ES2269020T1 - Variantes de amilasa. - Google Patents
Variantes de amilasa. Download PDFInfo
- Publication number
- ES2269020T1 ES2269020T1 ES05002888T ES05002888T ES2269020T1 ES 2269020 T1 ES2269020 T1 ES 2269020T1 ES 05002888 T ES05002888 T ES 05002888T ES 05002888 T ES05002888 T ES 05002888T ES 2269020 T1 ES2269020 T1 ES 2269020T1
- Authority
- ES
- Spain
- Prior art keywords
- variant
- amylase
- bacillus
- amino acid
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C5/00—Other raw materials for the preparation of beer
- C12C5/004—Enzymes
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38672—Granulated or coated enzymes
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38681—Chemically modified or immobilised enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06L—DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS, YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS; BLEACHING LEATHER OR FURS
- D06L1/00—Dry-cleaning or washing fibres, filaments, threads, yarns, fabrics, feathers or made-up fibrous goods
- D06L1/12—Dry-cleaning or washing fibres, filaments, threads, yarns, fabrics, feathers or made-up fibrous goods using aqueous solvents
- D06L1/14—De-sizing
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
- D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
- D21C5/02—Working-up waste paper
- D21C5/022—Chemicals therefor
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
- D21H17/005—Microorganisms or enzymes
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
- D21H17/20—Macromolecular organic compounds
- D21H17/21—Macromolecular organic compounds of natural origin; Derivatives thereof
- D21H17/24—Polysaccharides
- D21H17/28—Starch
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
- D21C5/02—Working-up waste paper
- D21C5/025—De-inking
- D21C5/027—Chemicals therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/64—Paper recycling
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Textile Engineering (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- General Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Variante de una a-amilasa madre, que presenta al menos un 80% de identidad con la secuencia de aminoácidos SEC ID N°. 1, en la que la variante comprende una deleción de aminoácidos equivalente a R179 y G180 de la secuencia de aminoácidos mostrada en SEC ID N°3, dicha variante teniendo actividad alfa-amilasa y exhibiendo al menos una de las siguientes propiedades con respecto a dicha alfa-amilasa madre:termostabilidad aumentada; estabilidad aumentada a la oxidación; y dependencia de Ca2+ reducida.
Claims (23)
1. Variante de una
\alpha-amilasa madre, que presenta al menos un
80% de identidad con la secuencia de aminoácidos SEC ID N°. 1, en
la que la variante comprende una deleción de aminoácidos
equivalente a R179 y G180 de la secuencia de aminoácidos mostrada en
SEC ID N°3, dicha variante teniendo actividad
\alpha-amilasa y exhibiendo al menos una de las
siguientes propiedades con respecto a dicha
\alpha-amilasa madre: termostabilidad aumentada;
estabilidad aumentada a la oxidación; y dependencia de Ca^{2+}
reducida.
2. Variante según la reivindicación 1, donde al
menos un residuo de aminoácido oxidable de dicha
\alpha-amilasa madre ha sido delecionado o ha
sido sustituido por un residuo de aminoácido diferente que es menos
susceptible a la oxidación que dicho residuo de aminoácido
oxidable.
3. Variante según la reivindicación 2, donde
dicho residuo de aminoácido oxidable es seleccionado del grupo
formado por metionina, triptófano, cisteina y tirosina.
4. Variante según la reivindicación 3, en la
que el residuo de metionina ha sido reemplazado por treonina.
5. Constructo de ADN que comprende una
secuencia de ADN que codifica una variante de la
\alpha-amilasa según cualquiera de las
reivindicaciones
1-4.
1-4.
6. Vector de expresión recombinante que lleva
un constructo de ADN según la reivindicación 5.
7. Célula huésped que es transformada con un
constructo de ADN según la reivindicación 5 o un vector según la
reivindicación 6.
8. Célula según la reivindicación 7, que es un
microorganismo.
9. Célula según la reivindicación 8, que es una
bacteria o un hongo.
10. Célula según la reivindicación 8, que es
una bacteria gram positiva tal como Bacillus subtilis, Bacillus
licheniformis, Bacillus lentus, Bacillus brevis, Bacillus
stearothermophilus, Bacillus alkalophilus, Bacillus
amyloliquefaciens, Bacillus coagulans, Bacillus circulans, Bacillus
lautus, Bacillus thuringiensis o Streptomyces lividans o
Streptomyces murinus, o una bacteria
gram-negativa tal como E. coli.
11. Método para producir una variante de
\alpha-amilasa según cualquiera de las
reivindicaciones 1-4, donde una célula según
cualquiera de las reivindicaciones
7-10 es cultivada bajo condiciones propicias para la producción de la variante de \alpha-amilasa, y la variante de \alpha-amilasa es posteriormente recuperada del cultivo.
7-10 es cultivada bajo condiciones propicias para la producción de la variante de \alpha-amilasa, y la variante de \alpha-amilasa es posteriormente recuperada del cultivo.
12. Uso de una variante de
\alpha-amilasa según cualquiera de las
reivindicaciones 1-4 para el lavado de la ropa y/o
de la vajilla.
13. Aditivo de detergente que comprende una
variante de la \alpha-amilasa según cualquiera de
las reivindicaciones 1-4, opcionalmente en forma de
un granulado no pulverulento, líquido estabilizado o enzima
protegida.
14. Aditivo de detergente según la
reivindicación 13, que comprende 0,02-200 mg de
proteína enzimática por gramo de aditivo.
15. Aditivo de detergente según la
reivindicación 13 o 14, que adicionalmente comprende otra enzima
tal como una proteasa, una lipasa, una peroxidasa, otra enzima
amilolítica y/o una celulasa.
16. Composición de detergente que comprende una
variante de la \alpha-amilasa según cualquiera de
las reivindicaciones 1-4, y un tensioactivo.
17. Composición de detergente según la
reivindicación 16, que adicionalmente comprende otra enzima tal
como una proteasa, una lipasa, una peroxidasa, otra enzima
amilolítica y/o una celulasa.
18. Composición de detergente para el lavado
manual o automático de la vajilla que comprende una variante de la
\alpha-amilasa según cualquiera de las
reivindicaciones 1-4, y un tensioactivo.
19. Composición de detergente para el lavado de
la vajilla según la reivindicación 18, que adicionalmente comprende
otra enzima tal como una proteasa, una lipasa, una peroxidasa, otra
enzima amilolítica y/o una celulasa.
20. Composición para el lavado manual o
automático de la ropa que comprende una variante de la
\alpha-amilasa según cualquiera de las
reivindicaciones 1-4, y un tensioactivo.
21. Composición para el lavado de la ropa según
la reivindicación 20, que adicionalmente comprende otra enzima tal
como una proteasa, una lipasa, una peroxidasa, una enzima
amilolítica y/o una celulasa.
22. Uso de una variante de
\alpha-amilasa según cualquiera de las
reivindicaciones 1-4 para el desencolado textil.
23. Uso de una variante de
\alpha-amilasa según cualquiera de las
reivindicaciones 1-4 para la producción de
suavizantes y etanol a partir de almidón.
Applications Claiming Priority (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK12695 | 1995-02-03 | ||
DK126/95 | 1995-02-03 | ||
DK33695 | 1995-03-29 | ||
DK336/95 | 1995-03-29 | ||
DK109795 | 1995-09-29 | ||
DK1097/95 | 1995-09-29 | ||
DK1121/95 | 1995-10-06 | ||
DK112195 | 1995-10-06 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2269020T1 true ES2269020T1 (es) | 2007-04-01 |
ES2269020T3 ES2269020T3 (es) | 2007-10-01 |
Family
ID=27439257
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES96900894T Expired - Lifetime ES2226609T3 (es) | 1995-02-03 | 1996-02-05 | Variantes de amilasa. |
ES05002888T Expired - Lifetime ES2269020T3 (es) | 1995-02-03 | 1996-02-05 | Variantes dee amilasa. |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES96900894T Expired - Lifetime ES2226609T3 (es) | 1995-02-03 | 1996-02-05 | Variantes de amilasa. |
Country Status (14)
Country | Link |
---|---|
EP (6) | EP2455468A3 (es) |
JP (2) | JP4116074B2 (es) |
CN (2) | CN100535109C (es) |
AR (3) | AR000862A1 (es) |
AT (2) | ATE335077T1 (es) |
AU (1) | AU4483396A (es) |
BR (1) | BR9607735A (es) |
CA (2) | CA2211405C (es) |
DE (4) | DE69637036T2 (es) |
DK (2) | DK1538155T3 (es) |
ES (2) | ES2226609T3 (es) |
MX (1) | MX9705727A (es) |
PT (1) | PT1538155E (es) |
WO (1) | WO1996023873A1 (es) |
Families Citing this family (837)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6440716B1 (en) | 1995-02-03 | 2002-08-27 | Novozymes A/S | α-amylase mutants |
CN100419076C (zh) | 1995-02-03 | 2008-09-17 | 诺沃奇梅兹有限公司 | 设计具有预定特性的α-淀粉酶突变体的方法 |
ES2231859T3 (es) * | 1996-03-07 | 2005-05-16 | THE PROCTER & GAMBLE COMPANY | Composiciones detergentes que comprenden proteasas y amilasas mejoradas. |
US5763385A (en) * | 1996-05-14 | 1998-06-09 | Genencor International, Inc. | Modified α-amylases having altered calcium binding properties |
BR9710440A (pt) * | 1996-05-15 | 1999-08-17 | Procter & Gamble | Composi-{es de detergente compreendendo amilases aperfei-oadas celulase e tensoativo catiÄnico |
US6197070B1 (en) | 1996-05-15 | 2001-03-06 | The Procter & Gamble Company | Detergent compositions comprising alpha combination of α-amylases for malodor stripping |
US6696251B1 (en) | 1996-05-31 | 2004-02-24 | Board Of Trustees Of The University Of Illinois | Yeast cell surface display of proteins and uses thereof |
HUP9903559A3 (en) * | 1996-08-01 | 2000-08-28 | Procter & Gamble | Detergent compositions comprising improved amylase for dingy fabric clean-up |
GB9701425D0 (en) | 1997-01-24 | 1997-03-12 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
US6486113B1 (en) | 1997-03-31 | 2002-11-26 | Kao Corporation | Mutant α-amylases |
EP0994936A1 (en) * | 1997-07-02 | 2000-04-26 | The Procter & Gamble Company | Dishwashing compositions comprising a phospholipase and an amylase |
JP4358431B2 (ja) * | 1997-10-13 | 2009-11-04 | ノボザイムス アクティーゼルスカブ | α−アミラーゼ変異体 |
US6361989B1 (en) | 1997-10-13 | 2002-03-26 | Novozymes A/S | α-amylase and α-amylase variants |
MA25044A1 (fr) * | 1997-10-23 | 2000-10-01 | Procter & Gamble | Compositions de lavage contenant des variants de proteases multisubstituees. |
CN1163597C (zh) * | 1997-10-30 | 2004-08-25 | 诺维信公司 | α-淀粉酶突变体 |
KR100770721B1 (ko) * | 1997-10-30 | 2007-10-29 | 노보자임스 에이/에스 | α-아밀라제 돌연변이체 |
US7078213B1 (en) | 1998-02-18 | 2006-07-18 | Novozymes A/S | Alkaline Bacillus amylase |
BRPI9911086B1 (pt) | 1998-06-10 | 2016-08-02 | Novozymes As | composição de limpeza, processo para tratar tecidos a máquina, e uso de uma mananase |
US6887986B1 (en) | 1998-11-16 | 2005-05-03 | Novozymes A/S | α-amylase variants |
US6197565B1 (en) * | 1998-11-16 | 2001-03-06 | Novo-Nordisk A/S | α-Amylase variants |
CN1333748A (zh) | 1998-11-20 | 2002-01-30 | 宝洁公司 | 漂白活性剂的改进合成方法 |
EP1803817B1 (en) | 1998-12-18 | 2011-04-06 | Novozymes A/S | Subtilase enzymes of the I-S1 and I-S2 sub-groups having an additional amino acid residue in an active site loop region |
AU2495900A (en) * | 1999-01-11 | 2000-08-01 | Procter & Gamble Company, The | Cleaning compositions containing a multi-function component and method for using |
US6410295B1 (en) | 1999-03-30 | 2002-06-25 | Novozymes A/S | Alpha-amylase variants |
CA2365438C (en) | 1999-03-30 | 2015-07-14 | Novozymes A/S | Alpha-amylase variants |
US6623948B1 (en) | 1999-03-31 | 2003-09-23 | Novozymes A/S | Nucleic acid sequences encoding alkaline alpha-amylases |
CN100523181C (zh) | 1999-03-31 | 2009-08-05 | 诺维信公司 | 具有碱性α-淀粉酶活性的多肽及其编码核酸 |
US6309871B1 (en) * | 1999-03-31 | 2001-10-30 | Novozymes A/S | Polypeptides having alkaline α-amylase activity |
EP2889375B1 (en) * | 1999-03-31 | 2019-03-20 | Novozymes A/S | Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same |
AU4392900A (en) | 1999-05-20 | 2000-12-12 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 127 and 128 |
AU4393100A (en) | 1999-05-20 | 2000-12-12 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 125 and 126 |
ATE408676T1 (de) | 1999-05-20 | 2008-10-15 | Novozymes As | Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 132 und 133 |
EP1183340B1 (en) | 1999-05-20 | 2008-09-03 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 126 and 127 |
ATE408680T1 (de) | 1999-05-20 | 2008-10-15 | Novozymes As | Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 128 und 129 |
WO2000071687A1 (en) | 1999-05-20 | 2000-11-30 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 129 and 130 |
WO2001016285A2 (en) | 1999-08-31 | 2001-03-08 | Novozymes A/S | Novel proteases and variants thereof |
US7005288B1 (en) | 1999-11-10 | 2006-02-28 | Novozymes A/S | Fungamyl-like alpha-amylase variants |
EP1980614A3 (en) | 1999-11-10 | 2009-04-08 | Novozymes A/S | Fungamyl-like Alpha-Amylase Variants |
WO2001064852A1 (en) * | 2000-03-03 | 2001-09-07 | Novozymes A/S | Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same |
US7319112B2 (en) | 2000-07-14 | 2008-01-15 | The Procter & Gamble Co. | Non-halogenated antibacterial agents and processes for making same |
PL366249A1 (en) | 2000-07-28 | 2005-01-24 | Henkel Kommanditgesellschaft Auf Aktien | Novel amylolytic enzyme extracted from bacillus sp. a 7-7 (dsm 12368) and washing and cleaning agents containing this novel amylolytic enzyme |
EP2204446A1 (en) * | 2000-08-01 | 2010-07-07 | Novozymes A/S | Alpha-amylase mutants with altered properties |
EP1313846B1 (en) | 2000-08-21 | 2013-10-16 | Novozymes A/S | Subtilase enzymes |
GB2366797A (en) | 2000-09-13 | 2002-03-20 | Procter & Gamble | Process for making foam component by pressurising/depressurising |
JP4426716B2 (ja) | 2000-10-11 | 2010-03-03 | 花王株式会社 | 高生産性α−アミラーゼ |
EP1975229A3 (en) | 2000-10-13 | 2009-03-18 | Novozymes A/S | Alpha-amylase variant with altered properties |
WO2002031133A1 (en) | 2000-10-13 | 2002-04-18 | Novozymes A/S | Subtilase variants |
EP1326965A2 (en) * | 2000-10-13 | 2003-07-16 | Novozymes A/S | Alpha-amylase variant with altered properties |
ATE400639T1 (de) | 2000-10-27 | 2008-07-15 | Procter & Gamble | Stabilisierte flüssige zusammensetzungen |
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- 1996-02-05 DE DE05002888T patent/DE05002888T1/de active Pending
- 1996-02-05 ES ES05002888T patent/ES2269020T3/es not_active Expired - Lifetime
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- 1996-02-05 AT AT96900894T patent/ATE335077T1/de active
- 1996-02-05 CA CA 2211405 patent/CA2211405C/en not_active Expired - Lifetime
- 1996-02-05 BR BR9607735A patent/BR9607735A/pt not_active Application Discontinuation
- 1996-02-05 PT PT05002888T patent/PT1538155E/pt unknown
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- 1996-02-05 DE DE1996636408 patent/DE69636408T2/de not_active Expired - Lifetime
- 1996-02-05 EP EP20060117580 patent/EP1752525A1/en not_active Withdrawn
- 1996-02-05 AU AU44833/96A patent/AU4483396A/en not_active Abandoned
- 1996-02-05 MX MX9705727A patent/MX9705727A/es unknown
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- 1996-02-05 DK DK05002888T patent/DK1538155T3/da active
- 1996-02-05 DK DK96900894T patent/DK0815208T3/da active
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