ES8606229A1 - Un procedimiento para marcar in vivo secuencias de polinucleotidos - Google Patents
Un procedimiento para marcar in vivo secuencias de polinucleotidosInfo
- Publication number
- ES8606229A1 ES8606229A1 ES534014A ES534014A ES8606229A1 ES 8606229 A1 ES8606229 A1 ES 8606229A1 ES 534014 A ES534014 A ES 534014A ES 534014 A ES534014 A ES 534014A ES 8606229 A1 ES8606229 A1 ES 8606229A1
- Authority
- ES
- Spain
- Prior art keywords
- vivo
- polynucleotide sequences
- vivo labelling
- labelled polynucleotides
- labelling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Hematology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Saccharide Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
METODO PARA EL MARCADO IN VIVO DE SECUENCIAS DE POLINUCLEOTIDOS. COMPRENDE EL CULTIVO DE UN ORGANISMO HOSPEDANTE, QUE TIENE LA SECUENCIA DE POLINUCLEOTIDOS QUE SE QUIERE MARCAR, EN PRESENCIA DE UNA FUENTE DE MARCADOR ELEGIDO DEL GRUPO QUE CONSISTE EN UNA SECUENCIA DE POLINUCLEOTIDOS QUE EXPRESA UN PRODUCTO POR CULTIVO DEL HOSPEDANTE MARCADO DE LA SECUENCIA DE POLINUCLEOTIDOS DESEADA CUANDO SE REPLICA EN EL HOSPEDANTE CULTIVADO, Y EN PRESENCIA DE UNA BASE, NUCLEOXIDO, NUCLEOTIDO O PRECURSOR DE ELLOS, QUE EL HOSPEDANTE O UNA DE SUS VARIANTES NECESITA PARA SU CRECIMIENTO Y QUE LLEVA EL MARCADOR DESEADO. ESTAS SECUENCIAS DE NUCLEOTIDOS MARCADOS TIENEN APLICACIONES EN LABORATORIO, MEDICINA E INDUSTRIA EN LOS QUE SE DESEA LA DETECCION DE ANALITOS.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US51097583A | 1983-07-05 | 1983-07-05 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES534014A0 ES534014A0 (es) | 1986-04-01 |
ES8606229A1 true ES8606229A1 (es) | 1986-04-01 |
Family
ID=24032961
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES534014A Expired ES8606229A1 (es) | 1983-07-05 | 1984-07-04 | Un procedimiento para marcar in vivo secuencias de polinucleotidos |
ES542287A Expired ES8606651A1 (es) | 1983-07-05 | 1985-04-16 | Un metodo para detectar una secuencia de polinucleotidos en un analito |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES542287A Expired ES8606651A1 (es) | 1983-07-05 | 1985-04-16 | Un metodo para detectar una secuencia de polinucleotidos en un analito |
Country Status (10)
Country | Link |
---|---|
EP (1) | EP0133473B1 (es) |
JP (2) | JP2540482B2 (es) |
AT (1) | ATE103327T1 (es) |
AU (1) | AU581620B2 (es) |
CA (1) | CA1338856C (es) |
DE (1) | DE3486290T2 (es) |
DK (1) | DK328084A (es) |
ES (2) | ES8606229A1 (es) |
IL (1) | IL72302A0 (es) |
NO (1) | NO842720L (es) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0172153B1 (en) * | 1984-05-15 | 1989-08-09 | Smithkline Beckman Corporation | Polynucleotide hybridization probes |
CA1277931C (en) * | 1984-06-05 | 1990-12-18 | Shimon Ulitzur | Detection and/or identification of microorganisms in a test sample usingbioluminescence or other exogenous genetically-introduced marker |
NO870613L (no) * | 1986-03-05 | 1987-09-07 | Molecular Diagnostics Inc | Deteksjon av mikroorganismer i en prve inneholdende nukleinsyre. |
FR2596773B1 (fr) * | 1986-04-04 | 1988-06-24 | Pasteur Institut | Sonde monocatenaire marquee, non radioactive, procede pour sa fabrication et procede de detection d'une sequence nucleotidique determinee a l'aide de cette sonde |
SE455600B (sv) * | 1986-11-27 | 1988-07-25 | Svalof Ab | Forfarande for detektering av vextvirus och dess tillempning for detektering av rodsotvirus och potatisvirus |
AU601021B2 (en) * | 1987-03-11 | 1990-08-30 | Molecular Diagnostics, Inc. | Assay for necleic acid sequences in a sample |
GB2209754A (en) * | 1987-09-17 | 1989-05-24 | Thomas Lee Mattson | Polyaldehydic polynucleotides in use as probes,their preparation and use |
US6326136B1 (en) | 1988-04-01 | 2001-12-04 | Digene Corporation | Macromolecular conjugate made using unsaturated aldehydes |
WO1993011261A1 (en) * | 1991-11-25 | 1993-06-10 | Keygene N.V. | A novel pcr method with a single primer for nucleic acid analysis |
US5912126A (en) * | 1995-08-30 | 1999-06-15 | Darzynkiewicz; Zbigniew | Methods for labeling DNA ends with halogenated nucleotides and detecting same with antibodies |
DE19850594A1 (de) * | 1998-11-03 | 2000-05-04 | Biochip Technologies Gmbh | Verfahren zur Markierung von Nukleinsäuren |
DE102006038686A1 (de) * | 2006-08-17 | 2008-02-21 | Forschungszentrum Karlsruhe Gmbh | Verfahren zur nicht radioaktiven Markierung von Ribonukleinsäure |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1223831A (en) * | 1982-06-23 | 1987-07-07 | Dean Engelhardt | Modified nucleotides, methods of preparing and utilizing and compositions containing the same |
US4556643A (en) * | 1982-07-26 | 1985-12-03 | Agracetus | Assay method and probe for polynucleotide sequences |
CA1309672C (en) * | 1983-01-27 | 1992-11-03 | Jannis G. Stavrianopoulos | Methods and structures employing non-radioactive chemically-labeled polynucleotide probes |
CA1228811A (en) * | 1983-05-05 | 1987-11-03 | Robert G. Pergolizzi | Assay method utilizing polynucleotide sequences |
-
1984
- 1984-06-28 CA CA000457804A patent/CA1338856C/en not_active Expired - Lifetime
- 1984-07-03 AU AU30205/84A patent/AU581620B2/en not_active Ceased
- 1984-07-04 DE DE3486290T patent/DE3486290T2/de not_active Expired - Lifetime
- 1984-07-04 EP EP84107772A patent/EP0133473B1/en not_active Expired - Lifetime
- 1984-07-04 JP JP59137381A patent/JP2540482B2/ja not_active Expired - Lifetime
- 1984-07-04 ES ES534014A patent/ES8606229A1/es not_active Expired
- 1984-07-04 AT AT84107772T patent/ATE103327T1/de not_active IP Right Cessation
- 1984-07-04 NO NO842720A patent/NO842720L/no unknown
- 1984-07-04 DK DK328084A patent/DK328084A/da not_active Application Discontinuation
- 1984-07-05 IL IL72302A patent/IL72302A0/xx unknown
-
1985
- 1985-04-16 ES ES542287A patent/ES8606651A1/es not_active Expired
-
1995
- 1995-12-20 JP JP7332416A patent/JP2607058B2/ja not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
EP0133473A3 (en) | 1987-02-04 |
AU581620B2 (en) | 1989-03-02 |
JPS6076667A (ja) | 1985-05-01 |
EP0133473A2 (en) | 1985-02-27 |
ES542287A0 (es) | 1986-04-01 |
DK328084A (da) | 1985-01-06 |
CA1338856C (en) | 1997-01-21 |
DE3486290T2 (de) | 1994-07-14 |
NO842720L (no) | 1985-01-07 |
AU3020584A (en) | 1985-01-10 |
JP2607058B2 (ja) | 1997-05-07 |
EP0133473B1 (en) | 1994-03-23 |
ES534014A0 (es) | 1986-04-01 |
DE3486290D1 (de) | 1994-04-28 |
DK328084D0 (da) | 1984-07-04 |
JPH08224084A (ja) | 1996-09-03 |
ES8606651A1 (es) | 1986-04-01 |
ATE103327T1 (de) | 1994-04-15 |
IL72302A0 (en) | 1984-11-30 |
JP2540482B2 (ja) | 1996-10-02 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD1A | Patent lapsed |
Effective date: 20050711 |