ES2141088T3 - Mejoras en la especificidad y conveniencia de la reaccion en cadena de la polimerasa. - Google Patents

Mejoras en la especificidad y conveniencia de la reaccion en cadena de la polimerasa.

Info

Publication number
ES2141088T3
ES2141088T3 ES91904707T ES91904707T ES2141088T3 ES 2141088 T3 ES2141088 T3 ES 2141088T3 ES 91904707 T ES91904707 T ES 91904707T ES 91904707 T ES91904707 T ES 91904707T ES 2141088 T3 ES2141088 T3 ES 2141088T3
Authority
ES
Spain
Prior art keywords
pcr
reaction
enzymatic
polymerase chain
pcr reagents
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91904707T
Other languages
English (en)
Inventor
Will Bloch
Jonathan Raymond
Alan R Read
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of ES2141088T3 publication Critical patent/ES2141088T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C59/00—Surface shaping of articles, e.g. embossing; Apparatus therefor
    • B29C59/10—Surface shaping of articles, e.g. embossing; Apparatus therefor by electric discharge treatment
    • B29C59/103—Surface shaping of articles, e.g. embossing; Apparatus therefor by electric discharge treatment of profiled articles, e.g. hollow or tubular articles
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J7/00—Chemical treatment or coating of shaped articles made of macromolecular substances
    • C08J7/04—Coating
    • C08J7/0427—Coating with only one layer of a composition containing a polymer binder
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J7/00—Chemical treatment or coating of shaped articles made of macromolecular substances
    • C08J7/04—Coating
    • C08J7/046—Forming abrasion-resistant coatings; Forming surface-hardening coatings
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2471/00—Characterised by the use of polyethers obtained by reactions forming an ether link in the main chain; Derivatives of such polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178—Special arrangements of analysers
    • G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
    • G01N2035/00247—Microvalves
    • G01N2035/00267—Meltable plugs
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178—Special arrangements of analysers
    • G01N2035/00277—Special precautions to avoid contamination (e.g. enclosures, glove- boxes, sealed sample carriers, disposal of contaminated material)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

MEJORAS EN LA REACCION DE LA CADENA DE POLIMERASA (RCP), UN PROCESO PARA LA AMPLIFICACION ENZIMATICA IN VITRO DE SECUENCIAS DE ACIDO NUCLEICO ESPECIFICAS, PUEDE REALIZARSE CAMBIANDO LA FORMA EN QUE ESTAN MEZCLADOS LOS REACTIVOS DE RCP Y EN LA QUE SE INICIA LA REACCION ENZIMATICA Y MEDIANTE EL CAMBIO DE UN ACEITE MINERAL, COMUNMENTE UTILIZADO COMO UNA BARRERA DE VAPOR PARA REDUCIR LA EVAPORACION DE DISOLVENTE, MEDIANTE UNA GRASA O CERA. LA UTILIZACION DE TALES MEZCLAS PERMITE LA DEMORA DE MEZCLADO DEL REACTIVO HASTA LA PRIMERA FASE DE CALENTAMIENTO DE UNA AMPLIFICACION DEL RCP, REDUCIENDO POR TANTO LA GENERACION ENZIMATICA DE PRODUCTOS NO ESPECIFICOS QUE SE PRODUCEN CUANDO UNA MEZCLA COMPLETA DE REACTIVOS DEL RCP, CON O SIN MUESTRA DE PRUEBA, PERMANECEN A UNA TEMPERATURA AMBIENTE O INFERIOR. ESTAS MEZCLAS AUMENTAN LA DURACION DE REACTIVOS DE RCP Y AUMENTAN LA PROTECCION DEL ENTORNO DE LABORATORIO CONTRA LA CONTAMINACION POR EL PRODUCTO DE RCP.
ES91904707T 1990-02-16 1991-02-15 Mejoras en la especificidad y conveniencia de la reaccion en cadena de la polimerasa. Expired - Lifetime ES2141088T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US48150190A 1990-02-16 1990-02-16

Publications (1)

Publication Number Publication Date
ES2141088T3 true ES2141088T3 (es) 2000-03-16

Family

ID=23912174

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91904707T Expired - Lifetime ES2141088T3 (es) 1990-02-16 1991-02-15 Mejoras en la especificidad y conveniencia de la reaccion en cadena de la polimerasa.

Country Status (10)

Country Link
US (1) US5411876A (es)
EP (1) EP0515506B1 (es)
JP (2) JP3087907B2 (es)
AT (1) ATE188509T1 (es)
AU (1) AU653712B2 (es)
CA (1) CA2075050C (es)
DE (1) DE69131891T2 (es)
DK (1) DK0515506T3 (es)
ES (1) ES2141088T3 (es)
WO (1) WO1991012342A1 (es)

Families Citing this family (172)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5674679A (en) * 1991-09-27 1997-10-07 Amersham Life Science, Inc. DNA cycle sequencing
WO1993015222A1 (fr) * 1992-01-29 1993-08-05 Institut National De La Sante Et De La Recherche Medicale (Inserm) Procede pour eviter les risques de contamination, notamment applicable aux techniques d'amplification de l'adn ou de l'arn
US5413924A (en) * 1992-02-13 1995-05-09 Kosak; Kenneth M. Preparation of wax beads containing a reagent for release by heating
EP0572057A1 (en) * 1992-05-11 1993-12-01 Johnson & Johnson Clinical Diagnostics, Inc. PCR reagent composition, test kit and methods for amplification and detection with reduced nonspecific amplification of nucleic acids
GB9211979D0 (en) * 1992-06-05 1992-07-15 Buchard Ole Uses of nucleic acid analogues
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5599660A (en) * 1993-01-19 1997-02-04 Pharmacia Biotech Inc. Method and preparation for sequential delivery of wax-embedded, inactivated biological and chemical reagents
AU6029694A (en) * 1993-01-19 1994-08-15 Pharmacia P-L Biochemicals, Inc. Storage and delivery of purified protein reagents with carrier wax
WO1995011315A1 (en) * 1993-10-22 1995-04-27 Invitrogen Corporation Packaging of reaction components in wax for release under specified temperature conditions
EP0724483A1 (en) * 1993-10-22 1996-08-07 Abbott Laboratories Reaction tube and method of use to minimize contamination
AU2871095A (en) * 1994-06-24 1996-01-19 University Of Houston, The Encapsulated pcr reagents
ZA956776B (en) * 1994-08-25 1996-03-20 Akzo Nobel Nv A process for making the product of a nucleic acid amplification reaction incapable of being a target for further amplification a diagnostic assay employing said process a kit and a container suitable for carrying out said process for assay
US5622822A (en) 1994-09-13 1997-04-22 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same
US5582988A (en) 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5576197A (en) * 1995-04-07 1996-11-19 Molecular Bio-Products Polymerase chain reaction container and methods of using the same
US5837442A (en) 1995-11-29 1998-11-17 Roche Molecular Systems, Inc. Oligonucleotide primers for amplifying HCV nucleic acid
US5721123A (en) * 1996-01-05 1998-02-24 Microfab Technology, Inc. Methods and apparatus for direct heating of biological material
US5763184A (en) 1996-01-30 1998-06-09 Roche Molecular Systems, Inc. Nucleotide sequence variation in the ABO glycosyltransferase gene
EP2264045B1 (en) 1996-08-14 2015-10-21 Life Technologies Corporation Stable compositions for nucleic acid amplification and sequencing
JP3182527B2 (ja) * 1996-09-03 2001-07-03 株式会社ヤトロン 化学発光の測定方法及びキット
US6110710A (en) * 1996-10-15 2000-08-29 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Sequence modification of oligonucleotide primers to manipulate non-templated nucleotide addition
EP2258846A3 (en) * 1997-07-07 2012-03-21 Medical Research Council A method for increasing the concentration of a nucleic acid molecule
US6893877B2 (en) 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
US8337753B2 (en) 1998-05-01 2012-12-25 Gen-Probe Incorporated Temperature-controlled incubator having a receptacle mixing mechanism
EP2156891A3 (en) * 1998-05-01 2010-06-02 Gen-Probe Incorporated System and method for incubating the contents of a reaction receptacle
US6183998B1 (en) 1998-05-29 2001-02-06 Qiagen Gmbh Max-Volmer-Strasse 4 Method for reversible modification of thermostable enzymes
US20020055118A1 (en) * 1998-06-24 2002-05-09 Yong-Bin Eym Method of preparing objects containing DNA
ES2153745B1 (es) * 1998-07-31 2001-11-16 Ivia Dispositivo compartimentado y metodo para la captura y doble amplificacion enzimatica de secuencias diana de acidos nucleicos en un solo dispositivo compartimentado cerrado.
US6413780B1 (en) 1998-10-14 2002-07-02 Abbott Laboratories Structure and method for performing a determination of an item of interest in a sample
US6200757B1 (en) 1999-01-19 2001-03-13 Dade Behring Inc. Method for controlling the extension of an oligonucleotide
NZ513390A (en) 1999-03-19 2003-01-31 Genencor Int Multi-through hole testing plate for high throughput screening
DE60014399T2 (de) 1999-05-04 2005-12-08 Ortho-Clinical Diagnostics, Inc. Schnelle und wirksame dns-immobilisierung aus einer probe ohne die verwendung von zelllysereagenzien
US6643787B1 (en) 1999-10-19 2003-11-04 Rambus Inc. Bus system optimization
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
US7262006B1 (en) 2000-05-01 2007-08-28 Ortho-Clinical Diagnostics, Inc. Rapid and efficient capture of DNA from sample without using cell lysing reagent
WO2002012263A1 (en) * 2000-08-03 2002-02-14 Roche Diagnostics Gmbh Nucleic acid binding compounds containing pyrazolo[3,4-d]pyrimidine analogues of purin-2,6-diamine and their uses
WO2002057414A2 (en) * 2000-10-20 2002-07-25 Expression Diagnostics, Inc. Leukocyte expression profiling
WO2002034905A1 (fr) * 2000-10-27 2002-05-02 Supercritical Co., Ltd. Procédé d'amplification de gènes
FR2817961B1 (fr) * 2000-12-08 2003-08-01 Diagast Barriere de separation temporaire, recipient la comprenant et procede de mise en oeuvre d'un test dans ce recipient
US6428962B1 (en) 2001-02-12 2002-08-06 Dna Analysis, Inc. Nucleic acid collection barrier method and apparatus
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6905827B2 (en) * 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
US7026121B1 (en) * 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
EP1396538A4 (en) * 2001-06-12 2005-10-26 Takara Bio Inc METHOD FOR STABILIZING A REAGENT FOR AMPLIFICATION OR FOR DETECTING NUCLEIC ACID AND STORAGE PROCEDURES
US7226732B2 (en) * 2001-07-16 2007-06-05 Cepheid Methods, apparatus, and computer programs for verifying the integrity of a probe
US6403341B1 (en) 2001-08-02 2002-06-11 Wayne M. Barnes Magnesium precipitate hot start method for PCR
US20030027196A1 (en) * 2001-08-02 2003-02-06 Barnes Wayne M. Magnesium precipitate methods for magnesium dependent enzymes
WO2004011666A2 (en) * 2002-07-26 2004-02-05 Applera Corporation Mg-mediated hot start biochemical reactions
US8277753B2 (en) * 2002-08-23 2012-10-02 Life Technologies Corporation Microfluidic transfer pin
EP1403379A1 (en) * 2002-09-24 2004-03-31 QIAGEN GmbH Enhanced coamplification of nucleic acids
US7682565B2 (en) * 2002-12-20 2010-03-23 Biotrove, Inc. Assay apparatus and method using microfluidic arrays
JP4473878B2 (ja) * 2003-01-29 2010-06-02 ４５４ コーポレーション 核酸を増幅および配列決定する方法
DE10315640A1 (de) * 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
WO2004094986A2 (en) * 2003-04-16 2004-11-04 Handylab, Inc. System and method for electrochemical detection of biological compounds
US7892745B2 (en) * 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
EP1953246A3 (en) 2003-05-01 2008-11-19 Veridex, LLC Intraoperative lymph node assay
WO2004101733A1 (fr) * 2003-05-01 2004-11-25 Southeast University Dispositif d'amplification pcr pour reaction a etapes multiples et tube d'amplification pcr sans lavage pour detection de genes
DE502004011280D1 (de) * 2004-01-23 2010-07-29 Qiagen Gmbh Kontaminationsbarriere
JP2007518425A (ja) * 2004-01-23 2007-07-12 バイオメリュー・インコーポレイテッド Ｈｃｖ３’非翻訳領域を効率的に増幅および検出するためのプライマーおよびプローブの設計
JP2007529015A (ja) 2004-03-12 2007-10-18 バイオトローブ， インコーポレイテッド ナノリットルのアレイローディング
DE102004021822B3 (de) * 2004-04-30 2005-11-17 Siemens Ag Verfahren und Anordnung zur DNA-Amplifikation mittels PCR unter Einsatz von Trockenreagenzien
NZ551430A (en) * 2004-06-04 2010-01-29 Veridex Llc Diagnosing or predicting the course of breast cancer
WO2005123913A2 (en) * 2004-06-09 2005-12-29 Nustar Laboratory Reversibly modified thermostable enzyme compositions and methods of making and using the same
US20070154889A1 (en) * 2004-06-25 2007-07-05 Veridex, Llc Methods and reagents for the detection of melanoma
US7700281B2 (en) * 2004-06-30 2010-04-20 Usb Corporation Hot start nucleic acid amplification
GB0414815D0 (en) * 2004-07-02 2004-08-04 Secr Defence Method for stabilising reagents which are useful for nucleic acid amplification
GB2416352B (en) * 2004-07-21 2009-01-28 Bioline Ltd A method for performing the hot start of enzymatic reactions
US20060029801A1 (en) * 2004-08-03 2006-02-09 O'brien Robert Method for making a coated powder for reducing evaporative water loss
WO2006029184A2 (en) * 2004-09-08 2006-03-16 Expression Diagnostics, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
EP1871527B1 (en) * 2004-12-23 2017-09-27 Abbott Point of Care Inc. Molecular diagnostics system
JP4580981B2 (ja) * 2005-03-30 2010-11-17 株式会社島津製作所 遺伝子多型診断用装置
EP1885889A4 (en) * 2005-05-11 2010-01-20 Expression Diagnostics Inc METHOD FOR MONITORING THE OPERATING STATUS OF TRANSPLANTS OVER GENLISTS
WO2007002588A2 (en) * 2005-06-23 2007-01-04 Applera Corporation Thermal-cycling pipette tip
WO2007011903A2 (en) 2005-07-15 2007-01-25 Applera Corporation Analyzing messenger rna and micro rna in the same reaction mixture
EP2405024A1 (en) 2005-07-15 2012-01-11 Life Technologies Corporation Hot start reverse transcription by primer design
US7754148B2 (en) 2006-12-27 2010-07-13 Progentech Limited Instrument for cassette for sample preparation
US7727473B2 (en) 2005-10-19 2010-06-01 Progentech Limited Cassette for sample preparation
GB0600228D0 (en) 2006-01-06 2006-02-15 Fermentas Uab Inactivation method
ES2364983T3 (es) * 2006-02-27 2011-09-20 F. Hoffmann-La Roche Ag Reacción en cadena de polimerasa con arranque en caliente por secuestro de magnesio.
EP3167900B1 (en) 2006-03-29 2018-11-21 Merial Limited Vaccine against streptococci
WO2008051290A2 (en) * 2006-04-07 2008-05-02 Xdx, Inc. Steroid responsive nucleic acid expression and prediction of disease activity
US8334099B2 (en) * 2006-07-31 2012-12-18 Wanli Bi Nucleic acid amplification using a reversibly modified oligonucleotide
US9045522B2 (en) 2006-07-31 2015-06-02 Wanli Bi Nucleic acid amplification using a reversibly modified oligonucleotide
US7993832B2 (en) * 2006-08-14 2011-08-09 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
EP2102367A2 (en) * 2006-11-09 2009-09-23 XDX, Inc. Methods for diagnosing and monitoring the status of systemic lupus erythematosus
WO2008065996A1 (fr) * 2006-11-28 2008-06-05 Shimadzu Corporation Plaque de réaction
JP4307478B2 (ja) * 2006-12-05 2009-08-05 キヤノン株式会社 生化学反応用カートリッジ
US9938641B2 (en) * 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
WO2008122843A2 (en) * 2007-04-10 2008-10-16 Pontes Luis Andre Use of liquid barriers to perform hot start pcr
WO2008134470A2 (en) * 2007-04-25 2008-11-06 3M Innovative Properties Company Methods for nucleic acid amplification
JPWO2008146649A1 (ja) * 2007-05-25 2010-08-19 ベックマン・コールター・インコーポレーテッド 酵素含有ゲル及び核酸の増幅キット
JPWO2008146686A1 (ja) * 2007-05-25 2010-08-19 ベックマン・コールター・インコーポレーテッド 酵素含有カプセル及び核酸の増幅キット
EP2183380B1 (en) 2007-08-27 2014-11-26 Life Technologies Corporation Methods and compositions for pcr
WO2009054473A1 (ja) * 2007-10-26 2009-04-30 Toppan Printing Co., Ltd. 反応チップ及び反応方法、遺伝子処理装置用温度調節機構及び遺伝子処理装置
CA2658520C (en) 2008-03-19 2016-11-08 F. Hoffmann-La Roche Ag Nucleic acid amplification in the presence of modified randomers
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
AU2009242546B2 (en) 2008-04-30 2015-01-22 Integrated Dna Technologies, Inc. RNase-H-based assays utilizing modified RNA monomers
EP2990487A1 (en) 2008-05-08 2016-03-02 Asuragen, INC. Compositions and methods related to mirna modulation of neovascularization or angiogenesis
US7910720B2 (en) * 2008-09-09 2011-03-22 Roche Diagnostics Operations, Inc. Polyanion for improved nucleic acid amplification
IT1397110B1 (it) * 2008-12-29 2012-12-28 St Microelectronics Rousset Microreattore autosigillante e metodo per eseguire una reazione
JP5608997B2 (ja) * 2009-03-31 2014-10-22 東洋紡株式会社 保存安定性に優れた核酸増幅検出試薬キット
JP5608998B2 (ja) * 2009-03-31 2014-10-22 東洋紡株式会社 保存安定性に優れた核酸増幅検出試薬キット
US20100261229A1 (en) * 2009-04-08 2010-10-14 Applied Biosystems, Llc System and method for preparing and using bulk emulsion
AU2010249421A1 (en) 2009-05-22 2011-12-08 Asuragen, Inc. miRNA biomarkers of prostate disease
IL206810A (en) * 2009-07-08 2015-07-30 Janssen Diagnostics Llc Methods for identifying melanoma and kits for using these methods
JP5229160B2 (ja) * 2009-08-26 2013-07-03 株式会社島津製作所 反応容器
EP2470897A4 (en) 2009-08-28 2013-05-29 Asuragen Inc MICRO-RNA BIOMARKERS OF PULMONARY DISEASE
WO2011060014A1 (en) * 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
US9238832B2 (en) 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
US8614071B2 (en) 2009-12-11 2013-12-24 Roche Molecular Systems, Inc. Preferential amplification of mRNA over DNA using chemically modified primers
US9248422B2 (en) 2010-02-23 2016-02-02 Luminex Corporation Apparatus and methods for integrated sample preparation, reaction and detection
KR101643196B1 (ko) 2010-02-23 2016-07-27 루미넥스 코포레이션 일체화된 샘플 제조, 반응 및 검출을 위한 장치 및 방법
SI23321A (sl) 2010-03-03 2011-09-30 Inštitut za fizikalno biologijo d.o.o. Izboljĺ ano zbiranje fluorescentne svetlobe iz vzorca med pomnoĺ˝evanjem v rt-pcr
DK201000356A (en) * 2010-04-22 2011-10-23 Statens Seruminstitut New nucleotide sequence amplification Method
JP5454338B2 (ja) * 2010-04-28 2014-03-26 株式会社島津製作所 液滴操作によるリアルタイム核酸増幅法
CA2798123C (en) 2010-05-05 2020-06-23 The Governing Council Of The University Of Toronto Method of processing dried samples using digital microfluidic device
CN102947448A (zh) * 2010-06-22 2013-02-27 环球生物研究株式会社 用于防止核酸扩增反应中的反应溶液的蒸发的组合物
EP2596135A2 (en) 2010-07-23 2013-05-29 Beckman Coulter, Inc. System and method including analytical units
US20120045799A1 (en) * 2010-08-17 2012-02-23 Rheonix, Inc. Thermocycler seal composition, method, and application
EP2640851A2 (en) 2010-11-17 2013-09-25 Asuragen, Inc. Mirnas as biomarkers for distinguishing benign from malignant thyroid neoplasms
AU2012236896A1 (en) 2011-03-25 2013-05-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
US9469871B2 (en) 2011-04-14 2016-10-18 Corporos Inc. Methods and apparatus for point-of-care nucleic acid amplification and detection
US8911941B2 (en) 2011-04-14 2014-12-16 Kenneth J. Michlitsch Methods and apparatus for point-of-care nucleic acid amplification and detection
US20120288866A1 (en) * 2011-05-11 2012-11-15 Genturadx, Inc. Systems and methods for producing an evaporation barrier in a reaction chamber
US8475739B2 (en) 2011-09-25 2013-07-02 Theranos, Inc. Systems and methods for fluid handling
US20140170735A1 (en) 2011-09-25 2014-06-19 Elizabeth A. Holmes Systems and methods for multi-analysis
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
US9664702B2 (en) 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
KR20140064971A (ko) 2011-09-19 2014-05-28 제넨테크, 인크. c-met 길항제 및 B-raf 길항제를 포함하는 조합 치료
CN103797132A (zh) 2011-09-23 2014-05-14 霍夫曼-拉罗奇有限公司 G形夹用于改进的等位基因特异性pcr的用途
US10012664B2 (en) 2011-09-25 2018-07-03 Theranos Ip Company, Llc Systems and methods for fluid and component handling
US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
US9115394B2 (en) 2011-12-22 2015-08-25 Roche Molecular Systems, Inc. Methods and reagents for reducing non-specific amplification
GB2561425B (en) 2012-03-16 2019-01-16 Cambridge Entpr Ltd Apparatus for obtaining liquid from a solid phase
US9354159B2 (en) 2012-05-02 2016-05-31 Nanoscopia (Cayman), Inc. Opto-fluidic system with coated fluid channels
US9085761B1 (en) 2012-06-14 2015-07-21 Affymetrix, Inc. Methods and compositions for amplification of nucleic acids
DE102012222351A1 (de) 2012-12-05 2014-06-05 Gna Biosolutions Gmbh Reaktionsgefäß mit magnetischem Verschluss
CA2907377A1 (en) 2013-03-15 2014-09-18 Baylor Research Institute Tissue and blood-based mirna biomarkers for the diagnosis, prognosis and metastasis-predictive potential in colorectal cancer
WO2014151551A1 (en) 2013-03-15 2014-09-25 Baylor Research Institute Ulcerative colitis (uc)-associated colorectal neoplasia markers
US11545241B1 (en) 2013-09-07 2023-01-03 Labrador Diagnostics Llc Systems and methods for analyte testing and data management
WO2015101653A1 (en) 2014-01-05 2015-07-09 Biomirna Holdings Ltd Lung cancer determinations using mirna ratios
US10619210B2 (en) 2014-02-07 2020-04-14 The Johns Hopkins University Predicting response to epigenetic drug therapy
US9937494B2 (en) 2014-06-16 2018-04-10 Life Technologies Corporation Reagent mixer and fluid control devices
CN110327993B (zh) * 2014-06-17 2021-07-13 生命技术公司 测序装置
JP2017527288A (ja) 2014-09-04 2017-09-21 セラノス， インコーポレイテッドＴｈｅｒａｎｏｓ， Ｉｎｃ． 病原体及び抗菌薬耐性試験
WO2016094330A2 (en) 2014-12-08 2016-06-16 20/20 Genesystems, Inc Methods and machine learning systems for predicting the liklihood or risk of having cancer
US9909169B2 (en) 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
CN104845967B (zh) 2015-04-15 2020-12-11 苏州新海生物科技股份有限公司 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用
ES2961374T3 (es) 2015-04-24 2024-03-11 Atila Biosystems Incorporated Amplificación con cebadores de composición de nucleótidos limitada
EP3292214A1 (en) 2015-05-04 2018-03-14 Academisch Medisch Centrum Method of quantifying mirnas using normalization
EP3303548A4 (en) 2015-06-05 2019-01-02 Miroculus Inc. Evaporation management in digital microfluidic devices
CN108026494A (zh) 2015-06-05 2018-05-11 米罗库鲁斯公司 限制蒸发和表面结垢的空气基质数字微流控装置和方法
US10576473B2 (en) 2015-08-31 2020-03-03 Siemens Healthcare Diagnostics Inc. Method and device for prevention of splashing of non-capped fluid sample during transport on diagnostic laboratory Equipment
AU2017214675B2 (en) * 2016-02-05 2022-11-24 Gen-Probe Incorporated Dried amplification compositions
WO2017223026A1 (en) * 2016-06-20 2017-12-28 Miroculus Inc. Detection of rna using ligation actuated loop mediated amplification methods and digital microfluidics
CA3034064A1 (en) 2016-08-22 2018-03-01 Miroculus Inc. Feedback system for parallel droplet control in a digital microfluidic device
US10427162B2 (en) 2016-12-21 2019-10-01 Quandx Inc. Systems and methods for molecular diagnostics
CA3049416A1 (en) 2016-12-28 2018-07-05 Miroculus Inc. Digital microfluidic devices and methods
US11623219B2 (en) 2017-04-04 2023-04-11 Miroculus Inc. Digital microfluidics apparatuses and methods for manipulating and processing encapsulated droplets
US20180320218A1 (en) * 2017-05-08 2018-11-08 Smobio Technology, Inc. Kit for polymerase chain reaction
US11413617B2 (en) 2017-07-24 2022-08-16 Miroculus Inc. Digital microfluidics systems and methods with integrated plasma collection device
CA3073058A1 (en) 2017-09-01 2019-03-07 Miroculus Inc. Digital microfluidics devices and methods of using them
CN112469504A (zh) 2018-05-23 2021-03-09 米罗库鲁斯公司 对数字微流控中的蒸发的控制
EP3719144A1 (en) 2019-04-05 2020-10-07 Fundación para la Investigación Biomédica del Hospital Universitario de la Paz (FIBHULP) Mir-151a-3p as an universal endogenous control for exosome cargo normalization
EP3953041A4 (en) 2019-04-08 2023-01-25 Miroculus Inc. MULTIPLE CARTRIDGE DIGITAL MICROFLUIDIC APPARATUS AND METHODS OF USE
WO2021013592A1 (en) 2019-07-19 2021-01-28 Fundación Para La Investigación Biomédica Del Hospital Universitario La Paz (Fibhulp) Method for determining the response to treatment of a patient affected by non-small cell lung carcinoma (nsclc)
US11524298B2 (en) 2019-07-25 2022-12-13 Miroculus Inc. Digital microfluidics devices and methods of use thereof
CN113373202A (zh) * 2020-03-10 2021-09-10 苏州先达基因科技有限公司 防污染单管核酸恒温扩增检测***
CN114657236B (zh) * 2020-12-23 2024-06-18 广州达安基因股份有限公司 一种分隔核酸扩增体系的方法和分隔剂
CN113680406B (zh) * 2021-08-26 2022-04-15 清华大学 一种微流控芯片多指标检测方法
US11857961B2 (en) 2022-01-12 2024-01-02 Miroculus Inc. Sequencing by synthesis using mechanical compression

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE100617C (es) *
US3919085A (en) * 1974-02-27 1975-11-11 Becton Dickinson Co Plasma separator assembly
US4032492A (en) * 1976-02-25 1977-06-28 H. B. Fuller Company Hot melt container closure sealant
AU520409B2 (en) * 1977-05-25 1982-01-28 Commonwealth Scientific And Industrial Research Organisation Controlled release composition
US4522786A (en) * 1983-08-10 1985-06-11 E. I. Du Pont De Nemours And Company Multilayered test device for detecting analytes in liquid test samples
DE3416094A1 (de) * 1984-04-30 1985-10-31 Hilti Ag, Schaan Zerstoerbare patrone fuer mehrkomponenten-massen
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
CA1338457C (en) * 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
EP0357611B1 (en) * 1987-02-26 1995-04-26 The University Of Sydney A method of detection of carcinogenic human papillomavirus
CA1338505C (en) * 1989-02-03 1996-08-06 John Bruce Findlay Containment cuvette for pcr and method of use

Also Published As

Publication number Publication date
JPH05506143A (ja) 1993-09-16
WO1991012342A1 (en) 1991-08-22
DE69131891T2 (de) 2000-06-15
AU7307391A (en) 1991-09-03
DE69131891D1 (de) 2000-02-10
EP0515506B1 (en) 2000-01-05
JP3532826B2 (ja) 2004-05-31
ATE188509T1 (de) 2000-01-15
EP0515506A1 (en) 1992-12-02
US5411876A (en) 1995-05-02
CA2075050A1 (en) 1991-08-17
JP3087907B2 (ja) 2000-09-18
DK0515506T3 (da) 2000-05-08
CA2075050C (en) 1998-10-06
JP2000316561A (ja) 2000-11-21
AU653712B2 (en) 1994-10-13

Similar Documents

Publication Publication Date Title
ES2141088T3 (es) Mejoras en la especificidad y conveniencia de la reaccion en cadena de la polimerasa.
Fakruddin et al. Nucleic acid amplification: Alternative methods of polymerase chain reaction
Lowery et al. Characterization of the nucleoside triphosphate phosphohydrolase (ATPase) activity of RNA synthesis termination factor p. II. Influence of synthetic RNA homopolymers and random copolymers on the reaction.
Malorny et al. Standardization of diagnostic PCR for the detection of foodborne pathogens
Koo et al. CRISPR/dCas9-mediated biosensor for detection of tick-borne diseases
Thompson et al. Developmental validation of the PowerPlex® Y23 System: a single multiplex Y-STR analysis system for casework and database samples
Morris et al. Reconstruction of bacteriophage T4 DNA replication apparatus from purified components: rolling circle replication following de novo chain initiation on a single-stranded circular DNA template.
DE3789849D1 (de) Replikative rns-rapporteur-systeme.
AU5442896A (en) Methods and reagents for combined PCR amplification and hybridization probing assay
CA2130108A1 (en) Heat-Releasable Reagents for in Vitro Chemical Reactions
KR930700681A (ko) 생체외 증폭을 이용한 폴리뉴클레오티드 검체분석
WO1999060159A3 (en) Method for using unequal primer concentrations for generating nucleic acid amplification products
WO2006076017A2 (en) Methods and kits for identifying target nucleotides in mixed populations
DK0726324T3 (da) Anvendelse af exonuklease og/eller glycosylase som supplementer til antipolymeraseantistof til forøgelse af specification i polymerasekædereaktion
MX9701479A (es) Metodo de marcado con lucifrasa.
KR900016473A (ko) 진단 키트, 프라이머 조성물 및 이것을 이용해 핵산을 복제하거나 검사하는 방법
KR910006497A (ko) 프라이머 3' 말단에서의 프라이머-표적 오합(mismatch)을 극복한 진단 및 증폭방법
WO2001092569A3 (en) Formulation for polymerase chain reaction and vessel containing same
EP0875582A3 (en) Process for HLA Class 1 DNA typing using PCR amplification
Havliš et al. 5-Methylcytosine as a marker for the monitoring of DNA methylation
KR920700294A (ko) 개선된 프라이머 연장반응
Chang et al. A stimulatory factor for yeast DNA polymerase
DeWit et al. Simple method for production of internal control DNA for Mycobacterium tuberculosis polymerase chain reaction assays
EP0826779A1 (en) Strand displacement amplification using boronated nucleotides
Tews et al. Application of the C4'-alkylated deoxyribose primer system (CAPS) in allele-specific real-time PCR for increased selectivity in discrimination of single nucleotide sequence variants

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 515506

Country of ref document: ES