ES2118534T3 - Escalera de marcadores de acido nucleico para estimacion de la masa. - Google Patents
Escalera de marcadores de acido nucleico para estimacion de la masa.Info
- Publication number
- ES2118534T3 ES2118534T3 ES95901092T ES95901092T ES2118534T3 ES 2118534 T3 ES2118534 T3 ES 2118534T3 ES 95901092 T ES95901092 T ES 95901092T ES 95901092 T ES95901092 T ES 95901092T ES 2118534 T3 ES2118534 T3 ES 2118534T3
- Authority
- ES
- Spain
- Prior art keywords
- nucleic acid
- staircase
- estimation
- mass
- restriction endonucleases
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 4
- 102000039446 nucleic acids Human genes 0.000 title abstract 4
- 108020004707 nucleic acids Proteins 0.000 title abstract 4
- 239000003550 marker Substances 0.000 title abstract 2
- 108091008146 restriction endonucleases Proteins 0.000 abstract 4
- 239000012634 fragment Substances 0.000 abstract 2
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Enzymes And Modification Thereof (AREA)
- Sampling And Sample Adjustment (AREA)
- Road Signs Or Road Markings (AREA)
Abstract
LA INVENCION SE REFIERE A UNA ESCALA MARCADORA DE ACIDO NUCLEICO QUE ES UN DIGESTO DE ENDONUCLEASAS DE RESTRICCION, EN DONDE UN DIGESTO DE ENDONUCLEASAS DE RESTRICCION DE ACIDO NUCLEICO ES UNA COLECCION DE FRAGMENTOS DE ACIDO NUCLEICO QUE RESULTAN DE LA DIGESTION COMPLETA DE UNO O MAS ACIDOS NUCLEICOS POR UNA O MAS ENDONUCLEASAS DE RESTRICCION; EL DIGESTO DE ENDONUCLEASAS DE RESTRICCION CONTIENE AL MENOS TRES FRAGMENTOS; Y EL TAMAÑO DE LOS FRAGMENTOS EN LOS PARES DE BASE ES MULTIPLO DE UN ENTERO, EN DONDE EL ENTERO ES 10 O MAYOR.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US14212493A | 1993-10-28 | 1993-10-28 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2118534T3 true ES2118534T3 (es) | 1998-09-16 |
Family
ID=22498632
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES95901092T Expired - Lifetime ES2118534T3 (es) | 1993-10-28 | 1994-10-28 | Escalera de marcadores de acido nucleico para estimacion de la masa. |
Country Status (7)
Country | Link |
---|---|
US (1) | US5834201A (es) |
EP (1) | EP0725821B1 (es) |
AT (1) | ATE168413T1 (es) |
DE (1) | DE69411753T2 (es) |
DK (1) | DK0725821T3 (es) |
ES (1) | ES2118534T3 (es) |
WO (1) | WO1995011971A1 (es) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6680378B1 (en) | 1993-10-28 | 2004-01-20 | Invitrogen Corporation | Nucleic acid marker ladder for estimating mass |
ES2308800T3 (es) | 1997-01-08 | 2008-12-01 | Invitrogen Corporation | Metodos para la produccion de proteinas. |
WO1998044156A1 (en) | 1997-03-27 | 1998-10-08 | Life Technologies, Inc. | Nucleic acid ladders |
EP1001963B1 (en) * | 1997-07-15 | 2011-01-05 | Life Technologies, Inc. | Nucleic acid ladders |
US6054276A (en) | 1998-02-23 | 2000-04-25 | Macevicz; Stephen C. | DNA restriction site mapping |
CA2362771A1 (en) * | 1999-03-12 | 2000-09-21 | Amersham Pharmacia Biotech Uk Limited | Genetic analysis |
WO2001079553A1 (en) * | 2000-04-14 | 2001-10-25 | Lynx Therapeutics, Inc. | Method and compositions for ordering restriction fragments |
JP2004506221A (ja) * | 2000-08-11 | 2004-02-26 | インヴィトロジェン コーポレーション | 高度に均一な電気泳動用分子マーカー |
US20030157720A1 (en) * | 2002-02-06 | 2003-08-21 | Expression Technologies Inc. | Protein standard for estimating size and mass |
AU2003201778A1 (en) * | 2003-01-13 | 2004-08-10 | Seegene, Inc. | Dna size markers and method for preparing them |
US20060183205A1 (en) * | 2003-01-29 | 2006-08-17 | Laurent Masaro | Method for controlling molecular weight and distribution of biopolymers |
EG24237A (en) * | 2005-08-09 | 2008-11-11 | Mubarak City For Scient Res | Method for preparation of dna ladder using pcr andits optimization by numerical modeling thereof |
CN106011133B (zh) * | 2016-03-16 | 2019-04-26 | 北京博迈德基因工程技术有限公司 | 一种小的dna分子量标准物、标准物质粒及其制备方法 |
CN106399296A (zh) * | 2016-09-05 | 2017-02-15 | 公安部物证鉴定中心 | 一种标准分子量片段混合物的制备方法及由其制备的标准分子量片段混合物 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4403036A (en) * | 1980-12-02 | 1983-09-06 | University Of Iowa Research Foundation | Genetic reagents for generating plasmids containing multiple copies of DNA segments |
US4682195A (en) * | 1985-09-30 | 1987-07-21 | General Electric Company | Insulated gate device with configured emitter contact pad |
US5162514A (en) * | 1990-05-15 | 1992-11-10 | Board Of Regents, University Of Texas | High molecular weight dna compositions for use in electrophoresis of large nucleic acids |
US5316908A (en) * | 1990-07-13 | 1994-05-31 | Life Technologies, Inc. | Size markers for electrophoretic analysis of DNA |
US5137814A (en) * | 1991-06-14 | 1992-08-11 | Life Technologies, Inc. | Use of exo-sample nucleotides in gene cloning |
WO1993014224A1 (en) * | 1992-01-14 | 1993-07-22 | Applied Biosystems, Inc. | Size-calibration dna fragment mixture and method |
-
1994
- 1994-10-28 ES ES95901092T patent/ES2118534T3/es not_active Expired - Lifetime
- 1994-10-28 AT AT95901092T patent/ATE168413T1/de not_active IP Right Cessation
- 1994-10-28 WO PCT/US1994/012505 patent/WO1995011971A1/en active IP Right Grant
- 1994-10-28 DE DE69411753T patent/DE69411753T2/de not_active Expired - Lifetime
- 1994-10-28 EP EP95901092A patent/EP0725821B1/en not_active Expired - Lifetime
- 1994-10-28 DK DK95901092T patent/DK0725821T3/da active
-
1997
- 1997-07-11 US US08/893,523 patent/US5834201A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
US5834201A (en) | 1998-11-10 |
EP0725821A1 (en) | 1996-08-14 |
WO1995011971A1 (en) | 1995-05-04 |
EP0725821B1 (en) | 1998-07-15 |
DE69411753D1 (de) | 1998-08-20 |
ATE168413T1 (de) | 1998-08-15 |
DE69411753T2 (de) | 1999-02-04 |
DK0725821T3 (da) | 1999-02-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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