EP3623477A4 - RECOMBINANT VECTOR WITH CELLULOSE BINDING DOMAIN AND METHOD OF ISOLATION AND PURIFICATION OF PROTEIN USING THE SAME VECTOR - Google Patents
RECOMBINANT VECTOR WITH CELLULOSE BINDING DOMAIN AND METHOD OF ISOLATION AND PURIFICATION OF PROTEIN USING THE SAME VECTOR Download PDFInfo
- Publication number
- EP3623477A4 EP3623477A4 EP18798939.7A EP18798939A EP3623477A4 EP 3623477 A4 EP3623477 A4 EP 3623477A4 EP 18798939 A EP18798939 A EP 18798939A EP 3623477 A4 EP3623477 A4 EP 3623477A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- isolating
- binding domain
- cellulose binding
- purifying protein
- recombinant vector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229920002678 cellulose Polymers 0.000 title 1
- 239000001913 cellulose Substances 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
- C07K5/10—Tetrapeptides
- C07K5/1019—Tetrapeptides with the first amino acid being basic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8205—Agrobacterium mediated transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Gastroenterology & Hepatology (AREA)
- Immunology (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020170058882A KR101848082B1 (ko) | 2017-05-11 | 2017-05-11 | 셀룰로오즈 결합 도메인을 포함하는 재조합 벡터 및 상기 벡터를 사용하여 단백질을 분리정제하는 방법 |
PCT/KR2018/005371 WO2018208099A2 (ko) | 2017-05-11 | 2018-05-10 | 셀룰로오즈 결합 도메인을 포함하는 재조합 벡터 및 상기 벡터를 사용하여 단백질을 분리정제하는 방법 |
Publications (2)
Publication Number | Publication Date |
---|---|
EP3623477A2 EP3623477A2 (en) | 2020-03-18 |
EP3623477A4 true EP3623477A4 (en) | 2021-02-17 |
Family
ID=61969336
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP18798939.7A Pending EP3623477A4 (en) | 2017-05-11 | 2018-05-10 | RECOMBINANT VECTOR WITH CELLULOSE BINDING DOMAIN AND METHOD OF ISOLATION AND PURIFICATION OF PROTEIN USING THE SAME VECTOR |
Country Status (6)
Country | Link |
---|---|
US (1) | US10934558B2 (zh) |
EP (1) | EP3623477A4 (zh) |
JP (1) | JP6945883B2 (zh) |
KR (1) | KR101848082B1 (zh) |
CN (1) | CN110709518B (zh) |
WO (1) | WO2018208099A2 (zh) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101848082B1 (ko) | 2017-05-11 | 2018-04-12 | 주식회사 바이오앱 | 셀룰로오즈 결합 도메인을 포함하는 재조합 벡터 및 상기 벡터를 사용하여 단백질을 분리정제하는 방법 |
KR102138272B1 (ko) | 2018-09-19 | 2020-07-28 | 주식회사 바이오앱 | BiP 단편을 포함하는 재조합 벡터 및 상기 벡터를 이용한 재조합 단백질의 제조 방법 |
KR102288367B1 (ko) | 2018-11-15 | 2021-08-11 | 주식회사 바이오앱 | 식물체에서 바이러스-유사 입자를 발현하는 재조합 벡터 및 이를 이용한 써코바이러스-유사 입자를 포함하는 백신 조성물의 제조방법 |
WO2020101187A1 (ko) | 2018-11-15 | 2020-05-22 | 주식회사 바이오앱 | 식물체에서 바이러스-유사 입자를 발현하는 재조합 벡터 및 이를 이용한 써코바이러스-유사 입자를 포함하는 백신 조성물의 제조방법 |
KR102250576B1 (ko) | 2018-11-15 | 2021-05-12 | 주식회사 바이오앱 | 식물 발현 시스템에서 캡시드 단백질의 발현을 증가시키는 재조합 벡터 및 이를 이용한 바이러스-유사 입자의 제조방법 |
KR102317403B1 (ko) * | 2019-01-28 | 2021-10-29 | 주식회사 바이오앱 | 당화된 Ag85A 단백질을 포함하는 결핵 예방용 백신 조성물 및 이의 제조방법 |
KR102213745B1 (ko) * | 2019-04-16 | 2021-02-09 | 주식회사 바이오앱 | 돼지 유행성 설사병 바이러스 백신 조성물 및 이의 제조 방법 |
CN112458109B (zh) * | 2020-11-11 | 2023-07-04 | 西北农林科技大学 | 一种基于负压与暂时浸没的高效瞬时植物转基因方法 |
KR20220122815A (ko) | 2021-02-25 | 2022-09-05 | 주식회사 바이오앱 | 식물 발현 시스템을 이용한 알팔파 모자이크 바이러스 유사 입자 제조 방법 및 이의 활용 |
WO2023195555A1 (ko) * | 2022-04-06 | 2023-10-12 | 포항공과대학교 산학협력단 | 식물에서 tgfbeta1 재조합 단백질의 생산 방법 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005021762A2 (en) * | 2003-08-27 | 2005-03-10 | Orf Liftaekni Hf. | A process for proteolytic cleavage and purification of recombinant proteins produced in plants |
WO2005023981A2 (en) * | 2003-09-09 | 2005-03-17 | Zephyr Proteomix Ltd. | Libraries of chimeric cellulose binding proteins and methods of use thereof |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH11225763A (ja) * | 1998-02-12 | 1999-08-24 | Toyobo Co Ltd | タンパク質の精製方法 |
ES2224792B1 (es) * | 2002-06-28 | 2007-02-16 | Era Plantech, S.L. | Produccion de peptidos y proteinas por acumulacion de cuerpos proteicos derivados de reticulos endoplasmico en plantas. |
KR100618563B1 (ko) * | 2003-10-29 | 2006-08-31 | 한국생명공학연구원 | 셀룰로오스 결합 도메인을 이용한 효모에서 재조합단백질의 제조방법 |
KR101161622B1 (ko) * | 2009-08-31 | 2012-07-04 | 헬릭스 주식회사 | 번역 효율 증진용 dna 단편 및 이를 포함하는 재조합 벡터 |
JP5641478B2 (ja) * | 2010-07-18 | 2014-12-17 | 独立行政法人国際農林水産業研究センター | 酵素の再利用方法 |
KR20130130563A (ko) * | 2012-05-22 | 2013-12-02 | (재) 스크립스코리아항체연구원 | 전체 세포 분리 방법 |
KR101507207B1 (ko) * | 2012-10-05 | 2015-03-31 | 주식회사 바이오앱 | 식물에서 셀룰로오즈와 셀룰로오즈 결합 도메인을 사용하여 단백질을 분리정제하는 방법 |
KR101848082B1 (ko) | 2017-05-11 | 2018-04-12 | 주식회사 바이오앱 | 셀룰로오즈 결합 도메인을 포함하는 재조합 벡터 및 상기 벡터를 사용하여 단백질을 분리정제하는 방법 |
-
2017
- 2017-05-11 KR KR1020170058882A patent/KR101848082B1/ko active IP Right Grant
-
2018
- 2018-05-10 US US16/611,966 patent/US10934558B2/en active Active
- 2018-05-10 CN CN201880031103.0A patent/CN110709518B/zh active Active
- 2018-05-10 EP EP18798939.7A patent/EP3623477A4/en active Pending
- 2018-05-10 JP JP2019561814A patent/JP6945883B2/ja active Active
- 2018-05-10 WO PCT/KR2018/005371 patent/WO2018208099A2/ko unknown
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005021762A2 (en) * | 2003-08-27 | 2005-03-10 | Orf Liftaekni Hf. | A process for proteolytic cleavage and purification of recombinant proteins produced in plants |
WO2005023981A2 (en) * | 2003-09-09 | 2005-03-17 | Zephyr Proteomix Ltd. | Libraries of chimeric cellulose binding proteins and methods of use thereof |
Non-Patent Citations (2)
Title |
---|
ERIN EGELKROUT ET AL: "Overproduction of recombinant proteins in plants", PLANT SCIENCE, ELSEVIER IRELAND LTD, IE, vol. 184, 9 December 2011 (2011-12-09), pages 83 - 101, XP028452706, ISSN: 0168-9452, [retrieved on 20111216], DOI: 10.1016/J.PLANTSCI.2011.12.005 * |
ULLRICH KRISTIAN K ET AL: "Means to optimize protein expression in transgenic plants", CURRENT OPINION IN BIOTECHNOLOGY, LONDON, GB, vol. 32, 25 November 2014 (2014-11-25), pages 61 - 67, XP029584739, ISSN: 0958-1669, DOI: 10.1016/J.COPBIO.2014.11.011 * |
Also Published As
Publication number | Publication date |
---|---|
US20200270624A1 (en) | 2020-08-27 |
KR101848082B1 (ko) | 2018-04-12 |
JP2020519264A (ja) | 2020-07-02 |
CN110709518A (zh) | 2020-01-17 |
EP3623477A2 (en) | 2020-03-18 |
JP6945883B2 (ja) | 2021-10-06 |
WO2018208099A3 (ko) | 2019-01-10 |
CN110709518B (zh) | 2023-08-15 |
WO2018208099A2 (ko) | 2018-11-15 |
US10934558B2 (en) | 2021-03-02 |
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