ES2036234T3 - Un metodo para determinar la actividad de la isoenzima dl-1 de fluidos biologicos. - Google Patents

Un metodo para determinar la actividad de la isoenzima dl-1 de fluidos biologicos.

Info

Publication number
ES2036234T3
ES2036234T3 ES198888107865T ES88107865T ES2036234T3 ES 2036234 T3 ES2036234 T3 ES 2036234T3 ES 198888107865 T ES198888107865 T ES 198888107865T ES 88107865 T ES88107865 T ES 88107865T ES 2036234 T3 ES2036234 T3 ES 2036234T3
Authority
ES
Spain
Prior art keywords
isoenzyme
biological fluids
activity
chaotropic agent
ldh
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES198888107865T
Other languages
English (en)
Inventor
William A. Abbott
Rita Sue Byrne
David Anthony Yost
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Application granted granted Critical
Publication of ES2036234T3 publication Critical patent/ES2036234T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Abstract

UN METODO RAPIDO Y ESPECIFICO Y EL REACTIVO APROPIADO PARA DETERMINAR LD-1 ISOENZIMA EN FLUIDOS BIOLOGICOS POR INCORPORACION DE UN AGENTE CAOTROPICO, TAL COMO PERCLORATO DE SODIO, EN UN SISTEMA DE REACTIVO E ENSAYO LDH. EL AGENTE CAOTROPICO PRODUCE UNA INACTIVACION INMEDIATA DE LAS ISOENZIMAS LDH QUE CONTIENEN UNA O MAS SUBUNIDADES TIPO M MIENTRAS QUE LA ISOENZIMA LD-1 PERMANECE ESTABLE Y SE MIDE SIMULTANEAMENTE.
ES198888107865T 1987-05-28 1988-05-17 Un metodo para determinar la actividad de la isoenzima dl-1 de fluidos biologicos. Expired - Lifetime ES2036234T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US5510787A 1987-05-28 1987-05-28

Publications (1)

Publication Number Publication Date
ES2036234T3 true ES2036234T3 (es) 1993-05-16

Family

ID=21995651

Family Applications (1)

Application Number Title Priority Date Filing Date
ES198888107865T Expired - Lifetime ES2036234T3 (es) 1987-05-28 1988-05-17 Un metodo para determinar la actividad de la isoenzima dl-1 de fluidos biologicos.

Country Status (7)

Country Link
EP (1) EP0292838B1 (es)
JP (1) JPH0698031B2 (es)
AT (1) ATE80665T1 (es)
AU (1) AU611438B2 (es)
CA (1) CA1320418C (es)
DE (1) DE3874611T2 (es)
ES (1) ES2036234T3 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6242208B1 (en) 1988-07-15 2001-06-05 International Reagents Corporation LDH1 assay
JPH0775556B2 (ja) * 1988-07-15 1995-08-16 国際試薬株式会社 Ｌｄｈアイソザイムのｌｄｈ▲下１▼の分別定量法
AU605417B2 (en) * 1989-04-18 1991-01-10 John Lysaght (Australia) Limited A structural spacer
EP0401641B1 (en) * 1989-06-09 1995-01-25 Abbott Laboratories Method for assaying isoenzymes
IE920779A1 (en) * 1991-03-13 1992-09-23 Du Pont Selective stabilization of lactate dehydrogenase isoenzyme¹ld1 by high molecular weight polyols
US20060014136A1 (en) * 2004-07-14 2006-01-19 Inimex Pharmaceuticals, Inc. Method of screening for protection from microbial infection
CN114381494B (zh) * 2021-12-01 2023-12-22 天津中成佳益生物科技有限公司 一种乳酸脱氢酶同工酶1的检测试剂及检测方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3388044A (en) * 1965-12-07 1968-06-11 Warner Lambert Pharmaceutical Process for differentiating the isoenzymes of lactic dehydrogenase
DE2513407A1 (de) * 1975-03-26 1976-10-14 Schmidt Karlheinz Ein verfahren zur differenzierung der leber- und herzspezifischen lactatdehydrogenase im blutserum
US4250255A (en) * 1977-07-11 1981-02-10 Eastman Kodak Company Assay method for isoenzyme activity

Also Published As

Publication number Publication date
JPS6463397A (en) 1989-03-09
EP0292838A1 (en) 1988-11-30
ATE80665T1 (de) 1992-10-15
DE3874611T2 (de) 1993-04-15
DE3874611D1 (de) 1992-10-22
JPH0698031B2 (ja) 1994-12-07
AU611438B2 (en) 1991-06-13
EP0292838B1 (en) 1992-09-16
AU1659388A (en) 1988-12-01
CA1320418C (en) 1993-07-20

Similar Documents

Publication Publication Date Title
DK249085D0 (da) Testmateriale til bestemmelse af naervaerelsen af ethanol i en vandig proeve, dets fremstilling og anvendelse
ATE19895T1 (de) Stabilisierung von oxidase.
JPS57146599A (en) Test composition, test kit and test method for measuring glucose in liquid test sample
NO179953C (no) Kvantitativ metode for bestemmelse av 1,4-dihydronikotinamid-adenin-dinukleotid i opplösning, samt engangs enzymelektrode
ES2036234T3 (es) Un metodo para determinar la actividad de la isoenzima dl-1 de fluidos biologicos.
ATE37199T1 (de) Enzymatische bestimmung von harnstoff.
DE3784528D1 (de) Zusammensetzung und verfahren zur stabilisierung von dinukleotiden.
DE3473475D1 (en) Process for determining substrate or enzymatic activity
ES557404A0 (es) Procedimiento de dosificacion de anticuerpos dirigidos contra toxinas bacterianas.
PT83826B (pt) Processo para a preparacao dum reagente para a determinacao da actividade de peroxidase
KR890008327A (ko) 효소 역학의 자동측정 방법
Kilburn et al. A study of human platelet esterases
DK16384A (da) Fremgangsmaade til bestemmelse af deoxythymidinkinase (dtk)isoenzymaktivitet
FR2439995A1 (fr) Procede et dispositif de mesure de la concentration d'une solution en substrat oxydable par reaction enzymatique
JPS5567653A (en) Measuring method of methotrexate concentration in body fluids and combination of measuring reagent
ES8205317A1 (es) Procedimiento para la determinacion de hemoglobina.
IT1222604B (it) Metodo colorimetrico per la determinazione quantitativa di magnesio con l'uso di alfa-glicerofosfato-ossidasi

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 292838

Country of ref document: ES