EP3623477A4 - RECOMBINANT VECTOR WITH CELLULOSE BINDING DOMAIN AND METHOD OF ISOLATION AND PURIFICATION OF PROTEIN USING THE SAME VECTOR - Google Patents

RECOMBINANT VECTOR WITH CELLULOSE BINDING DOMAIN AND METHOD OF ISOLATION AND PURIFICATION OF PROTEIN USING THE SAME VECTOR Download PDF

Info

Publication number
EP3623477A4
EP3623477A4 EP18798939.7A EP18798939A EP3623477A4 EP 3623477 A4 EP3623477 A4 EP 3623477A4 EP 18798939 A EP18798939 A EP 18798939A EP 3623477 A4 EP3623477 A4 EP 3623477A4
Authority
EP
European Patent Office
Prior art keywords
isolating
binding domain
cellulose binding
purifying protein
recombinant vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP18798939.7A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP3623477A2 (en
Inventor
Inhwan Hwang
Eun Ju Sohn
Yongjik Lee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bioapplications Inc
Original Assignee
Bioapplications Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioapplications Inc filed Critical Bioapplications Inc
Publication of EP3623477A2 publication Critical patent/EP3623477A2/en
Publication of EP3623477A4 publication Critical patent/EP3623477A4/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1019—Tetrapeptides with the first amino acid being basic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
EP18798939.7A 2017-05-11 2018-05-10 RECOMBINANT VECTOR WITH CELLULOSE BINDING DOMAIN AND METHOD OF ISOLATION AND PURIFICATION OF PROTEIN USING THE SAME VECTOR Pending EP3623477A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020170058882A KR101848082B1 (ko) 2017-05-11 2017-05-11 셀룰로오즈 결합 도메인을 포함하는 재조합 벡터 및 상기 벡터를 사용하여 단백질을 분리정제하는 방법
PCT/KR2018/005371 WO2018208099A2 (ko) 2017-05-11 2018-05-10 셀룰로오즈 결합 도메인을 포함하는 재조합 벡터 및 상기 벡터를 사용하여 단백질을 분리정제하는 방법

Publications (2)

Publication Number Publication Date
EP3623477A2 EP3623477A2 (en) 2020-03-18
EP3623477A4 true EP3623477A4 (en) 2021-02-17

Family

ID=61969336

Family Applications (1)

Application Number Title Priority Date Filing Date
EP18798939.7A Pending EP3623477A4 (en) 2017-05-11 2018-05-10 RECOMBINANT VECTOR WITH CELLULOSE BINDING DOMAIN AND METHOD OF ISOLATION AND PURIFICATION OF PROTEIN USING THE SAME VECTOR

Country Status (6)

Country Link
US (1) US10934558B2 (zh)
EP (1) EP3623477A4 (zh)
JP (1) JP6945883B2 (zh)
KR (1) KR101848082B1 (zh)
CN (1) CN110709518B (zh)
WO (1) WO2018208099A2 (zh)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101848082B1 (ko) 2017-05-11 2018-04-12 주식회사 바이오앱 셀룰로오즈 결합 도메인을 포함하는 재조합 벡터 및 상기 벡터를 사용하여 단백질을 분리정제하는 방법
KR102138272B1 (ko) 2018-09-19 2020-07-28 주식회사 바이오앱 BiP 단편을 포함하는 재조합 벡터 및 상기 벡터를 이용한 재조합 단백질의 제조 방법
KR102288367B1 (ko) 2018-11-15 2021-08-11 주식회사 바이오앱 식물체에서 바이러스-유사 입자를 발현하는 재조합 벡터 및 이를 이용한 써코바이러스-유사 입자를 포함하는 백신 조성물의 제조방법
WO2020101187A1 (ko) 2018-11-15 2020-05-22 주식회사 바이오앱 식물체에서 바이러스-유사 입자를 발현하는 재조합 벡터 및 이를 이용한 써코바이러스-유사 입자를 포함하는 백신 조성물의 제조방법
KR102250576B1 (ko) 2018-11-15 2021-05-12 주식회사 바이오앱 식물 발현 시스템에서 캡시드 단백질의 발현을 증가시키는 재조합 벡터 및 이를 이용한 바이러스-유사 입자의 제조방법
KR102317403B1 (ko) * 2019-01-28 2021-10-29 주식회사 바이오앱 당화된 Ag85A 단백질을 포함하는 결핵 예방용 백신 조성물 및 이의 제조방법
KR102213745B1 (ko) * 2019-04-16 2021-02-09 주식회사 바이오앱 돼지 유행성 설사병 바이러스 백신 조성물 및 이의 제조 방법
CN112458109B (zh) * 2020-11-11 2023-07-04 西北农林科技大学 一种基于负压与暂时浸没的高效瞬时植物转基因方法
KR20220122815A (ko) 2021-02-25 2022-09-05 주식회사 바이오앱 식물 발현 시스템을 이용한 알팔파 모자이크 바이러스 유사 입자 제조 방법 및 이의 활용
WO2023195555A1 (ko) * 2022-04-06 2023-10-12 포항공과대학교 산학협력단 식물에서 tgfbeta1 재조합 단백질의 생산 방법

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005021762A2 (en) * 2003-08-27 2005-03-10 Orf Liftaekni Hf. A process for proteolytic cleavage and purification of recombinant proteins produced in plants
WO2005023981A2 (en) * 2003-09-09 2005-03-17 Zephyr Proteomix Ltd. Libraries of chimeric cellulose binding proteins and methods of use thereof

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11225763A (ja) * 1998-02-12 1999-08-24 Toyobo Co Ltd タンパク質の精製方法
ES2224792B1 (es) * 2002-06-28 2007-02-16 Era Plantech, S.L. Produccion de peptidos y proteinas por acumulacion de cuerpos proteicos derivados de reticulos endoplasmico en plantas.
KR100618563B1 (ko) * 2003-10-29 2006-08-31 한국생명공학연구원 셀룰로오스 결합 도메인을 이용한 효모에서 재조합단백질의 제조방법
KR101161622B1 (ko) * 2009-08-31 2012-07-04 헬릭스 주식회사 번역 효율 증진용 ｄｎａ 단편 및 이를 포함하는 재조합 벡터
JP5641478B2 (ja) * 2010-07-18 2014-12-17 独立行政法人国際農林水産業研究センター 酵素の再利用方法
KR20130130563A (ko) * 2012-05-22 2013-12-02 (재) 스크립스코리아항체연구원 전체 세포 분리 방법
KR101507207B1 (ko) * 2012-10-05 2015-03-31 주식회사 바이오앱 식물에서 셀룰로오즈와 셀룰로오즈 결합 도메인을 사용하여 단백질을 분리정제하는 방법
KR101848082B1 (ko) 2017-05-11 2018-04-12 주식회사 바이오앱 셀룰로오즈 결합 도메인을 포함하는 재조합 벡터 및 상기 벡터를 사용하여 단백질을 분리정제하는 방법

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005021762A2 (en) * 2003-08-27 2005-03-10 Orf Liftaekni Hf. A process for proteolytic cleavage and purification of recombinant proteins produced in plants
WO2005023981A2 (en) * 2003-09-09 2005-03-17 Zephyr Proteomix Ltd. Libraries of chimeric cellulose binding proteins and methods of use thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ERIN EGELKROUT ET AL: "Overproduction of recombinant proteins in plants", PLANT SCIENCE, ELSEVIER IRELAND LTD, IE, vol. 184, 9 December 2011 (2011-12-09), pages 83 - 101, XP028452706, ISSN: 0168-9452, [retrieved on 20111216], DOI: 10.1016/J.PLANTSCI.2011.12.005 *
ULLRICH KRISTIAN K ET AL: "Means to optimize protein expression in transgenic plants", CURRENT OPINION IN BIOTECHNOLOGY, LONDON, GB, vol. 32, 25 November 2014 (2014-11-25), pages 61 - 67, XP029584739, ISSN: 0958-1669, DOI: 10.1016/J.COPBIO.2014.11.011 *

Also Published As

Publication number Publication date
US20200270624A1 (en) 2020-08-27
KR101848082B1 (ko) 2018-04-12
JP2020519264A (ja) 2020-07-02
CN110709518A (zh) 2020-01-17
EP3623477A2 (en) 2020-03-18
JP6945883B2 (ja) 2021-10-06
WO2018208099A3 (ko) 2019-01-10
CN110709518B (zh) 2023-08-15
WO2018208099A2 (ko) 2018-11-15
US10934558B2 (en) 2021-03-02

Similar Documents

Publication Publication Date Title
EP3623477A4 (en) RECOMBINANT VECTOR WITH CELLULOSE BINDING DOMAIN AND METHOD OF ISOLATION AND PURIFICATION OF PROTEIN USING THE SAME VECTOR
EP3330290A4 (en) ONE DOMAIN ANTIBODY FOR THE LIGANDING OF PROGRAMMED CELL DEATH (PD-L1) AND DERIVED PROTEIN THEREOF
EP3426689A4 (en) INDUCTIBLE BINDING PROTEINS AND METHODS OF USE
EP3637940A4 (en) DISCONNECTING PROCEDURE, APPROPRIATE DEVICE AND SYSTEM
EP3674312A4 (en) NOVEL POLYPEPTIDE AND METHOD OF MANUFACTURING IMP WITH USING THEREOF
EP3414962A4 (en) SYSTEM AND METHOD FOR MULTIPLE USER INTEGRATED DUPLEX LINK ADAPTATION
EP3963565A4 (en) CONTENT DELIVERY SYSTEM AND PROCEDURES
EP3689893A4 (en) IMMUNOGLOBULIN-BINDING PROTEIN AND AFFINITY CARRIERS THEREFORE
EP3411068A4 (en) BISPECIFIC BINDING PROTEINS FOR PD-L1 AND KDR
EP3858064A4 (en) SYSTEM AND PROCEDURE FOR ACTIVATING DL-EDT
EP3613772A4 (en) SPECIFIC BINDING MOLECULE FOR LRIG-1 PROTEIN AND ITS USE
EP3475809A4 (en) SYSTEM AND METHOD FOR USING VIRTUAL VECTOR REGISTER FILES
EP3582806A4 (en) HER2, NKG2D AND CD16 BOUND PROTEINS
EP3276918A4 (en) DOWNLOAD MODE SWITCHING METHOD, AND CONTROL METHOD AND CONTROL SYSTEM THEREFOR
EP3309702A4 (en) Method and system for securely switching terminal between product mode and development mode
EP3583131A4 (en) PROTEINS THAT BIND CD33, NKG2D AND CD16
EP3574245A4 (en) EXHAUST CLAMP AND PROCESS
EP3541848A4 (en) PROTEINLIGATION METHOD AND USES THEREOF
EP3689894A4 (en) IMMUNOGLOBULIN-BINDING PROTEIN AND AFFINITY CARRIERS THEREFORE
EP3689903A4 (en) FIBRONECTIN B DOMAIN BINDING PROTEIN
EP3572507A4 (en) EXPRESSION METHOD FOR RECOMBINANT HAEMOCOAGULASE ACUTUS PROTEIN
EP3521299A4 (en) PROCESS FOR PURIFICATION OF RECOMBINANT PROTEIN
EP4063483A4 (en) MODIFIED CYANOBACTERIA, METHOD FOR PRODUCING MODIFIED CYANOBACTERIA AND METHOD FOR PRODUCING PROTEINS
EP3583133A4 (en) GD2, NKG2D AND CD16 BINDING PROTEINS
EP3813995A4 (en) HEMP PROTEIN AND ITS USE FOR MICROENCAPSULATION

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20191114

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

AX Request for extension of the european patent

Extension state: BA ME

DAV Request for validation of the european patent (deleted)
DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20210118

RIC1 Information provided on ipc code assigned before grant

Ipc: C07K 1/22 20060101ALI20210112BHEP

Ipc: C12N 15/82 20060101AFI20210112BHEP

Ipc: C07K 14/35 20060101ALI20210112BHEP

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: EXAMINATION IS IN PROGRESS

17Q First examination report despatched

Effective date: 20240221