenous genetically-introduced marker
NO870613L (no) * 1986-03-05 1987-09-07 Molecular Diagnostics Inc Deteksjon av mikroorganismer i en prŸve inneholdende nukleinsyre.
FR2596773B1 (fr) * 1986-04-04 1988-06-24 Pasteur Institut Sonde monocatenaire marquee, non radioactive, procede pour sa fabrication et procede de detection d'une sequence nucleotidique determinee a l'aide de cette sonde
SE455600B (sv) * 1986-11-27 1988-07-25 Svalof Ab Forfarande for detektering av vextvirus och dess tillempning for detektering av rodsotvirus och potatisvirus
AU601021B2 (en) * 1987-03-11 1990-08-30 Molecular Diagnostics, Inc. Assay for necleic acid sequences in a sample
GB2209754A (en) * 1987-09-17 1989-05-24 Thomas Lee Mattson Polyaldehydic polynucleotides in use as probes,their preparation and use
US6326136B1 (en) 1988-04-01 2001-12-04 Digene Corporation Macromolecular conjugate made using unsaturated aldehydes
AU2945792A (en) * 1991-11-25 1993-06-28 Keygene N.V. A novel pcr method with a single primer for nucleic acid analysis
WO1997008345A1 (en) * 1995-08-30 1997-03-06 New York Medical College Methods for labeling dna ends with halogenated nucleotides and detecting same with antibodies
DE19850594A1 (de) * 1998-11-03 2000-05-04 Biochip Technologies Gmbh Verfahren zur Markierung von Nukleinsäuren
DE102006038686A1 (de) * 2006-08-17 2008-02-21 Forschungszentrum Karlsruhe Gmbh Verfahren zur nicht radioaktiven Markierung von Ribonukleinsäure

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1223831A (en) * 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
US4556643A (en) * 1982-07-26 1985-12-03 Agracetus Assay method and probe for polynucleotide sequences
IL70765A (en) * 1983-01-27 1988-07-31 Enzo Biochem Inc Substrates containing non-radioactive chemically-labeled polynucleotides and methods using them
CA1228811A (en) * 1983-05-05 1987-11-03 Robert G. Pergolizzi Assay method utilizing polynucleotide sequences

Also Published As

Publication number Publication date
ES8606229A1 (es) 1986-04-01
AU3020584A (en) 1985-01-10
CA1338856C (en) 1997-01-21
IL72302A0 (en) 1984-11-30
JPH08224084A (ja) 1996-09-03
JP2607058B2 (ja) 1997-05-07
ES542287A0 (es) 1986-04-01
EP0133473B1 (en) 1994-03-23
DE3486290D1 (de) 1994-04-28
JP2540482B2 (ja) 1996-10-02
JPS6076667A (ja) 1985-05-01
DE3486290T2 (de) 1994-07-14
AU581620B2 (en) 1989-03-02
EP0133473A2 (en) 1985-02-27
NO842720L (no) 1985-01-07
DK328084D0 (da) 1984-07-04
DK328084A (da) 1985-01-06
EP0133473A3 (en) 1987-02-04
ATE103327T1 (de) 1994-04-15
ES534014A0 (es) 1986-04-01

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20050924