JPS56109597A - Detection with luciferin analog - Google Patents

Detection with luciferin analog

Info

Publication number
JPS56109597A
JPS56109597A JP1001380A JP1001380A JPS56109597A JP S56109597 A JPS56109597 A JP S56109597A JP 1001380 A JP1001380 A JP 1001380A JP 1001380 A JP1001380 A JP 1001380A JP S56109597 A JPS56109597 A JP S56109597A
Authority
JP
Japan
Prior art keywords
luciferin analog
enzyme
immobilized
photocell
test tube
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP1001380A
Other languages
Japanese (ja)
Inventor
Shoichi Shimizu
Takeshi Kobayashi
Kunio Omiya
Kengo Saga
Toshio Goto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to JP1001380A priority Critical patent/JPS56109597A/en
Publication of JPS56109597A publication Critical patent/JPS56109597A/en
Pending legal-status Critical Current

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  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: When a substrate is detected utilizing the luminous property of luciferin analog, an immobilized enzyme is employed, thus permitting high-sensitivity detection with simple operations.
CONSTITUTION: Enzymes immobilized on a membrane is prepared from a combination of xanthin oxidase or peroxidase and uricase or glucose oxidase using of a photocuring resin e.g. by the overall immobilization method or the like. The enzyme-immobilized membrane is placed in test tube 2 and set in dark box 3 so that it opposes through slit plate 5 to photocell 4. Then, luciferin analog and a sample to be tested are placed in the test tube 2 and enzyme reactions are effected. As the reactions progress, the luciferin analog emits light, which is detected by the photocell 4 and quantitatively recorded on the recorder.
COPYRIGHT: (C)1981,JPO&Japio
JP1001380A 1980-02-01 1980-02-01 Detection with luciferin analog Pending JPS56109597A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP1001380A JPS56109597A (en) 1980-02-01 1980-02-01 Detection with luciferin analog

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP1001380A JPS56109597A (en) 1980-02-01 1980-02-01 Detection with luciferin analog

Publications (1)

Publication Number Publication Date
JPS56109597A true JPS56109597A (en) 1981-08-31

Family

ID=11738505

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1001380A Pending JPS56109597A (en) 1980-02-01 1980-02-01 Detection with luciferin analog

Country Status (1)

Country Link
JP (1) JPS56109597A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63106543A (en) * 1986-08-06 1988-05-11 キャンベラ、インダストリ−ズ、インコ−ポレ−テッド Usage of oxidase enzyme system in chemical luminescence analysis
JP2017513001A (en) * 2014-04-07 2017-05-25 インスティティ・パスツールＩｎｓｔｉｔｕｔ Ｐａｓｔｅｕｒ Enzyme-independent photon emission

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63106543A (en) * 1986-08-06 1988-05-11 キャンベラ、インダストリ−ズ、インコ−ポレ−テッド Usage of oxidase enzyme system in chemical luminescence analysis
JPH0716438B2 (en) * 1986-08-06 1995-03-01 キャンベラ、インダストリ−ズ、インコ−ポレ−テッド Use of the oxidase enzyme system in chemiluminescence analysis
JP2017513001A (en) * 2014-04-07 2017-05-25 インスティティ・パスツールＩｎｓｔｉｔｕｔ Ｐａｓｔｅｕｒ Enzyme-independent photon emission

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