ES474852A1 - Un metodo para la expresion,en un huesped bacteriano trans- formado de un polipeptido heterologo con respecto al huespeden su estado no transformado - Google Patents
Un metodo para la expresion,en un huesped bacteriano trans- formado de un polipeptido heterologo con respecto al huespeden su estado no transformadoInfo
- Publication number
- ES474852A1 ES474852A1 ES474852A ES474852A ES474852A1 ES 474852 A1 ES474852 A1 ES 474852A1 ES 474852 A ES474852 A ES 474852A ES 474852 A ES474852 A ES 474852A ES 474852 A1 ES474852 A1 ES 474852A1
- Authority
- ES
- Spain
- Prior art keywords
- plasmid
- render
- bacterial host
- polypeptide expression
- transforming bacterial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Plant Pathology (AREA)
- Endocrinology (AREA)
- Microbiology (AREA)
- Diabetes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
Abstract
Un método para la expresión en un huésped bacteriano transformado, de un polipéptido heterólogo con respecto al huésped en su estado no transformado cuyo método comprende: a) obtener una cantidad de huésped transformante, elemento individuales del mismo que contengan uno o más plásmidos que comprenden: 1) un regulador homólogo al huésped bacteriano en su estado no transformado y 2) en fase de lectura con el regulador, una codificación de inserción ADN para la secuencia de aminoácidos de un polipéptido heterólogo , de tal manera que las bacterias transformadas por el plásmido son capaces de expresar la secuencia de aminoácidos en forma recuperable, b) separar el huesped transformante en un medio adecuado para su crecimiento y c)provocar la expresión de dicho polipéptido.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US84969277A | 1977-11-08 | 1977-11-08 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES474852A1 true ES474852A1 (es) | 1979-12-01 |
Family
ID=25306290
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES474852A Expired ES474852A1 (es) | 1977-11-08 | 1978-11-06 | Un metodo para la expresion,en un huesped bacteriano trans- formado de un polipeptido heterologo con respecto al huespeden su estado no transformado |
Country Status (30)
Country | Link |
---|---|
EP (1) | EP0001929B1 (es) |
JP (2) | JPS54163600A (es) |
AT (1) | AT373279B (es) |
BG (1) | BG38165A3 (es) |
CA (1) | CA1215921A (es) |
CH (1) | CH662128A5 (es) |
DD (1) | DD142561A5 (es) |
DE (2) | DE2862495D1 (es) |
DK (1) | DK173092B1 (es) |
ES (1) | ES474852A1 (es) |
FI (1) | FI783365A (es) |
GB (1) | GB2007676B (es) |
GR (1) | GR71906B (es) |
HK (1) | HK87184A (es) |
IE (1) | IE47891B1 (es) |
IL (1) | IL55892A (es) |
IT (1) | IT1100477B (es) |
KE (1) | KE3447A (es) |
MW (1) | MW3378A1 (es) |
MY (1) | MY8500762A (es) |
NL (2) | NL7811042A (es) |
NO (1) | NO783722L (es) |
NZ (1) | NZ188836A (es) |
PH (1) | PH16937A (es) |
PL (1) | PL210787A1 (es) |
PT (1) | PT68755A (es) |
SE (1) | SE501005C2 (es) |
SG (1) | SG56884G (es) |
YU (2) | YU257278A (es) |
ZA (1) | ZA786304B (es) |
Families Citing this family (64)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4171824A (en) * | 1977-05-23 | 1979-10-23 | Foster Edwin E | Bicyle |
US4565785A (en) * | 1978-06-08 | 1986-01-21 | The President And Fellows Of Harvard College | Recombinant DNA molecule |
US4411994A (en) * | 1978-06-08 | 1983-10-25 | The President And Fellows Of Harvard College | Protein synthesis |
IE48385B1 (en) * | 1978-08-11 | 1984-12-26 | Univ California | Synthesis of a eucaryotic protein by a microorganism |
FR2480779B2 (fr) * | 1979-08-30 | 1986-07-18 | Anvar | Vecteur contenant une sequence nucleotidique de l'antigene de surface du virus de l'hepatite b et procede de fabrication d'une molecule immunogene mettant en oeuvre ce vecteur |
US6268122B1 (en) | 1978-12-22 | 2001-07-31 | Biogen, Inc. | Recombinant DNA molecules and their method of production |
ZA802992B (en) * | 1979-06-01 | 1981-10-28 | Univ California | Human pre-growth hormone |
FR2458584A1 (fr) * | 1979-06-08 | 1981-01-02 | Pasteur Institut | Vecteurs pour le transfert et l'expression de materiel genetique dans une cellule eucaryote et procede pour la production d'une proteine determinee dans des cellules eucaryotes |
US4342832A (en) * | 1979-07-05 | 1982-08-03 | Genentech, Inc. | Method of constructing a replicable cloning vehicle having quasi-synthetic genes |
GR70279B (es) * | 1979-09-12 | 1982-09-03 | Univ California | |
CY1346A (en) * | 1980-01-08 | 1987-01-16 | Biogen Nv | Dna sequences,recombinant dna molecules and processes for producing human interferon-like polypeptides |
US6835557B1 (en) | 1980-01-08 | 2004-12-28 | Biogen, Inc. | DNA sequences, recombinant DNA molecules and processes for producing human interferon-like polypeptides |
US4443539A (en) * | 1980-02-05 | 1984-04-17 | The Upjohn Company | Process for preparing bovine growth hormone |
US6455275B1 (en) | 1980-02-25 | 2002-09-24 | The Trustees Of Columbia University In The City Of New York | DNA construct for producing proteinaceous materials in eucaryotic cells |
US4769326A (en) * | 1980-02-29 | 1988-09-06 | The Regents Of The University Of California | Expression linkers |
CA1200773A (en) * | 1980-02-29 | 1986-02-18 | William J. Rutter | Expression linkers |
US4350764A (en) * | 1980-03-10 | 1982-09-21 | The Regents Of The University Of California | Microbiological synthesis of beta endorphin |
ZA811368B (en) * | 1980-03-24 | 1982-04-28 | Genentech Inc | Bacterial polypedtide expression employing tryptophan promoter-operator |
CA1202581A (en) * | 1980-03-27 | 1986-04-01 | Saran A. Narang | Adaptor molecules for dna and their application to synthesis of gene-derived products |
DE3177213D1 (de) | 1980-04-03 | 1990-10-18 | Biogen Inc | Dns-sequenzen, rekombinante dns-molekuele und verfahren zur herstellung von dem menschlichen fibroblast-interferon. |
US6610830B1 (en) | 1980-07-01 | 2003-08-26 | Hoffman-La Roche Inc. | Microbial production of mature human leukocyte interferons |
IE52122B1 (en) * | 1980-08-25 | 1987-06-24 | Univ California | Somatostatin or somatostatin precursors |
IE52755B1 (en) * | 1980-08-26 | 1988-02-17 | Univ California | Bovine pre-growth and growth hormone |
US6692941B1 (en) | 1980-08-26 | 2004-02-17 | The Regents Of The University Of California | Bovine growth hormone |
FR2490675B1 (fr) * | 1980-09-25 | 1985-11-15 | Genentech Inc | Production microbienne d'interferon de fibroplaste humain |
US4506013A (en) * | 1980-10-03 | 1985-03-19 | Eli Lilly And Company | Stabilizing and selecting recombinant DNA host cells |
FI64813C (fi) * | 1980-12-31 | 1984-01-10 | Ilkka Antero Palva | Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer |
US4624926A (en) * | 1981-01-02 | 1986-11-25 | The Research Foundation Of State University Of New York | Novel cloning vehicles for polypeptide expression in microbial hosts |
US4666836A (en) * | 1981-01-02 | 1987-05-19 | The Research Foundation Of State University Of New York | Novel cloning vehicles for polypeptide expression in microbial hosts |
NZ199391A (en) * | 1981-01-02 | 1985-12-13 | Genentech Inc | Chimeric polypeptides comprising a proinsulin sequence,and preparation by recombinant dna technique;production of human insulin |
NZ199722A (en) * | 1981-02-25 | 1985-12-13 | Genentech Inc | Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain |
JPS57181098A (en) * | 1981-04-30 | 1982-11-08 | Japan Found Cancer | Novel recombinant dna |
JPS58996A (ja) * | 1981-06-01 | 1983-01-06 | ザ・ボ−ド・オブ・トラステイ−ズ・ミシガンステ−トユニバ−シテイ− | 細菌中での牛成長ホルモン遺伝子のクロ−ン化 |
US5254463A (en) * | 1981-09-18 | 1993-10-19 | Genentech, Inc. | Method for expression of bovine growth hormone |
US4650761A (en) * | 1981-11-27 | 1987-03-17 | Eli Lilly And Company | Method for stabilizing and selecting recombinant DNA containing host cell |
US4863855A (en) * | 1982-05-14 | 1989-09-05 | The Research Foundation Of State University Of New York | Novel cloning vehicles for polypeptide expression in microbial hosts |
US4530904A (en) * | 1982-09-03 | 1985-07-23 | Eli Lilly And Company | Method for conferring bacteriophage resistance to bacteria |
DE3378638D1 (de) * | 1982-10-25 | 1989-01-12 | Monsanto Co | Reca promoter dependent polypeptide production |
ATE255162T1 (de) * | 1983-01-13 | 2003-12-15 | Max Planck Gesellschaft | Transgene dicotyledone pflanzenzellen und pflanzen |
US4888286A (en) * | 1984-02-06 | 1989-12-19 | Creative Biomolecules, Inc. | Production of gene and protein analogs through synthetic gene design using double stranded synthetic oligonucleotides |
EP0159123B1 (en) | 1984-03-06 | 1992-01-15 | Eli Lilly And Company | Vectors for expressing bovine growth hormone derivatives |
GB8407044D0 (en) * | 1984-03-19 | 1984-04-26 | Fujisawa Pharmaceutical Co | Producing human insulin |
DK108685A (da) * | 1984-03-19 | 1985-09-20 | Fujisawa Pharmaceutical Co | Vaekstfaktor i |
IT1198443B (it) * | 1984-03-30 | 1988-12-21 | Serono Ist Farm | Espressione genetica di polipeptidi ibridi contenenti la sequenza della somatostatina |
US4745179A (en) * | 1984-04-02 | 1988-05-17 | Fujisawa Pharmaceutical Co., Ltd. | 59 Valine insulin-like growth factor I and process for production thereof |
CA1213537A (en) * | 1984-05-01 | 1986-11-04 | Canadian Patents And Development Limited - Societe Canadienne Des Brevets Et D'exploitation Limitee | Polypeptide expression method |
NL8401781A (nl) * | 1984-06-04 | 1986-01-02 | Rijksuniversiteit Leiden En Pr | Werkwijze voor het activeren van promotoren van bacterien. |
US5489529A (en) * | 1984-07-19 | 1996-02-06 | De Boer; Herman A. | DNA for expression of bovine growth hormone |
EP0206769B2 (en) * | 1985-06-20 | 1999-11-10 | Fujisawa Pharmaceutical Co., Ltd. | A process for the production of alpha-human atrial natriuretic polypeptide |
FR2594846B1 (fr) * | 1986-02-21 | 1989-10-20 | Genetica | Procede de preparation de la serum albumine humaine mature |
US5017692A (en) * | 1986-09-04 | 1991-05-21 | Schering Corporation | Truncated human interleukin-a alpha |
US5254678A (en) * | 1987-12-15 | 1993-10-19 | Gene Shears Pty. Limited | Ribozymes |
DE4226971C2 (de) * | 1992-08-14 | 1997-01-16 | Widmar Prof Dr Tanner | Modifizierte Pilzzellen und Verfahren zur Herstellung rekombinanter Produkte |
EP2332957B1 (en) * | 1996-02-09 | 2015-04-08 | Cornell Research Foundation, Inc. | Detection of nucleic and acid sequence differences using the ligase detection reaction with addressable arrays |
US6852487B1 (en) | 1996-02-09 | 2005-02-08 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
DE19729769A1 (de) | 1997-07-11 | 1999-01-14 | Cardiogene Gentherapeutische S | Transfektionssystem, seine Herstellung und Verwendung in der somatischen Gentherapie |
DE19905501B4 (de) | 1999-02-10 | 2005-05-19 | MediGene AG, Gesellschaft für molekularbiologische Kardiologie und Onkologie | Verfahren zur Herstellung eines rekombinanten Adeno-assoziierten Virus, geeignete Mittel hierzu sowie Verwendung zur Herstellung eines Arzneimittels |
DE102009001806A1 (de) | 2009-03-24 | 2010-09-30 | Evonik Degussa Gmbh | Prepregs und daraus bei niedriger Temperatur hergestellte Formkörper |
DE102009001793A1 (de) | 2009-03-24 | 2010-10-07 | Evonik Degussa Gmbh | Prepregs und daraus hergestellte Formkörper |
DE102010029355A1 (de) | 2010-05-27 | 2011-12-01 | Evonik Degussa Gmbh | Verfahren zur Herstellung von lagerstabilen Polyurethan-Prepregs und daraus hergestellte Formkörper |
DE102010041247A1 (de) | 2010-09-23 | 2012-03-29 | Evonik Degussa Gmbh | Verfahren zur Herstellung von lagerstabilen Polyurethan-Prepregs und daraus hergestellte Formkörper aus Polyurethanzusammensetzung in Lösung |
DE102011006163A1 (de) | 2011-03-25 | 2012-09-27 | Evonik Degussa Gmbh | Lagerstabile Polyurethan-Prepregs und daraus hergestellte Formkörper aus Polyurethanzusammensetzung mit flüssigen Harzkomponenten |
EP3330311B1 (de) | 2016-12-02 | 2021-05-05 | Evonik Operations GmbH | Lagerstabile 1k-polyurethan-prepregs und daraus hergestellte formkörper aus polyurethanzusammensetzung |
BR112020023538A2 (pt) | 2018-05-22 | 2021-05-04 | Katherine Konstantin Sizov | biossensor de receptor de etileno |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1521032A (en) * | 1974-08-08 | 1978-08-09 | Ici Ltd | Biological treatment |
US4237224A (en) * | 1974-11-04 | 1980-12-02 | Board Of Trustees Of The Leland Stanford Jr. University | Process for producing biologically functional molecular chimeras |
US4468464A (en) * | 1974-11-04 | 1984-08-28 | The Board Of Trustees Of The Leland Stanford Junior University | Biologically functional molecular chimeras |
US4082613A (en) * | 1976-04-23 | 1978-04-04 | The Regents Of The University Of Minnesota | Process for the production of insulin by genetically transformed fungal cells |
DE2712615A1 (de) * | 1977-03-18 | 1978-09-21 | Max Planck Gesellschaft | Verfahren zur herstellung von filamentoesen phagen als vektor fuer synthetische rekombinate |
US4366246A (en) * | 1977-11-08 | 1982-12-28 | Genentech, Inc. | Method for microbial polypeptide expression |
IE48385B1 (en) * | 1978-08-11 | 1984-12-26 | Univ California | Synthesis of a eucaryotic protein by a microorganism |
US4431739A (en) * | 1979-11-05 | 1984-02-14 | Genentech, Inc. | Transformant bacterial culture capable of expressing heterologous protein |
-
1978
- 1978-11-06 AT AT0793478A patent/AT373279B/de active
- 1978-11-06 MW MW33/78A patent/MW3378A1/xx unknown
- 1978-11-06 YU YU02572/78A patent/YU257278A/xx unknown
- 1978-11-06 FI FI783365A patent/FI783365A/fi not_active Application Discontinuation
- 1978-11-06 GB GB7843446A patent/GB2007676B/en not_active Expired
- 1978-11-06 DK DK197804937A patent/DK173092B1/da not_active IP Right Cessation
- 1978-11-06 ES ES474852A patent/ES474852A1/es not_active Expired
- 1978-11-06 GR GR57590A patent/GR71906B/el unknown
- 1978-11-06 IL IL55892A patent/IL55892A/xx unknown
- 1978-11-06 BG BG7841308A patent/BG38165A3/xx unknown
- 1978-11-06 NO NO783722A patent/NO783722L/no unknown
- 1978-11-06 EP EP78300596A patent/EP0001929B1/en not_active Expired
- 1978-11-06 JP JP13721578A patent/JPS54163600A/ja active Granted
- 1978-11-06 DE DE7878300596T patent/DE2862495D1/de not_active Expired
- 1978-11-06 CH CH11416/78A patent/CH662128A5/de not_active IP Right Cessation
- 1978-11-06 CA CA000315819A patent/CA1215921A/en not_active Expired
- 1978-11-06 NL NL7811042A patent/NL7811042A/xx not_active Application Discontinuation
- 1978-11-06 NZ NZ188836A patent/NZ188836A/xx unknown
- 1978-11-06 DE DE19782848053 patent/DE2848053A1/de not_active Withdrawn
- 1978-11-06 SE SE7811461A patent/SE501005C2/sv unknown
- 1978-11-06 IE IE2193/78A patent/IE47891B1/en unknown
- 1978-11-07 PT PT68755A patent/PT68755A/pt unknown
- 1978-11-07 DD DD78208918A patent/DD142561A5/de unknown
- 1978-11-07 IT IT29518/78A patent/IT1100477B/it active Protection Beyond IP Right Term
- 1978-11-08 ZA ZA00786304A patent/ZA786304B/xx unknown
- 1978-11-08 PH PH21775A patent/PH16937A/en unknown
- 1978-11-08 PL PL21078778A patent/PL210787A1/xx unknown
-
1983
- 1983-12-02 YU YU02362/83A patent/YU236283A/xx unknown
-
1984
- 1984-01-08 HK HK871/84A patent/HK87184A/xx unknown
- 1984-08-13 SG SG568/84A patent/SG56884G/en unknown
- 1984-09-13 KE KE3447A patent/KE3447A/xx unknown
-
1985
- 1985-12-30 MY MY762/85A patent/MY8500762A/xx unknown
-
1993
- 1993-06-29 NL NL930113C patent/NL930113I1/nl unknown
-
1994
- 1994-11-28 JP JP6292816A patent/JP2694840B2/ja not_active Expired - Lifetime
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