ES2118534T3 - Escalera de marcadores de acido nucleico para estimacion de la masa. - Google Patents

Escalera de marcadores de acido nucleico para estimacion de la masa.

Info

Publication number
ES2118534T3
ES2118534T3 ES95901092T ES95901092T ES2118534T3 ES 2118534 T3 ES2118534 T3 ES 2118534T3 ES 95901092 T ES95901092 T ES 95901092T ES 95901092 T ES95901092 T ES 95901092T ES 2118534 T3 ES2118534 T3 ES 2118534T3
Authority
ES
Spain
Prior art keywords
nucleic acid
staircase
estimation
mass
restriction endonucleases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95901092T
Other languages
English (en)
Inventor
James L Hartley
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Inc
Original Assignee
Life Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Inc filed Critical Life Technologies Inc
Application granted granted Critical
Publication of ES2118534T3 publication Critical patent/ES2118534T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Road Signs Or Road Markings (AREA)

Abstract

LA INVENCION SE REFIERE A UNA ESCALA MARCADORA DE ACIDO NUCLEICO QUE ES UN DIGESTO DE ENDONUCLEASAS DE RESTRICCION, EN DONDE UN DIGESTO DE ENDONUCLEASAS DE RESTRICCION DE ACIDO NUCLEICO ES UNA COLECCION DE FRAGMENTOS DE ACIDO NUCLEICO QUE RESULTAN DE LA DIGESTION COMPLETA DE UNO O MAS ACIDOS NUCLEICOS POR UNA O MAS ENDONUCLEASAS DE RESTRICCION; EL DIGESTO DE ENDONUCLEASAS DE RESTRICCION CONTIENE AL MENOS TRES FRAGMENTOS; Y EL TAMAÑO DE LOS FRAGMENTOS EN LOS PARES DE BASE ES MULTIPLO DE UN ENTERO, EN DONDE EL ENTERO ES 10 O MAYOR.
ES95901092T 1993-10-28 1994-10-28 Escalera de marcadores de acido nucleico para estimacion de la masa. Expired - Lifetime ES2118534T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US14212493A 1993-10-28 1993-10-28

Publications (1)

Publication Number Publication Date
ES2118534T3 true ES2118534T3 (es) 1998-09-16

Family

ID=22498632

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95901092T Expired - Lifetime ES2118534T3 (es) 1993-10-28 1994-10-28 Escalera de marcadores de acido nucleico para estimacion de la masa.

Country Status (7)

Country Link
US (1) US5834201A (es)
EP (1) EP0725821B1 (es)
AT (1) ATE168413T1 (es)
DE (1) DE69411753T2 (es)
DK (1) DK0725821T3 (es)
ES (1) ES2118534T3 (es)
WO (1) WO1995011971A1 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6680378B1 (en) 1993-10-28 2004-01-20 Invitrogen Corporation Nucleic acid marker ladder for estimating mass
ES2308800T3 (es) 1997-01-08 2008-12-01 Invitrogen Corporation Metodos para la produccion de proteinas.
WO1998044156A1 (en) 1997-03-27 1998-10-08 Life Technologies, Inc. Nucleic acid ladders
EP1001963B1 (en) * 1997-07-15 2011-01-05 Life Technologies, Inc. Nucleic acid ladders
US6054276A (en) 1998-02-23 2000-04-25 Macevicz; Stephen C. DNA restriction site mapping
CA2362771A1 (en) * 1999-03-12 2000-09-21 Amersham Pharmacia Biotech Uk Limited Genetic analysis
WO2001079553A1 (en) * 2000-04-14 2001-10-25 Lynx Therapeutics, Inc. Method and compositions for ordering restriction fragments
JP2004506221A (ja) * 2000-08-11 2004-02-26 インヴィトロジェン　コーポレーション 高度に均一な電気泳動用分子マーカー
US20030157720A1 (en) * 2002-02-06 2003-08-21 Expression Technologies Inc. Protein standard for estimating size and mass
AU2003201778A1 (en) * 2003-01-13 2004-08-10 Seegene, Inc. Dna size markers and method for preparing them
US20060183205A1 (en) * 2003-01-29 2006-08-17 Laurent Masaro Method for controlling molecular weight and distribution of biopolymers
EG24237A (en) * 2005-08-09 2008-11-11 Mubarak City For Scient Res Method for preparation of dna ladder using pcr andits optimization by numerical modeling thereof
CN106011133B (zh) * 2016-03-16 2019-04-26 北京博迈德基因工程技术有限公司 一种小的dna分子量标准物、标准物质粒及其制备方法
CN106399296A (zh) * 2016-09-05 2017-02-15 公安部物证鉴定中心 一种标准分子量片段混合物的制备方法及由其制备的标准分子量片段混合物

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4403036A (en) * 1980-12-02 1983-09-06 University Of Iowa Research Foundation Genetic reagents for generating plasmids containing multiple copies of DNA segments
US4682195A (en) * 1985-09-30 1987-07-21 General Electric Company Insulated gate device with configured emitter contact pad
US5162514A (en) * 1990-05-15 1992-11-10 Board Of Regents, University Of Texas High molecular weight dna compositions for use in electrophoresis of large nucleic acids
US5316908A (en) * 1990-07-13 1994-05-31 Life Technologies, Inc. Size markers for electrophoretic analysis of DNA
US5137814A (en) * 1991-06-14 1992-08-11 Life Technologies, Inc. Use of exo-sample nucleotides in gene cloning
WO1993014224A1 (en) * 1992-01-14 1993-07-22 Applied Biosystems, Inc. Size-calibration dna fragment mixture and method

Also Published As

Publication number Publication date
US5834201A (en) 1998-11-10
EP0725821A1 (en) 1996-08-14
WO1995011971A1 (en) 1995-05-04
EP0725821B1 (en) 1998-07-15
DE69411753D1 (de) 1998-08-20
ATE168413T1 (de) 1998-08-15
DE69411753T2 (de) 1999-02-04
DK0725821T3 (da) 1999-02-15

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