EA201990924A1 - Создание маиса, устойчивого к северной пятнистости листьев - Google Patents

Создание маиса, устойчивого к северной пятнистости листьев

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Publication number
EA201990924A1
EA201990924A1 EA201990924A EA201990924A EA201990924A1 EA 201990924 A1 EA201990924 A1 EA 201990924A1 EA 201990924 A EA201990924 A EA 201990924A EA 201990924 A EA201990924 A EA 201990924A EA 201990924 A1 EA201990924 A1 EA 201990924A1
Authority
EA
Eurasian Patent Office
Prior art keywords
nlb18
methods
leaves
modified
mais
Prior art date
Application number
EA201990924A
Other languages
English (en)
Inventor
Хойжун Гао
Байлинь Ли
Роберт Б. Мили
Леандро Даниэль Перуджини
Гирма М. Табор
Original Assignee
Пайонир Хай-Бред Интернэшнл, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Пайонир Хай-Бред Интернэшнл, Инк. filed Critical Пайонир Хай-Бред Интернэшнл, Инк.
Publication of EA201990924A1 publication Critical patent/EA201990924A1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

В данном документе предусматриваются композиции и способы получения растительных клеток с модифицированными нуклеотидными последовательностями Ht1, модифицированными последовательностями NLB18 или как теми, так и другими. Способы предусматривают введение в геном маиса двухнитевых разрывов в эндогенной последовательности, кодирующей Ht1, эндогенной последовательности, кодирующей NLB18, или как в той, так и в другой, для модификации геномной последовательности с целью повышения устойчивости к северной пятнистости листьев растения, полученного из растительной клетки. Дополнительно предусматриваются способы, посредством которых вводят связанные с устойчивостью аллели Ht1 и/или NLB18 в специфические сайты в геноме. Также охватываются растения, полученные из растительных клеток, и семена, полученные от растений. Также предусматриваются направляющие полинуклеотиды для применения системы CRISPR-Cas в индуцировании двухнитевых разрывов.
EA201990924A 2016-10-13 2017-10-10 Создание маиса, устойчивого к северной пятнистости листьев EA201990924A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662407867P 2016-10-13 2016-10-13
PCT/US2017/055835 WO2018071362A1 (en) 2016-10-13 2017-10-10 Generating northern leaf blight resistant maize

Publications (1)

Publication Number Publication Date
EA201990924A1 true EA201990924A1 (ru) 2019-09-30

Family

ID=60153565

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201990924A EA201990924A1 (ru) 2016-10-13 2017-10-10 Создание маиса, устойчивого к северной пятнистости листьев

Country Status (9)

Country Link
US (1) US12012605B2 (ru)
EP (2) EP3525578A1 (ru)
CN (1) CN109844121A (ru)
AR (1) AR109941A1 (ru)
BR (1) BR112019007327A2 (ru)
CA (1) CA3037560A1 (ru)
EA (1) EA201990924A1 (ru)
WO (1) WO2018071362A1 (ru)
ZA (1) ZA201901854B (ru)

Families Citing this family (9)

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WO2011163590A1 (en) 2010-06-25 2011-12-29 E. I. Du Pont De Nemours And Company Compositions and methods for enhancing resistance to northern leaf blight in maize
EA037614B1 (ru) 2015-10-16 2021-04-21 Пайонир Хай-Бред Интернэшнл, Инк. Получение растений маиса с повышенной устойчивостью к северной пятнистости листьев
WO2021257206A1 (en) * 2020-06-17 2021-12-23 Pioneer Hi-Bred International, Inc. Generating maize plants with enhanced resistance to northern leaf blight
EP3525578A1 (en) 2016-10-13 2019-08-21 Pioneer Hi-Bred International, Inc. Generating northern leaf blight resistant maize
EP3447134B1 (en) * 2017-08-22 2023-10-11 KWS SAAT SE & Co. KGaA Increased fungal resistance in crop plants
CA3105847A1 (en) * 2018-07-23 2020-01-30 Pioneer Hi-Bred International, Inc. Methods and compositions to increase yield through modifications of fea3 genomic locus and associated ligands
US20230059309A1 (en) * 2020-01-09 2023-02-23 Pioneer Hi-Bred International, Inc. Two-step gene swap
CA3186862A1 (en) * 2020-08-18 2022-02-24 Pioneer Hi-Bred International, Inc. Multiple disease resistance genes and genomic stacks thereof
WO2023164453A2 (en) * 2022-02-23 2023-08-31 Pioneer Hi-Bred International, Inc. Multiple disease resistance genes and genomic stacks thereof

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Also Published As

Publication number Publication date
US20220275392A1 (en) 2022-09-01
EP3525578A1 (en) 2019-08-21
US12012605B2 (en) 2024-06-18
CA3037560A1 (en) 2018-04-19
EP4242330A3 (en) 2023-11-22
BR112019007327A2 (pt) 2019-07-02
CN109844121A (zh) 2019-06-04
AR109941A1 (es) 2019-02-06
EP4242330A2 (en) 2023-09-13
WO2018071362A1 (en) 2018-04-19
ZA201901854B (en) 2019-11-27

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