BR112012020589B8 - célula hospedeira recombinante de levedura, método para fabricar um produto, método para converter 2,3-diidroxiisovalerato em alfa-cetoisovalerato, método para aumentar a atividade específica de um polipeptídeo heterólogo, método para aumentar o fluxo em uma via biossintética e método para aumentar a atividade de uma proteína - Google Patents

célula hospedeira recombinante de levedura, método para fabricar um produto, método para converter 2,3-diidroxiisovalerato em alfa-cetoisovalerato, método para aumentar a atividade específica de um polipeptídeo heterólogo, método para aumentar o fluxo em uma via biossintética e método para aumentar a atividade de uma proteína

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BR112012020589B8
BR112012020589B8 BR112012020589A BR112012020589A BR112012020589B8 BR 112012020589 B8 BR112012020589 B8 BR 112012020589B8 BR 112012020589 A BR112012020589 A BR 112012020589A BR 112012020589 A BR112012020589 A BR 112012020589A BR 112012020589 B8 BR112012020589 B8 BR 112012020589B8
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increasing
activity
host cell
flux
making
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BR112012020589A
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BR112012020589B1 (pt
BR112012020589A2 (pt
BR112012020589A8 (pt
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James Paul Brian
Flint Dennis
W Ye Rick
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Butamax Tm Advanced Biofuels
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
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Abstract

células hospedeiras recombinantes, métodos para fabricar um produto e para fabricar isobutanol, método para converter 2,3-diidroxiisovalerato em a-cetoisovalerato, métodos para aumentar a atividade específica de um polipeptídeo heterólogo, o fluxo em uma via blosslntética e a atividade de uma proteína, métodos para identificar polipeptídeos e método para medir a concentração de formas de polipeptídeo. a presente invenção está relacionada a uma célula hospedeira recombinante, em particular uma célula de levedura, que compreende um polipeptídeo de diidroxiácido desidratase. a invenção está também relacionada a uma célula hospedeira recombinante que tem atividade específica aumentada do polipeptídeo de diidroxiácido desidratase como um resultado de expressão aumentada do polipeptídeo, modulação da biosslntese de cluster de fe-s da célula ou uma combinação dos mesmos. a presente invenção também inclui métodos de uso das células hospedeiras, bem como, métodos para identificar polipeptídeos que aumentam o fluxo em uma via biossintética de cluster de fe-s em uma célula hospedeira.
BR112012020589A 2010-02-17 2011-02-17 célula hospedeira recombinante de levedura, método para fabricar um produto, método para converter 2,3-diidroxiisovalerato em alfa-cetoisovalerato, método para aumentar a atividade específica de um polipeptídeo heterólogo, método para aumentar o fluxo em uma via biossintética e método para aumentar a atividade de uma proteína BR112012020589B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US30533310P 2010-02-17 2010-02-17
US61/305,333 2010-02-17
PCT/US2011/025258 WO2011103300A2 (en) 2010-02-17 2011-02-17 Improving activity of fe-s cluster requiring proteins

Publications (4)

Publication Number Publication Date
BR112012020589A2 BR112012020589A2 (pt) 2015-11-03
BR112012020589A8 BR112012020589A8 (pt) 2020-12-15
BR112012020589B1 BR112012020589B1 (pt) 2020-12-29
BR112012020589B8 true BR112012020589B8 (pt) 2021-05-18

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BR112012020589A BR112012020589B8 (pt) 2010-02-17 2011-02-17 célula hospedeira recombinante de levedura, método para fabricar um produto, método para converter 2,3-diidroxiisovalerato em alfa-cetoisovalerato, método para aumentar a atividade específica de um polipeptídeo heterólogo, método para aumentar o fluxo em uma via biossintética e método para aumentar a atividade de uma proteína

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US (4) US9297016B2 (pt)
EP (1) EP2536821B1 (pt)
JP (2) JP5950830B2 (pt)
KR (1) KR20130027063A (pt)
CN (1) CN102782119B (pt)
AU (1) AU2011218090B2 (pt)
BR (1) BR112012020589B8 (pt)
CA (1) CA2788842C (pt)
MX (2) MX357290B (pt)
NZ (1) NZ601241A (pt)
WO (1) WO2011103300A2 (pt)
ZA (1) ZA201205427B (pt)

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US9611482B2 (en) 2017-04-04
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AU2011218090A1 (en) 2012-08-02
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BR112012020589B1 (pt) 2020-12-29
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