ES2521675T3 - Identificación y uso de [2Fe-2S]-dihidroxi-ácido deshidratasas bacterianas - Google Patents
Identificación y uso de [2Fe-2S]-dihidroxi-ácido deshidratasas bacterianas Download PDFInfo
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- ES2521675T3 ES2521675T3 ES09793126.5T ES09793126T ES2521675T3 ES 2521675 T3 ES2521675 T3 ES 2521675T3 ES 09793126 T ES09793126 T ES 09793126T ES 2521675 T3 ES2521675 T3 ES 2521675T3
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- G—PHYSICS
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- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
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- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
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- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/16—Butanols
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/01—Hydro-lyases (4.2.1)
- C12Y402/01009—Dihydroxy-acid dehydratase (4.2.1.9), i.e. acetohydroxyacid dehydratase
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
- G16B30/10—Sequence alignment; Homology search
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Un método de identificación de enzimas [2Fe-2S] DHAD que comprende: a) consultar una o más secuencias de aminoácidos con un perfil modelo oculto de Markov preparado usando las proteínas de SEC ID Nº: 164, 168, 230, 232, 298, 310, 344 y 346, en el que una coincidencia con un valor de E inferior a 10-5 proporciona un primer subconjunto de secuencias, por el cual dicho primer subconjunto de secuencias se corresponde con una o más proteínas relacionadas con DHAD; b) analizar el primer subconjunto de secuencias que se corresponde con una o más proteínas relacionadas con DHAD de la etapa (a) para la presencia de tres cisteínas conservadas que se corresponden con las posiciones 56, 129 y 201 en la secuencia de aminoácidos de dihidroxi-ácido deshidratasa de Streptococcus mutans (SEC ID Nº: 168), por el cual se identifican un segundo subconjunto de secuencias que codifican enzimas [2Fe-2S] DHAD; y c) analizar el segundo subconjunto de secuencias de la etapa (b) para la presencia de aminoácidos conservados distintivos en las posiciones correspondientes a las posiciones en la secuencia de aminoácidos de DHAD de Streptococcus mutans (SEC ID Nº: 168) que son ácido aspártico en la posición 88, arginina o asparagina en la posición 142, asparagina en la posición 208 y leucina en la posición 454, por el cual se identifican adicionalmente un tercer subconjunto de secuencias que codifica enzimas [2Fe-2S] DHAD.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10079208P | 2008-09-29 | 2008-09-29 | |
US100792P | 2008-09-29 | ||
PCT/US2009/058827 WO2010037112A1 (en) | 2008-09-29 | 2009-09-29 | IDENTIFICATION AND USE OF BACTERIAL [2Fe-2S] DIHYDROXY-ACID DEHYDRATASES |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2521675T3 true ES2521675T3 (es) | 2014-11-13 |
Family
ID=41402186
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES09793126.5T Active ES2521675T3 (es) | 2008-09-29 | 2009-09-29 | Identificación y uso de [2Fe-2S]-dihidroxi-ácido deshidratasas bacterianas |
Country Status (13)
Country | Link |
---|---|
US (3) | US20100081154A1 (es) |
EP (2) | EP2337851B1 (es) |
JP (1) | JP5846912B2 (es) |
KR (1) | KR101659101B1 (es) |
CN (1) | CN102186973B (es) |
AU (1) | AU2009296225B2 (es) |
BR (1) | BRPI0913682A2 (es) |
CA (1) | CA2735690A1 (es) |
ES (1) | ES2521675T3 (es) |
MX (1) | MX2011003238A (es) |
NZ (1) | NZ591244A (es) |
WO (1) | WO2010037112A1 (es) |
ZA (2) | ZA201101367B (es) |
Families Citing this family (65)
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US8188250B2 (en) | 2008-04-28 | 2012-05-29 | Butamax(Tm) Advanced Biofuels Llc | Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans |
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WO2010037105A1 (en) * | 2008-09-29 | 2010-04-01 | Butamax™ Advanced Biofuels LLC | Enhanced dihydroxy-acid dehydratase activity in lactic acid bacteria |
US20160222370A1 (en) * | 2008-09-29 | 2016-08-04 | Butamax Advanced Biofuels Llc | Recombinant Yeast Host Cell With Fe-S Cluster Proteins And Methods Of Using Thereof |
AU2009296225B2 (en) | 2008-09-29 | 2015-09-17 | Butamax(Tm) Advanced Biofuels Llc | Identification and use of bacterial [2Fe-2S] dihydroxy-acid dehydratases |
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US8465964B2 (en) * | 2008-11-13 | 2013-06-18 | Butamax (TM) Advanced Biofules LLC | Increased production of isobutanol in yeast with reduced mitochondrial amino acid biosynthesis |
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-
2009
- 2009-09-29 AU AU2009296225A patent/AU2009296225B2/en active Active
- 2009-09-29 KR KR1020117009692A patent/KR101659101B1/ko active IP Right Grant
- 2009-09-29 JP JP2011529364A patent/JP5846912B2/ja active Active
- 2009-09-29 WO PCT/US2009/058827 patent/WO2010037112A1/en active Application Filing
- 2009-09-29 EP EP09793126.5A patent/EP2337851B1/en active Active
- 2009-09-29 ES ES09793126.5T patent/ES2521675T3/es active Active
- 2009-09-29 EP EP14180626.5A patent/EP2821484B1/en active Active
- 2009-09-29 CA CA2735690A patent/CA2735690A1/en not_active Abandoned
- 2009-09-29 BR BRPI0913682-7A patent/BRPI0913682A2/pt not_active Application Discontinuation
- 2009-09-29 NZ NZ591244A patent/NZ591244A/xx unknown
- 2009-09-29 US US12/569,636 patent/US20100081154A1/en not_active Abandoned
- 2009-09-29 CN CN200980138542.2A patent/CN102186973B/zh active Active
- 2009-09-29 MX MX2011003238A patent/MX2011003238A/es not_active Application Discontinuation
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2011
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2013
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- 2013-03-15 US US13/838,508 patent/US8951937B2/en active Active
- 2013-12-20 ZA ZA2013/09658A patent/ZA201309658B/en unknown
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BRPI0913682A2 (pt) | 2020-08-11 |
WO2010037112A1 (en) | 2010-04-01 |
CN102186973A (zh) | 2011-09-14 |
NZ591244A (en) | 2013-03-28 |
EP2337851B1 (en) | 2014-08-13 |
EP2337851A1 (en) | 2011-06-29 |
CN102186973B (zh) | 2017-08-15 |
AU2009296225B2 (en) | 2015-09-17 |
KR20110063575A (ko) | 2011-06-10 |
EP2821484B1 (en) | 2016-06-29 |
US20140051137A1 (en) | 2014-02-20 |
ZA201309658B (en) | 2016-06-29 |
KR101659101B1 (ko) | 2016-09-22 |
CA2735690A1 (en) | 2010-04-01 |
ZA201101367B (en) | 2014-03-26 |
US20100081154A1 (en) | 2010-04-01 |
US8951937B2 (en) | 2015-02-10 |
US20140030776A1 (en) | 2014-01-30 |
JP5846912B2 (ja) | 2016-01-20 |
AU2009296225A1 (en) | 2010-04-01 |
JP2012503993A (ja) | 2012-02-16 |
EP2821484A1 (en) | 2015-01-07 |
MX2011003238A (es) | 2011-04-28 |
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