ES470653A1 - Procedimiento para el enriquecimiento de una glicoproteina - Google Patents
Procedimiento para el enriquecimiento de una glicoproteinaInfo
- Publication number
- ES470653A1 ES470653A1 ES470653A ES470653A ES470653A1 ES 470653 A1 ES470653 A1 ES 470653A1 ES 470653 A ES470653 A ES 470653A ES 470653 A ES470653 A ES 470653A ES 470653 A1 ES470653 A1 ES 470653A1
- Authority
- ES
- Spain
- Prior art keywords
- glycoprotein
- alpha
- antiserum
- molecular weight
- determined
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000003886 Glycoproteins Human genes 0.000 title abstract 3
- 108090000288 Glycoproteins Proteins 0.000 title abstract 3
- 238000004519 manufacturing process Methods 0.000 title 1
- MSWZFWKMSRAUBD-UHFFFAOYSA-N 2-Amino-2-Deoxy-Hexose Chemical compound NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 abstract 1
- CERZMXAJYMMUDR-QBTAGHCHSA-N 5-amino-3,5-dideoxy-D-glycero-D-galacto-non-2-ulopyranosonic acid Chemical compound N[C@@H]1[C@@H](O)CC(O)(C(O)=O)O[C@H]1[C@H](O)[C@H](O)CO CERZMXAJYMMUDR-QBTAGHCHSA-N 0.000 abstract 1
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 abstract 1
- PNNNRSAQSRJVSB-SLPGGIOYSA-N Fucose Natural products C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C=O PNNNRSAQSRJVSB-SLPGGIOYSA-N 0.000 abstract 1
- SHZGCJCMOBCMKK-DHVFOXMCSA-N L-fucopyranose Chemical compound C[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O SHZGCJCMOBCMKK-DHVFOXMCSA-N 0.000 abstract 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 abstract 1
- 150000001371 alpha-amino acids Chemical class 0.000 abstract 1
- 235000008206 alpha-amino acids Nutrition 0.000 abstract 1
- 230000008033 biological extinction Effects 0.000 abstract 1
- 150000001720 carbohydrates Chemical class 0.000 abstract 1
- 150000002402 hexoses Chemical class 0.000 abstract 1
- 230000001900 immune effect Effects 0.000 abstract 1
- CERZMXAJYMMUDR-UHFFFAOYSA-N neuraminic acid Natural products NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO CERZMXAJYMMUDR-UHFFFAOYSA-N 0.000 abstract 1
- 229920002401 polyacrylamide Polymers 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000036647 reaction Effects 0.000 abstract 1
- 238000004062 sedimentation Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4715—Pregnancy proteins, e.g. placenta proteins, alpha-feto-protein, pregnancy specific beta glycoprotein
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/827—Proteins from mammals or birds
- Y10S530/829—Blood
- Y10S530/83—Plasma; serum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/827—Proteins from mammals or birds
- Y10S530/85—Reproductive organs or embryos
- Y10S530/851—Placenta; amniotic fluid
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Pregnancy & Childbirth (AREA)
- Gynecology & Obstetrics (AREA)
- Immunology (AREA)
- Reproductive Health (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Procedimiento para el enriquecimiento de una glicoproteína que se distingue por a) una fracción de proteínas de 75 + _ 6%; b) una fracción de hidratos de carbono de 24,6 + _ 5,2%, de ellos hexosas 8,9 + _ , hexosamina acetilada en N 7,1 + _ 1,5% , fucosa 0,2% , ácido neurámico acetilado en N 8,4 + _ 1,5% , c) un coeficiente de sedimentación S(sub 20w) de 2,5 + _ 0,3 S ; d) un peso molecular determinado en la ultracentrífuga de 35000 + _ 5000; e) un punto isoeléctrico de pH 3,4 + _ 0,4 ; f) un coeficiente de extinción E (sup 1%, sub 1 cm) (280 nm) de 1,9 + _ 0,3 ; g) una movilidad electroforética en el margen entre [alfa (sub 1)-globulinas] y [alfa (sub 2)-globulinas; y h) una reacción inmunológica específica con un anticuerpo específicamente orientado contra la glicoproteína; caracterizado porque una solución de proteínas, en la que se puede reconocer inmunológicamente la glicoproteína, es sometida al menos a una de las siguientes medidas y se obtiene la fracción enriquecida respecto a la glicoproteína: a) adición de sales neutras hasta la separación de la glicoproteína y por precipitación; b) fraccionamiento en tamiz molecular y obtención de la fracción con un peso molecular de 25000 a 75000; c) adsorción de la glicoproteína en un cambiador de iones débilmente básico, y elución del mismo; d) adición de derivados solubles en agua de una base acridínica o quinoleínica, en el margen de pH 5-10, hasta una concentración final de aproximadamente 0,8%; e) tratamiento de la solución de glicoproteína con hidroxilapatito; f) electroforésis preparativa de zonas y obtención de la zona entre [alfa (sub 1)-globulinas y [alfa (sub 2)-globulinas; y g) tratamiento de la solución de proteínas con un inmuno-adsorbente.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19772726886 DE2726886A1 (de) | 1977-06-15 | 1977-06-15 | Neues glykoprotein und verfahren zu dessen herstellung |
Publications (1)
Publication Number | Publication Date |
---|---|
ES470653A1 true ES470653A1 (es) | 1979-09-01 |
Family
ID=6011534
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES470653A Expired ES470653A1 (es) | 1977-06-15 | 1978-06-09 | Procedimiento para el enriquecimiento de una glicoproteina |
Country Status (14)
Country | Link |
---|---|
US (1) | US4269825A (es) |
EP (1) | EP0000134B1 (es) |
JP (1) | JPS548717A (es) |
AT (1) | AT366063B (es) |
AU (1) | AU523785B2 (es) |
CA (1) | CA1110617A (es) |
DE (2) | DE2726886A1 (es) |
DK (1) | DK267278A (es) |
ES (1) | ES470653A1 (es) |
IE (1) | IE47092B1 (es) |
IT (1) | IT1113082B (es) |
MX (1) | MX5459E (es) |
NZ (1) | NZ187548A (es) |
ZA (1) | ZA783431B (es) |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2842467A1 (de) * | 1978-09-29 | 1980-04-10 | Behringwerke Ag | Neues ubiquitaeres gewebsprotein pp tief 8 |
DE2946458A1 (de) * | 1979-11-17 | 1981-06-11 | Behringwerke Ag, 3550 Marburg | Neues protein pp(pfeil abwaerts)11(pfeil abwaerts) |
DE2952792A1 (de) * | 1979-12-31 | 1981-07-02 | Behringwerke Ag, 3550 Marburg | Neues protein (pp(pfeil abwaerts)15(pfeil abwaerts)) mit immunsuppressiver wirkung |
DE3013724A1 (de) * | 1980-04-10 | 1981-10-15 | Behringwerke Ag, 3550 Marburg | Neues protein pp (pfeil abwaerts)9(pfeil abwaerts), verfahren zu seiner anreicherung und gewinnung sowieseine verwendung |
US4350687A (en) * | 1980-02-10 | 1982-09-21 | Research Corporation | Platelet derived cell growth factor |
JPS56145297A (en) * | 1980-04-11 | 1981-11-11 | Kureha Chem Ind Co Ltd | Preparative method of glycoprotein having immunosupressing activity |
DE3109629A1 (de) * | 1981-03-13 | 1982-09-23 | Behringwerke Ag, 3550 Marburg | "neues protein (pp(pfeil abwaerts)1(pfeil abwaerts)(pfeil abwaerts)6(pfeil abwaerts)), verfahren zu seiner anreicherung und gewinnung sowie seine verwendung" |
US4481137A (en) * | 1982-02-26 | 1984-11-06 | Mochida Pharmaceutical Co., Ltd. | Glycoproteins and processes for their production |
DE3315000A1 (de) * | 1983-04-26 | 1984-10-31 | Behringwerke Ag, 3550 Marburg | Gewebeprotein pp(pfeil abwaerts)4(pfeil abwaerts), verfahren zu seiner gewinnung sowie seine verwendung |
US4554256A (en) * | 1983-07-21 | 1985-11-19 | The Idaho Research Foundation, Inc. | Antigen associated with early detection of mammalian pregnancy |
US4524026A (en) * | 1983-08-29 | 1985-06-18 | Yoshio Sakagami | Novel proteinous cancer-cell proliferation inhibitory factors |
DE3334405A1 (de) * | 1983-09-23 | 1985-04-04 | Behringwerke Ag, 3550 Marburg | Membranassoziierte proteine (mp(pfeil abwaerts)2(pfeil abwaerts)), verfahren zu ihrer gewinnung sowie ihre verwendung |
JPS60199819A (ja) * | 1984-03-23 | 1985-10-09 | Kowa Co | トロンビン結合性物質およびその製法 |
JPS61294366A (ja) * | 1985-06-24 | 1986-12-25 | Toubishi Yakuhin Kogyo Kk | 糖鎖に特異的な抗体の検出用試薬及びその使用 |
DE3602688A1 (de) * | 1986-01-30 | 1987-08-06 | Behringwerke Ag | Verfahren zur gewinnung und herstellung eines pasteurisierten praeparates von (alpha)(pfeil abwaerts)2(pfeil abwaerts)-antiplasmin |
JPH0266456A (ja) * | 1988-08-31 | 1990-03-06 | Saikin Kagaku Kenkyusho:Kk | 疾病動物の一次診断方法、それに使用する診断試薬及びその製造方法 |
US5497003A (en) * | 1995-02-15 | 1996-03-05 | Servo Corporation Of America | Pyroelectric detector array with optical filter elements |
BG65084B1 (bg) * | 2002-03-13 | 2007-02-28 | Закрьiтое Акционерное Общество, Производственное Предприятие"Эндо-Фарм-А" | Нов клас биоактивни гликопротеини |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2221261A1 (de) * | 1972-04-29 | 1973-11-15 | Behringwerke Ag | Ap-glykoproteine und verfahren zu ihrer isolierung |
DE2256168A1 (de) * | 1972-11-16 | 1974-06-27 | Behringwerke Ag | Histidinreiches 3,8 s-alpha tief 2glykoprotein und verfahren zu seiner isolierung aus humanserum |
-
1977
- 1977-06-15 DE DE19772726886 patent/DE2726886A1/de not_active Withdrawn
-
1978
- 1978-06-09 ES ES470653A patent/ES470653A1/es not_active Expired
- 1978-06-09 DE DE7878100134T patent/DE2861256D1/de not_active Expired
- 1978-06-09 EP EP78100134A patent/EP0000134B1/de not_active Expired
- 1978-06-13 NZ NZ187548A patent/NZ187548A/en unknown
- 1978-06-13 IT IT24531/78A patent/IT1113082B/it active
- 1978-06-14 CA CA305,433A patent/CA1110617A/en not_active Expired
- 1978-06-14 ZA ZA00783431A patent/ZA783431B/xx unknown
- 1978-06-14 MX MX787147U patent/MX5459E/es unknown
- 1978-06-14 DK DK267278A patent/DK267278A/da not_active Application Discontinuation
- 1978-06-14 IE IE1198/78A patent/IE47092B1/en unknown
- 1978-06-14 AT AT0433878A patent/AT366063B/de not_active IP Right Cessation
- 1978-06-14 AU AU37086/78A patent/AU523785B2/en not_active Expired
- 1978-06-15 JP JP7157678A patent/JPS548717A/ja active Granted
-
1979
- 1979-12-19 US US06/105,416 patent/US4269825A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DK267278A (da) | 1978-12-16 |
IT1113082B (it) | 1986-01-20 |
IT7824531A0 (it) | 1978-06-13 |
AU3708678A (en) | 1979-12-20 |
US4269825A (en) | 1981-05-26 |
NZ187548A (en) | 1984-05-31 |
IE781198L (en) | 1978-12-15 |
CA1110617A (en) | 1981-10-13 |
EP0000134B1 (de) | 1981-10-28 |
JPS548717A (en) | 1979-01-23 |
EP0000134A1 (de) | 1979-01-10 |
MX5459E (es) | 1983-08-11 |
DE2726886A1 (de) | 1979-01-18 |
DE2861256D1 (en) | 1982-01-07 |
IE47092B1 (en) | 1983-12-14 |
AU523785B2 (en) | 1982-08-12 |
ATA433878A (de) | 1981-07-15 |
AT366063B (de) | 1982-03-10 |
JPS6361319B2 (es) | 1988-11-28 |
ZA783431B (en) | 1979-06-27 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD1A | Patent lapsed |
Effective date: 19980401 |