ES2521675T3 - Identificación y uso de [2Fe-2S]-dihidroxi-ácido deshidratasas bacterianas - Google Patents

Identificación y uso de [2Fe-2S]-dihidroxi-ácido deshidratasas bacterianas Download PDF

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ES2521675T3
ES2521675T3 ES09793126.5T ES09793126T ES2521675T3 ES 2521675 T3 ES2521675 T3 ES 2521675T3 ES 09793126 T ES09793126 T ES 09793126T ES 2521675 T3 ES2521675 T3 ES 2521675T3
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dhad
subset
sequences
seq
amino acid
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Dennis Flint
Steven Cary Rothman
Wonchul Suh
Jean-Francois Tomb
Rick W. Ye
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Butamax Advanced Biofuels LLC
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Butamax Advanced Biofuels LLC
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    • C12N9/88—Lyases (4.)
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    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
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    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
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    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01009—Dihydroxy-acid dehydratase (4.2.1.9), i.e. acetohydroxyacid dehydratase
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    • G16B30/10—Sequence alignment; Homology search
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    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un método de identificación de enzimas [2Fe-2S] DHAD que comprende: a) consultar una o más secuencias de aminoácidos con un perfil modelo oculto de Markov preparado usando las proteínas de SEC ID Nº: 164, 168, 230, 232, 298, 310, 344 y 346, en el que una coincidencia con un valor de E inferior a 10-5 proporciona un primer subconjunto de secuencias, por el cual dicho primer subconjunto de secuencias se corresponde con una o más proteínas relacionadas con DHAD; b) analizar el primer subconjunto de secuencias que se corresponde con una o más proteínas relacionadas con DHAD de la etapa (a) para la presencia de tres cisteínas conservadas que se corresponden con las posiciones 56, 129 y 201 en la secuencia de aminoácidos de dihidroxi-ácido deshidratasa de Streptococcus mutans (SEC ID Nº: 168), por el cual se identifican un segundo subconjunto de secuencias que codifican enzimas [2Fe-2S] DHAD; y c) analizar el segundo subconjunto de secuencias de la etapa (b) para la presencia de aminoácidos conservados distintivos en las posiciones correspondientes a las posiciones en la secuencia de aminoácidos de DHAD de Streptococcus mutans (SEC ID Nº: 168) que son ácido aspártico en la posición 88, arginina o asparagina en la posición 142, asparagina en la posición 208 y leucina en la posición 454, por el cual se identifican adicionalmente un tercer subconjunto de secuencias que codifica enzimas [2Fe-2S] DHAD.
ES09793126.5T 2008-09-29 2009-09-29 Identificación y uso de [2Fe-2S]-dihidroxi-ácido deshidratasas bacterianas Active ES2521675T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10079208P 2008-09-29 2008-09-29
US100792P 2008-09-29
PCT/US2009/058827 WO2010037112A1 (en) 2008-09-29 2009-09-29 IDENTIFICATION AND USE OF BACTERIAL [2Fe-2S] DIHYDROXY-ACID DEHYDRATASES

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ES2521675T3 true ES2521675T3 (es) 2014-11-13

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US (3) US20100081154A1 (es)
EP (2) EP2337851B1 (es)
JP (1) JP5846912B2 (es)
KR (1) KR101659101B1 (es)
CN (1) CN102186973B (es)
AU (1) AU2009296225B2 (es)
BR (1) BRPI0913682A2 (es)
CA (1) CA2735690A1 (es)
ES (1) ES2521675T3 (es)
MX (1) MX2011003238A (es)
NZ (1) NZ591244A (es)
WO (1) WO2010037112A1 (es)
ZA (2) ZA201101367B (es)

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