EP3386955B1 - Intermédiaires pour la production de sacubitril et leurs preparation - Google Patents

Intermédiaires pour la production de sacubitril et leurs preparation Download PDF

Info

Publication number
EP3386955B1
EP3386955B1 EP16810077.4A EP16810077A EP3386955B1 EP 3386955 B1 EP3386955 B1 EP 3386955B1 EP 16810077 A EP16810077 A EP 16810077A EP 3386955 B1 EP3386955 B1 EP 3386955B1
Authority
EP
European Patent Office
Prior art keywords
formula
alkyl
compound
salt
hydrogen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP16810077.4A
Other languages
German (de)
English (en)
Other versions
EP3386955A1 (fr
Inventor
Florian Karl KLEINBECK-RINIKER
Benjamin Martin
Gerhard Penn
Francesco VENTURONI
Thierry Schlama
Thomas Ruch
Thomas Allmendinger
Bernhard Wietfeld
Paolo Filipponi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Original Assignee
Novartis AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis AG filed Critical Novartis AG
Publication of EP3386955A1 publication Critical patent/EP3386955A1/fr
Application granted granted Critical
Publication of EP3386955B1 publication Critical patent/EP3386955B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C69/00—Esters of carboxylic acids; Esters of carbonic or haloformic acids
    • C07C69/66—Esters of carboxylic acids having esterified carboxylic groups bound to acyclic carbon atoms and having any of the groups OH, O—metal, —CHO, keto, ether, acyloxy, groups, groups, or in the acid moiety
    • C07C69/73—Esters of carboxylic acids having esterified carboxylic groups bound to acyclic carbon atoms and having any of the groups OH, O—metal, —CHO, keto, ether, acyloxy, groups, groups, or in the acid moiety of unsaturated acids
    • C07C69/738—Esters of keto-carboxylic acids or aldehydo-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C219/00—Compounds containing amino and esterified hydroxy groups bound to the same carbon skeleton
    • C07C219/02—Compounds containing amino and esterified hydroxy groups bound to the same carbon skeleton having esterified hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton
    • C07C219/20—Compounds containing amino and esterified hydroxy groups bound to the same carbon skeleton having esterified hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being unsaturated
    • C07C219/22—Compounds containing amino and esterified hydroxy groups bound to the same carbon skeleton having esterified hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being unsaturated and containing six-membered aromatic rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C213/00—Preparation of compounds containing amino and hydroxy, amino and etherified hydroxy or amino and esterified hydroxy groups bound to the same carbon skeleton
    • C07C213/08—Preparation of compounds containing amino and hydroxy, amino and etherified hydroxy or amino and esterified hydroxy groups bound to the same carbon skeleton by reactions not involving the formation of amino groups, hydroxy groups or etherified or esterified hydroxy groups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C229/00—Compounds containing amino and carboxyl groups bound to the same carbon skeleton
    • C07C229/02—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton
    • C07C229/34—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton containing six-membered aromatic rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C251/00—Compounds containing nitrogen atoms doubly-bound to a carbon skeleton
    • C07C251/32—Oximes
    • C07C251/34—Oximes with oxygen atoms of oxyimino groups bound to hydrogen atoms or to carbon atoms of unsubstituted hydrocarbon radicals
    • C07C251/36—Oximes with oxygen atoms of oxyimino groups bound to hydrogen atoms or to carbon atoms of unsubstituted hydrocarbon radicals with the carbon atoms of the oxyimino groups bound to hydrogen atoms or to acyclic carbon atoms
    • C07C251/40—Oximes with oxygen atoms of oxyimino groups bound to hydrogen atoms or to carbon atoms of unsubstituted hydrocarbon radicals with the carbon atoms of the oxyimino groups bound to hydrogen atoms or to acyclic carbon atoms to carbon atoms of an unsaturated carbon skeleton
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C251/00—Compounds containing nitrogen atoms doubly-bound to a carbon skeleton
    • C07C251/32—Oximes
    • C07C251/62—Oximes having oxygen atoms of oxyimino groups esterified
    • C07C251/64—Oximes having oxygen atoms of oxyimino groups esterified by carboxylic acids
    • C07C251/66—Oximes having oxygen atoms of oxyimino groups esterified by carboxylic acids with the esterifying carboxyl groups bound to hydrogen atoms, to acyclic carbon atoms or to carbon atoms of rings other than six-membered aromatic rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C313/00—Sulfinic acids; Sulfenic acids; Halides, esters or anhydrides thereof; Amides of sulfinic or sulfenic acids, i.e. compounds having singly-bound oxygen atoms of sulfinic or sulfenic groups replaced by nitrogen atoms, not being part of nitro or nitroso groups
    • C07C313/02—Sulfinic acids; Derivatives thereof
    • C07C313/06—Sulfinamides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
    • C07C51/347—Preparation of carboxylic acids or their salts, halides or anhydrides by reactions not involving formation of carboxyl groups
    • C07C51/373—Preparation of carboxylic acids or their salts, halides or anhydrides by reactions not involving formation of carboxyl groups by introduction of functional groups containing oxygen only in doubly bound form
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C57/00—Unsaturated compounds having carboxyl groups bound to acyclic carbon atoms
    • C07C57/46—Unsaturated compounds having carboxyl groups bound to acyclic carbon atoms containing six-membered aromatic rings and other rings, e.g. cyclohexylphenylacetic acid
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C59/00—Compounds having carboxyl groups bound to acyclic carbon atoms and containing any of the groups OH, O—metal, —CHO, keto, ether, groups, groups, or groups
    • C07C59/40—Unsaturated compounds
    • C07C59/76—Unsaturated compounds containing keto groups
    • C07C59/84—Unsaturated compounds containing keto groups containing six membered aromatic rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C67/00—Preparation of carboxylic acid esters
    • C07C67/035—Preparation of carboxylic acid esters by reacting carboxylic acids or symmetrical anhydrides with saturated hydrocarbons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C67/00—Preparation of carboxylic acid esters
    • C07C67/08—Preparation of carboxylic acid esters by reacting carboxylic acids or symmetrical anhydrides with the hydroxy or O-metal group of organic compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D207/00—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D207/02—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D207/30—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having two double bonds between ring members or between ring members and non-ring members
    • C07D207/34—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having two double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D207/36—Oxygen or sulfur atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D265/00—Heterocyclic compounds containing six-membered rings having one nitrogen atom and one oxygen atom as the only ring hetero atoms
    • C07D265/02—1,2-Oxazines; Hydrogenated 1,2-oxazines
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D267/00—Heterocyclic compounds containing rings of more than six members having one nitrogen atom and one oxygen atom as the only ring hetero atoms
    • C07D267/02—Seven-membered rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/07—Optical isomers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Definitions

  • the present invention relates to a new chemical synthesis route and intermediates useful for the preparation of neprilysin (NEP) inhibitors and their prodrugs, in particular for the NEP inhibitor prodrug sacubitril.
  • NEP neprilysin
  • NEP inhibitor prodrug sacubitril N -(3-carboxyl-1-oxopropyl)-(4 S )-(p-phenylphenyl-methyl)-4-amino-(2 R )-methyl butanoic acid ethyl ester; IUPAC name 4- ⁇ [(1S,3 R )-1-([1,1'-biphenyl]-4-ylmethyl)-4-ethoxy-3-methyl-4-oxobutyl]amino ⁇ -4-oxobutanoic acid, also known as AHU377) is represented by the following formula (A)
  • Sacubitril together with valsartan a known angiotensin receptor blocker (ARB), forms a sodium salt hydrate complex, known as LCZ696, comprising the anionic forms of sacubitril and valsartan, sodium cations and water molecules in the molar ratio of 1:1:3:2.5, respectively (ratio of 6:6:18:15 in the asymmetric unit cell of the solid state crystal), and which is schematically present in formula (B).
  • ARB angiotensin receptor blocker
  • Said complex is also referred to by the following chemical names: Trisodium [3-((1 S ,3 R )-1-biphenyl-4-ylmethyl-3-ethoxycarbonyl-1-butylcarbamoyl)propionate-(S)-3'-methyl-2'-(pentanoyl ⁇ 2"-(tetrazol-5-ylate)biphenyl-4'-ylmethyl ⁇ amino)butyrate] hemipentahydrate or Octadecasodium hexakis(4- ⁇ [(1S,3R)-1-([1,1'-biphenyl]-4-ylmethyl)-4-ethoxy-3-methyl-4-oxobutyl]amino ⁇ -4-oxobutanoate) hexakis( N -pentanoyl- N - ⁇ [2'-(1 H -tetrazol-1-id-5-yl)[1,1'-biphenyl]-4
  • LCZ696 acts as angiotensin receptor neprilysin inhibitor (ARNI) and is therefore useful particularly in the treatment of hypertention or chronic heart failure. Its utility has been confirmed by clinical trials, e.g. in the landmark PARADIGM-HF trial.
  • ARNI angiotensin receptor neprilysin inhibitor
  • the invention relates to novel intermediates and process steps and processes for the manufacture of a compound of formula (XV), especially (XV-a) represented below, and its further use in the manufacture of sacubitril.
  • the present invention provides the following new compounds:
  • the compound of the formula (XV) is represented by formula (XV-a) with the following stereochemistry wherein
  • the compound of formula (XV) is a compound of formula (I), or a salt thereof; wherein Ra is selected from hydrogen, a carboxyl protecting group, and C 1 -C 6 -alkyl, preferably ethyl wherein the carboxyl protecting group is selected from C6-C10-aryl-C1-C6-alkyl and SiR11 R12R13, wherein R11, R12, and R13 are, independently of each other, C1 -C7-alkyl,C6-C10-aryl or phenyl-C1-C4-alkyl.
  • Ra is selected from hydrogen, a carboxyl protecting group, and C 1 -C 6 -alkyl, preferably ethyl wherein the carboxyl protecting group is selected from C6-C10-aryl-C1-C6-alkyl and SiR11 R12R13, wherein R11, R12, and R13 are, independently of each other, C1 -C7-alkyl,C
  • the compound of the formula (I) is represented by formula (I-a) with the following stereochemistry wherein Ra is selected from hydrogen, a carboxyl protecting group, and C 1 -C 6 -alkyl, preferably ethyl.
  • the compound of formula (XV) is a compound of formula (II), or a salt thereof; wherein Ra and R* are, independently of each other, selected from hydrogen, a carboxyl protecting group, and C 1 -C 6 -alkyl, preferably ethyl wherein the carboxyl protecting group is selected from C6-C10-aryl-C1-C6-alkyl and SiR11R12R13, wherein R11, R12, and R13 are, independently of each other, C1-C7-alkyl,C6-C10-aryl or phenyl-C1-C4-alkyl.
  • the compound of the formula (II) is represented by formula (II-a) with the following stereochemistry wherein Ra and R* are, independently of each other, selected from hydrogen, a carboxyl protecting group, and C 1 -C 6 -alkyl, preferably ethyl wherein the carboxyl protecting group is selected from C6-C10-aryl-C1-C6-alkyl and SiR11R12R13, wherein R11, R12, and R13 are, independently of each other, C1-C7-alkyl,C6-C10-aryl or phenyl-C1-C4-alkyl.
  • the compound of the formula (XVI) is represented by formula (XVI-a) with the following stereochemistry wherein
  • the compound of formula (XVI) is a compound of formula (X), or a salt thereof
  • the compound of the formula (X) is represented by formula (X-a) with the following stereochemistry wherein Ra is selected from hydrogen, a carboxyl protecting group, wherein the carboxyl protecting group is selected from C6-C10-aryl-C1-C6-alkyl and SiR11 R12R13, wherein R11, R12, and R13 are, independently of each other, C1 -C7-alkyl,C6-C10-aryl or phenyl-C1-C4-alkyl, and C 1 -C 6 -alkyl, preferably ethyl, and Rb is selected from C 1 -C 6 -alkyl, C 6 -C 10 -aryl, C 6 -C 10 -aryl-C 1 -C 6 -alkyl, C 3 -C 8 -cycloalkyl, C 3 -C 8 -cycloalkyl-C 1 -C 6 -alkyl, heterocyclyl or heterocycly
  • the compound of formula (XVI) is a compound of formula (XVII), or a salt thereof wherein Ra is selected from hydrogen, a carboxyl protecting group, wherein the carboxyl protecting group is selected from C6-C10-aryl-C1-C6-alkyl and SiR11 R12R13, wherein R11, R12, and R13 are, independently of each other, C1 -C7-alkyl,C6-C10-aryl or phenyl-C1-C4-alkyl, and C 1 -C 6 -alkyl, preferably ethyl.
  • Ra is selected from hydrogen, a carboxyl protecting group, wherein the carboxyl protecting group is selected from C6-C10-aryl-C1-C6-alkyl and SiR11 R12R13, wherein R11, R12, and R13 are, independently of each other, C1 -C7-alkyl,C6-C10-aryl or phenyl-C1-C4
  • the compound of the formula (XVII) is represented by formula (XVII-a) with the following stereochemistry wherein Ra is selected from hydrogen, a carboxyl protecting group, and C 1 -C 6 -alkyl, preferably ethyl, wherein the carboxyl protecting group is selected from C6-C10-aryl-C1-C6-alkyl and SiR11 R12R13, wherein R11, R12, and R13 are, independently of each other, C1-C7-alkyl,C6-C10-aryl or phenyl-C1-C4-alkyl.
  • Ra is selected from hydrogen, a carboxyl protecting group, and C 1 -C 6 -alkyl, preferably ethyl
  • the carboxyl protecting group is selected from C6-C10-aryl-C1-C6-alkyl and SiR11 R12R13, wherein R11, R12, and R13 are, independently of each other, C1-C7-alkyl
  • the compound of formula (XVI) is a compound of formula (XVII*), or a salt thereof wherein Ra is selected from hydrogen, a carboxyl protecting group, and C 1 -C 6 -alkyl, preferably ethyl, wherein the carboxyl protecting group is selected from C6-C10-aryl-C1-C6-alkyl and SiR11R12R13, wherein R11, R12, and R13 are, independently of each other, C1-C7-alkyl,C6-C10-aryl or phenyl-C1-C4-alkyl, and Rc is selected from C 1 -C 6 -alkyl, C 6 -C 10 -aryl, C 6 -C 10 -aryl-C 1 -C 6 -alkyl, C 3 -C 8 -cycloalkyl, C 3 -C 8 -cycloalkyl-C 1 -C 6 -alkyl, heterocyclyl or hetero
  • the compound of the formula (XVII*) is represented by formula (XVII*-a) with the following stereochemistry wherein Ra is selected from hydrogen, a carboxyl protecting group, and C 1 -C 6 -alkyl, preferably ethyl, wherein the carboxyl protecting group is selected from C6-C10-aryl-C1-C6-alkyl and SiR11R12R13, wherein R11, R12, and R13 are, independently of each other, C1 -C7-alkyl,C6-C10-aryl or phenyl-C1-C4-alkyl, and Rc is selected from C 1 -C 6 -alkyl, C 6 -C 10 -aryl, C 6 -C 10 -aryl-C 1 -C 6 -alkyl, C 3 -C 8 -cycloalkyl, C 3 -C 8 -cycloalkyl-C 1 -C 6 -alkyl, heterocycl
  • the compound of formula (XVI) is a compound of formula (XVII**), or a salt thereof wherein Ra is selected from hydrogen, a carboxyl protecting group, and C 1 -C 6 -alkyl, preferably ethyl, wherein the carboxyl protecting group is selected from C6-C10-aryl-C1-C6-alkyl and SiR11R12R13, wherein R11, R12, and R13 are, independently of each other, C1-C7-alkyl,C6-C10-aryl or phenyl-C1-C4-alkyl, and Rd is selected from C 1 -C 6 -alkyl, C 6 -C 10 -aryl, C 6 -C 10 -aryl-C 1 -C 6 -alkyl, C 3 -C 8 -cycloalkyl, C 3 -C 8 -cycloalkyl-C 1 -C 6 -alkyl, heterocyclyl or hetero
  • the compound of the formula (XVII**) is represented by formula (XVII**-a) with the following stereochemistry wherein Ra is selected from hydrogen, a carboxyl protecting group, and C 1 -C 6 -alkyl, preferably ethyl, wherein the carboxyl protecting group is selected from C6-C10-aryl-C1-C6-alkyl and SiR11R12R13, wherein R11, R12, and R13 are, independently of each other, C1-C7-alkyl,C6-C10-aryl or phenyl-C1-C4-alkyl, and Rd is selected from C 1 -C 6 -alkyl, C 6 -C 10 -aryl, C 6 -C 10 -aryl-C 1 -C 6 -alkyl, C 3 -C 8 -cycloalkyl, C 3 -C 8 -cycloalkyl-C 1 -C 6 -alkyl, heterocycly
  • the compound of formula (XVI) is a compound of formula (XVIII), or a salt thereof
  • the compound of the formula (XVIII) is represented by formula (XVIII-a) with the following stereochemistry
  • the compound of formula (XVI) is a compound of formula (XIX), or a salt thereof
  • the compound of the formula (XIXI) is represented by formula (XIX-a) with the following stereochemistry
  • the present invention provides a new process for the manufacture of the novel compound of formula (I), in particular of formula (I-a), or a salt thereof, as defined herein.
  • This process comprises several steps via novel intermediate compounds and is depicted in the following Schemes 1 to 4, respectively, wherein each process depicted in the corresponding SCHEMEs 1, 2, 3, or 3* represents a separate embodiment of the invention.
  • SCHEME 1 and SCHEME 1-a depict the process comprising reacting the novel intermediate compound of formula (II), preferably of formula (II-a), or a salt thereof, wherein Ra and R* are, independently of each other, selected from hydrogen, a carboxyl protecting group, and C 1 -C 6 -alkyl, preferably ethyl, under - if required - deprotection reaction conditions followed by decarboxylation reaction conditions, and optionally - if necessary or desired, e.g.
  • Ra is cleaved off during deprotection - by introduction of a moiety Ra selected from a carboxyl protecting group and C 1 -C 6 -alkyl, to provide the compound of formula (I) preferably of formula (I-a), or a salt thereof, wherein Ra is selected from hydrogen, a carboxyl protecting group and C 1 -C 6 -alkyl, preferably ethyl.
  • R* and Ra are different and Ra is already C 1 -C 6 -alkyl, especially ethyl, then only R* (e.g. tert-butyl) can be cleaved off selectively, while under the same conditions the C 1 -C 6 -alkyl, e.g. ethyl, remains bound.
  • R* and Ra are different and Ra is already C 1 -C 6 -alkyl, especially ethyl, then only R* (e.g. tert-butyl) can be cleaved off selectively
  • the compound of formula (II) can be obtained according to a reaction as depicted in the following SCHEMES 2 and 2-a:
  • SCHEME 2 and SCHEME 2-a both depict a process comprising reacting a compound of formula (III), with a propionate derivative of formula (IV), preferably of formula (IV-a), wherein X is a leaving group and Ra is selected from a carboxyl protecting group and C 1 -C 6 -alkyl, preferably ethyl, to obtain a compound of formula (II), preferably of formula (II-a), wherein Ra and R* are, independently of each other, selected from hydrogen, a carboxyl protecting group, and C 1 -C 6 -alkyl, preferably ethyl.
  • this can include replacing the carboxyl protecting groups R* and Ra with a group selected from hydrogen and C 1 -C 6 -alkyl, to provide the compound of formula (II).
  • the compound of formula (III) can be obtained according to a reaction as depicted in the following SCHEME 3:
  • SCHEME 3 depicts a process comprising reacting a compound of formula (V), or a reactive derivative thereof, with a salt of a malonic acid half ester of formula (VI), wherein R* is selected from a carboxyl protecting group and C 1 -C 6 -alkyl, preferably ethyl, to obtain a compound of formula (III).
  • novel compound of formula (I), in particular of formula (I-a), or a salt thereof can also be obtained by a process as depicted in the following Scheme 3* or 3*-a, which also represents a separate embodiment of the invention.
  • SCHEME 3* and SCHEME 3*-a depict a process comprising reacting the intermediate compound of formula (XIV), preferably of formula (XIV-a), or a salt thereof, wherein Rf is selected from a group -O-R* wherein R* is selected from a carboxyl protecting group and C 1 -C 6 -alkyl, preferably methyl; -N(CH3)-O(CH3), morpholinyl, and imidazolinyl, with an in situ prepared activated dianionic derivative of the compound of formula (V), or a salt thereof, namely a compound of formula (V*), in the presence of a base, and followed by a decarboxylation reaction, to obtain the compound of formula (I), or a salt thereof wherein Ra is hydrogen.
  • Such compound of formula XIV is known in the art. If necessary or desired, the obtained compound of formula (I), or a salt thereof, wherein Ra is hydrogen, can be reacted with an agent introducing a carboxyl protecting group, to provide the compound of formula (I), wherein Ra is a carboxyl protecting group, or the obtained compound of formula (I), or a salt thereof, wherein Ra is hydrogen, is reacted with a coupling reagent in the presence of an C 1 -C 6 -alkanol, especially ethanol, to provide the compound of formula (I), wherein Ra is C 1 -C 6 -alkyl, preferably ethyl.
  • the compound of formula (V) as used as starting compound in SCHEMEs 3 and 3* can be obtained according to a reaction as depicted in the following SCHEME 4:
  • SCHEME 4 depicts a process comprising hydrolysing a cyanide compound of formula (VII) to obtain a compound of formula (V).
  • reaction sequence starts from SCHEME 3: SCHEME 3 -> SCHEME 2 -> SCHEME 1.
  • reaction sequence starts from SCHEME 2: SCHEME 2 -> SCHEME 1.
  • the present invention provides a new process for the manufacture of a compound of the formula (VIII), in particular of formula (VIII-a), more preferably of formula (VIII-aa), or a salt thereof, as depicted below and as defined herein, by various reactions all starting from the novel compound of formula (I), in particular of formula (I-a), or a salt thereof, as defined herein.
  • the overall reaction is depicted in the following SCHEMEs 5, 5-a and 5-aa:
  • the novel compound of formula (I) can be converted into a compound of formula (VIII) according to a reaction (“Reaction with ammonia, a primary or a secondary amine, or salts thereof") as depicted in SCHEMES 5, 5-A and 5-aa above (therefore not shown separately).
  • SCHEMEs 5, 5-A and 5-aa depict the process comprising reacting the novel intermediate compound of formula (I), preferably of formula (I-a), or a salt thereof, wherein Ra is hydrogen, with ammonia, a primary or secondary amine, or salts thereof, and optionally cleaving off any undesired substituents of the amine, to provide a compound of formula (VIII), in particular of formula (VIII-a), more preferably of formula (VIII-aa), or a salt thereof, wherein Ra and Re are hydrogen, or if the primary amine has the formula NH 2 -Re, with Re being a nitrogen protecting group, to provide a compound of formula (VIII), in particular of formula (VIII-a), more preferably of formula (VIII-aa), or a salt thereof, wherein Ra is hydrogen and Re is a nitrogen protecting group, and optionally reacting the obtained compound of formula (VIII), in particular of formula (VIII-a), more preferably of formula (VIII-aa), or
  • the compound of formula (I) is obtained by a process as depicted in SCHEME 1, optionally including the reaction steps as depicted in SCHEMEs 2 to 4, or by a process as depicted in SCHEME 3*, optionally including the reaction steps as depicted in SCHEME 4.
  • novel compound of formula (I) can be converted into a compound of formula (VIII) according to a reaction as depicted in the following SCHEMES 6, 6-1, 6-A and 6-aa ("Reaction using a transaminase"):
  • SCHEMEs 6, 6-1, 6-A and 6-aa (“Reaction using a transaminase”) depict the process comprising converting the novel intermediate compound of formula (I) or formula (I-a), or a salt thereof, wherein Ra is selected from hydrogen and C 1 -C 6 -alkyl, preferably hydrogen, into the compound of formula (VIII), preferably of formula (VIII-1), or of formula (VIII-a), more preferably of formula (VIII-aa), wherein Ra is selected from hydrogen and C 1 -C 6 -alkyl, preferably hydrogen, and Re is hydrogen, or a salt thereof, by bringing it in contact with an (S)-selective ⁇ -transaminase in the presence of an amine donor of the general formula R'R"CH-NH 2 and a coenzyme, wherein the conversion rate from the compound of formula (I) to the compound of formula (VIII), preferably of formula (VIII-1), in particular the conversion rate from the compound of formula (I-a) to
  • the optionally amino protected analog of the compound of the formula (VIII), in particular of formula (VIII-1), or of formula (VIII-a), preferably of formula (VIII-aa), wherein Ra is hydrogen is optionally reacted with a coupling reagent in the presence of an C 1 -C 6 -alkanol, especially ethanol, to provide the compound of formula (VIII), in particular of formula (VIII-1), or of formula (VIII-a), preferably of formula (VIII-aa), or a salt thereof, wherein Ra is C 1 -C 6 -alkyl, preferably ethyl, and Re is hydrogen or a nitrogen protecting group.
  • the compound of formula (I) is obtained by a process as depicted in SCHEME 1, optionally including the reaction steps as depicted in SCHEMEs 2, 2 to 3, or 2 to 4, or by a process as depicted in SCHEME 3*, optionally including the reaction steps as depicted in SCHEME 4.
  • novel compound of formula (I) can be converted into a compound of formula (VIII) according to a reaction as depicted in the following SCHEMES 7, 7-a and 7-aa ("reaction via the novel sulfinimide compound of the formula (X)"):
  • SCHEMEs 7, 7-A and 7-aa depict the process comprising reacting the novel intermediate compound of formula (I), preferably of formula (I-a), or a salt thereof, wherein Ra is selected from a carboxyl protecting group and C 1 -C 6 -alkyl, preferably ethyl, with an aminosulfinyl compound of formula (IX), especially of formula (IX-a), or a salt thereof, wherein Rb is selected from C 1 -C 6 -alkyl, C 6 -C 10 -aryl, C 6 -C 10 -aryl-C 1 -C 6 -alkyl, C 3 -C 8 -cycloalkyl, C 3 -C 8 -cycloalkyl-C 1 -C 6 -alkyl, heterocyclyl or heterocyclyl-C 1 -C 6 -alkyl, wherein said heterocyclyl is a mono- or polycyclic, unsaturated, partially saturated, saturated or aromatic ring system
  • the compound of formula (I) is obtained by a process as depicted in SCHEME 1, optionally including the reaction steps as depicted in SCHEMEs 2 to 4, or by a process as depicted in SCHEME 3*, optionally including the reaction steps as depicted in SCHEME 4.
  • novel compound of formula (I) can be converted into a compound of formula (VIII) according to a reaction as depicted in the following SCHEMES 8, 8-a and 8-aa ("reaction with hydroxylamine via the novel compounds of formula (XVII) and of formula (XVIII)"):
  • SCHEMEs 8, 8-A and 8-AA depict the process comprising reacting the novel intermediate compound of formula (I), preferably of formula (I-a), or a salt thereof, wherein Ra is hydrogen, with hydroxylamine or a salt thereof, to provide a compound of formula (XVII), preferably of formula (XVII-a), or a salt thereof, wherein Ra is hydrogen, subsequently cyclizing the obtained compound of formula (XVII), preferably of formula (XVII-a), or a salt thereof, to give the corresponding compound of the formula (XVIII), preferably of formula (XVIII-a), or a salt thereof, then reducing the obtained compound of formula (XVIII), preferably of formula (XVIII-a), or a salt thereof, in the presence of a reducing agent, to obtain the compound of formula (VIII), preferably of formula (VIII-a), more preferably of formula (VIII-aa), or a salt thereof, wherein Ra and Re are both hydrogen, and optionally reacting the obtained compound of
  • the compound of formula (I) is obtained by a process as depicted in SCHEME 1, optionally including the reaction steps as depicted in SCHEMEs 2 to 4, or by a process as depicted in SCHEME 3*, optionally including the reaction steps as depicted in SCHEME 4.
  • novel compound of formula (I) can be converted into a compound of formula (VIII) according to a reaction as depicted in the following SCHEMES 9, 9-a and 9-aa ("reaction with ammonia or an ammonium via the novel compounds of formula (XIX) and (XX):
  • SCHEMEs 9, 9-A and 9-AA depict the process comprising reacting the novel intermediate compound of formula (I), preferably of formula (I-a), or a salt thereof, wherein Ra is hydrogen, with ammonia or an ammonium salt, yielding a compound of the formula (XIX), preferably of formula (XIX-a), or a salt thereof, reducing the obtained compound of formula (XIX), especially (XIX-a), with a reducing agent, to give the lactam compound of formula (XX), preferably of formula (XX-a), more preferably of formula (XX-aa), or a salt thereof, and reacting the obtained compound of formula (XX) under ring opening conditions, optionally in the presence of an C 1 -C 7 -alcohol, to provide the compound of formula (VIII), preferably of formula (VIII-a), more preferably of formula (VIII-aa), or a salt thereof, wherein Ra is selected from hydrogen and C 1 -C 6 -alkyl,
  • the compound of formula (I) is obtained by a process as depicted in SCHEME 1, optionally including the reaction steps as depicted in SCHEMEs 2 to 4, or by a process as depicted in SCHEME 3*, optionally including the reaction steps as depicted in SCHEME 4.
  • the novel compound of formula (I) can be converted into a compound of formula (VIII) according to a reaction as depicted in the following SCHEMES 10, 10-a and 10-aa ("reaction with an O-substituted hydroxylamine via the novel compounds of formula (XVII***), especially of formula (XVII*) and of formula (XVII**)"):
  • SCHEMEs 10, 10-A and 10-AA depict the process comprising reacting the novel intermediate compound of formula (I), preferably of formula (I-a), or a salt thereof, wherein Ra is selected from hydrogen, a carboxyl protecting group and C 1 -C 6 -alkyl, preferably ethyl, with a O-substituted hydroxylamine selected from or in each case a salt thereof, wherein Rc and Rd are independently selected from C 1 -C 6 -alkyl, C 6 -C 10 -aryl, C 6 -C 10 -aryl-C 1 -C 6 -alkyl, C 3 -C 8 -cycloalkyl, C 3 -C 8 -cycloalkyl-C 1 -C 6 -alkyl, heterocyclyl or heterocyclyl-C 1 -C 6 -alkyl, wherein said heterocyclyl is a mono- or polycyclic, unsaturated, partially saturated, saturated or aromatic ring system with 5 to
  • the compound of formula (I) is obtained by a process as depicted in SCHEME 1, optionally including the reaction steps as depicted in SCHEMEs 2 to 4, or by a process as depicted in SCHEME 3*, optionally including the reaction steps as depicted in SCHEME 4.
  • the process as depicted in SCHEMEs 5 to 10 is continued by transforming the obtained compound of formula (VIII) or a salt thereof preferably of formula (VIII-a), or a salt thereof more preferably of formula (VIII-aa), or a salt thereof wherein Ra is selected from hydrogen and C 1 -C 6 -alkyl, preferably ethyl, and Re is selected from hydrogen and a nitrogen protecting group, into a compound of formula (10) preferably of formula (10-a) wherein R1 is hydrogen or C 1 -C 6 -alkyl, preferably ethyl, in particular to the compound N-(3-carboxy-1-oxopropyl)-(4S)-p-phenylphenylmethyl)-4-amino-(2R)-methylbutanoic acid or a salt thereof, or N -(3-carboxy-1-oxopropyl)-(4 S )-p-phenylphenylmethyl)-4-a
  • the present invention provides the use of a novel compound of formula (XV), in particular of formula (I) or (II), of a novel compound of formula (XVI), in particular of formula (X), (XVII), (XVII*), (XVII**), (XVIII), and (IX), or the preferred stereoisomer thereof, as depicted above, in the manufacture of a compound of formula (10) preferably of formula (10-a) wherein R1 is hydrogen or C 1 -C 6 -alkyl, preferably ethyl, preferably in the manufacture of N -(3-carboxyl-1-oxopropyl)-(4S)-( p -phenylphenylmethyl)-4-amino-(2R)-methyl butanoic acid, or salts thereof, or N -(3-carboxyl-1-oxopropyl)-(4 S )-( p- phenylphenylmethyl)-4-amino-(
  • the invention relates to any one or more of the novel compounds, processes and uses as represented in the claims, which are incorporated here by reference.
  • the invention also relates to any sequential combination of the process steps described above and below.
  • the synthesis route is suitable for industrial scale processing.
  • the synthesis route is economically and environmentally favorable.
  • the compounds of formula (I) and (XVI) which are intermediates desired for the synthesis of sacubitril can be produced with high yield and high stereo-selectivity.
  • chiral refers to molecules which have the property of non-superimposability on their mirror image partner, while the term “achiral” refers to molecules which are superimposable on their mirror image partner.
  • the term " on a C-sp 3 represents a covalent bond, wherein the stereochemistry of the bond is not defined.
  • the term " " on a C-sp 3 comprises an (S) configuration as well as an (R) configuration of the respective chiral centre.
  • mixtures e.g. mixtures of enantiomers such as racemates, are also encompassed by the present invention.
  • the term " on a C-sp 2 represents a covalent bond, wherein the stereochemistry or the geometry of the bond is not defined. This means that the term “ " " on a C-Sp 2 comprises a (Z) configuration as well as a (E) configuration of the respective double bond. Furthermore, mixtures, e.g., mixtures of double bond isomers are also encompassed by the present invention.
  • the compounds of the present invention can possess one or more asymmetric centers.
  • the preferred absolute configurations are as indicated herein specifically. However, any possible pure enantiomer, pure diastereoisomer, or mixtures thereof, e.g., mixtures of enantiomers, such as racemates, are encompassed by the present invention.
  • Stereoisomeric, especially enantiomeric, purity is where mentioned referring to all diastereomers of the compound taken together (100%). It is determined by chiral chromatography (examples include HPLC, uPLC and GC) or NMR (with addition of chiral entities and or metals). Specific examples of methods include: chiral HPLC equipped with chiral column Chiralpak ID 4.6mm ⁇ x 250mm, 5 ⁇ m (Daicel Corporation, Osaka, Japan) at 25°C; mobile phase Hept:EtOAc:CH 3 CN, 90:8:2.
  • the optical purity is given in % excess of one enantiomer over the other enantiomer.
  • pro-drug represents in particular compounds which are transformed in vivo to the parent compound, for example, by hydrolysis in blood, for example as described in T. Higuchi and V. Stella, "Pro-drugs as Novel Delivery Systems", volume 14 of the ACS Symposium Series; Edward B. Roche, editor, “Bioreversible Carriers in Drug Design", American Pharmaceutical Association and Pergamon Press, 1987 ; H Bundgaard, editor, “Design of Prodrugs”, Elsevier, 1985 ; Judkins et al. Synthetic Communications 1996, 26, 4351-4367 , and " The Organic Chemistry of Drug Design and Drug Action", second edition, R. B. Silverman (particularly chapter 8, pages 497-557), Elsevier Academic Press, 2004 .
  • Pro-drugs therefore include drugs having a functional group which has been transformed into a reversible derivative thereof. Typically, such prodrugs are transformed to the active drug by hydrolysis.
  • Functional Group Reversible derivative Carboxylic acid Esters, including e.g. alkyl esters Alcohol Esters, including e.g. sulfates and phosphates as well as carboxylic acid esters Amine Amides, carbamates, imines, enamines, Carbonyl (aldehyde, Imines, oximes, acetals/ketals, enol esters, ketone) oxazolidines and thiazoxolidines
  • Pro-drugs also include compounds convertible to the active drug by an oxidative or reductive reaction. As examples may be mentioned:
  • NEP inhibitor describes a compound which inhibits the activity of the enzyme neutral endopeptidase (NEP, EC 3.4.24.11).
  • NEP-inhibitor or “NEP-inhibitor prodrug” relates to the substances as such or to salts thereof, preferably pharmaceutically acceptable salts thereof.
  • Examples are sodium, potassium, magnesium, calcium or ammonium salts. Calcium salts are preferred.
  • NEP inhibitor prodrug N -(3-carboxy-1-oxopropyl)-(4 S )-p-phenylphenylmethyl)-4-amino-(2R)-methylbutanoic acid ethyl ester optionally may be further reacted to obtain the active NEP inhibitor N -(3-carboxy-1-oxopropyl)-(4 S )- p -phenylphenylmethyl)-4-amino-(2 R )-methylbutanoic acid, either in vitro or in vivo.
  • Transaminases are classified in E.C. 2.6.1.X.
  • the transaminase is an (R)- or (S)-selective transaminase, particularly is in a preferred embodiment an ⁇ -transaminase, in particular a (S)-selective ⁇ -transaminase.
  • a ⁇ -transaminase is an enzyme preferably with the classification code E.C.2.6.1.18. These amino transaminases are characterised in that they mainly use amines as substrates. These enzymes are further characterised by exhibiting an equilibrium constant of ⁇ -transaminase catalysed reactions which is greater than 1.
  • transaminase in particular ⁇ -transaminase
  • an extract of an organism such as a microorganism or a cell, containing a transaminase, in particular an ⁇ -transaminase, or a living or dead cell or microorganism itself comprising a transaminase, in particular an ⁇ -transaminase.
  • a microorganism or cell or extract or transaminase enzyme may be used in immobilised or non-immobilised form.
  • the transaminase in particular the ⁇ -transaminase, may also be a recombinantly produced naturally occurring (wild-type) or genetically modified transaminase, in particular an ⁇ -transaminase, which is coded partially or completely by a nucleic acid sequence or a derivative thereof contained in one of the above-identified organisms or being equivalent thereto.
  • transaminases which may be used and/or optimized to be used according to the present invention are described for instance in Koszelewski et al. (2010) Trends in Biotechnology, 28(6):324-332 , and Malik at al (2012) Appl Microbiol Biotechnol.;94(5):1163-71 .
  • Such transaminases can be obtained e.g. from microorganisms like Chromobacterium violaceum, Vibrio fluvialis, Alcaligenes denitrificans, Klebsiella pneumoniae, and Bacillus thuringiensis and others.
  • the ⁇ -transaminases used in the present invention were obtained from Codexis Inc. under the reference numbers ATA-217 (part of the Codex® ATA Screening Kit), as well as further genetically modified ⁇ -transaminases variants thereof (also obtained from Codexis Inc.). Such genetically modified ⁇ -transaminases are described e.g. in US patent No 8,470,564 .
  • PBP Pyridoxal-5'-phosphate
  • pyridoxal phosphate is defined by the structure 1-(4'-formyl-3'-hydroxy-2'-methyl-5'-pyridyl)methoxyphosphonic acid, CAS number [54-47-7 ], Pyridoxal-5'-phosphate can be produced in vivo by phosphorylation and oxidation of pyridoxol (also known as Vitamin B6).
  • pyridoxol also known as Vitamin B6
  • transamination reactions using transaminase enzymes the amine group of the amino donor is transferred to the coenzyme to produce a keto byproduct, while pyridoxal-5'-phosphate is converted to pyridoxamine phosphate.
  • Pyridoxal-5'-phosphate is regenerated by reaction with a different keto compound (the amino acceptor). The transfer of the amine group from pyridoxamine phosphate to the amino acceptor produces a chiral amine and regenerates the coenzyme.
  • the pyridoxal-5'-phosphate can be replaced by other members of the vitamin B6 family, including pyridoxine (PN), pyridoxal (PL), pyridoxamine (PM), and their phosphorylated counterparts; pyridoxine phosphate (PNP), and pyridoxamine phosphate (PMP).
  • PN pyridoxine
  • PL pyridoxal
  • PM pyridoxamine
  • PNP pyridoxine phosphate
  • PMP pyridoxamine phosphate
  • an amine donor is a molecule capable of providing an amino group to an amine acceptor using a transaminase, in particular an ⁇ -transaminase.
  • the amine donor is an amine or amino acid.
  • amino donors are molecules of the following general formula, in which each of R' and R", when taken independently, is an alkyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl, which is unsubstituted or substituted with one or more enzymatically non-inhibiting groups.
  • R' can be the same or different from R" in structure or chirality.
  • R' and R", taken together, may form a ring that is unsubstituted, substituted, or fused to other rings.
  • Typical amino donors that can be used include chiral and achiral amino acids, and chiral and achiral amines.
  • Amino donors that can be used include, by way of example and not limitation, isopropylamine (2-aminopropane), ⁇ -alanine, alanine, in particular D,L-alanine, L-alanine or D-alanine, ⁇ -methylbenzylamine (a-MBA), glutamate, phenylalanine, glycin, 3-aminobutyrate, 2-aminobutane, ⁇ -aminobutyrate and a salt, for instance a chloride, of any one thereof.
  • isopropylamine (2-aminopropane) is the amine donor.
  • the obtained ketone product will be acetone, phenylpyruvic acid, a salt thereof, pyruvic acid, a salt thereof, glyoxylic acid, a salt thereof, acetophenone, 2-ketoglutarate, 3-oxobutyrate, 2-butanone, 3-oxopyrrolidine (3-OP), 3-pyridylmethylketone (3-PMK), 3-oxobutyric acid ethyl ester (3-OBEE), 3-oxopentanoic acid methyl ester (3-OPME), N -1-boc-3-oxopiperidinone and N -1-boc-3-oxopyrrolidine (B3OP) or a salt, for instance a chloride, of any one thereof.
  • the obtained ketone product is acetone.
  • Suitable reaction conditions refer to those conditions in the transaminase catalyzed reaction solution (e.g., ranges of enzyme loading, substrate loading, cofactor loading, temperature, pH, buffers, co-solvents, etc.) under which the selected transaminase is capable of converting a substrate compound to a product compound (e.g., conversion of the compound of formula (I), preferably (I-a), to the compound of formula (VIII), preferably (VIII-a), in particular (VIII-aa)).
  • exemplary “suitable reaction conditions” are provided in the present disclosure and illustrated by the Examples.
  • Loading such as in “compound loading” or “enzyme loading” or “cofactor loading” refers to the concentration or amount of a component in a reaction mixture at the start of the reaction.
  • Substrate in the context of the transaminase catalyzed reaction process refers to the compound or molecule acted on by the enzyme.
  • an exemplary substrate for the transaminase in the process disclosed herein is compound (I).
  • Product in the context of the transaminase catalyzed reaction process refers to the compound or molecule resulting from the action of the enzyme.
  • an exemplary product for the transaminase in the process disclosed herein is compound (VIII).
  • the transaminase reaction is enantioselective, i.e. produces the desired enantiomer in excess of the undesired enantiomer
  • the term enantiomer refers to the specific stereogenic centre where the amine group is introduced; since the preferred substrate already contains a stereogenic center, in practice the desired compound should have diastereomeric purity).
  • the desired enantiomer is formed in at least 70%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or greater enantiomeric excess (ee).
  • the amino acceptor is converted to the desired chiral amine compound in a conversion rate of more than 50%, or at least 60, 70, 80, 90, 95, 99, in particular 100%.
  • Alkyl is defined as a radical or part of a radical as a straight or branch (one or, if desired and possible, more times) carbon chain, and is especially C 1 -C 7 -alkyl, preferably C 1 -C 6 -alkyl, more preferably C 1 -C 4 -alkyl.
  • C 1 -C 7 - defines a moiety with up to and including maximally 7, especially up to and including maximally 6 and 4 respectively, carbon atoms, said moiety being branched (one or more times) or straight-chained and bound via a terminal or a non-terminal carbon.
  • Cycloalkyl is, for example, C 3 -C 7 -cycloalkyl and is, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl and cycloheptyl. Cyclopentyl and cyclohexyl are preferred.
  • Alkoxy is, for example, C 1 -C 7 -alkoxy and is, for example, methoxy, ethoxy, n-propyloxy, isopropyloxy, n-butyloxy, isobutyloxy, sec-butyloxy, tert -butyloxy and also includes corresponding pentyloxy, hexyloxy and heptyloxy radicals.
  • C 1 -C 4 -alkoxy is preferred.
  • C 2 -C 5 -Alkanoyl is preferred, especially acetyl.
  • Halo or halogen is preferably fluoro, chloro, bromo or iodo, most preferably, chloro, bromo, or iodo.
  • Halo-alkyl is, for example, halo-C 1 -C 7 -alkyl and is in particular halo-C 1 -C 4 -alkyl, such as trifluoromethyl, 1,1,2-trifluoro-2-chloroethyl or chloromethyl.
  • Preferred halo-C 1 -C 7 -alkyl is trifluoromethyl.
  • Alkenyl may be linear or branched alkyl containing a double bond and comprising preferably 2 to 12 carbon atoms, 2 to 10 carbon atoms being especially preferred. Particularly preferred is a linear C 2 -C 7 -alkenyl, more preferably C 2 -C 4 -alkenyl.
  • alkyl groups are ethyl and the isomers of propyl, butyl, pentyl, hexyl, heptyl, octyl, nonyl, decyl, undecyl, dodecyl, tetradecyl, hexadecyl, octacyl and eicosyl, each of which containing a double bond. Especially preferred is allyl.
  • Alkylene is a bivalent radical derived from C 1-7 -alkyl and is especially C 2 -C 7 -alkylene or C 2 -C 7 -alkylene and, optionally, can be interrupted by one or more, e.g. up to three oxygen, NR14 or sulfur, wherein R14 is alkyl, each of which can be unsubstituted or substituted, by one or more substituents independently selected from for example, C 1 -C 7 -alkyl, C 1 -C 7 -alkoxy-C 1 -C 7 -alkyl or C 1 -C 7 -alkoxy.
  • Alkenylene is a bivalent radical derived from C 2-7 -alkenyl and can be interrupted by one or more, e.g. up to three oxygen, NR14 or sulfur, wherein R14 is alkyl, and is unsubstituted or substituted by one or more, e.g. up to three substitutents, preferably independently selected from the substituents mentioned above for alkylene.
  • Aryl being a radical or part of a radical is, for example C 6-10 -aryl, and is preferably a mono- or polycyclic, especially monocyclic, bicyclic or tricyclic aryl moiety with 6 to 10 carbon atoms, such as phenyl, naphthyl or fluorenyl preferably phenyl, and which can be unsubstituted or substituted,by one or more substituents, independently selected from, e.g. C 1 -C 7 -alkyl, C 1 -C 7 -alkoxy-C 1 -C 7 -alkyl or C 1 -C 7 -alkoxy.
  • arylalkyl refers to aryl-C 1 -C 7 -alkyl, wherein aryl is as defined herein and is for example benzyl.
  • carboxyl refers to -CO 2 H.
  • Aryloxy refers to an aryl-O- wherein aryl is as defined above.
  • up to three substitutents preferably independently selected from the group consisting of halo, C 1 -C 7 -alkyl, halo-C 1 -C 7 -alkyl, C 1 -C 7 -alkoxy, halo-C 1 -C 7 -alkoxy, such as trifluoromethoxy and C 1 -C 7 -alkoxy-C 1 -C 7 -alkoxy.
  • the heterocyclyl is an aromatic ring system, it is also referred to as heteroaryl.
  • Heterocyclyl is preferably imizazolyl, pyrazolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, pyridinyl, pyranyl, diazionyl, oxazinyl, thiazinyl, dioxinyl, dithiinyl, azepanyl, oxepanyl, thiepanyl, indolyl, isoindoly, quinolinyl, isoquinolinyl, benzazepinyl, carbazolyl, imidazolidinyl, pyrazolidinyl, oxazolidinyl, isoxazolinidyl, thiazolidy, dioxolanyl, dithiolanyl, furazanyl, oxadiazolyl, thiadiazolyl, dithiazolyl, tetrazolyl, piperidinyl, piperaz
  • heterocyclylalkyl the heterocyclyl is preferably as just defined and is attached to an alkyl as defined for alkyl.
  • alkyl as defined for alkyl. Examples are imidazolylmethyl, pyridylmethyl or piperidinylmethyl.
  • Sulfonyl is (unsubstituted or substituted) C 1 -C 7 -alkylsulfonyl, such as methylsulfonyl, (unsubstituted or substituted) phenyl- or naphthyl-C 1 -C 7 -alkylsulfonyl, such as phenylmethanesulfonyl, or (unsubstituted or substituted) phenyl- or naphthyl-sulfonyl; wherein if more than one substituent is present, e.g.
  • the substituents are selected independently from cyano, halo, halo-C 1 -C 7 -alkyl, halo-C 1 -C 7 -alkyloxy- and C 1 -C 7 -alkyloxy.
  • C 1 -C 7 -alkylsulfonyl such as methylsulfonyl
  • (phenyl- or naphthyl)-C 1 -C 7 -alkylsulfonyl such as phenylmethanesulfonyl.
  • Sulfenyl is (unsubstituted or substituted) C 6-10 -aryl-C 1 -C 7 -alkylsulfenyl or (unsubstituted or substituted) C 6-10 -arylsulfenyl, wherein if more than one substituent is present, e.g. one to four substitutents, the substituents are selected independently from nitro, halo, halo-C 1 -C 7 -alkyl and C 1 -C 7 -alkyloxy.
  • Imide refers to a (unsubstituted or substituted) functional group consisting of two acyl groups bound to nitrogen, preferably a cyclic group derived from dicarboxylic acids. Especially preferred is succinimidyl derived from succinic acid or phthalimidyl derived from phthalic acid.
  • the imidyl group may be substituted by one or more substituents independently selected from for example, C 1 -C 7 -alkyl, C 1 -C 7 -alkoxy-C 1 -C 7 -alkyl, C 1 -C 7 -alkoxy or halo.
  • Silyl refers to a group according to the formula -SiR11R12R13, wherein R11, R12 and R13 are, independently of each other, C 1 -C 7 -alkyl, C 6 -C 10 -aryl or phenyl-C 1 -C 4 -alkyl.
  • R11, R12 and R13 are methyl, ethyl, isopropyl, tert -butyl, phenyl or phenyl-C 1-4 -alkyl.
  • Salts are especially pharmaceutically acceptable salts or generally salts of any of the intermediates mentioned herein, except if salts are excluded for chemical reasons the skilled person will readily understand. They can be formed where salt forming groups, such as basic or acidic groups, are present that can exist in dissociated form at least partially, e.g. in a pH range from 4 to 10 in aqueous solutions, or can be isolated especially in solid, especially crystalline, form.
  • salt forming groups such as basic or acidic groups
  • Such salts are formed, for example, as acid addition salts, preferably with organic or inorganic acids, from compounds or any of the intermediates mentioned herein with a basic nitrogen atom (e.g. imino or amino), especially the pharmaceutically acceptable salts.
  • Suitable inorganic acids are, for example, halogen acids, such as hydrochloric acid, sulfuric acid, or phosphoric acid.
  • Suitable organic acids are, for example, carboxylic, phosphonic, sulfonic or sulfamic acids, for example acetic acid, propionic acid, lactic acid, fumaric acid, succinic acid, citric acid, amino acids, such as glutamic acid or aspartic acid, maleic acid, hydroxymaleic acid, methylmaleic acid, benzoic acid, methane- or ethane-sulfonic acid, ethane-1,2-disulfonic acid, benzenesulfonic acid, 2-naphthalenesulfonic acid, 1,5-naphthalene-disulfonic acid, N -cyclohexylsulfamic acid, N -methyl-, N-ethyl- or N -propyl-sulfamic acid, or other organic protonic acids, such as ascorbic acid.
  • carboxylic, phosphonic, sulfonic or sulfamic acids for example acetic acid, propi
  • salts may also be formed with bases, e.g. metal or ammonium salts, such as alkali metal or alkaline earth metal salts, for example sodium, potassium, magnesium or calcium salts, or ammonium salts with ammonia or suitable organic amines, such as tertiary monoamines, for example triethylamine or tri(2-hydroxyethyl)amine, or heterocyclic bases, for example N-ethylpiperidine or N,N '-dimethylpiperazine.
  • bases e.g. metal or ammonium salts, such as alkali metal or alkaline earth metal salts, for example sodium, potassium, magnesium or calcium salts, or ammonium salts with ammonia or suitable organic amines, such as tertiary monoamines, for example triethylamine or tri(2-hydroxyethyl)amine, or heterocyclic bases, for example N-ethylpiperidine or N,N '-dimethylpiperazine.
  • any of the intermediates mentioned herein may also form internal salts.
  • any reference to "compounds", “starting materials” and “intermediates” hereinbefore and hereinafter is to be understood as referring also to one or more salts thereof or a mixture of a corresponding free compound, intermediate or starting material and one or more salts thereof, each of which is intended to include also any solvate or salt of any one or more of these, as appropriate and expedient and if not explicitly mentioned otherwise.
  • Different crystal forms may be obtainable and then are also included.
  • nitrogen protecting group comprises any group which is capable of reversibly protecting a nitrogen functionality, preferably an amine and/or amide functionality.
  • the nitrogen protecting group is an amine protecting group and/or an amide protecting group.
  • Suitable nitrogen protecting groups are conventionally used e.g. in peptide chemistry and are described e.g. in the relevant chapters of standard reference works such as J. F. W. McOmie, "Protective Groups in Organic Chemistry", Plenum Press, London and New York 1973 , in P. G. M. Wuts and T. W. Greene, “Greene's Protective Groups in Organic Synthesis', fourth edition, Wiley, New Jersey, 2007 , and " The Peptides”; volume 3 (editors: E.
  • Preferred nitrogen protecting groups generally comprise: unsubstituted or substituted C 1 -C 6 -alkyl, preferably C 1 -C 4 -alkyl, more preferably C 1 -C 2 -alkyl, most preferably C 1 -alkyl, unsubstituted or substituted C 2-4 -alkenyl, wherein each C 1 -C 6 -alkyl and C 2-4 -alkenyl is optionally mono-, di- or tri-substituted by trialkylsilyl-C 1 -C 7 -alkoxy (e.g.
  • cycloalkyl cycloalkyl, aryl, preferably phenyl, or a heterocyclic group, preferably pyrrolidinyl, wherein the cycloalkyl group, the aryl ring or the heterocyclic group is unsubstituted or substituted by one or more, e.g. two or three residues, e.g.
  • C 1 -C 7 -alkyl selected from the group consisting of C 1 -C 7 -alkyl, hydroxy, C 1 -C 7 -alkoxy, C 2 -C 8 -alkanoyl-oxy, halogen, nitro, cyano, and CF 3 ; aryl-C 1 -C 2 -alkoxycarbonyl (preferably phenyl-C 1 -C 2 -alkoxycarbonyl e.g. benzyloxycarbonyl); C 1-10 -alkenyloxycarbonyl; C 1-6 -alkylcarbonyl (e.g. acetyl or pivaloyl); C 6-10 -arylcarbonyl; C 1-6 -alkoxycarbonyl (e.g.
  • tert -butoxycarbonyl C 6-10 -aryl-C 1-6 -alkoxycarbonyl; allyl or cinnamyl; sulfonyl or sulfenyl; a succinimidyl group, substituted silyl, e.g. triarylsilyl or trialkylsilyl (e.g. triethylsilyl).
  • nitrogen protecting groups are acetyl, benzyl, cumyl, benzhydryl, trityl, benzyloxycarbonyl (Cbz), 9-fluorenylmethyloxycarbony (Fmoc), benzyloxymethyl (BOM), pivaloyl-oxy-methyl (POM), trichloroethxoycarbonyl (Troc), 1-adamantyloxycarbonyl (Adoc), allyl, allyloxycarbonyl, trimethylsilyl, tert -butyl-dimethylsilyl (TBDMS), triethylsilyl (TES), triisopropylsilyl (TIPS), trimethylsilyethoxymethyl (SEM), tert -butoxycarbonyl (BOC), tert -butyl, 1-methyl-1,1-dimethylbenzyl, (phenyl)methylbenzene, pyrridinyl and pivaloyl.
  • nitrogen protecting groups are acetyl, benzyl, benzyloxycarbonyl (Cbz), triethylsilyl (TES), trimethylsilyethoxymethyl (SEM), tert -butoxycarbonyl (BOC), pyrrolidinylmethyl and pivaloyl.
  • nitrogen protecting groups are, pivaloyl, pyrrolidinylmethyl, t-butoxycarbonyl, benzyl and silyl groups, particularly silyl groups according to the formula SiR11R12R13 group, wherein R11, R12 and R13 are, independently of each other, C 1 -C 7 -alkyl, C 6 -C 10 -aryl or phenyl-C 1 -C 4 -alkyl.
  • Preferred examples for R11, R12 and R13 are methyl, ethyl, isopropyl, t-butyl and phenyl.
  • nitrogen protecting groups are tert -butoxycarbonyl (BOC), benzoyl, styryl, 1-butenyl, benzyl, p-methoxybenzyl (PMB) and pyrrolidinylmethyl, in particular pivaloyl and tert -butoxycarbonyl (BOC).
  • nitrogen protecting group refers to a group which is selected from the group consisting of C 1 -C 6 -alkyl, which is unsubstituted or mono-, di- or tri-substituted by tri-C 1 -C 6 -alkylsilylC 1 -C 7 -alkoxy, C 6 -C 10 -aryl, or a heterocyclic group being a mono-, bi- or tricyclic ring system with 5 to 14 ring atoms and 1 to 4 heteroatoms independently selected from N, O, S, S(O) or S(O) 2 , wherein the aryl ring or the heterocyclic group is unsubstituted or substituted by one, two or three residues, selected from the group consisting of C 1 -C 7 -alkyl, hydroxyl, C 1 -C 7 -alkoxy, C 2 -C 8 -alkanoyl-oxy, halogen, nitro, cyano, and CF 3
  • nitrogen protecting group comprises any group which is capable of reversibly protecting an amino functionality.
  • the removal usually can be carried out by using known methods. e.g. as described in the references cited above.
  • the nitrogen protecting group is removed by using acidic or basic conditions.
  • acidic conditions are hydrochloric acid, trifluoroacetic acid, sulphuric acid.
  • basic conditions are lithium hydroxide, sodium ethoxide. Nucleophiles such as sodium borohydride can be used.
  • N-benzyl as amino protecting group it can be removed by hydrogenation or by the use of some suitable oxidizing agents, e.g. ceric ammonium nitrate (CAN) or 2,3-dichloro-5,6-dicyano-p-benzoquinone (DDQ).
  • CAN ceric ammonium nitrate
  • DDQ 2,3-dichloro-5,6-dicyano-p-benzoquinone
  • a carboxyl protecting group (e.g. Ra or R* in this disclosure) can be any group known in the art, especially C 1 -C 6 -alkyl, e.g. ethyl, methyl, allyl or tert-butyl, or C 6 -C 10 -aryl-C 1 -C 6 -alkyl, e.g. benzyl, or a silyl group SiR11R12R13, wherein R11, R12, and R13 are, independently of each other, C 1 -C 7 -alkyl, C 6 -C 10 -aryl or phenyl-C 1 -C 4 -alkyl.
  • R11, R12 and R13 are methyl, ethyl, isopropyl, tert -butyl, phenyl or phenyl-C 1-4 -alkyl.
  • the carboxyl protecting groups themselves, their introduction reactions, and their removal reactions are described for example in standard reference works, such as J. F. W. McOmie, "Protective Groups in Organic Chemistry", Plenum Press, London and New York 1973 , in T. W. Greene, “Protective Groups in Organic Synthesis", Third edition, Wiley, New York 1999 , in “ The Peptides”; Volume 3 (editors: E. Gross and J.
  • an C 1 -C 6 -alkyl, e.g. ethyl, protecting group R* or Ra can be removed by hydrolysis, e.g. in the presence of a base, such as an alkaline metal hydroxide, e.g. lithium hydroxide, in the presence of an appropriate solvent, e.g. a cyclic ether, such as tetrahydrofuran, and water, e.g. at a temperature in the range from 0 to 50 °C, such as from 10 to 30 °C.
  • a base such as an alkaline metal hydroxide, e.g. lithium hydroxide
  • an appropriate solvent e.g. a cyclic ether, such as tetrahydrofuran
  • water e.g. at a temperature in the range from 0 to 50 °C, such as from 10 to 30 °C.
  • protecting group is only used as such if it is removed for the next to follow product - if it remains, the protecting group is becoming a substituent.
  • alkyl such as ethyl, if removed, is a protecting group, if it remains, it becomes a permanent moiety.
  • alkyl such as ethyl
  • alkyl such as ethyl
  • the deprotection and decarboxylation reactions preferably take place in the presence of an acid such as an inorganic acid, e.g. a hydrohalic acid, such as hydrochloric acid, in an appropriate solvent or solvent mixture, e.g. in a mixture of an organic acid, such as acetic acid, and water, e.g. at elevated temperatures from 50°C to the reflux temperature of the reaction mixture, such as from 90 to 120°C; or with an alkali hydroxide, such as sodium hydroxide, in an appropriate solvent or solvent mixture, such as an alcohol, e.g. ethanol, or an ether, e.g.
  • an acid such as an inorganic acid, e.g. a hydrohalic acid, such as hydrochloric acid
  • an appropriate solvent or solvent mixture e.g. in a mixture of an organic acid, such as acetic acid, and water, e.g. at elevated temperatures from 50°C to the reflux temperature of the reaction mixture, such as from 90 to 120°C; or
  • tetrahydrofurane or 1,4-dioxane at temperatures e.g. in the range of from 30°C to the reflux temperature of the reaction mixture, e.g. from 40 to 90°C, followed by acidification, e.g. with HCI, for decarboxylation; or with an alkali chloride, such as sodium chloride, in the presence of an appropriate solvent, e.g. DMSO e.g.
  • R* is tert-butyl and Ra is a (not removed) ethyl, in the presence of an acid, such as trifluoroacetic acid in an appropriate solvent or solvent mixture and at customary temperatures; or if R* is benzyl or allyl, by hydrogenation in the presence of a hydrogenation catalyst, such as a noble metal, e.g. Pd or Pd on charcoal, in an appropriate solvent or solvent mixture, e.g. an alcohol, such as methanol or ethanol.
  • a hydrogenation catalyst such as a noble metal, e.g. Pd or Pd on charcoal
  • the introduction of a moiety Ra other than hydrogen into a deprotected and decarboxylated compound can preferably be conducted from the corresponding resulting compound of the formula (I), especially (I-a) wherein Ra is hydrogen by reaction with an alcohol of the formula Ra-OH, wherein Ra is as defined for a compound of the formula (I), especially Ra is C 1 -C 6 -alkyl, such as ethyl, in the presence of an activating agent, e.g. an agent to transform the acid into an acyl halide or acid anhydride.
  • an activating agent e.g. an agent to transform the acid into an acyl halide or acid anhydride.
  • the reaction preferably takes place in a customary solvent, such as an C 1 -C 6 -alkyl-alcohol, e.g. methanol or ethanol, and at appropriate temperatures, e.g. in the range from 0 to 100°C, e.g. from 10 to 90°C.
  • a customary solvent such as an C 1 -C 6 -alkyl-alcohol, e.g. methanol or ethanol
  • the leaving group X is preferably halo, e.g. bromo, iodo or chloro, or an activated alcohol group, e.g. mesylate or tosylate.
  • This reaction preferably takes place in the presence of a tetraalkylammonium halogenide, e.g. tetrabutylammonium iodide, and a base, e.g. an alkali metal carbonate, such as potassium carbonate, in an appropriate solvent, e.g. acetone, preferably at temperatures in the range from -10 to 50 °C, e.g. at -5 to 30 °C.
  • a tetrabutylammonium hydrogen sulfate in toluene at reflux e.f. e.g.
  • the compound of the formula (III) can, in a further embodiment of the invention, preferably be obtained by a process comprising reaction of an acetic acid compound of the formula (V), or a reactive derivative thereof, with a salt of a malonic acid half ester of the formula (VI), wherein R* is a carboxyl protecting group.
  • reaction conditions are well-known in the art.
  • the reaction preferably takes place in the presence of a coupling reagent, such as N,N -carbonylimidazole, in an appropriate solvent or solvent mixture, e.g. an ether, such as tetrahydrofuran, and/or a nitrile, such as acetonitrile, in the presence of an alkaline earth metal salt, such as magnesium chloride, and a base, e.g. a tertiary nitrogen base, such as trimethylamine, at preferred temperatures in the range from -20 to 50°C, e.g. from -10 to 30°C.
  • a coupling reagent such as N,N -carbonylimidazole
  • an appropriate solvent or solvent mixture e.g. an ether, such as tetrahydrofuran, and/or a nitrile, such as acetonitrile
  • an alkaline earth metal salt such as magnesium chloride
  • a base e.
  • a coupling reagent e.g. dicyclohexyl diimidazole in combination with Meldrum's acid in dichloromethane at 0-20 °C and subsequent opening of the intermediate product with an alcohol, e.g. ethanol, potentially in the presence of an acid catalyst, e.g. p-toluene sulfonic acid (c.f. e.g. US 2004/2508 A1 ), or (c) use of an activated mixed anhydride, e.g. prepared by treatment of the acid with an alkyl chloroformate, e.g.
  • a coupling reagent e.g. dicyclohexyl diimidazole in combination with Meldrum's acid in dichloromethane at 0-20 °C and subsequent opening of the intermediate product with an alcohol, e.g. ethanol, potentially in the presence of an acid catalyst, e.g. p-toluene sulfonic acid (c.f. e.g
  • ethyl chloroformate potentially in combination with an alkyl magnesium halide or an alkyl lithium reagent as base for deprotonation of the monoalkyl malonate alkali salt (c.f. e.g. J. Chem. Soc., Perkin Trans. 1, 2002, 1663-1671 ; (b) WO2008/36420 A2 ) in THF at reduced temperature, e.g. -30 °C.
  • the compound of the formula (III) can, in a further embodiment of the invention, preferably be obtained by a process comprising reacting an intermediate compound of formula (XIV), preferably of formula (XIV-a), or a salt thereof, wherein Rf is selected from a group -O-R* wherein R* is selected from a carboxyl protecting group and C 1 -C 6 -alkyl, preferably methyl; - N(CH3)-O(CH3) (Weinreb-amide), morpholinyl, and imidazolinyl, with an in situ prepared activated dianionic derivative of the compound of formula (V), or a salt thereof, namely a compound of formula (V*), in the presence of a base, and followed by a decarboxylation reaction, to obtain the compound of formula (I), or a salt thereof wherein Ra is hydrogen.
  • Rf is selected from a group -O-R* wherein R* is selected from a carboxyl protecting group and C 1 -C 6 -
  • the starting compound of formula (XIV) or (XIV-a) is known in the art and commercially available, or can be easily obtained from a compound of the following formula by methods known in the art; in particular the starting compound of formula (XIV-a) can be obtained e.g. by asymmetric hydrogenation in the presence of a chiral catalyst.
  • a particular preferred group Rf e.g. a methoxy group, a Weinreb amide or secondary amide group are also well known in the art.
  • the activated dianionic derivative of the compound of formula (V), a so-called Ivanov-reagent (V*), can be prepared in situ by bis-deprotonation of the compound of formula (V) by treatment with very strong bases, e.g. alkyl lithium reagents, lithium amide bases or Grignard reagent ( Synthesis 1982, 521-578 and references cited therein).
  • very strong bases e.g. alkyl lithium reagents, lithium amide bases or Grignard reagent ( Synthesis 1982, 521-578 and references cited therein).
  • the deprotonation is preferably performed by addition of a Grignard reagent, such as isopropyl magnesium chloride or sec-butyl magnesium bromide, preferably in the presence of LiCI (e.g.
  • l-PrMgCl ⁇ LiCl so-called TurboGrignard, available from Sigma-Aldrich, Cat No 656984
  • an appropriate solvent e.g. an ether, such as tetrahydrofuran, at preferred temperatures in the range from -20 to 50 °C, e.g. from -10 to 40 °C.
  • the activated dianionic derivative of formula (V*) is then added to the compound of formula (XIV) in the presence of a base, or alternative to a salt of the compound of formula (XIV).
  • Suitable bases are e.g. a Grignard reagent, such as isopropyl magnesium chloride or sec-butyl magnesium bromide, or strong bases like sodium hydride.
  • the addition is preferably performed in an appropriate solvent, e.g. an ether, such as tetrahydrofuran, at preferred temperatures in the range from -20 to 70 °C, e.g. from -20 to 30 °C, for a period of 1h to 24h.
  • the subsequent decarboxylation reaction then preferably takes place in the presence of an acid such as an inorganic acid, e.g. a hydrohalic acid, such as hydrochloric acid, in an appropriate solvent or solvent mixture, e.g. in a mixture of an organic acid, such as acetic acid, and water, or in an organic solvent, e.g. tetrahydrofuran, and is carried out at temperatures from 0°C to room temperature.
  • an acid such as an inorganic acid, e.g. a hydrohalic acid, such as hydrochloric acid
  • an appropriate solvent or solvent mixture e.g. in a mixture of an organic acid, such as acetic acid, and water, or in an organic solvent, e.g. tetrahydrofuran
  • decarboxylation can also be achieved by heating to the reflux temperature of the solvent.
  • the obtained compound of formula (I), or a salt thereof, wherein Ra is hydrogen can be reacted with an agent introducing a carboxyl protecting group, to provide the compound of formula (I), wherein Ra is a carboxyl protecting group, or the obtained compound of formula (I), or a salt thereof, wherein Ra is hydrogen, is reacted with a coupling reagent in the presence of an C 1 -C 6 -alkanol, especially ethanol, to provide the compound of formula (I), wherein Ra is C 1 -C 6 -alkyl, preferably ethyl, according to procedures as e.g. described for SCHEME 1 above or in the general part for carboxyl protectin groups.
  • the compound of the formula (V) can, for example, in a further embodiment of the invention preferably be obtained by a process comprising hydrolysing a cyanide of the formula (VII).
  • the hydrolysis can preferably take place in the presence of a base, e.g. an alkali metal hydroxide, such as potassium hydroxide, in an appropriate solvent, e.g. an alcohol, such as a diol, e.g. ethylene glycol, at elevated temperatures, e.g. from 30°C to the reflux temperature of the reaction mixture.
  • a base e.g. an alkali metal hydroxide, such as potassium hydroxide
  • an appropriate solvent e.g. an alcohol, such as a diol, e.g. ethylene glycol
  • the compound of formula V (biphenyl acetic acid) is also available commercially (Sigma-Aldrich, catalogue no. 196487, CAS no. 5728-52-9 ).
  • NEP inhibitors or prodrugs thereof in particular NEP-inhibitors, or prodrugs thereof, such as sacubitril.
  • the compound of the formula (I), especially (I-a), can thus be used (in a further invention embodiment, alone or with the immediately or other preceding and following step or steps) in the manufacture of a compound of the formula (VIII), especially of the formula (VIII-a), or a salt thereof, respectively.
  • This transformation can be carried out in a number of different ways as detailed in SCHEMEs 6 to 10.
  • the transformation comprises a process (forming an invention embodiment, alone or with the immediately or other preceding and following step or steps) which comprises reacting a compound of the formula (I), especially (I-a), wherein Ra is hydrogen, or a salt thereof, with ammonia, a primary or secondary amine, or salts thereof, yielding a compound of the formula (VIII), wherein Ra is hydrogen, if starting from a compound of the formula (I), especially (I-a), wherein Ra is hydrogen, or a salt thereof.
  • the compound of the formula (VIII) can also be obtained from the compound of formula (I), especially formula (I-a), with a primary or secondary amine such as benzyl amine or a chiral benzyl amine, e.g. phenethylamine, to obtain the corresponding (benzyl-) protected amine, which is then transformed into the amino acid (VIII) by hydrogenolytic cleavage of the respective (benzyl-) protected amine.
  • a primary or secondary amine such as benzyl amine or a chiral benzyl amine, e.g. phenethylamine
  • ammonium salt e.g. ammonium formate, ammonium acetate or ammonium hydroxide
  • ammonia itself alone or in combination with titanium alkoxides, e.g. titanium isopropoxide
  • hydroxylamine salts such as the hydroxylamine hydrochloride
  • an appropriate reducing agent such as sodium borohydride, sodium tris(acetoxy)borohydride or sodium cyanoborohydride, or reduction can also occur by hydrogenation, usually with Pd-catalysts (e.g.
  • 10% Pd/C) or Raney-Ni in an appropriate solvent or solvent mixture, e.g. in water, an alcohol, such as ethanol, and/or an ether, such as tetrahydrofuran, or a mixture of two or more thereof, at preferred temperatures in the range from 10 to 50°C or up to 100°C, e.g. from 20 to 40 or up to 80°C.
  • an appropriate solvent or solvent mixture e.g. in water, an alcohol, such as ethanol, and/or an ether, such as tetrahydrofuran, or a mixture of two or more thereof, at preferred temperatures in the range from 10 to 50°C or up to 100°C, e.g. from 20 to 40 or up to 80°C.
  • This compound of the formula (VIII), especially (VIII-a), or a salt thereof can then be used, in a further invention embodiment, for the manufacture of a corresponding compound of the formula (VIII), especially (VIII-a), wherein Ra is C 1 -C 6 -alkyl, e.g. by reacting a compound of the formula (VIII), especially (VIII-a), wherein Ra is hydrogen, with an agent introducing an amino protecting group, e.g. tert -butoxycarbonyl, followed by reaction of the amino protected analogue of the compound of the formula (VIII), especially (VIII-a), with an activating reagent, e.g.
  • an activating reagent e.g.
  • the transformation comprises a process (forming an invention embodiment, alone or with the immediately or other preceding and following step or steps), which comprises converting a compound of the formula (I), especially (I-a), or a salt thereof, wherein Ra is selected from hydrogen and C 1 -C 6 -alkyl, preferably hydrogen, into a compound of formula (VIII), in particular (VIII-a), preferably to the compound of formula (VIII-a), in particular (VIII-aa), wherein Ra is selected from hydrogen and C 1 -C 6 -alkyl, preferably hydrogen, and Re is hydrogen, or a salt thereof, by bringing it in contact with an (S)-selective ⁇ -transaminase in the presence of an amine donor and a coenzyme.
  • a process forming an invention embodiment, alone or with the immediately or other preceding and following step or steps), which comprises converting a compound of the formula (I), especially (I-a), or a salt thereof, wherein Ra is selected from hydrogen and C 1
  • the conversion rate from the compound of formula (I) to the compound of formula (VIII), preferably to the compound of formula (VIII-1), especially the conversion rate from the compound of formula (I-a) to the compound of formula (VIII-a), more preferably of formula (VIII-aa), is more than 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 95%, at least about 98%, or at least about 99%., or even 100%.
  • the transaminase is capable of converting the substrate compound of formula (I) and (I-a), respectively, in particular compound (R)-5-([1,1'-biphenyl]-4-yl)-2-methyl-4-oxopentanoic acid, to the product compound of formula (VIII-1) and (VIII-aa), respectively, in particular compound (2R,4S)-5-([1,1'-biphenyl]-4-yl)-4-amino-2-methylpentanoic acid, in enantiomeric excess (with regard to the stereogenic centre of the transamination) of greater than 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5% or greater over the corresponding compound of formula (VIII-1b) and (VIII-ab), respectively, wherein Ra is selected from hydrogen and C 1 -C 6 -alkyl, preferably hydrogen, and Re is hydrogen, in particular over
  • the transaminases used in the instant disclosure are capable of converting compound (I) to compound (VIII-1) and compound (I-a) to compound (VIII-aa), respectively, with increased tolerance for the presence of substrate under suitable reaction conditions.
  • the transaminases are capable of converting the substrate compound (I) and (I-a) to product compound (VIII-1) and (VIII-aa), respectively, in the presence of a substrate loading concentration of at least about 1 g/L, about 5 g/L, about 10 g/L, about 20 g/L, about 30 g/L, about 40 g/L, about 50 g/L, about 70 g/L, about 100 g/L, about 125 g/L, about 150 g/L.
  • Such substrate loading still achieves a percent conversion of at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 95%, at least about 98%, or at least about 99%, or 100%, in a reaction time of about 120 h or less, about 96 h or less, about 72 h or less, about 48 h or less, about 36 h or less, or about 24 h or less, under suitable reaction conditions.
  • the suitable reaction conditions to achieve such conversion rates can be determined with respect to concentrations or amounts of transaminase, substrate, cofactor, buffer, cosolvent, pH, and/or conditions including temperature and reaction time, as further described below and in the Examples.
  • ⁇ -transaminases obtainable from Codexis Inc. under the reference number ATA-217 (part of the Codex® ATA Screening Kit), as well as further genetically modified ⁇ -transaminases variants thereof (also obtainable from Codexis Inc.) were used.
  • Such genetically modified ⁇ -transaminases are described e.g. in US patent No 8,470,564 .
  • reaction conditions including but not limited, to ranges of amino donor, pH, temperature, buffer, solvent system, substrate loading, enzyme (transaminase) loading, cofactor loading, pressure, and reaction time.
  • suitable reaction conditions for carrying out the claimed transaminase process can be readily optimized in view of the guidance provided herein by routine experimentation that includes, but is not limited to, contacting the transaminase and substrate compound under experimental reaction conditions of concentration, pH, temperature, solvent conditions, and detecting the product compound.
  • the transaminase uses isopropylamine (also referred to herein as "IPM") as amine donor.
  • Suitable reaction conditions comprise the amine donor, in particular IPM, present at a concentration of at least about 0.1 to about 3.0 M, 0.2 to about 2.5 M, about 0.5 to about 2 M or about 1 to about 2 M.
  • the amino donor is present at a concentration of about 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 1.0, 1.5, 2.0, 2.5 or 3.0 M.
  • Higher concentrations of amine donor, e.g., IPM can be used to shift the equilibrium towards amine product formation.
  • Suitable reaction conditions also typically comprise a cofactor.
  • the cofactors is pyridoxal-5'-phosphate (also known as pyridoxal-phosphate, PLP, P5P).
  • the cofactor PLP is present naturally in the cell extract and does not need to be supplemented.
  • the suitable reaction conditions comprise cofactor added to the enzyme reaction mixture, for example, when using partially purified, or purified transaminase enzyme.
  • Suitable reaction conditions can comprise the presence of a cofactor preferably PLP, at a concentration of about 0.1 g/L to about 10 g/L, about 0.2 g/L to about 5 g/L, about 0.5 g/L to about 2.5 g/L.
  • the reaction conditions comprise a PLP concentration of about 0.1 g/L or less, 0.2 g/L or less, 0.5 g/L or less, 1 g/L or less, 2.5 g/L or less, 5 g/L or less, or 10 g/L or less.
  • the cofactor can be added either at the beginning of the reaction and/or additional cofactor is added during the reaction.
  • the concentration of the substrate compound of formula (I) or (I-a) in the reaction mixture can be varied, taking into consideration, for example, the desired amount of product compound, the effect of substrate concentration on enzyme activity, stability of enzyme under reaction conditions, and the percent conversion of substrate to product.
  • the suitable reaction conditions comprise a substrate compound loading of at least about 0.5 to about 200 g/L, 1 to about 200 g/L, 5 to about 150 g/L, about 10 to about 100 g/L, 20 to about 100 g/L or about 50 to about 100 g/L.
  • the suitable reaction conditions comprise a substrate compound loading of at least about 0.5 g/L, at least about 1 g/L, at least about 5 g/L, at least about 10 g/L, at least about 15 g/L, at least about 20 g/L, at least about 30 g/L, at least about 50 g/L, at least about 75 g/L, at least about 100 g/L, at least about 150 g/L or at least about 200 g/L, or even greater.
  • Suitable reaction conditions comprise a transaminase concentration of about 0.01 to about 50 g/L; about 0.05 to about 50 g/L; about 0.1 to about 40 g/L; about 1 to about 40 g/L; about 2 to about 40 g/L; about 5 to about 40 g/L; about 5 to about 30 g/L; about 0.1 to about 10 g/L; about 0.5 to about 10 g/L; about 1 to about 10 g/L; about 0.1 to about 5 g/L; about 0.5 to about 5 g/L; or about 0.1 to about 2 g/L.
  • the transaminase concentration at about 0.01, 0.05, 0.1, 0.2, 0.5, 1, 2, 5, 10, 15, 20, 25, 30, 35, 40, or 50 g/L.
  • the pH of the reaction mixture may change.
  • the pH of the reaction mixture may be maintained at a desired pH or within a desired pH range. This may be done by the addition of an acid or a base, before and/or during the course of the reaction.
  • the pH may be controlled by using a buffer.
  • the suitable reaction conditions comprise a solution pH comprise a pH from about 6 to about 12, pH from about 6 to about 10, pH from about 6 to about 8, pH from about 7 to about 10, pH from about 7 to about 9, or pH from about 7 to about 8.
  • the reaction conditions comprise a solution pH of about 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10. 10.5, 11, 11.5 or 12.
  • the reaction condition comprises a buffer.
  • Suitable buffers to maintain desired pH ranges are known in the art and include, by way of example and not limitation, borate, carbonate, phosphate, triethanolamine buffer, and the like.
  • the buffer is borate.
  • the suitable reaction conditions comprise a buffer solution of triethanolamine, where the triethanolamine concentration is from about 0.01 to about 0.4 M, 0.05 to about 0.4 M, 0.1 to about 0.3 M, or about 0.1 to about 0.2 M.
  • the reaction condition comprises a triethanolamine concentration of about 0.01, 0.02, 0.03, 0.04, 0.05, 0.07, 0.1, 0.12, 0.14, 0.16, 0.18, 0.2, 0.3, or 0.4 M.
  • the reaction conditions comprise water as a suitable solvent with no buffer present.
  • a suitable temperature can be used for the reaction conditions, for example, taking into consideration the increase in reaction rate at higher temperatures, and the activity of the enzyme during the reaction time period. Accordingly, in some embodiments, the suitable reaction conditions comprise a temperature of about 10°C to about 70°C, about 10°C to about 65°C, about 15°C to about 60°C, about 20°C to about 60°C, about 20°C to about 55°C, about 30°C to about 55°C, or about 40°C to about 50°C.
  • the suitable reaction conditions comprise a temperature of about 10°C, 15°C, 20°C, 25°C, 30°C, 35°C, 40°C, 45°C, 50°C, 55°C, 60°C, 65°C, or 70°C.
  • the temperature during the enzymatic reaction can be maintained at a temperature throughout the course of the reaction. In some embodiments, the temperature during the enzymatic reaction can be adjusted over a temperature profile during the course of the reaction.
  • Suitable solvents include water, aqueous buffer solutions, organic solvents, polymeric solvents, and/or co-solvent systems, which generally comprise aqueous solvents, organic solvents and/or polymeric solvents.
  • the aqueous solvent water or aqueous co-solvent system
  • the transaminase processes are generally carried out in an aqueous co-solvent system comprising an organic solvent (e.g ., ethanol, isopropanol (IPA), dimethyl sulfoxide (DMSO), ethyl acetate, butyl acetate, 1-octanol, heptane, octane, methyl t butyl ether (MTBE), toluene, and the like), ionic or polar solvents (e.g., 1-ethyl-4-methylimidazolium tetrafluoroborate, 1-butyl-3-methylimidazolium tetrafluoroborate, 1-butyl-3-methylimidazolium hexafluorophosphate, glycerol, polyethylene glycol, and the like).
  • an organic solvent e.g ethanol, isopropanol (IPA), dimethyl sulfoxide (DMSO), ethyl acetate, but
  • the co-solvent can be a polar solvent, such as a polyol, dimethylsulfoxide, DMSO, or lower alcohol.
  • the non-aqueous co- solvent component of an aqueous co-solvent system may be miscible with the aqueous component, providing a single liquid phase, or may be partly miscible or immiscible with the aqueous component, providing two liquid phases.
  • Exemplary aqueous co-solvent systems can comprise water and one or more co-solvents selected from an organic solvent, polar solvent, and polyol solvent.
  • the co-solvent component of an aqueous co-solvent system is chosen such that it does not adversely inactivate the transaminase enzyme under the reaction conditions.
  • Appropriate co-solvent systems can be readily identified by measuring the enzymatic activity of the specified transaminase enzyme with a defined substrate of interest in the candidate solvent system, utilizing an enzyme activity assay, such as those described herein.
  • the suitable reaction conditions comprise an aqueous co-solvent, where the co-solvent comprises a polyol solvent, particularly glycols.
  • suitable polyol solvents include, by way of example and not limitation, polyethylene glycol, polyethylene glycol methyl ether, diethylene glycol dimethyl ether, triethylene glycol dimethyl ether, and polypropylene glycol.
  • the aqueous co-solvent comprises polyethylene glycol, which is available in different molecular weights. Particularly useful are lower molecular weight glycols, such as PEG200 to PEG600.
  • the aqueous co-solvent comprises PEG200 of about 1% to about 40% v/v; about 1% to about 40% v/v; about 2% to about 40% v/v; about 5% to about 40% v/v; 2% to about 30% v/v; 5% to about 30% v/v; 1 to about 20% v/v; about 2% to about 20% v/v; about 5% to about 20% v/v; about 1% to about 10% v/v; about 2% to about 10% v/v.
  • the suitable reaction conditions comprises an aqueous co-solvent comprising PEG200 at about 1%, 2%, 5%, 10%, 15%, 20%; 25%; 30%; 35%; 35% or about 40% v/v.
  • the suitable reaction conditions comprise an aqueous co-solvent, where the co-solvent comprises DMSO at about 1% to about 80% (v/v), about 1 to about 70% (v/v), about 2% to about 60% (v/v), about 5% to about 40% (v/v), 10% to about 40% (v/v), 10% to about 30% (v/v), or about 10% to about 20% (v/v).
  • the suitable reaction conditions comprise an aqueous co-solvent comprising DMSO at least about 1%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, or 80% (v/v).
  • the quantities of reactants used in the transamination reaction will generally vary depending on the quantities of product desired, and concomitantly the amount of transaminase substrate employed. Those having ordinary skill in the art will readily understand how to vary these quantities to tailor them to the desired level of productivity and scale of production.
  • the order of addition of reactants is not critical.
  • the reactants may be added together at the same time to a solvent (e.g., monophasic solvent, biphasic aqueous co-solvent system, and the like), or alternatively, some of the reactants may be added separately, and some together at different time points.
  • a solvent e.g., monophasic solvent, biphasic aqueous co-solvent system, and the like
  • some of the reactants may be added separately, and some together at different time points.
  • the cofactor, transaminase, and transaminase substrate may be added first to the solvent.
  • the solid reactants may be provided to the reaction in a variety of different forms, including powder (e.g., lyophilized, spray dried, and the like), solution, emulsion, suspension, and the like.
  • the reactants can be readily lyophilized or spray dried using methods and equipment that are known to those having ordinary skill in the art.
  • the protein solution can be frozen at -80°C in small aliquots, then added to a prechilled lyophilization chamber, followed by the application of a vacuum.
  • the transaminase, and cofactor may be added and mixed into the aqueous phase first.
  • the organic phase may then be added and mixed in, followed by addition of the transaminase substrate.
  • the transaminase substrate may be premixed in the organic phase, prior to addition to the aqueous phase.
  • the transamination reaction is generally allowed to proceed until further conversion of ketone substrate to amine product does not change significantly with reaction time, e.g., less than 10% of substrate being converted, or less than 5% of substrate being converted. In some embodiments, the reaction is allowed to proceed until there is complete or near complete conversion of substrate ketone to product amine. Transformation of substrate to product can be monitored using known methods by detecting substrate and/or product. Suitable methods include gas chromatography, HPLC, and the like. Conversion yields of the chiral amine product generated in the reaction mixture are generally greater than about 50%, may also be greater than about 60%, may also be greater than about 70%, may also be greater than about 80%, may also be greater than 90%, and are often greater than about 97%.
  • the transamination reaction will proceed for a reaction time of about 120 h or less, about 96 h or less, about 72 h or less, about 48 h or less, about 36 h or less, about 24 h or less, about 18 h or less, or about 12 h or less, under suitable reaction conditions.
  • the methods for preparing compounds of formula (VIII-1) and (VIII-aa) using a transaminase under suitable reaction conditions results in at least about 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or greater conversion of ketone substrate, e.g, compound of formula (I) and (I-a), respectively, to the amine product compound, e.g., compound of formula (VIII-1) and (VIII-aa), respectively, in about 120 h or less, in about 96 h or less, in about 72 h or less, in about 48 h or less, in about 36 h or less, in about 24 h or less, or even less time.
  • the suitable reaction conditions comprise an initial substrate loading to the reaction solution which is then contacted with the transaminase.
  • This reaction solution is the further supplemented with additional substrate of compound as a continuous addition over time at a rate of at least about 1 g/L/h, at least about 2 g/L/h, at least about 4 g/L/h, at least about 6 g/L/h, or higher.
  • transaminase is added to a solution having an initial substrate loading of at least about 20 g/L, 30 g/L, or 40 g/L.
  • transaminase is then followed by continuous addition of further substrate to the solution at a rate of about 2 g/L/h, 4 g/L/h, or 6 g/L/h until a much higher final substrate loading of at least about 30 g/L, 40 g/L, 50 g/L, 60 g/L, 70 g/L, 100 g/L, 150 g/L, 200 g/L or more, is reached.
  • the suitable reaction conditions comprise addition of the transaminase to a solution having an initial substrate loading of at least about 20 g/L, 30 g/L, or 40 g/L followed by addition of further substrate to the solution at a rate of about 2 g/L/h, 4 g/L/h, or 6 g/L/h until a final substrate loading of at least about 30 g/L, 40 g/L, 50 g/L, 60 g/L, 70 g/L, 100 g/L or more, is reached.
  • the further substrate added is in a solution comprising isopropylamine or isopropylamine acetate at a concentration of at least about 0.5 M, at least about 1.0 M, at least about 2.5 M, at least about 5.0 M, at least about 7.5 M, at least about 10.0 M.
  • the transamination reaction can comprise the following suitable reaction conditions: (a) substrate loading at about 5 g/L to 200 g/L; (b) about 0.1 to 50 g/L of transaminase; (c) about 0.1 to 4 M of isopropylamine (IPM); (d) about 0.1 to 10 g/L of pyridoxal phosphate (PLP) cofactor; (e) pH of about 6 to 10; and (f) temperature of about 30 to 60°C.
  • suitable reaction conditions (a) substrate loading at about 5 g/L to 200 g/L; (b) about 0.1 to 50 g/L of transaminase; (c) about 0.1 to 4 M of isopropylamine (IPM); (d) about 0.1 to 10 g/L of pyridoxal phosphate (PLP) cofactor; (e) pH of about 6 to 10; and (f) temperature of about 30 to 60°C.
  • IPM isopropylamine
  • PBP pyr
  • the transamination reaction can comprise the following suitable reaction conditions: (a) substrate loading at about 10 to about 80 g/L; (b) about 0.5 to 25 g/L of transaminase; (c) about 0 to 10% v/v of PEG200; (d) about 0.1 to 2 M of isopropylamine (IPM); (e) about 0.1 to 1 g/L of pyridoxal phosphate (PLP) cofactor; (f) pH of about 8 to 10; (g) temperature of about 40 to 55°C, and (h) reaction times of 18 hr to 36 hr.
  • suitable reaction conditions (a) substrate loading at about 10 to about 80 g/L; (b) about 0.5 to 25 g/L of transaminase; (c) about 0 to 10% v/v of PEG200; (d) about 0.1 to 2 M of isopropylamine (IPM); (e) about 0.1 to 1 g/L of pyridoxal phosphate (PL
  • the transamination reaction can comprise the following suitable reaction conditions: (a) substrate loading of about 25 to about 100 g/L; (b) about 0.5 to 10 g/L of transaminase; (c) about 0 to 10% v/v of PEG200; (d) about 0.1 to 2 M of isopropylamine (IPM); (e) about 0.1 to 1 g/L of pyridoxal phosphate (PLP) cofactor; (f) about 0.005 to about 0.1 M of borate (or comparable) buffer; (g) pH of about 8 to 10; and (h) temperature of about 40 to 55°C.
  • suitable reaction conditions (a) substrate loading of about 25 to about 100 g/L; (b) about 0.5 to 10 g/L of transaminase; (c) about 0 to 10% v/v of PEG200; (d) about 0.1 to 2 M of isopropylamine (IPM); (e) about 0.1 to 1 g/L of pyridoxal
  • reaction components or additional techniques carried out to supplement the reaction conditions can include taking measures to stabilize or prevent inactivation of the enzyme, reduce product inhibition, shift reaction equilibrium to product formation.
  • additional quantities of the amino acceptor can be added (up to saturation) and/or the amino acceptor (ketone) formed can be continuously removed from the reaction mixture.
  • a solvent bridge or a two phase co-solvent system can be used to move the amine product to an extraction solution, and thereby reduce inhibition by amine product and also shift the equilibrium towards product formation (see, e.g., Yun and Kim, 2008, Biosci. Biotechnol. Biochem. 72(11):3030-3033 ).
  • the carbonyl by-product can be removed by sparging the reaction solution with a non-reactive gas or by applying a vacuum to lower the reaction pressure and removing the carbonyl by-product present in the gas phase.
  • a non-reactive gas is any gas that does not react with the reaction components.
  • Various non-reactive gases include nitrogen and noble gases (e.g ., inert gases). In some embodiments, the non-reactive gas is nitrogen gas.
  • the amino donor used in the process is isopropylamine (IPM), which forms the carbonyl by-product acetone upon transfer of the amino group to the amino group acceptor.
  • IPM isopropylamine
  • the acetone can be removed by sparging with nitrogen gas or applying a vacuum to the reaction solution and removing the acetone from the gas phase by an acetone trap, such as a condenser or other cold trap.
  • the acetone can be removed by reduction to isopropanol using a transaminase.
  • the corresponding amino group donor can be added during the transamination reaction to replenish the amino group donor and/or maintain the pH of the reaction. Replenishing the amino group donor also shifts the equilibrium towards product formation, thereby increasing the conversion of substrate to product.
  • isopropylamine can be added to the solution to replenish the amino group donor lost during the acetone removal.
  • any of the above described processes for the conversion of substrate compound to product compound can further comprise one or more steps selected from: extraction; isolation; purification; and crystallization of product compound.
  • Methods, techniques, and protocols for extracting, isolating, purifying, and/or crystallizing the product amine from biocatalytic reaction mixtures produced by the above disclosed methods are known to the ordinary artisan and/or accessed through routine experimentation. Additionally, illustrative methods are provided in the Examples below.
  • the obtained compound of formula (VIII) or (VIII-a), especially (VIII-1) or (VIII-aa), or a salt thereof, wherein Ra and Re are hydrogen can then be used, in a further invention embodiment, for the manufacture of a corresponding compound of the formula (VIII) or (VIII-a), especially (VIII-1) and (VIII-aa), wherein Ra is hydrogen and Re is a nitrogen protecting group such as tert -butoxycarbonyl, e.g. by reacting the compound of the formula (VIII), especially (VIII-1) or (VIII-aa), wherein Ra is hydrogen, with an agent introducing such an amino protecting group, e.g. tert- butoxycarbonyl.
  • the optionally amino protected analogue of the compound of the formula (VIII) or (VIII-a), especially (VIII-1) or (VIII-aa), wherein Ra is hydrogen and Re is hydrogen or a nitrogen protecting group, preferably hydrogen, can then be reacted with an activating reagent, e.g. thionyl chloride, in the presence of an alcohol, especially ethanol, to yield the corresponding compound of the formula (VIII) or (VIII-a), especially (VIII-1) or (VIII-aa), wherein Ra is C 1 -C 6 -alkyl, especially ethyl, e.g. at temperatures in the range from 50 to 80°C, e.g. 65 to 75°C.
  • an activating reagent e.g. thionyl chloride
  • the transformation comprises a process (forming an invention embodiment, alone or with the immediately or other preceding and following step or steps) which comprises reacting a compound of the formula (I), especially (I-a), wherein Ra is a carboxyl protecting group or especially C 1 -C 6 -alkyl, e.g.
  • This reaction preferably takes place e.g. under the conditions of the Ellman reaction (cf. e.g. F. Chemla, F. Ferreira, J. Org. Chem. 2004, 69, 8244 ), especially in an appropriate solvent or solvent mixture, e.g. a halogenated hydrocarbon, such as dichloromethane, or an ether, such as tetrahydrofuran, preferably in the presence of a weekly active acidic catalyst, such as pyridinium p-toluenesulfonate, Ti(OiPr) 4 or Ti(OEt) 4 as Lewis acid (cf. e.g. T. Boultwood, D. P. Affron, A. D. Trowbridge, J. A. Bull, J. Org. Chem. 2013, 78, 6632 ), for example at a temperature in the range from 0 to 100 °C, e.g. from 15 to 70 °C.
  • an appropriate solvent or solvent mixture e.
  • the reaction is preferably followed by a method (also, alone or with the immediately or other preceding and following step or steps, forming an invention embodiment) comprising reduction of the compound of the formula (X), especially (X-a), to give a sulfinamide compound of the formula (XI), or a salt thereof, especially of the formula (XI-a), more preferably of formula (XI-aa) or a salt thereof, wherein Rb and Ra are as defined for a compound of the formula (X) above, in the presence of a reducing agent.
  • a reducing agent preferably hydrogen in the presence of a catalyst such as Ru, e.g. under transfer catalysis conditions with e.g. isopropanol as solvent at a preferred temperature of 40-90°C, a complex hydride, such as an alkali metal borohydride, e.g. sodium borohydride (if desired in the presence of an acid, e.g. boric acid, toluene sulfonic acid or benzoic acid), an alkali metal aluminium hydride, such as lithium aluminium hydride is used.
  • a catalyst such as Ru
  • a complex hydride such as an alkali metal borohydride, e.g. sodium borohydride (if desired in the presence of an acid, e.g. boric acid, toluene sulfonic acid or benzoic acid)
  • an alkali metal aluminium hydride such as lithium aluminium hydride
  • the reaction preferably takes place in a customary solvent, e.g. an ether, such as diethylether or tetrahydrofuran, and at appropriate temperatures, e.g. in the range from - 78°C to 40°C, e.g. from -78°C to 20°C.
  • a customary solvent e.g. an ether, such as diethylether or tetrahydrofuran
  • diisobutylaluminium hydride, borane, L-Selectride (lithium tri-sec.butyl(hydrido)borate-(1-)) or LiBEt 3 H (lithiumtriethylborohydride) may be used in an appropriate solvent, such as an ether, e.g. tetrahydrofurane, at low temperatures, e.g. in the range from -100 to 0°C, e.g. at -78°C.
  • an ether e.g. tetrahydrofurane
  • the compound of the formula (XI), especially (XI-a), can then be further reacted to result in a compound of the formula (VIII), especially (VIII-a), or a salt thereof:
  • the sulfinamide compound of the formula (XI), or a salt thereof, especially of the formula (XI-a), or a salt thereof wherein Rb is as defined for a compound of the formula (X) and Ra is selected from hydrogen, a carboxyl protecting group, and C 1 -C 6 -alkyl, preferably ethyl or hydrogen, more preferably ethyl, is hydrolyzed in the presence of an acid.
  • a suitable acid is an organic, such as acetic acid or citric acid, or preferably an inorganic acid, e.g. a hydrohalic acid, such as hydrochloric acid, sulfuric acid, phosphoric acid or a mixture of two or more thereof, preferably in aqueous solution in particular comprising a further organic solvent, such as an alcohol, e.g. methanol, ethanol or isopropanol, or an ether, e.g. 1,4-dioxane, tetrahydrofurane, tert-butyl methyl ether or diethylether, or mixtures of two or more of these solvents, preferably at temperatures in the range e.g. from 0 to 60°C, e.g. from 20 to 25°C, or up to reflux.
  • a hydrohalic acid such as hydrochloric acid, sulfuric acid, phosphoric acid or a mixture of two or more thereof
  • a further organic solvent such as an alcohol, e.g.
  • a salt of a compound of the formula (VIII), especially (VIII-a) can then be converted into the free compound of the formula (VIII), especially (VIII-a), using customary processes, or into a different salt, e.g. using ion exchange chromatography, neutralization with a base, e.g. an alkali metal hydroxide, or the like.
  • Compound (XI), especially compound (XI-a), can alternatively be transformed into compound (VIII), especially compound (VIII-a), wherein Ra is C 1 -C 6 -alkyl, preferably ethyl, in the absence of water, e.g. by use of an anhydrous inorganic acid, e.g. a hydrohalic acid, such as hydrochloric acid, either used as gas or generated in situ using a suitable reagent, in the presence of an alcohol of the formula Ra-OH wherein Ra is selected from a carboxyl protecting group, and C 1 -C 6 -alkyl, especially ethyl, respectively.
  • an anhydrous inorganic acid e.g. a hydrohalic acid, such as hydrochloric acid
  • the reaction preferably takes place in a customary solvent, such as an alcohol, e.g. methanol or ethanol, and at appropriate temperatures, e.g. in the range from 0 to 50°C, e.g. from 10 to 30°C.
  • a customary solvent such as an alcohol, e.g. methanol or ethanol
  • the asymmetric mono-oxidation is preferably conducted in the presence of a chiral ligand, e.g. a benzo-cyclopentanolimine named (1 S ,2 R )-1-[(2-hydroxy-3,5-di- tert -butyl-benzylidene)-amino]-indan-2-ol of the formula or other ligands selected from those of the following formula: wherein R 1 is hydrogen, tert -butyl or methoxy; and R 2 is tert -butyl, isopropyl, benzyl or phenyl.
  • a chiral ligand e.g. a benzo-cyclopentanolimine named (1 S ,2 R )-1-[(2-hydroxy-3,5-di- tert -butyl-benzylidene)-amino]-indan-2-ol of the formula or other ligands selected from those of the following formula: wherein R 1 is hydrogen
  • the mono-oxidation is preferably achieved with a peroxide, especially hydrogen peroxide, in an appropriate solvent, such as a ketone, e.g. acetone, e.g. under conditions described by Ellman et al. (cf. D. J. Weix, J. A. Ellman, Org. Lett. 2003, 5, 1317 ) in the presence of a vanadyl complex, especially vanadyl- bis -acetylacetonate, in an appropriate solvent or solvent mixture, e.g. a ketone, such as acetone, preferably at lower temperatures e.g. in the range from -20 to 20°C, e.g. from -5 to 5°C.
  • a peroxide especially hydrogen peroxide
  • an appropriate solvent such as a ketone, e.g. acetone
  • the resulting sulfinylsulfide compound of the formula (XIII), especially (XIII-a), is then reacted under S N 2 substitution with a metal amide, especially an alkaline metal amide, such as lithium amide, in an appropriate solvent or solvent mixture, e.g. in NH 3 in a cyclic ether, such as tetrahydrofuran, preferably at low temperatures, e.g. in the range from -100 to 0 °C, e.g. from -80 to -50°C, resulting in the sulfinamide of the formula (IX), especially (IX-a).
  • a metal amide especially an alkaline metal amide, such as lithium amide
  • an appropriate solvent or solvent mixture e.g. in NH 3 in a cyclic ether, such as tetrahydrofuran, preferably at low temperatures, e.g. in the range from -100 to 0 °C, e.g. from -80
  • the transformation comprises a process (forming an invention embodiment, alone or with the immediately or other preceding and following step or steps) which comprises reacting a compound of the formula (I), especially (I-a), wherein Ra is hydrogen, or a salt thereof, with hydroxylamine or a salt thereof, yielding a compound of the formula (XVII), especially of formula (XVII-a), wherein Ra is hydrogen, if starting from a compound of the formula (I), especially (I-a), wherein Ra is hydrogen, or a salt thereof.
  • the reaction preferably takes place with a hydroxylamine salt, e.g. hydroxylamine hydrochloride, in an appropriate solvent or solvent mixture, e.g. in an alcohol, such as ethanol, in the presence of a base, e.g. pyridine, at preferred temperatures in the range from 0 to 100°C, e.g. in the range from 20 to 90°C.
  • a hydroxylamine salt e.g. hydroxylamine hydrochloride
  • an appropriate solvent or solvent mixture e.g. in an alcohol, such as ethanol
  • a base e.g. pyridine
  • reaction is preferably followed by a method (also forming a separate invention embodiment) comprising cyclization of the compound of the formula (XVII), especially of formula (XVII-a), to give a cyclic O-acyl oxime compound of formula (XVIII), especially of the formula (XVIII-a).
  • the cyclization preferably takes place in the presence of an acid such as an organic acid, e.g. a sulfonic acid, such as p -toluene sulfonic acid, present in stoichiometric or catalytic amounts, preferably in catalytic amounts, e.g. 0.05 to 0.5 equiv., in an appropriate solvent or solvent mixture, e.g. in toluene, e.g. at elevated temperatures from 50 °C to the reflux temperature of the reaction mixture, such as from 70 to 90°C.
  • an acid such as an organic acid, e.g. a sulfonic acid, such as p -toluene sulfonic acid, present in stoichiometric or catalytic amounts, preferably in catalytic amounts, e.g. 0.05 to 0.5 equiv.
  • an appropriate solvent or solvent mixture e.g. in toluene, e.g. at elevated temperatures from 50 °
  • the compound of the formula (XVIII), especially (XVIII-a) can then be further converted (in a further method according to the invention, either as single step or with the preceding reactions above) by a process comprising the reduction of the cyclic O-acyl oxime to provide a compound of formula (VIII), especially of the formula (VIII-a), or a salt thereof, respectively, wherein Ra is hydrogen or a carboxyl protecting group or especially C 1 -C 6 -alkyl, more especially ethyl.
  • Reduction of the cyclic oxime derivatives to the corresponding amines is generally achieved using metal-catalyzed hydrogenation reactions (e.g. Pd or Rh on solid supports like activated carbon or aluminum oxide or Raney-Ni) or complex hydrides (e.g. sodium borohydride or lithium aluminum hydride).
  • the alternative direct reduction of the free oxime of formula (XVII), preferably of formula (XVII-a) to the corresponding amines of formula (VIII) can be generally achieved using metal-catalyzed hydrogenation reactions (e.g. Pd or Rh on solid supports like activated carbon or aluminum oxide or Raney-Ni) or complex hydrides (e.g. sodium borohydride or lithium aluminum hydride).
  • metal-catalyzed hydrogenation reactions e.g. Pd or Rh on solid supports like activated carbon or aluminum oxide or Raney-Ni
  • complex hydrides e.g. sodium borohydride or lithium aluminum hydride
  • the reduction can be achieved using hydrogen in the presence of a transition metal catalyst, such as RhCI(PPh3)3, [Ru(S)-BINAP( p -cymene)CI]CI, Pd/C or Pt/C (if desired in the presence of an acid, e.g. benzoic acid), e.g. under elevated pressure up to 30 bar, in an appropriate solvent or solvent mixture, e.g. THF, methanol or THF/water, e.g. at temperatures from 20°C to 80°C, such as from 25 to 70°C; or by reduction in a two-step approach, consisting of (1) reduction of the C-N double bond using a hydrogenating agent, e.g.
  • a transition metal catalyst such as RhCI(PPh3)3, [Ru(S)-BINAP( p -cymene)CI]CI, Pd/C or Pt/C
  • an acid e.g. benzoic acid
  • an appropriate solvent or solvent mixture e.g. THF,
  • a catalyst e.g. a noble metal catalyst with or without carrier, such as Pt or Pd or Pd or Pt on charcoal, e.g. in an appropriate solvent, such as an alcohol, e.g. methanol or ethanol, at temperatures e.g. in the range from 0°C to reflux temperature, e.g. from 20 to 50°C.
  • the transformation comprises a process (forming an invention embodiment, alone or with the immediately or other preceding and following step or steps) which comprises reacting a compound of the formula (I), especially (I-a), wherein Ra is hydrogen, or a salt thereof, with ammonia or an ammonium salt, yielding a compound of the formula (XIX), especially (XIX-a).
  • the compound may also be present as a mixture of double bond isomers in any relative ratio.
  • the reaction preferably takes place with an ammonium salt, e.g. ammonium acetate, in an appropriate solvent or solvent mixture, e.g. in an organic acid, such as acetic acid, at preferred temperatures in the range from 50 to 150°C, e.g. in the range from 100 to 140°C.
  • an ammonium salt e.g. ammonium acetate
  • an appropriate solvent or solvent mixture e.g. in an organic acid, such as acetic acid
  • the reaction is preferably followed by a method comprising reduction of the compound of the formula (XIX), especially (XIX-a), to give a cyclic lactam compound of the formula (XX), especially of the formula (XX-a).
  • the reduction preferably takes place using hydrogen in the presence of a transition metal catalyst, such as Pd/C, e.g. under elevated pressure up to 10 bar, e.g. in the range 3-8 bar, in an appropriate solvent or solvent mixture, e.g. methanol, e.g. at ttemperatures from 20°C to 150°C, such as from 80 to 140°C.
  • a transition metal catalyst such as Pd/C
  • solvent or solvent mixture e.g. methanol
  • Other possible reduction methods comprise the reduction of the C-N double bond using a hydrogenating agent other than hydrogen, e.g. a hydride salt, such as lithium aluminum hydride. (see e.g. ( Chem. Ber. 1971, 104, 2134-2142 ).
  • Compound (XX), especially compound (XX-a), can then be converted to compound (VIII), especially to compound (VIII-a), as described, for example, in WO 2008/083967A , especially Examples 7 and 13 therein.
  • an inorganic acid e.g. sulfuric acid or hydrochloric acid
  • an organic acid e.
  • the transformation comprises a process (forming an invention embodiment, alone or with the immediately or other preceding and following step or steps) which comprises reacting a compound of the formula (I), especially (I-a), wherein Ra is hydrogen or a carboxyl protecting group or especially C 1 -C 6 -alkyl, more especially ethyl, with an O-substituted hydroxylamine selected from or in each case a salt thereof, wherein Rc and Rd are independently selected from C 1 -C 6 -alkyl, C 6 -C 10 -aryl, C 6 -C 10 -aryl-C 1 -C 6 -alkyl, C 3 -C 8 -cycloalkyl, C 3 -C 8 -cycloalkyl-C 1 -C 6 -alkyl, heterocyclyl or heterocyclyl-C 1 -C 6 -alkyl, wherein said heterocyclyl is a mono- or polycyclic, unsaturated, partially saturated, saturated or aromatic
  • the reaction with the O-substituted hydroxylamine preferably takes place in the presence of an acid such as an organic acid, e.g. a sulfonic acid, such as p -toluene sulfonic acid, present in stoichiometric or catalytic amounts, preferably in catalytic amounts, e.g. 0.05 to 0.5 equiv., in an appropriate solvent or solvent mixture, e.g. in toluene, e.g. at elevated temperatures from 50 °C to the reflux temperature of the reaction mixture, such as from 70 to 90°C.
  • an acid such as an organic acid, e.g. a sulfonic acid, such as p -toluene sulfonic acid, present in stoichiometric or catalytic amounts, preferably in catalytic amounts, e.g. 0.05 to 0.5 equiv.
  • an appropriate solvent or solvent mixture e.g. in toluene,
  • the substituents Rc and Rd are preferably selected in a way to avoid cyclization of the compound of formula (XVII***) to a compound of the formula (XVIII) (see SCHEME 8), and/or - if desired - can be removed to yield the corresponding compound wherein A is hydroxyl, i.e. a compound of formula (XVII).
  • the compound of formula (XVII***) Ra can have one of the meanings mentioned other than hydrogen.
  • the compound of the formula (XVII***), especially (XVII***-a) can then be further converted (in a further method according to the invention, either as single step or with the preceding reactions above) by a process comprising the reduction of the O-substituted oxime group to provide a compound of formula (VIII), especially of the formula (VIII-a), or a salt thereof, respectively, wherein Ra is hydrogen or a carboxyl protecting group or especially C 1 -C 6 -alkyl, more especially ethyl.
  • Reduction of the oximes derivatives to the corresponding amines is generally achieved using metal-catalyzed hydrogenation reactions (e.g. Pd or Rh on solid supports like activated carbon or aluminum oxide or Raney-Ni) or complex hydrides (e.g. sodium borohydride or lithium aluminum hydride).
  • the reduction can be achieved using hydrogen in the presence of a transition metal catalyst, such as RhCI(PPh3)3, [Ru(S)-BINAP(p-cymene)CI]CI, Pd/C or Pt/C (if desired in the presence of an acid, e.g. benzoic acid), e.g. under elevated pressure up to 30 bar, in an appropriate solvent or solvent mixture, e.g. THF, methanol or THF/water, e.g. at temperatures from 20°C to 80°C, such as from 25 to 70°C; or by reduction in a two-step approach, consisting of (1) reduction of the C-N double bond using a hydrogenating agent, e.g.
  • a transition metal catalyst such as RhCI(PPh3)3, [Ru(S)-BINAP(p-cymene)CI]CI, Pd/C or Pt/C
  • an acid e.g. benzoic acid
  • an appropriate solvent or solvent mixture e.g. THF, methanol or T
  • a catalyst e.g. a noble metal catalyst with or without carrier, such as Pt or Pd or Pd or Pt on charcoal, e.g. in an appropriate solvent, such as an alcohol, e.g. methanol or ethanol, at temperatures e.g. in the range from 0°C to reflux temperature, e.g. from 20 to 50°C.
  • the intermediates in particular the novel compounds of formula (XV), in particular of formula (I) or (II), and of formula (XVI), in particular of formula (X), (XVII), (XVII*), (XVII**), (XVIII), and (IX), or the preferred stereoisomer thereof, as depicted above, and the products of the process of the present invention can be used in the synthesis of NEP inhibitors or salts or pro-drugs thereof, in particular they can be used in the synthesis of NEP inhibitors comprising a ⁇ -amino- ⁇ -biphenyl- ⁇ -methylalkanoic acid, or acid ester, backbone.
  • NEP inhibitor describes a compound which inhibits the activity of the enzyme neutral endopeptidase (NEP, EC 3.4.24.11).
  • a compound according to formula (VIII), preferably of formula (VIII-a), more preferably of formula (VIII-aa), or salt thereof is further reacted to obtain the NEP inhibitor pro-drug of formula (10) preferably of formula (10-a) wherein R1 is hydrogen or C 1 -C 6 -alkyl, preferably ethyl, by reaction with succinic acid or a derivative thereof, preferably succinic acid anhydride.
  • the ester can be saponified to the free acid providing the NEP inhibitor drug compound.
  • the compound of formula (10-a) is N -(3-carboxy-1-oxopropyl)-(4 S )-( p- phenylphenylmethyl)-4-amino-(2 R )-methylbutanoic acid ethyl ester (known in the art as AHU377) or a salt thereof.
  • NEP inhibitor pro-drug N -(3-carboxy-1-oxopropyl)-(4 S )-( p -phenylphenylmethyl)-4-amino-(2 R )-methylbutanoic acid ethyl ester optionally is further reacted to obtain the NEP inhibitor N -(3-carboxy-1-oxopropyl)-(4 S )-( p -phenylphenylmethyl)-4-amino-(2 R )-methylbutanoic acid.
  • the invention also relates to novel compounds mentioned above and below. From these compounds, those mentioned specifically in the Examples are most preferred.
  • the reaction mixture was concentrated under reduced pressure to remove acetone and the residue was diluted with water and extracted with ethyl acetate. The combined organic layer was washed with water and brine, dried over anhydrous Na 2 SO 4 and concentrated under reduced pressure to get the crude product 6 as wine red color viscous oil (159 g).
  • reaction mixture was concentrated under reduced pressure and the residue was diluted with water and extracted with ethyl acetate. The combined organic layer was washed with water and brine, dried over anhydrous Na 2 SO 4 and concentrated under reduced pressure to obtain the crude product.
  • the crude product obtained was purified by column chromatography (two times) (silica gel, 230-400 mesh; eluent: 0-35% ethyl acetate in petroleum ether) and then the solid obtained was washed with petroleum ether and dried to get 5-([1,1'-biphenyl]-4-yl)-2-methyl-4-oxopentanoic acid 7 as white solid (55.3 g, 47.1%).
  • Example 5 Step-5 - manufacture of 5-([1,1'-biphenyl]-4-yl)-2-methyl-4-oxopentanoic acid ethyl ester 7B
  • reaction mixture was concentrated under reduced pressure to remove the volatiles and the residue was diluted with water and extracted with ethyl acetate. The combined organic layer was washed with water and brine, dried over anhydrous Na 2 SO 4 and concentrated under reduced pressure to obtain the crude product.
  • Compound 12 is commercially available and can be prepared according to literature procedures (e.g. Adv. Synth. Catal. 2003, 345, 308-322 ; Adv. Synth. Catal. 2004, 346, 1263-1266 ; Chem. Eur. J. 2009, 15, 3983-4010 ; Chem. Eur. J. 2012, 18, 14267-14271 ) from 4-methoxy-2-methylene-4-oxobutanoic acid 11.
  • Ammonium acetate (1.45 g, 18.81 mmol) was added to a solution of 5-([1,1'-biphenyl]-4-yl)-2-methyl-4-oxopentanoic acid 7 (242 mg, 0.857 mmol) in a mixture of THF and water (1:1 V/V, 2.5 mL) at room temperature.
  • the yellow suspension was treated with sodium cyanoborohydride (58 mg, 0.923 mmol) and stirred at 40 °C over night.
  • Aqueous 6 M hydrochloric acid (5 mL) was added slowly to control the gas evolution, followed by addition of ethyl acetate (8 mL). The phases were separated, and the organic layer was concentrated under reduced pressure.
  • reaction mixture was further stirred at room temperature for 1 h, then cooled to 0 °C.
  • Aqueous 3 M hydrochloric acid (20 mL) was added, and the phases were separated.
  • the organic layer was dried over Na 2 SO 4 , filtered and concentratec in vacuo.
  • the residue was dissolved in ethanol (3 mL), followed by addition of aqueous 6 M hydrochloric acid (2 mL).
  • the reaction mixture was subsequently heated to 120 °C in the microwave for 30 min. After concentration in vacuo, the residue was taken up in water and dichloromethane.
  • the transaminase ATA-447 (30 mg; Codexis Inc., Redwood City, CA, USA) was dissolved in 200 ⁇ L of the buffer solution and shaken for 5 min, then added to the reaction mixture. The reaction mixture was shaken at 50 °C and 180 rpm for 5 d, providing 78% conversion to the desired product B with > 99:1 dr.
  • Ammonium acetate (3.32 g, 43.07 mmol) was added to a solution of 5-([1,1'-biphenyl]-4-yl)-2-methyl-4-oxopentanoic acid (1.52 g, 5.38 mmol) in acetic acid (18 mL) to provide a suspension in a pressure-resistant microwave vial which was immediately sealed.
  • the vial was placed in an oil bath at room temperature and was subsequently heated to 130 °C. After 17 h, the vial was cooled back to room temperature prior to opening, and the yellow solution was separated between methylene dichloride (30 mL) and 0.1 M dipotassium hydrogen phosphate solution (30 mL of a solution of 1.74 g in 100 mL water).
  • the aqueous phase was washed with methylene dichloride and the combined organic phases extracted twice with 1 M dipotassium hydrogen phosphate solution (30 mL of a solution of 17.4 g in 100 mL water).
  • the organic phase was dried over solid sodium sulphate and the solvent stripped by rotary evaporation to give a yellow solid (1.35 g, 95% crude yield).

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Catalysts (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Nitrogen And Oxygen As The Only Ring Hetero Atoms (AREA)
  • Pyrrole Compounds (AREA)

Claims (13)

  1. Composé de formule (XV), ou sel correspondant
    Figure imgb0241
     préférablement de formule (XV-a)
    Figure imgb0242
    R5 étant choisi parmi hydrogène et un groupe -CO-OR*, et
    Ra et R* étant, indépendamment l'un de l'autre, choisis parmi hydrogène, un groupe protecteur de carboxyle, et C1-C6-alkyle, préférablement éthyle, le groupe protecteur de carboxyle étant choisi parmi C6-C10-aryl-C1-C6-alkyle et SiR11R12R13, R11, R12, et R13 étant, indépendamment l'un de l'autre, C1-C7-alkyle, C6-C10-aryle ou phényl-C1-C4-alkyle.
  2. Composé de formule (XV) selon la revendication 1,
    a) le composé étant de formule (I), ou un sel correspondant ;
    Figure imgb0243
     préférablement de formule (I-a), ou un sel correspondant ;
    Figure imgb0244
     Ra étant choisi parmi hydrogène, un groupe protecteur de carboxyle, et C1-C6-alkyle, préférablement éthyle, le groupe protecteur de carboxyle étant choisi parmi C6-C10-aryl-C1-C6-alkyle et SiR11R12R13, R11, R12, et R13 étant, indépendamment l'un de l'autre, C1-C7-alkyle, C6-C10-aryle ou phényl-C1-C4-alkyle ou
    b) le composé étant de formule (II), ou un sel correspondant ;
    Figure imgb0245
     préférablement de formule (II-a), ou un sel correspondant ;
    Figure imgb0246
     Ra et R* étant, indépendamment l'un de l'autre, choisis parmi hydrogène, un groupe protecteur de carboxyle, et C1-C6-alkyle, préférablement éthyle, le groupe protecteur de carboxyle étant choisi parmi C6-C10-aryl-C1-C6-alkyle et SiR11R12R13, R11, R12, et R13 étant, indépendamment l'un de l'autre, C1-C7-alkyle, C6-C10-aryle ou phényl-C1-C4-alkyle.
  3. Composé de formule (XVI), ou sel correspondant
    Figure imgb0247
     préférablement de formule (XVI-a)
    Figure imgb0248
    R6 étant -O-Ra, et R7 étant choisi parmi
    • -S(=O)-Rb
    • -OH
    • A, A étant -O-C(=O)-Rc ou -O-Rd
    Ra étant choisi parmi hydrogène, un groupe protecteur de carboxyle, et C1-C6-alkyle, préférablement éthyle, le groupe protecteur de carboxyle étant choisi parmi C6-C10-aryl-C1-C6-alkyle et SiR11R12R13, R11, R12, et R13 étant, indépendamment l'un de l'autre, C1-C7-alkyle, C6-C10-aryle ou phényl-C1-C4-alkyle et
    Rb, Rc et Rd étant indépendamment choisis parmi C1-C6-alkyle, C6-C10-aryle, C6-C10-aryl-C1-C6-alkyle, C3-C8-cycloalkyle, C3-C8-cycloalkyl-C1-C6-alkyle, hétérocyclyle et hétérocyclyl-C1-C6-alkyle, ledit hétérocyclyle étant un système cyclique monocyclique ou polycyclique, insaturé, partiellement saturé, saturé ou aromatique comportant 5 à 14 atomes de cycle et comportant un ou plusieurs hétéroatomes indépendamment choisis parmi azote, oxygène, soufre, S(=O) -et S-(=O)2, et chaque groupe aryle ou hétérocyclyle pouvant éventuellement être substitué par un, deux ou trois substituants indépendamment choisis parmi halogéno, C1-C7-alkyle, halogéno-C1-C7-alkyle, et C1-C7-alcoxy, halogéno-C1-C7-alcoxy, et C1-C7-alcoxy-C1-C7-alkyle, ou
    R6 et R7 représentant ensemble -O- ou formant une liaison.
  4. Composé de formule (XVI) selon la revendication 3,
    a) le composé étant de formule (X), ou un sel correspondant ;
    Figure imgb0249
     préférablement de formule (X-a),
    Figure imgb0250
    Ra étant choisi parmi hydrogène, un groupe protecteur de carboxyle, et C1-C6-alkyle, préférablement éthyle, le groupe protecteur de carboxyle étant choisi parmi C6-C10-aryl-C1-C6-alkyle et SiR11R12R13, R11, R12, et R13 étant, indépendamment l'un de l'autre, C1-C7-alkyle, C6-C10-aryle ou phényl-C1-C4-alkyle,
    Rb étant choisi parmi C1-C6-alkyle, C6-C10-aryle, C6-C10-aryl-C1-C6-alkyle, C3-C8-cycloalkyle, C3-C8-cycloalkyl-C1-C6-alkyle, hétérocyclyle ou hétérocyclyl-C1-C6-alkyle, ledit hétérocyclyle étant un système cyclique monocyclique ou polycyclique, insaturé, partiellement saturé, saturé ou aromatique comportant 5 à 14 atomes de cycle et comportant un ou plusieurs hétéroatomes indépendamment choisis parmi azote, oxygène, soufre, S(=O)- et S-(=O)2, et chaque groupe aryle ou hétérocyclyle pouvant éventuellement être substitué par un, deux ou trois substituants indépendamment choisis parmi halogéno, C1-C7-alkyle, halogéno-C1-C7-alkyle, et C1-C7-alcoxy, halogéno-C1-C7-alcoxy, et C1-C7-alcoxy-C1-C7-alkyle,
    b) le composé étant de formule (XVII), ou un sel correspondant
    Figure imgb0251
     préférablement de formule (XVII-a),
    Figure imgb0252
     Ra étant choisi parmi hydrogène, un groupe protecteur de carboxyle, et C1-C6-alkyle, préférablement éthyle, le groupe protecteur de carboxyle étant choisi parmi C6-C10-aryl-C1-C6-alkyle et SiR11R12R13, R11, R12, et R13 étant, indépendamment l'un de l'autre, C1-C7-alkyle, C6-C10-aryle ou phényl-C1-C4-alkyle,
    c) le composé étant de formule (XVII*),
    Figure imgb0253
     préférablement de formule (XVII*-a),
    Figure imgb0254
    Ra étant choisi parmi hydrogène, un groupe protecteur de carboxyle, et C1-C6-alkyle, préférablement éthyle, le groupe protecteur de carboxyle étant choisi parmi C6-C10-aryl-C1-C6-alkyle et SiR11R12R13, R11, R12, et R13 étant, indépendamment l'un de l'autre, C1-C7-alkyle, C6-C10-aryle ou phényl-C1-C4-alkyle,
    Rc étant choisi parmi C1-C6-alkyle, C6-C10-aryle, C6-C10-aryl-C1-C6-alkyle, C3-C8-cycloalkyle, C3-C8-cycloalkyl-C1-C6-alkyle, hétérocyclyle ou hétérocyclyl-C1-C6-alkyle, ledit hétérocyclyle étant un système cyclique monocyclique ou polycyclique, insaturé, partiellement saturé, saturé ou aromatique comportant 5 à 14 atomes de cycle et comportant un ou plusieurs hétéroatomes indépendamment choisis parmi azote, oxygène, soufre, S(=O)- et S-(=O)2, et chaque groupe aryle ou hétérocyclyle pouvant éventuellement être substitué par un, deux ou trois substituants indépendamment choisis parmi halogéno, C1-C7-alkyle, halogéno-C1-C7-alkyle, et C1-C7-alcoxy, halogéno-C1-C7-alcoxy, et C1-C7-alcoxy-C1-C7-alkyle,
    d) le composé étant de formule (XVII**),
    Figure imgb0255
     préférablement de formule (XVII**-a),
    Figure imgb0256
    Ra étant choisi parmi hydrogène, un groupe protecteur de carboxyle, et C1-C6-alkyle, préférablement éthyle, le groupe protecteur de carboxyle étant choisi parmi C6-C10-aryl-C1-C6-alkyle et SiR11R12R13, R11, R12, et R13 étant, indépendamment l'un de l'autre, C1-C7-alkyle, C6-C10-aryle ou phényl-C1-C4-alkyle,
    Rd étant choisi parmi C1-C6-alkyle, C6-C10-aryle, C6-C10-aryl-C1-C6-alkyle, C3-C8-cycloalkyle, C3-C8-cycloalkyl-C1-C6-alkyle, hétérocyclyle ou hétérocyclyl-C1-C6-alkyle, ledit hétérocyclyle étant un système cyclique monocyclique ou polycyclique, insaturé, partiellement saturé, saturé ou aromatique comportant 5 à 14 atomes de cycle et comportant un ou plusieurs hétéroatomes indépendamment choisis parmi azote, oxygène, soufre, S(=O)- et S-(=O)2, et chaque groupe aryle ou hétérocyclyle pouvant éventuellement être substitué par un, deux ou trois substituants indépendamment choisis parmi halogéno, C1-C7-alkyle, halogéno-C1-C7-alkyle, et C1-C7-alcoxy, halogéno-C1-C7-alcoxy, et C1-C7-alcoxy-C1-C7-alkyle,
    e) le composé étant de formule (XVIII),
    Figure imgb0257
     préférablement de formule (XVIII-a)
    Figure imgb0258
     ou
    f) le composé étant de formule (XIX),
    Figure imgb0259
     préférablement de formule (XIX-a)
    Figure imgb0260
  5. Procédé pour la préparation d'un composé de formule (I), ou d'un sel correspondant ;
    Figure imgb0261
     préférablement de formule (I-a), ou d'un sel correspondant ;
    Figure imgb0262
     Ra étant choisi parmi hydrogène, un groupe protecteur de carboxyle et C1-C6-alkyle, préférablement éthyle,
    comprenant la mise en réaction d'un composé de formule (II), ou d'un sel correspondant
    Figure imgb0263
     préférablement de formule (II-a), ou d'un sel correspondant ;
    Figure imgb0264
     Ra et R* étant, indépendamment l'un de l'autre, choisis parmi hydrogène, un groupe protecteur de carboxyle, et C1-C6-alkyle, préférablement éthyle, dans - si nécessaire - des conditions de réaction de déprotection, suivies par des conditions de réaction de décarboxylation, et éventuellement par l'introduction d'un fragment Ra choisi parmi un groupe protecteur de carboxyle et C1-C6-alkyle, pour fournir le composé de formule (I).
  6. Procédé selon la revendication 5, le composé de formule (II), ou un sel correspondant
    Figure imgb0265
     préférablement de formule (II-a), ou un sel correspondant ;
    Figure imgb0266
     Ra et R* étant, indépendamment l'un de l'autre, choisis parmi hydrogène, un groupe protecteur de carboxyle, et C1-C6-alkyle, préférablement éthyle, étant préparé par un procédé comprenant la mise en réaction d'un composé de formule (III),
    Figure imgb0267
     R* étant choisi parmi un groupe protecteur de carboxyle et C1-C6-alkyle, préférablement éthyle, avec un dérivé de propionate de formule (IV),
    Figure imgb0268
     préférablement de formule (IV-a),
    Figure imgb0269
     X étant un groupe partant et Ra étant choisi parmi un groupe protecteur de carboxyle et C1-C6-alkyle, préférablement éthyle,
    et, si nécessaire, le remplacement des groupes protecteurs de carboxyle R* et Ra par un groupe choisi parmi hydrogène et C1-C6-alkyle, pour fournir le composé de formule (II).
  7. Procédé selon la revendication 6, le composé de formule (III),
    Figure imgb0270
     R* étant choisi parmi un groupe protecteur de carboxyle et C1-C6-alkyle, préférablement éthyle, étant préparé par un procédé comprenant la mise en réaction d'un composé de formule (V),
    Figure imgb0271
     ou d'un dérivé réactif correspondant,
    avec un sel d'un demi-ester d'acide malonique de formule (VI),
    Figure imgb0272
     R* étant choisi parmi un groupe protecteur de carboxyle et C1-C6-alkyle, préférablement éthyle.
  8. Procédé pour la préparation d'un composé de formule (I), ou d'un sel correspondant ;
    Figure imgb0273
     préférablement de formule (I-a), ou d'un sel correspondant ;
    Figure imgb0274
     Ra étant choisi parmi hydrogène, un groupe protecteur de carboxyle et C1-C6-alkyle, préférablement éthyle,
    comprenant la mise en réaction d'un dérivé dianionique activé du composé de formule (V), ou d'un sel correspondant
    Figure imgb0275
     avec un composé de formule (XIV), ou un sel correspondant
    Figure imgb0276
     préférablement de formule (XIV-a)
    Figure imgb0277
     Rf étant choisi parmi
    - -O-R*, R* étant choisi parmi un groupe protecteur de carboxyle et C1-C6-alkyle, préférablement méthyle,
    - -N(CH3)-O(CH3),
    - morpholinyle, et
    - imidazolinyle,
    en présence d'une base, et
    suivie par une réaction de décarboxylation,
    pour obtenir le composé de formule (I), ou un sel correspondant, Ra étant hydrogène,
    éventuellement suivie par la mise en réaction du composé de formule (I) obtenu, ou d'un sel correspondant, Ra étant hydrogène, avec un agent introduisant un groupe protecteur de carboxyle, pour fournir le composé de formule (I), Ra étant un groupe protecteur de carboxyle, et/ou éventuellement suivie par la mise en réaction du composé de formule (I), ou d'un sel correspondant, Ra étant hydrogène, avec un réactif de couplage en présence d'un C1-C6-alkanol, notamment d'éthanol, pour fournir le composé de formule (I), Ra étant C1-C6-alkyle, préférablement éthyle.
  9. Procédé selon la revendication 7 ou 8, le composé de formule (V),
    Figure imgb0278
     étant préparé par un procédé comprenant l'hydrolyse d'un nitrile de formule (VII)
    Figure imgb0279
  10. Procédé pour la préparation d'un composé de formule (VIII), ou d'un sel correspondant
    Figure imgb0280
     préférablement de formule (VIII-a), ou d'un sel correspondant
    Figure imgb0281
     plus préférablement de formule (VIII-aa), ou d'un sel correspondant
    Figure imgb0282
    Ra étant choisi parmi hydrogène et C1-C6-alkyle, préférablement éthyle, et
    Re étant choisi parmi hydrogène et un groupe protecteur d'azote,
    par un procédé comprenant
    (i) la mise en réaction d'un composé de formule (I), ou d'un sel correspondant ;
    Figure imgb0283
     préférablement de formule (I-a), ou d'un sel correspondant ;
    Figure imgb0284
     Ra étant hydrogène,
    avec de l'ammoniac, une amine primaire ou secondaire, ou des sels correspondants, pour fournir un composé de formule (VIII), préférablement le composé de formule (VIII-a), plus préférablement de formule (VIII-aa), Ra et Re étant hydrogène, ou un sel correspondant, ou
    (ii) la conversion d'un composé de formule (I), ou d'un sel correspondant ;
    Figure imgb0285
     préférablement de formule (I-a), ou d'un sel correspondant ;
    Figure imgb0286
     Ra étant choisi parmi hydrogène et C1-C6-alkyle, préférablement hydrogène, en composé de formule (VIII), préférablement en composé de formule (VIII-a), plus préférablement de formule (VIII-aa), Ra étant choisi parmi hydrogène et C1-C6-alkyle, préférablement hydrogène, et Re étant hydrogène, ou en un sel correspondant, en le mettant en contact avec une ω-transaminase (S)-sélective en présence d'un donneur de type amine et d'une coenzyme, le taux de conversion du composé de formule (I) en composé de formule (VIII), préférablement le taux de conversion du composé de formule (I-a) en composé de formule (VIII-a), plus préférablement de formule (VIII-aa), étant supérieur à 50%, ou
    (iii)
    a) la mise en réaction d'un composé de formule (I), ou d'un sel correspondant ;
    Figure imgb0287
     préférablement de formule (I-a), ou d'un sel correspondant ;
    Figure imgb0288
     Ra étant choisi parmi un groupe protecteur de carboxyle et C1-C6-alkyle, préférablement éthyle, avec un composé de type aminosulfinyle de formule (IX), ou un sel correspondant,
    Figure imgb0289
     notamment de formule (IX-a),
    Figure imgb0290
     Rb étant choisi parmi C1-C6-alkyle, C6-C10-aryle, C6-C10-aryl-C1-C6-alkyle, C3-C8-cycloalkyle, C3-C8-cycloalkyl-C1-C6-alkyle, hétérocyclyle ou hétérocyclyl-C1-C6-alkyle, ledit hétérocyclyle étant un système cyclique monocyclique ou polycyclique, insaturé, partiellement saturé, saturé ou aromatique comportant 5 à 14 atomes de cycle et comportant un ou plusieurs hétéroatomes indépendamment choisis parmi azote, oxygène, soufre, S(=O)- et S-(=O)2, et chaque groupe aryle ou hétérocyclyle pouvant éventuellement être substitué par un, deux ou trois substituants indépendamment choisis parmi halogéno, C1-C7-alkyle, halogéno-C1-C7-alkyle, et C1-C7-alcoxy, halogéno-C1-C7-alcoxy, et C1-C7-alcoxy-C1-C7-alkyle, pour donner un composé de type sulfinimide de formule (X), ou un sel correspondant
    Figure imgb0291
     préférablement de formule (X-a),
    Figure imgb0292
     Rb étant choisi parmi C1-C6-alkyle, C6-C10-aryle, C6-C10-aryl-C1-C6-alkyle, C3-C8-cycloalkyle, C3-C8-cycloalkyl-C1-C6-alkyle, hétérocyclyle ou hétérocyclyl-C1-C6-alkyle, ledit hétérocyclyle étant un système cyclique monocyclique ou polycyclique, insaturé, partiellement saturé, saturé ou aromatique comportant 5 à 14 atomes de cycle et comportant un ou plusieurs hétéroatomes indépendamment choisis parmi azote, oxygène, soufre, S(=O)- et S-(=O)2, et chaque groupe aryle ou hétérocyclyle pouvant éventuellement être substitué par un, deux ou trois substituants indépendamment choisis parmi halogéno, C1-C7-alkyle, halogéno-C1-C7-alkyle, et C1-C7-alcoxy, halogéno-C1-C7-alcoxy, et C1-C7-alcoxy-C1-C7-alkyle, et
    Ra étant choisi parmi un groupe protecteur de carboxyle et C1-C6-alkyle, préférablement éthyle,
    b) la réduction du composé obtenu de formule (X), ou d'un sel correspondant, en présence d'un agent de réduction pour donner un composé de type sulfinamide de formule (XI), ou un sel correspondant
    Figure imgb0293
     préférablement de formule (XI-a), ou un sel correspondant
    Figure imgb0294
     plus préférablement de formule (XI-aa), ou un sel correspondant
    Figure imgb0295
     Rb étant choisi parmi C1-C6-alkyle, C6-C10-aryle, C6-C10-aryl-C1-C6-alkyle, C3-C8-cycloalkyle, C3-C8-cycloalkyl-C1-C6-alkyle, hétérocyclyle ou hétérocyclyl-C1-C6-alkyle, ledit hétérocyclyle étant un système cyclique monocyclique ou polycyclique, insaturé, partiellement saturé, saturé ou aromatique comportant 5 à 14 atomes de cycle et comportant un ou plusieurs hétéroatomes indépendamment choisis parmi azote, oxygène, soufre, S(=O)- et S-(=O)2, et chaque groupe aryle ou hétérocyclyle pouvant éventuellement être substitué par un, deux ou trois substituants indépendamment choisis parmi halogéno, C1-C7-alkyle, halogéno-C1-C7-alkyle, et C1-C7-alcoxy, halogéno-C1-C7-alcoxy, et C1-C7-alcoxy-C1-C7-alkyle, et
    Ra étant choisi parmi un groupe protecteur de carboxyle et C1-C6-alkyle, préférablement éthyle,
    c) la mise en réaction du composé obtenu de type sulfinamide de formule (XI), ou d'un sel correspondant, par l'hydrolyse du groupe sulfonamide en présence d'un acide pour fournir un composé de formule (VIII), ou un sel correspondant, Ra étant choisi parmi un groupe protecteur de carboxyle et C1-C6-alkyle, préférablement éthyle, et Re étant hydrogène, et
    d) l'élimination - s'il est présent - d'un quelconque groupe protecteur de carboxyle du composé obtenu de formule (VIII), ou d'un sel correspondant, pour fournir un composé de formule (VIII), ou un sel correspondant, Ra étant choisi parmi hydrogène et C1-C6-alkyle, préférablement éthyle, et Re étant hydrogène ou
    (iv)
    a) la mise en réaction d'un composé de formule (I), ou d'un sel correspondant ;
    Figure imgb0296
     préférablement de formule (I-a), ou d'un sel correspondant ;
    Figure imgb0297
     Ra étant hydrogène,
    avec de l'hydroxylamine ou un sel correspondant pour fournir un composé de formule (XVII), ou un sel correspondant
    Figure imgb0298
     préférablement de formule (XVII-a),
    Figure imgb0299
     Ra étant hydrogène,
    b) subséquemment, la cyclisation du composé obtenu de formule (XVII) pour donner le composé correspondant de formule (XVIII)
    Figure imgb0300
     préférablement de formule (XVIII-a)
    Figure imgb0301
    c) la réduction du composé obtenu de formule (XVIII) en présence d'un agent de réduction, pour obtenir le composé de formule (VIII), Ra et Re étant tous deux hydrogène, ou
    (v)
    a) la mise en réaction d'un composé de formule (I), ou d'un sel correspondant ;
    Figure imgb0302
     préférablement de formule (I-a), ou d'un sel correspondant ;
    Figure imgb0303
     Ra étant hydrogène,
    avec de l'ammoniac ou un sel d'ammonium, donnant un composé de formule (XIX)
    Figure imgb0304
     préférablement de formule (XIX-a)
    Figure imgb0305
    b) la réduction du composé obtenu de formule (XIX), notamment (XIX-a), avec un agent de réduction, pour donner un composé de type lactame de formule (XX),
    Figure imgb0306
     préférablement de formule (XX-a)
    Figure imgb0307
     plus préférablement de formule (XX-aa), et
    Figure imgb0308
    c) la mise en réaction du composé obtenu de formule (XX) dans des conditions d'ouverture de cycle, éventuellement en présence d'un C1-C7-alcool, pour fournir le composé de formule (VIII), Ra étant choisi parmi hydrogène et C1-C6-alkyle, préférablement éthyle, et Re étant hydrogène, ou
    (vi)
    a) la mise en réaction d'un composé de formule (I), ou d'un sel correspondant ;
    Figure imgb0309
     préférablement de formule (I-a), ou d'un sel correspondant ;
    Figure imgb0310
     Ra étant choisi parmi hydrogène, un groupe protecteur de carboxyle et C1-C6-alkyle, préférablement éthyle,
    avec une hydroxylamine O-substituée choisie parmi
    Figure imgb0311
     ou dans chaque cas un sel correspondant,
    Rc et Rd étant indépendamment choisis parmi C1-C6-alkyle, C6-C10-aryle, C6-C10-aryl-C1-C6-alkyle, C3-C8-cycloalkyle, C3-C8-cycloalkyl-C1-C6-alkyle, hétérocyclyle et hétérocyclyl-C1-C6-alkyle, ledit hétérocyclyle étant un système cyclique monocyclique ou polycyclique, insaturé, partiellement saturé, saturé ou aromatique comportant 5 à 14 atomes de cycle et comportant un ou plusieurs hétéroatomes indépendamment choisis parmi azote, oxygène, soufre, S(=O)-et S-(=O)2, et chaque groupe aryle ou hétérocyclyle pouvant éventuellement être substitué par un, deux ou trois substituants indépendamment choisis parmi halogéno, C1-C7-alkyle, halogéno-C1-C7-alkyle, et C1-C7-alcoxy, halogéno-C1-C7-alcoxy, et C1-C7-alcoxy-C1-C7-alkyle,
    pour fournir un composé de formule (XVII***),
    Figure imgb0312
     préférablement de formule (XVII-a),
    Figure imgb0313
     Ra étant choisi parmi hydrogène, un groupe protecteur de carboxyle, et C1-C6-alkyle, préférablement éthyle, et
    A étant -O-C(=O)-Rc ou -O-Rd,
    Rc et Rd étant indépendamment choisis parmi C1-C6-alkyle, C6-C10-aryle, C6-C10-aryl-C1-C6-alkyle, C3-C8-cycloalkyle, C3-C8-cycloalkyl-C1-C6-alkyle, hétérocyclyle et hétérocyclyl-C1-C6-alkyle, ledit hétérocyclyle étant un système cyclique monocyclique ou polycyclique, insaturé, partiellement saturé, saturé ou aromatique comportant 5 à 14 atomes de cycle et comportant un ou plusieurs hétéroatomes indépendamment choisis parmi azote, oxygène, soufre, S(=O)- et S-(=O)2, et chaque groupe aryle ou hétérocyclyle pouvant éventuellement être substitué par un, deux ou trois substituants indépendamment choisis parmi halogéno, C1-C7-alkyle, halogéno-C1-C7-alkyle, et C1-C7-alcoxy, halogéno-C1-C7-alcoxy, et C1-C7-alcoxy-C1-C7-alkyle,
    b) la réduction du composé obtenu de formule (XVII***), ou d'un sel correspondant pour fournir le composé de formule (VIII) ou un sel correspondant, Ra étant choisi parmi hydrogène, un groupe protecteur de carboxyle et C1-C6-alkyle, préférablement éthyle, et Re étant hydrogène, et
    c) l'élimination - s'il est présent - d'un quelconque groupe protecteur de carboxyle du composé obtenu de formule (VIII), ou d'un sel correspondant,
    et toutes les variantes de réaction (i) à (vi) étant éventuellement suivies par
    éventuellement la mise en réaction du composé obtenu de formule (VIII), ou d'un sel correspondant, Ra étant choisi parmi hydrogène et C1-C6-alkyle, préférablement éthyle, et Re étant hydrogène, avec un agent introduisant un groupe protecteur d'amino, pour fournir le composé de formule (VIII), Ra étant choisi parmi hydrogène et C1-C6-alkyle, préférablement éthyle, et Re étant un groupe protecteur d'azote, et/ou
    éventuellement suivies par la mise en réaction du composé de formule (VIII), Ra étant hydrogène et Re étant choisi parmi hydrogène et un groupe protecteur d'azote, avec un réactif de couplage en présence d'un C1-C6-alkanol, notamment l'éthanol, pour fournir le composé de formule (VIII), Ra étant C1-C6-alkyle, préférablement éthyle, et Re étant un groupe protecteur d'azote.
  11. Procédé selon la revendication 9, le composé de formule (I) étant obtenu par un procédé selon l'une quelconque des revendications 5 à 8.
  12. Procédé selon la revendication 10 ou 11, le composé obtenu de formule (VIII) ou un sel correspondant
    Figure imgb0314
     préférablement de formule (VIII-a), ou un sel correspondant
    Figure imgb0315
     plus préférablement de formule (VIII-aa), ou un sel correspondant
    Figure imgb0316
     Ra étant choisi parmi hydrogène et éthyle, et Re étant choisi parmi hydrogène et un groupe protecteur d'azote,
    étant converti en un composé de formule (10)
    Figure imgb0317
     préférablement de formule (10-a)
    Figure imgb0318
     R1 étant hydrogène ou C1-C6-alkyle, préférablement éthyle, en particulier en le composé acide N-(3-carboxy-1-oxopropyl)-(4S)-p-phénylphénylméthyl)-4-amino-(2R)-méthylbutanoïque ou un sel correspondant, ou l'ester éthylique de l'acide N-(3-carboxy-1-oxopropyl)-(4S)-p-phénylphénylméthyl)-4-amino-(2R)-méthylbutanoïque ou un sel correspondant, en éliminant éventuellement - s'il est présent - un quelconque groupe protecteur d'azote Re,
    éventuellement la mise en réaction du composé obtenu de formule (VIII), Ra et Re étant hydrogène avec un réactif de couplage en présence d'un C1-C6-alkanol, préférablement d'éthanol, pour fournir le composé de formule (VIII), Ra étant choisi parmi C1-C6-alkyle, préférablement éthyle, et Re étant hydrogène, et
    la mise en réaction du composé de formule (VIII), Ra étant choisi parmi hydrogène et C1-C6-alkyle, préférablement éthyle, et Re étant hydrogène, avec l'anhydride de l'acide succinique.
  13. Utilisation d'un composé selon l'une quelconque des revendications 1 à 4 dans la préparation de l'acide N-(3-carboxy-1-oxopropyl)-(4S)-p-phénylphénylméthyl)-4-amino-(2R)-méthylbutanoïque ou d'un sel correspondant, ou de l'ester éthylique de l'acide N-(3-carboxy-1-oxopropyl)-(4S)-p-phénylphénylméthyl)-4-amino-(2R)-méthylbutanoïque ou d'un sel correspondant, ou de l'hemipentahydrate de [3-((1S,3R)-1-biphényl-4-ylméthyl-3-éthoxycarbonyl-1-butylcarbamoyl)propionate-(S)-3'-méthyl-2'-(pentanoyl{2''-(tétrazol-5-ylate)biphényl-4'-ylméthyl}amino)butyrate] trisodique.
EP16810077.4A 2015-12-10 2016-12-08 Intermédiaires pour la production de sacubitril et leurs preparation Active EP3386955B1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP15199378 2015-12-10
EP16198655 2016-11-14
PCT/IB2016/057435 WO2017098430A1 (fr) 2015-12-10 2016-12-08 Nouveau procédé et composés intermédiaires

Publications (2)

Publication Number Publication Date
EP3386955A1 EP3386955A1 (fr) 2018-10-17
EP3386955B1 true EP3386955B1 (fr) 2020-08-05

Family

ID=57543110

Family Applications (1)

Application Number Title Priority Date Filing Date
EP16810077.4A Active EP3386955B1 (fr) 2015-12-10 2016-12-08 Intermédiaires pour la production de sacubitril et leurs preparation

Country Status (14)

Country Link
US (2) US10377697B2 (fr)
EP (1) EP3386955B1 (fr)
JP (1) JP7001597B2 (fr)
KR (1) KR20180093984A (fr)
CN (1) CN108602785B (fr)
AU (1) AU2016366795B2 (fr)
CA (1) CA3006254A1 (fr)
ES (1) ES2830266T3 (fr)
HK (1) HK1254367A1 (fr)
IL (1) IL259454B (fr)
JO (1) JO3771B1 (fr)
RU (1) RU2769445C2 (fr)
TW (1) TWI628158B (fr)
WO (1) WO2017098430A1 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7001597B2 (ja) 2015-12-10 2022-01-19 ノバルティス アーゲー 新規なプロセスおよび中間体
WO2017148357A1 (fr) 2016-02-29 2017-09-08 广东东阳光药业有限公司 Intermédiaire de sacubitril et procédé de préparation correspondant
CN107602399B (zh) * 2016-07-11 2020-09-25 江西东邦药业有限公司 一种脑啡肽酶抑制剂中间体的制备方法
WO2018231462A1 (fr) * 2017-06-14 2018-12-20 Codexis, Inc. Polypeptides transaminase modifiés destinés à une biocatalyse industrielle
UY38072A (es) 2018-02-07 2019-10-01 Novartis Ag Compuestos derivados de éster butanoico sustituido con bisfenilo como inhibidores de nep, composiciones y combinaciones de los mismos
CN112930174A (zh) * 2018-10-31 2021-06-08 波利科尔疗法公司 用于合成d3多巴胺受体激动剂的方法
CN111748590B (zh) * 2019-03-29 2024-05-28 弈柯莱(台州)药业有限公司 转氨酶在制备Sacubitril中间体中的应用
CN110878039A (zh) * 2019-12-18 2020-03-13 株洲千金药业股份有限公司 一种沙库巴曲缬沙坦钠杂质的制备方法

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU644281B2 (en) * 1991-04-24 1993-12-02 Sumitomo Pharmaceuticals Company, Limited Novel thiazole derivatives
US5217996A (en) 1992-01-22 1993-06-08 Ciba-Geigy Corporation Biaryl substituted 4-amino-butyric acid amides
WO2003062225A1 (fr) 2002-01-23 2003-07-31 Bayer Pharmaceuticals Corporation Derives pyrimidine en tant qu'inhibiteurs de kinase rho
EP1903027A1 (fr) * 2006-09-13 2008-03-26 Novartis AG Procédé de préparation de l' acide amino-4 butyrique ou de ses derivés et leur utilisation pour la préparation des inhibiteurs NEP
WO2008036420A2 (fr) 2006-09-21 2008-03-27 Achillion Pharmaceuticals, Inc. 9-(hétéroaryl)-isothiazolo[5,4-b]quinoléine-3,4-diones et composés apparentés en tant qu'agents anti-infectieux
SG185318A1 (en) * 2007-01-12 2012-11-29 Novartis Ag Process for preparing 5-biphenyl-4-amino-2-methyl pentanoic acid
AR070176A1 (es) 2008-01-17 2010-03-17 Novartis Ag Procesos de sintesis de inhibidores de nep, compuestos intermediarios y uso de los mismos en la sintesis
CN101684077B (zh) * 2008-09-24 2013-01-02 浙江九洲药业股份有限公司 N-酰基联苯丙氨酸的制备方法
HUE052297T2 (hu) 2009-01-08 2021-04-28 Codexis Inc Transzamináz polipeptidek
CN101774941A (zh) 2009-01-13 2010-07-14 浙江九洲药业股份有限公司 2-酰基氨基-3-联苯基丙酸的制备及拆分方法
KR20120093227A (ko) 2009-09-23 2012-08-22 노파르티스 아게 Ｎ-아실비페닐 알라닌의 제조 방법
US9067883B2 (en) 2010-01-22 2015-06-30 Novartis Ag Intermediates of neutral endopeptidase inhibitors and preparation method thereof
AU2011295171B2 (en) 2010-08-23 2015-01-15 Novartis Ag New process for the preparation of intermediates useful for the manufacture NEP inhibitors
JP5705984B2 (ja) 2010-08-23 2015-04-22 ノバルティス アーゲー Ｎｅｐ阻害剤を製造するための中間体の調製方法
AR092278A1 (es) 2012-08-31 2015-04-08 Novartis Ag Proceso de obtencion de derivados n-acilicos de bifenil-alanina e intermediarios relacionados
CN104557600B (zh) 2015-01-26 2016-05-04 苏州明锐医药科技有限公司 沙库比曲的制备方法
WO2016135751A1 (fr) 2015-02-25 2016-09-01 Mylan Laboratories Limited Nouveau procédé pour la préparation de sacubitril et de ses intermédiaires
CN105330569A (zh) 2015-09-11 2016-02-17 天台宜生生化科技有限公司 一种(r)-2-(n-叔丁氧羰基氨基)联苯丙醇的制备方法
CN105152980B (zh) 2015-09-11 2017-03-29 浙江永宁药业股份有限公司 N‑叔丁氧羰基‑（4s）‑（对苯基苯基甲基）‑4‑氨基‑（2r）‑甲基丁酸的手性制备方法
JP7001597B2 (ja) 2015-12-10 2022-01-19 ノバルティス アーゲー 新規なプロセスおよび中間体
CN106977415B (zh) * 2016-01-15 2021-03-26 广东东阳光药业有限公司 一种沙库必曲的中间体及其制备方法
CN105601524B (zh) 2016-03-17 2017-09-29 海门慧聚药业有限公司 Lcz696关键中间体的制备
CN105884656B (zh) 2016-04-20 2017-09-19 沧州那瑞化学科技有限公司 一种lcz696中间体的制备方法
CN105753741A (zh) 2016-04-26 2016-07-13 常州制药厂有限公司 一种抗心衰药物Sacubitril中间体的制备方法
CN105924355B (zh) * 2016-05-11 2018-08-17 浙江宏元药业有限公司 沙库比曲中间体及沙库比曲中间体和沙库比曲的制备方法
CN107602399B (zh) * 2016-07-11 2020-09-25 江西东邦药业有限公司 一种脑啡肽酶抑制剂中间体的制备方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
None *

Also Published As

Publication number Publication date
CN108602785A (zh) 2018-09-28
CA3006254A1 (fr) 2017-06-15
TWI628158B (zh) 2018-07-01
JO3771B1 (ar) 2021-01-31
RU2018123321A (ru) 2020-01-10
RU2769445C2 (ru) 2022-03-31
CN108602785B (zh) 2023-08-11
WO2017098430A1 (fr) 2017-06-15
US20180354889A1 (en) 2018-12-13
US10377697B2 (en) 2019-08-13
AU2016366795A1 (en) 2018-06-07
US20190308927A1 (en) 2019-10-10
IL259454A (en) 2018-07-31
AU2016366795B2 (en) 2019-06-13
TW201725191A (zh) 2017-07-16
KR20180093984A (ko) 2018-08-22
IL259454B (en) 2021-01-31
JP7001597B2 (ja) 2022-01-19
US11434192B2 (en) 2022-09-06
EP3386955A1 (fr) 2018-10-17
ES2830266T3 (es) 2021-06-03
HK1254367A1 (zh) 2019-07-19
JP2018538295A (ja) 2018-12-27
RU2018123321A3 (fr) 2020-03-06

Similar Documents

Publication Publication Date Title
EP3386955B1 (fr) Intermédiaires pour la production de sacubitril et leurs preparation
EP3558930B1 (fr) Nouveau procédé pour des intermédiaires de sacubitril précoces
KR100947758B1 (ko) 벤즈옥사진 유도체의 제조를 위한 중간체
JP4142444B2 (ja) ２−アゼチジノン誘導体の製造方法
JP2016164143A (ja) Ｈｃｖの大環状プロテアーゼ阻害剤の製造のための方法及び中間体
KR20210002522A (ko) 4-시아노-치환된 1-아미노인단 및 오자니모드의 거울상이성질체 선택적 생체촉매적 제조
JP2000516586A (ja) ３―ピロリン―２―カルボン酸誘導体の製法
EP2038260B1 (fr) Synthèse stéréosélective de (s)-1-méthyl-3-phénylpipérazine
KR100870784B1 (ko) 효모로부터 유래한 콜레스테롤에스터라제에 의한엔안티오퓨어 중간체의 효소적 합성
JP5329973B2 (ja) リパーゼ触媒を用いるエナンチオ選択的アシル化とその後の硫酸による沈殿によって、ラセミ体の４−（１−アミノエチル）安息香酸メチルエステルから（ｒ）−および（ｓ）−４−（１−アンモニウムエチル）安息香酸メチルエステル硫酸塩を調製する方法
JPH0649005A (ja) ビニルグリシン（２−アミノ−３−ブテン酸）の簡単な製造方法および誘導体の簡便な分割
RU2258069C2 (ru) Способ получения производного бензоксазина, способы получения его промежуточного соединения и промежуточные соединения
JPWO2016208709A1 (ja) １−（アシルオキシ）アルキルカルバメート誘導体の新規な製造方法
MX2008008282A (en) Preparation of gamma-amino acids having affinity for the alpha-2-delta protein

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: UNKNOWN

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20180710

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

AX Request for extension of the european patent

Extension state: BA ME

DAV Request for validation of the european patent (deleted)
DAX Request for extension of the european patent (deleted)
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1254367

Country of ref document: HK

REG Reference to a national code

Ref country code: DE

Ref legal event code: R079

Ref document number: 602016041525

Country of ref document: DE

Free format text: PREVIOUS MAIN CLASS: C07D0267020000

Ipc: C07C0251660000

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: GRANT OF PATENT IS INTENDED

RIC1 Information provided on ipc code assigned before grant

Ipc: C07C 251/66 20060101AFI20200128BHEP

Ipc: C07C 313/06 20060101ALI20200128BHEP

Ipc: C07D 265/02 20060101ALI20200128BHEP

Ipc: C07C 69/738 20060101ALI20200128BHEP

Ipc: C07C 229/34 20060101ALI20200128BHEP

INTG Intention to grant announced

Effective date: 20200226

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE PATENT HAS BEEN GRANTED

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: CH

Ref legal event code: EP

REG Reference to a national code

Ref country code: AT

Ref legal event code: REF

Ref document number: 1298539

Country of ref document: AT

Kind code of ref document: T

Effective date: 20200815

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 602016041525

Country of ref document: DE

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: LT

Ref legal event code: MG4D

REG Reference to a national code

Ref country code: NL

Ref legal event code: MP

Effective date: 20200805

REG Reference to a national code

Ref country code: AT

Ref legal event code: MK05

Ref document number: 1298539

Country of ref document: AT

Kind code of ref document: T

Effective date: 20200805

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: BG

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20201105

Ref country code: AT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20200805

Ref country code: LT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20200805

Ref country code: HR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20200805

Ref country code: SE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20200805

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20201106

Ref country code: NO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20201105

Ref country code: FI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20200805

Ref country code: PT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20201207

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20201205

Ref country code: RS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20200805

Ref country code: LV

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20200805

Ref country code: NL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20200805

Ref country code: PL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20200805

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CZ

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20200805

Ref country code: DK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20200805

Ref country code: RO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20200805

Ref country code: SM

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20200805

Ref country code: EE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20200805

REG Reference to a national code

Ref country code: DE

Ref legal event code: R097

Ref document number: 602016041525

Country of ref document: DE

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: AL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20200805

REG Reference to a national code

Ref country code: ES

Ref legal event code: FG2A

Ref document number: 2830266

Country of ref document: ES

Kind code of ref document: T3

Effective date: 20210603

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20200805

26N No opposition filed

Effective date: 20210507

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20200805

Ref country code: MC

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20200805

REG Reference to a national code

Ref country code: BE

Ref legal event code: MM

Effective date: 20201231

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LU

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20201208

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: TR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20200805

Ref country code: MT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20200805

Ref country code: CY

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20200805

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20200805

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: BE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20201231

P01 Opt-out of the competence of the unified patent court (upc) registered

Effective date: 20230514

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GB

Payment date: 20231116

Year of fee payment: 8

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: IT

Payment date: 20231128

Year of fee payment: 8

Ref country code: IE

Payment date: 20231123

Year of fee payment: 8

Ref country code: FR

Payment date: 20231122

Year of fee payment: 8

Ref country code: DE

Payment date: 20231121

Year of fee payment: 8

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: ES

Payment date: 20240115

Year of fee payment: 8

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: CH

Payment date: 20240101

Year of fee payment: 8