EP2514515B1 - Procédé de mélange d'un premier et d'un deuxième composant - Google Patents

Procédé de mélange d'un premier et d'un deuxième composant Download PDF

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Publication number
EP2514515B1
EP2514515B1 EP12156188.0A EP12156188A EP2514515B1 EP 2514515 B1 EP2514515 B1 EP 2514515B1 EP 12156188 A EP12156188 A EP 12156188A EP 2514515 B1 EP2514515 B1 EP 2514515B1
Authority
EP
European Patent Office
Prior art keywords
drum
container
obstacle structure
chamber
component
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Not-in-force
Application number
EP12156188.0A
Other languages
German (de)
English (en)
Other versions
EP2514515A1 (fr
Inventor
Martina Daub
Juergen Steigert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Robert Bosch GmbH
Original Assignee
Robert Bosch GmbH
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Filing date
Publication date
Application filed by Robert Bosch GmbH filed Critical Robert Bosch GmbH
Publication of EP2514515A1 publication Critical patent/EP2514515A1/fr
Application granted granted Critical
Publication of EP2514515B1 publication Critical patent/EP2514515B1/fr
Not-in-force legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/45—Magnetic mixers; Mixers with magnetically driven stirrers
    • B01F33/453—Magnetic mixers; Mixers with magnetically driven stirrers using supported or suspended stirring elements
    • B01F33/4533—Magnetic mixers; Mixers with magnetically driven stirrers using supported or suspended stirring elements supporting the stirring element in one point
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F29/00—Mixers with rotating receptacles
    • B01F29/15—Use of centrifuges for mixing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F29/00—Mixers with rotating receptacles
    • B01F29/30—Mixing the contents of individual packages or containers, e.g. by rotating tins or bottles
    • B01F29/32—Containers specially adapted for coupling to rotating frames or the like; Coupling means therefor
    • B01F29/321—Containers specially adapted for coupling to rotating frames or the like; Coupling means therefor of test-tubes or the like
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/42—Mixers with shaking, oscillating, or vibrating mechanisms with pendulum stirrers, i.e. with stirrers suspended so as to oscillate about fixed points or axes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/713—Feed mechanisms comprising breaking packages or parts thereof, e.g. piercing or opening sealing elements between compartments or cartridges
    • B01F35/7137—Piercing, perforating or melting membranes or closures which seal the compartments
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/716—Feed mechanisms characterised by the relative arrangement of the containers for feeding or mixing the components
    • B01F35/7161—Feed mechanisms characterised by the relative arrangement of the containers for feeding or mixing the components the containers being connected coaxially before contacting the contents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/717—Feed mechanisms characterised by the means for feeding the components to the mixer
    • B01F35/71725—Feed mechanisms characterised by the means for feeding the components to the mixer using centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
    • B01L2400/0683—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers mechanically breaking a wall or membrane within a channel or chamber
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

Definitions

  • the publication DE 30 36 538 C2 describes a pivot cup centrifuge for centrifuging blood. Purpose of such centrifuges is the separation of substances in the Zentrifugiergutraum taking advantage of inertia: particles or liquids with higher density migrate due to the higher inertia to the outside. In doing so, they displace the lower density components, which thereby reach the center.
  • a diffuser for introducing materials into a given material using centrifugal force wherein the diffuser comprises a rotor relatively rotating to a stator.
  • the method defined in claim 1 has the advantage over conventional solutions that - instead of the known separating of, for example, two Liquids of different densities - effective mixing of the two liquids can be achieved.
  • the obstacle structure is moved under the influence of centrifugal force in the two liquids in the container, whereby they are mixed together.
  • the mixing chamber comprising the container, the obstacle structure and the connecting piece can be easily mounted, for example, in a cartridge, due to the fact that they form a coherent part.
  • Component in this case means a liquid, a gas or a particle.
  • first and second component only two different states of the same substance may be meant:
  • the first component may be formed as a clumped portion and the second component as a liquid portion of the same substance.
  • the connecting piece is formed integrally with the container and / or the obstacle structure.
  • in one piece is meant here that the connecting piece, the container and / or the obstacle structure are formed from one and the same material.
  • the connector, the container and / or the obstacle structure can be easily produced by injection molding.
  • the connecting piece is connected to a frame, which is connected to the container, in particular glued, is.
  • the obstacle structure can be easily connected to the container.
  • the frame may in particular be connected to an upper, peripheral edge of the container. Instead of the frame, another component could be used.
  • the connecting piece is flexible or formed with a hinge.
  • the mobility to move the obstacle structure in the first and second components in the container can be easily provided, at the same time the one-piece Design of connector, container and obstacle structure is maintained.
  • one-piece is meant in the present case that the corresponding parts, in particular the connecting piece, the container and the obstacle structure, form a coherent part.
  • the connecting piece is elastically formed to generate a restoring force which counteracts the centrifugal force. If the centrifugal force falls below a predetermined threshold value or falls away completely, the obstacle structure automatically moves out of the first and second components in the container and / or the obstacle structure by means of this embodiment moves in the direction of the pivot point through the first and second components.
  • the obstacle structure constantly moves out of the first and second components and back into them and / or in the first and second components back and forth so that the first and second components are mixed well.
  • the connecting piece is connected to an edge or bottom of the container. This is advantageous in terms of manufacturing technology.
  • a mandrel is provided on the obstacle structure which is adapted to pierce a membrane closing an opening in the bottom of the container under the action of the centrifugal force.
  • the obstacle structure is designed as a beam, a rake, a sieve or a lattice structure. These structures are all well suited to mixing the first and second components together.
  • the connecting piece is arranged asymmetrically with respect to the obstacle structure such that the obstacle structure ters the connecting piece under the action of the centrifugal force, in particular elastically.
  • the obstacle structure can be rotated about an additional axis.
  • the first axis of rotation results for example from the fact that the connecting piece is flexible or elastic and thus bends under the action of centrifugal force.
  • a further axis of rotation results from the fact that the connecting piece is arranged asymmetrically with respect to the obstacle structure, wherein the further axis of rotation results by means of the twisting of the connecting piece.
  • the cartridge further comprises: a first drum having a first chamber, an adjusting device which is adapted to rotate the first drum about the central axis thereof when the centrifugal force exceeds a predetermined threshold, thereby the first chamber to conductively connect with a second chamber, wherein the first and / or second chamber is formed as the mixing chamber.
  • the first and / or second component can thus be transferred between the first and second chambers, with appropriate selection of the rotational speed of a centrifuge with the cartridge.
  • the corresponding components in the first and / or second chamber can be effectively mixed.
  • conductive is meant liquid, gas and / or particle-conducting in the present case.
  • the adjusting device comprises a first bevel, which cooperates with a second bevel of the first drum in order to engage it from a first position in which it is positively engaged with a housing of the cartridge in the direction of rotation about the central axis second position along the central axis and to spend against the action of a return means in which the positive engagement is canceled and the first drum rotates about the central axis.
  • a simple mechanism is provided for adjusting the first drum about at least two defined positions in the rotational direction about the central axis.
  • the mixing chamber can thus be the first drum forward and / or downstream or also provided in the first drum itself.
  • the mixing chamber can preferably be optionally connected to different further chambers as required.
  • a second drum which has the second chamber
  • a third drum which has the third chamber.
  • the second drum may also have the second chamber and the third chamber, for example. The same applies to the third drum.
  • the cartridge 100 includes a housing 102 in the form of a tube.
  • the housing 102 may be formed as a 5 to 100 mL, especially 50 mL, centrifuge tube, 1.5 mL or 2 mL Eppendorf tube, or alternatively as a microtiter plate (e.g., 20 ⁇ L per well).
  • the longitudinal axis of the housing 102 is designated 104.
  • a first drum 108, a second drum 106 and a third drum 110 are accommodated in the housing 102.
  • the drums 106, 108, 110 are arranged one behind the other and with their respective central axes coaxial with the longitudinal axis 104.
  • the housing 102 is formed closed at its one end 112. Between the closed end 112 and the adjacent to this third drum 110, a return means, for example in the form of a spring 114 is arranged.
  • the spring 114 may be in the form of a coil spring or a polymer, in particular an elastomer.
  • the other end 116 of the housing 102 is closed by means of a closure 118.
  • the closure 118 may be removed to remove the drums 106, 108, 110 from the housing 102.
  • the housing 102 itself can be dismantled to remove the drums 106, 108, 110 or to reach the chambers, for example the chamber 136.
  • the spring 114 is disposed between the shutter 118 and the second drum 106, so that the spring 114 is stretched to generate a restoring force.
  • Other arrangements of the spring 114 are conceivable.
  • the first drum 108 connected downstream of the second drum 106 comprises a mixing chamber 124 in which the reagents from the chambers 120 are mixed with the sample from the chamber 122.
  • the first drum 108 includes, for example, another chamber 126 in which the mixture 128 flows from the mixing chamber 124 through a solid phase 130.
  • the solid phase 130 may be a gel column, a silica matrix, or a filter.
  • the third drum 110 which is in turn connected downstream of the first drum 108, comprises a chamber 132 for receiving a waste product 134 from the chamber 126. Furthermore, the third drum 110 comprises a further chamber 136 for receiving the desired end product 138.
  • the cartridge 100 has an outer geometry, so that it can be used in a receptacle of a centrifuge, in particular in a receptacle of a swing-bucket rotor or fixed-angle rotor of a centrifuge.
  • the cartridge 100 is moved by one in FIG. 1 schematically indicated pivot point 140 rotated at high speed.
  • the pivot point 140 lies on the longitudinal axis 104, so that a corresponding centrifugal force 142 along the longitudinal axis 104 acts on each component of the cartridge 100.
  • the mixing chamber 124 is first to be fluidly connected to the chamber 122 to receive the sample from the chamber 122. Thereafter, the mixing chamber 124 is to be connected to the chambers 120 to receive the reagents from these. Subsequently, the reagents and the sample in the mixing chamber 124 are speed-controlled mixed. Similarly, the processes in the chambers 126, 132 and 136 are to be speed controlled.
  • FIG. 2A-2G perspective view of various components of the cartridge 100 from FIG. 1 , Based on Figures 2A-2G in particular an adjusting device 300 (see Fig. 3A ), which enables the speed-dependent control of the above-mentioned processes.
  • the housing 102 on its inside projections 200.
  • the projections 200 are radially from the housing inner wall 202 toward the longitudinal axis 104.
  • the projections 200 form between them slots 204 which extend along the longitudinal axis 104.
  • the projections 200 are formed at their one end in each case with a slope 206.
  • the slopes 206 face away from the pivot point 140 during operation of the centrifuge with the cartridge 100.
  • FIG. 2B shows the end 112 of the housing 102, which is formed according to this embodiment as a removable cap.
  • the end 112 has at its inner periphery a plurality of grooves 208 which extend along the longitudinal axis 104.
  • FIG. 2C shows the second drum 106 with the chambers 120, 122.
  • the second drum 106 has on its outer wall 210 a plurality of projections 212 which extend from the outer wall 210 radially outwardly.
  • the projections 212 of the second drum 106 engage in the slots 204 of the housing 102.
  • rotation of the second drum 106 about the longitudinal axis 104 is blocked.
  • the second drum 106 is slidable along the longitudinal axis 104 in the slots 204.
  • the second drum 106 furthermore has on its outer wall 210, in particular on its end 214 facing the first drum 108, a crown-like contour 216 which comprises a large number of bevels 218, 220.
  • Two bevels 218, 220 each form a point of the crown-like contour 216.
  • the ramps 218, 220 also face away from the pivot point 140 during operation of the centrifuge with the cartridge 100.
  • FIG. 2D shows a view of the second drum 106 from Figure 2C from underneath.
  • the underside 222 of the second drum 106 assigned to the end 214 has a plurality of openings 224 in order to connect the chambers 120, 122 to the mixing chamber 124 of the first drum 108 in liquid, gas and / or particle form (hereinafter "conductive").
  • the openings 224 may also conductively connect the chambers 120, 122 to the chamber 126 of the first drum 108.
  • a respective conductive connection is determined by the position of a respective opening 224 with respect to the chambers 124, 126. This position is achieved by rotating the first drum 108 relative to the second drum 106, as will be explained in more detail later.
  • FIG. 2E shows a lancing device 226, which in FIG. 1 not shown.
  • Lancing device 226 includes a plate 228 having one or more spikes 230 disposed adjacent to an opening 232 in plate 228, respectively.
  • the mandrels 230 serve to control a respective opening 224 in the underside 222 of the second drum 106 in a controlled manner, whereupon in particular liquid flows from the corresponding chamber 120, 122 through the opening 232 into the chambers 124 or 126.
  • Figure 2F shows the first drum 108 with the chambers 124, 126.
  • the first drum 108 has a plurality of projections 240 on its outer wall 238.
  • the protrusions 240 are configured to engage the slots 204 (as well as the protrusions 212 of the second drum 106). As long as the projections 240 are engaged with the slots 240, rotation of the first drum 108 about the longitudinal axis 104 is disabled. However, the projections 240 along with the first drum 108 are movable along the longitudinal axis 104 in the slots 204.
  • the projections 240 have bevels 242, which point in the direction of the pivot point 140 during operation of the centrifuge with the cartridge 100 and are formed corresponding to the bevels 206 and 220.
  • FIG. 2G shows the third drum 110 with the chambers 132, 136.
  • the third drum 110 has projections 244 which project from the outer wall 246 of the third drum 110, respectively.
  • the projections 244 are adapted to engage the grooves 208 of the end 112 so that the third drum 110 is slidable in the longitudinal direction 104 in the grooves 208. However, rotation of the third drum 110 about the longitudinal axis 104 is thus locked.
  • FIG. 3A-3E show several operating conditions during operation of the cartridge 100 FIG. 1 , wherein an additional drum 302 is shown, but this is not relevant in the present case.
  • the Figures 4A-4E correspond respectively with the Figures 3A-3E and illustrate the movement of the ramps 206, 218, 220, 242 relative to each other.
  • FIG. 3B shows an operating state of the cartridge 100 which is more advanced than that in FIG FIG. 4B shown condition.
  • the housing 102 is shown partially transparent to reveal the interior.
  • the projections 200, the slots 204, the bevels 206, the projections 212, the bevels 218, 220, the projections 240 and the bevels 242 form in conjunction with the return spring 141, the above-mentioned adjusting device 300 for defined rotation of the first drum 108 relative to the second drum and third drum 110 about the longitudinal axis 104.
  • FIGS. 3A and 4A show a first position in which the projections 240 of the first drum 108 engage in the slots 204 and thus a rotation of the first drum 108 is locked about the longitudinal axis 104. If the rotational speed of the centrifuge is increased, then the second drum 106 pushes, by means of the bevels 220 of the contour 216, onto the bevels 242 of the first drum 108 against the action of the spring 114, compressing the spring 114. As a result, the first drum 108 moves in a direction away from the pivot point 140 as indicated by the corresponding arrows in FIGS FIGS. 4A and 4B indicated. This movement is continued until the protrusions 240 disengage from the protrusions 200.
  • the spring 114 presses the first drum 108 by means of the third drum 110 again in the direction of the pivot point 140.
  • the second drum 106 together with their bevels 220 also back in Direction of the pivot point 140 moves, whereby the bevels 242 of the first drum 108 come to rest against the bevels 206 of the housing 102 and slide along this while performing a further rotational movement of the first drum 108 in a third position, as in Figures 4D and 4E shown.
  • the projections 240 of the first drum 108 are again located in the slots 204 of the housing 102, so that further rotation of the first drum 108 about the longitudinal axis 104 is again locked.
  • the process described above may be repeated as many times as desired to rotate the first drum 108 in a defined manner relative to the second drum 106 and third drum 110.
  • FIG. 5A shows in a section of the mixing chamber 124 from FIG. 1 according to an embodiment of the present invention.
  • the mixing chamber 124 comprises a container 500 for receiving at least two components. These are preferably those components which are provided by means of the second drum 106.
  • the components may be designed as reagents or samples, in particular blood samples.
  • FIG. 5A shows the container 500 as it contains a mixture of two liquids 502, 504.
  • the liquids 502, 504 may have the same or a different density.
  • the volume of liquid that can be received in the container 500 is typically up to 3 mL.
  • the mixing chamber 124 further comprises an obstacle structure 506 which is adapted to move under the action of a centrifugal force (ie, when the rotational speed of the centrifuge exceeds a predetermined threshold) through the fluids 502, 504 to thereby mix them.
  • a centrifugal force ie, when the rotational speed of the centrifuge exceeds a predetermined threshold
  • a connecting piece 508 is provided, which connects the obstacle structure 506 with the container 500.
  • the obstacle structure 506 thus forms with the connector 508 and the container 500 a single part, which is easy to handle, in particular, easy to assemble.
  • the obstacle structure 506 is movably provided opposite the container 500 to perform its mixing function.
  • the obstacle structure 506 together with the connecting piece 508 and the container 500 can be made of one piece, that is to say be made of the same material. This can be achieved, for example, simply by injection molding the obstacle structure 506, the connector 508 and the container 500.
  • the connector 508 may be connected at its container end with a frame 510 (since it is another embodiment, the frame 510 is shown in phantom).
  • the frame 510 is in turn connected to the container 500, in particular an upper peripheral edge 512 of the container 500, in particular adhesively bonded.
  • the obstacle structure 506 with the connector 508 and the frame 510 may be made in one piece, in particular by injection molding.
  • the connecting piece 508 is elastic.
  • the purpose of this embodiment is to provide for an automatic reset of the obstacle structure 506 for a reduction in the rotational speed of the centrifuge. That is, the obstacle structure 506 pivots about the attachment point 522 of the connector 508 to the container 500 or frame 510 when the rotational speed exceeds a predetermined threshold (eg, a rotational speed corresponding to 1000 g of acceleration of the mixing chamber 124) from the fulcrum 140 under the action of centrifugal force away into or through the fluids 502, 504. If the speed is reduced again below the predetermined threshold, the obstacle structure 506 pivots back towards the pivot point 140 due to the elasticity of the connector 508.
  • the mixing chamber 124 is typically designed for a speed corresponding to 10,000 g of acceleration. "g" here means the gravitational acceleration.
  • the mixing is effected once by the movement of the obstacle structure 506.
  • the mixing chamber 124 when used with the above-described cartridge 100, the mixing chamber 124 itself moves along the longitudinal direction 104 away from the fulcrum 140, as described above. This results in a further mixing effect.
  • a rigid connecting piece may also be used, which is connected to the container 500 by means of a joint. A provision could then be provided by a separate spring.
  • the mixing chamber 124 may be designed so that further process steps and structures are integrated, e.g. Sedimentation structures or channel or siphon structures for transferring and switching the liquids 502, 504.
  • the container 500, the obstruction structure 506 and / or the connector 508 may be made of the same or different polymers.
  • the one or more polymers are, in particular, thermoplastics, elastomers or thermoplastic elastomers. Examples are cycloolefin polymer (COP), cycloolefin copolymer (COC), polycarbonates (PC), polyamides (PA), polyurethanes (PU), polypropylene (PP), polyethylene terephthalate (PET) or poly (methyl methacrylate) ( PMMA).
  • COP cycloolefin polymer
  • COC cycloolefin copolymer
  • PC polycarbonates
  • PA polyamides
  • PU polyurethanes
  • PP polypropylene
  • PET polyethylene terephthalate
  • PMMA poly (methyl methacrylate)
  • the obstacle structure 506 and the connector 508 may each have a cross section that varies in their respective longitudinal direction.
  • longitudinal direction refers in particular to a direction away from the container 500.
  • the obstacle structure 508 may have at its free-running end an additional mass, for example in the form of a thickened region or a metallization, in order to increase the inertia effect and thus the oscillation amplitude with variation of the rotational speed.
  • FIG. 5B shows a mixing chamber 124 according to a variation over the embodiment according to FIG. 5A .
  • the connector 508 is preferably attached to the upper edge 512 container side, is in the embodiment according to FIG. 5B the connector 508 fixed to the container side to a bottom 514 of the container 500.
  • the obstacle structure 506 may have a mandrel 516 at its free end.
  • an opening 518 is provided, which is closed by a membrane 520, to prevent leakage of the liquids 502, 504 from the container 500.
  • the mandrel 516 moves into the membrane 520 due to the pivoting of the obstacle structure 506 around the connection point 522 and thereby destroys it.
  • the liquids 502, 504 can flow out of the container 500.
  • the threshold value is preferably above the threshold for the mixing of the liquids 502, 504 by means of the obstacle structure 506. This ensures that the liquids 502, 504 are first mixed and thereafter the membrane 520 is pierced first.
  • the opening 518 may be seen with a downstream chamber, such as the chamber 126 of the first drum 108 FIG. 1 , or one of the chambers 132, 136 of the third drum 110.
  • FIG. 5A shows in a plan view FIG. 5A various embodiments of the obstacle structure 506, which are particularly suitable for mixing the liquids 502, 504.
  • the obstacle structure 506 is formed in the form of a bar.
  • FIG. 6B shows a further embodiment, wherein a plurality of the particular bar-shaped obstacle structures 506 are provided, which are each connected via a connecting piece 508 with the container 500.
  • the bars 506 according to the embodiments 6A and 6B have, for example, a width of 0.1-3 mm.
  • the distance between the beams 506 may also be between 0.1-3 mm, for example. From the bars 506, side bars may exit (not shown).
  • the obstacle structure 506 is formed in the form of a rake.
  • the obstacle structure 506 in the form of a screen in particular in the form of a plate 600 with a plurality of holes 602 formed.
  • the holes 602 may be circular or rectangular.
  • a diameter of a respective hole 602 may be between 0.1 and 3 mm.
  • the obstacle structure 506 is formed in the form of a lattice structure.

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
  • Centrifugal Separators (AREA)

Claims (8)

  1. Procédé de mélange d'un premier et d'un deuxième composant (502, 504) au moyen d'une chambre de mélange (124), dans lequel la chambre de mélange (124) comprend un récipient (500) destiné à contenir le premier et le deuxième composant (502, 504), une structure d'obstacle (506) construite de façon mobile par rapport au récipient (500) pour mélanger le premier composant (502) avec le deuxième composant (504) sous l'action d'une force centrifuge (142) et une pièce de liaison (508) reliée au récipient et à la structure d'obstacle, dans lequel la pièce de liaison (508) est formée d'une seule pièce avec le récipient (500) et/ou avec la structure d'obstacle (506), présentant les étapes suivantes:
    remplir le récipient (500) de la chambre de mélange (124) avec le premier et le deuxième composant (502, 504); et
    produire la force centrifuge (142), dans lequel on déplace la structure d'obstacle (506) dans le premier et le deuxième composant (502, 504) et on mélange le premier et le deuxième composant l'un avec l'autre.
  2. Procédé selon la revendication 1, dans lequel la pièce de liaison (508) est assemblée à un cadre (510), qui est assemblé, en particulier collé, au récipient (500).
  3. Procédé selon l'une quelconque des revendications précédentes, dans lequel la pièce de liaison (508) est réalisée sous forme flexible.
  4. Procédé selon l'une quelconque des revendications précédentes, dans lequel la pièce de liaison (508) est réalisée sous forme élastique, afin de produire une force de rappel, qui s'oppose à la force centrifuge (142).
  5. Procédé selon l'une quelconque des revendications précédentes, dans lequel la pièce de liaison (508) est reliée à un bord (510) ou au fond (514) du récipient (500).
  6. Procédé selon l'une quelconque des revendications précédentes, dans lequel il est prévu sur la structure d'obstacle (506) une épine (516), qui est conçue pour percer sous l'action de la force centrifuge (142) une membrane (520) fermant une ouverture (518) dans le fond (514) du récipient (500).
  7. Procédé selon l'une quelconque des revendications précédentes, dans lequel la structure d'obstacle (506) est réalisée sous la forme d'une poutre, d'un râteau, d'un tamis ou d'une structure de grille.
  8. Procédé selon l'une quelconque des revendications précédentes, dans lequel la pièce de liaison (508) est disposée de façon asymétrique par rapport à la structure d'obstacle (506), de telle manière que la structure d'obstacle (506) torde, en particulier de façon élastique, la pièce de liaison (508) sous l'action de la force centrifuge (142).
EP12156188.0A 2011-04-20 2012-02-20 Procédé de mélange d'un premier et d'un deuxième composant Not-in-force EP2514515B1 (fr)

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DE102011007779A DE102011007779A1 (de) 2011-04-20 2011-04-20 Mischkammer, Kartusche sowie Verfahren zum Mischen einer ersten und zweiten Komponente

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DE102011077134A1 (de) * 2011-06-07 2012-12-13 Robert Bosch Gmbh Kartusche, Zentrifuge sowie Verfahren zum Mischen einer ersten und zweiten Komponente
DE102011077115A1 (de) * 2011-06-07 2012-12-13 Robert Bosch Gmbh Kartusche, Zentrifuge sowie Verfahren
DE102011077124A1 (de) * 2011-06-07 2012-12-13 Robert Bosch Gmbh Kartusche, Zentrifuge sowie Verfahren
WO2013096621A2 (fr) * 2011-12-23 2013-06-27 The Broad Institute, Inc. Dispositif et procédé de fragmentation de polymères et de particules
CN116273222A (zh) * 2017-09-06 2023-06-23 万迈医疗仪器有限公司 用于搅动微孔中的液体的铁磁转子
CN109261040A (zh) * 2018-12-04 2019-01-25 希肯医疗技术（苏州）有限公司 一种多功能试剂锅
CN112915877B (zh) * 2021-02-05 2022-06-10 刘见萍 一种医用血试剂样品混合装置
CN115816683B (zh) * 2023-02-15 2023-06-20 云南田野橡胶集团有限公司 一种橡胶制备搅拌混合设备及橡胶固色混合搅拌方法

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CN102743999A (zh) 2012-10-24
US9555383B2 (en) 2017-01-31
DE102011007779A1 (de) 2012-10-25
EP2514515A1 (fr) 2012-10-24
US20120269030A1 (en) 2012-10-25

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