ES2093717T3 - Mutante de hirudina, su produccion, anticoagulante, vector secretor, microorganismo transformado por el indicado vector y produccion de un producto a partir de dicho microorganismo. - Google Patents
Mutante de hirudina, su produccion, anticoagulante, vector secretor, microorganismo transformado por el indicado vector y produccion de un producto a partir de dicho microorganismo.Info
- Publication number
- ES2093717T3 ES2093717T3 ES91919149T ES91919149T ES2093717T3 ES 2093717 T3 ES2093717 T3 ES 2093717T3 ES 91919149 T ES91919149 T ES 91919149T ES 91919149 T ES91919149 T ES 91919149T ES 2093717 T3 ES2093717 T3 ES 2093717T3
- Authority
- ES
- Spain
- Prior art keywords
- production
- vector
- microorganism
- hirudin
- hirudine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/33—Fusion polypeptide fusions for targeting to specific cell types, e.g. tissue specific targeting, targeting of a bacterial subspecies
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
UN MUTANTE HIRUDINO HVIC3, PREPARADO SUBSTITUYENDO UNA SECUENCIA DE AMINOACIDOS, QUE EMPIEZA CON EL AMINOACIDO 53, RESIDUO DEL HIRUDINO HV1, POR OTRA SECUENCIA DE AMINOACIDOS QUE EMPIEZA CON EL AMINOACIDO 53, RESIDUO DEL HIRUDINO HV3. DICHO MUTANTE NO SOLO TIENE UN ALTO EFECTO ANTITROMBO SINO QUE TAMBIEN SIRVE PARA INHIBIR EL ALARGAMIENTO DEL TIEMPO DE SANGRADO. ESCHERICHIA COLI TRANSFORMADA POR UN VECTOR SEGREGANTE DE PROTEINA EXTRAÑA, COMPRENDIENDO UNA SECUENCIA DE ADN QUE CONTIENE UN ORIGEN (ORI) DE PLASMIDO PUC, UNA SECUENCIA ADN DE PROMOTOR TAC O PROMOTOR TRP, UNA SECUENCIA ADN CODIFICADA COMO PEPTIDO DE SEÑAL Y UNA SECUENCIA ADN CODIFICADA COMO PROTEINA EXTRAÑA, QUE SEGREGA Y PRODUCE PROTEINAS EXTRAÑAS EN GRANDES CANTIDADES FUERA DE LAS CELULAS.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2303096A JPH07119237B2 (ja) | 1990-11-08 | 1990-11-08 | ヒルジン変異体,その製造法及び抗凝血剤 |
JP4127191A JP3226289B2 (ja) | 1991-02-13 | 1991-02-13 | 分泌ベクター、該ベクターで形質転換した微生物及び該微生物から産生される産物の製造法 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2093717T3 true ES2093717T3 (es) | 1997-01-01 |
Family
ID=26380836
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES95202092T Expired - Lifetime ES2129749T3 (es) | 1990-11-08 | 1991-11-08 | Vector de secrecion, microorganismos transformados que contienen el indicado vector y produccion de un producto a partir de dicho microorganismo. |
ES91919149T Expired - Lifetime ES2093717T3 (es) | 1990-11-08 | 1991-11-08 | Mutante de hirudina, su produccion, anticoagulante, vector secretor, microorganismo transformado por el indicado vector y produccion de un producto a partir de dicho microorganismo. |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES95202092T Expired - Lifetime ES2129749T3 (es) | 1990-11-08 | 1991-11-08 | Vector de secrecion, microorganismos transformados que contienen el indicado vector y produccion de un producto a partir de dicho microorganismo. |
Country Status (12)
Country | Link |
---|---|
US (2) | US5516656A (es) |
EP (2) | EP0511393B1 (es) |
AT (2) | ATE176500T1 (es) |
AU (2) | AU648124B2 (es) |
CA (2) | CA2255396A1 (es) |
DE (2) | DE69130872T2 (es) |
DK (2) | DK0687731T3 (es) |
ES (2) | ES2129749T3 (es) |
FI (1) | FI107928B (es) |
GR (2) | GR3021410T3 (es) |
NO (2) | NO303735B1 (es) |
WO (1) | WO1992008736A1 (es) |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0158564B1 (fr) * | 1984-03-27 | 1992-07-15 | Transgene S.A. | Vecteurs d'expression de l'hirudine, cellules transformées et procédé de préparation de l'hirudine |
DE4009268A1 (de) * | 1990-03-22 | 1991-09-26 | Consortium Elektrochem Ind | Sekretion von hirudinderivaten |
US5972648A (en) * | 1993-09-28 | 1999-10-26 | Japan Energy Corporation | Hirudin analogs, methods of manufacture thereof and anticoagulant compositions having these as active ingredients |
CA2205356A1 (en) | 1994-11-21 | 1996-05-30 | Peter Edward Urwin | Modified proteinase inhibitors |
DE19944870A1 (de) | 1999-09-18 | 2001-03-29 | Aventis Pharma Gmbh | Signalsequenzen zur Herstellung von Leu-Hirudin über Sekretion durch E. coli in das Kulturmedium |
DE10108211A1 (de) * | 2001-02-20 | 2002-08-22 | Aventis Pharma Gmbh | Verwendung von Fusionsproteinen, deren N-terminaler Anteil aus einem Hirudinderivat besteht, zur Herstellung rekombinanter Proteine über Sekretion durch Hefen |
US7202059B2 (en) | 2001-02-20 | 2007-04-10 | Sanofi-Aventis Deutschland Gmbh | Fusion proteins capable of being secreted into a fermentation medium |
DE10108212A1 (de) * | 2001-02-20 | 2002-08-22 | Aventis Pharma Gmbh | Fusionsprotein zur Sekretion von Wertprotein in bakterielle Überstände |
US7638618B2 (en) | 2001-02-20 | 2009-12-29 | Sanofi-Aventis Deutschland Gmbh | Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest |
KR100447530B1 (ko) * | 2001-08-14 | 2004-09-08 | 한국과학기술원 | OmpF를 이용하여 목적 단백질을 대장균 세포외로분비생산하는 방법 |
KR20050058494A (ko) | 2002-08-23 | 2005-06-16 | 이 아이 듀폰 디 네모아 앤드 캄파니 | 발효에 의한 생물학적 제조를 위한 전분 생성물의 이용 |
JP2006096668A (ja) * | 2002-11-08 | 2006-04-13 | Ono Pharmaceut Co Ltd | エラスターゼ阻害剤と血液凝固系および/または線溶系酵素阻害剤との組み合わせからなる医薬 |
US7795205B2 (en) * | 2004-04-12 | 2010-09-14 | Canyon Pharmaceuticals, Inc. | Methods for effecting regression of tumor mass and size in a metastasized pancreatic tumor |
EP1737889B1 (en) | 2004-10-19 | 2010-09-08 | Lonza AG | Method for solid phase peptide synthesis |
DE502006009060D1 (de) * | 2006-09-22 | 2011-04-21 | Wacker Chemie Ag | Verfahren zur fermentativen Herstellung von Antikörpern |
CN101372512B (zh) * | 2007-08-23 | 2011-03-23 | 中国人民解放军军事医学科学院生物工程研究所 | 一类抗凝血多肽及其用途 |
WO2010054503A1 (zh) * | 2008-11-17 | 2010-05-20 | 中国人民解放军军事医学科学院生物工程研究所 | 抗凝血多肽及其应用 |
CN115572329B (zh) * | 2021-06-21 | 2024-02-06 | 王大勇 | 一组活性增强代谢较慢的菲牛蛭基因重组水蛭素及其制备方法 |
Family Cites Families (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2562088B1 (fr) * | 1984-03-27 | 1987-11-13 | Transgene Sa | Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine |
DE3586386T2 (de) * | 1984-10-05 | 1993-01-14 | Genentech Inc | Dna, zellkulturen und verfahren zur sekretion von heterologen proteinen und periplasmische proteinrueckgewinnung. |
DE3445532A1 (de) * | 1984-12-13 | 1986-06-19 | Plantorgan Werk Heinrich G.E. Christensen, KG, 2903 Bad Zwischenahn | Hirudin-pa, desulfatohirudine-pa, verfahren zur herstellung und pharmazeutische mittel, die diese wirkstoffe enthalten |
DE3506992A1 (de) * | 1985-02-27 | 1986-08-28 | Plantorgan Werk Heinrich G.E. Christensen, KG, 2903 Bad Zwischenahn | Modifizierte hirudine, verfahren zu deren herstellung und pharmazeutische mittel, die diese wirkstoffe enthalten |
FR2607517B2 (fr) * | 1986-12-01 | 1989-12-22 | Transgene Sa | Vecteurs d'expression de variants de l'hirudine dans les levures, procede et produit obtenu |
US5084384A (en) * | 1987-04-23 | 1992-01-28 | Monsanto Company | Secretion of insulin-like growth factor-I |
JP2518862B2 (ja) * | 1987-08-31 | 1996-07-31 | 株式会社蛋白工学研究所 | 新規プラスミドベクタ― |
AU604925B2 (en) * | 1988-02-23 | 1991-01-03 | Schering Aktiengesellschaft | A hirudin derivative |
AU614121B2 (en) | 1988-05-04 | 1991-08-22 | Novartis Ag | Improvements in the production of polypeptides |
EP0347376B1 (en) * | 1988-06-11 | 1994-03-23 | Ciba-Geigy Ag | Novel polypeptides with an anticoagulant activity |
US5268296A (en) * | 1988-06-11 | 1993-12-07 | Ciba-Geigy Corporation | DNA vector and recombinant host cell for production of hirullin P6 and P18 |
JPH0227993A (ja) * | 1988-07-15 | 1990-01-30 | Nippon Shinyaku Co Ltd | hEGFの製法 |
JPH0648988B2 (ja) | 1988-07-26 | 1994-06-29 | 工業技術院長 | トロンビン阻害物質の製造法 |
US5223407A (en) * | 1988-08-31 | 1993-06-29 | Allelix Inc. | Excretion of heterologous proteins from e. coli |
JPH0292288A (ja) * | 1988-09-30 | 1990-04-03 | Japan Tobacco Inc | アクアライシンi前駆体をコードする遺伝子、それを含む発現ベクター、該発現ベクターを含む大腸菌及びそれを用いたアクアライシンiの製造方法 |
DE3835815A1 (de) * | 1988-10-21 | 1990-04-26 | Hoechst Ag | Neue isohirudine |
JPH03164184A (ja) | 1989-08-10 | 1991-07-16 | Nippon Mining Co Ltd | ヒルジン発現ベクター、ヒルジン融合蛋白、形質転換微生物及びヒルジンの製造法 |
EP0412526A3 (en) * | 1989-08-10 | 1991-03-06 | Nippon Mining Company Limited | Expression vector for hirudin, hirudin fusion protein, transformed microorganism, and method for production of hirudin |
JPH0376579A (ja) | 1989-08-18 | 1991-04-02 | Nippon Mining Co Ltd | ヒト‐アンジオジェニン発現ベクター及びヒト‐アンジオジェニンの製造方法 |
GB9010552D0 (en) * | 1990-05-10 | 1990-07-04 | Erba Carlo Spa | Method for the recombinant production of hirudins and novel hirudins |
-
1991
- 1991-11-08 AU AU88466/91A patent/AU648124B2/en not_active Ceased
- 1991-11-08 DK DK95202092T patent/DK0687731T3/da active
- 1991-11-08 ES ES95202092T patent/ES2129749T3/es not_active Expired - Lifetime
- 1991-11-08 EP EP91919149A patent/EP0511393B1/en not_active Expired - Lifetime
- 1991-11-08 CA CA002255396A patent/CA2255396A1/en not_active Abandoned
- 1991-11-08 AT AT95202092T patent/ATE176500T1/de not_active IP Right Cessation
- 1991-11-08 ES ES91919149T patent/ES2093717T3/es not_active Expired - Lifetime
- 1991-11-08 DE DE69130872T patent/DE69130872T2/de not_active Expired - Fee Related
- 1991-11-08 DE DE69121192T patent/DE69121192T2/de not_active Expired - Fee Related
- 1991-11-08 EP EP95202092A patent/EP0687731B1/en not_active Expired - Lifetime
- 1991-11-08 AT AT91919149T patent/ATE140929T1/de not_active IP Right Cessation
- 1991-11-08 CA CA002072375A patent/CA2072375C/en not_active Expired - Fee Related
- 1991-11-08 WO PCT/JP1991/001533 patent/WO1992008736A1/ja active IP Right Grant
- 1991-11-08 DK DK91919149.4T patent/DK0511393T3/da active
-
1992
- 1992-06-26 FI FI922963A patent/FI107928B/fi active
- 1992-07-07 NO NO922671A patent/NO303735B1/no not_active IP Right Cessation
- 1992-07-08 US US07/910,528 patent/US5516656A/en not_active Expired - Fee Related
-
1994
- 1994-01-25 AU AU54701/94A patent/AU673870B2/en not_active Ceased
- 1994-11-28 US US08/348,972 patent/US5573929A/en not_active Expired - Fee Related
-
1996
- 1996-10-23 GR GR960402781T patent/GR3021410T3/el unknown
-
1998
- 1998-05-14 NO NO982207A patent/NO982207D0/no not_active Application Discontinuation
-
1999
- 1999-03-30 GR GR990400915T patent/GR3029824T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
AU648124B2 (en) | 1994-04-14 |
WO1992008736A1 (fr) | 1992-05-29 |
NO922671L (no) | 1992-09-07 |
EP0511393B1 (en) | 1996-07-31 |
CA2255396A1 (en) | 1992-05-09 |
US5516656A (en) | 1996-05-14 |
CA2072375C (en) | 2000-01-11 |
AU8846691A (en) | 1992-06-11 |
NO982207L (no) | 1992-09-07 |
NO303735B1 (no) | 1998-08-24 |
DK0687731T3 (da) | 1999-09-20 |
DE69121192T2 (de) | 1997-02-20 |
GR3021410T3 (en) | 1997-01-31 |
AU5470194A (en) | 1994-03-24 |
DK0511393T3 (da) | 1996-12-09 |
FI922963A (fi) | 1992-06-26 |
EP0511393A4 (en) | 1993-08-11 |
DE69130872D1 (de) | 1999-03-18 |
ES2129749T3 (es) | 1999-06-16 |
FI107928B (fi) | 2001-10-31 |
EP0687731A1 (en) | 1995-12-20 |
AU673870B2 (en) | 1996-11-28 |
DE69130872T2 (de) | 1999-08-26 |
GR3029824T3 (en) | 1999-06-30 |
NO982207D0 (no) | 1998-05-14 |
NO922671D0 (no) | 1992-07-07 |
DE69121192D1 (de) | 1996-09-05 |
ATE140929T1 (de) | 1996-08-15 |
US5573929A (en) | 1996-11-12 |
FI922963A0 (fi) | 1992-06-26 |
EP0511393A1 (en) | 1992-11-04 |
ATE176500T1 (de) | 1999-02-15 |
EP0687731B1 (en) | 1999-02-03 |
CA2072375A1 (en) | 1992-05-09 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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