EA201400156A1 - Способ определения активности протеолитического фермента (варианты), устройство для его реализации и способ диагностики - Google Patents

Способ определения активности протеолитического фермента (варианты), устройство для его реализации и способ диагностики

Info

Publication number
EA201400156A1
EA201400156A1 EA201400156A EA201400156A EA201400156A1 EA 201400156 A1 EA201400156 A1 EA 201400156A1 EA 201400156 A EA201400156 A EA 201400156A EA 201400156 A EA201400156 A EA 201400156A EA 201400156 A1 EA201400156 A1 EA 201400156A1
Authority
EA
Eurasian Patent Office
Prior art keywords
sample
proteolytic enzyme
activity
label
spatial
Prior art date
Application number
EA201400156A
Other languages
English (en)
Other versions
EA028341B1 (ru
Inventor
Фазоил Иноятович АТАУЛЛАХАНОВ
Наталья Михайловна ДАШКЕВИЧ
Михаил Владимирович ОВАНЕСОВ
Василий Иванович САРБАШ
Михаил Александрович ПАНТЕЛЕЕВ
Сергей Сергеевич КАРАМЗИН
Андрей Юрьевич КОНДРАТОВИЧ
Original Assignee
Общество с ограниченной ответственностью "ГемаКор Лабс" (ООО "ГемаКор Лабс")
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Общество с ограниченной ответственностью "ГемаКор Лабс" (ООО "ГемаКор Лабс") filed Critical Общество с ограниченной ответственностью "ГемаКор Лабс" (ООО "ГемаКор Лабс")
Publication of EA201400156A1 publication Critical patent/EA201400156A1/ru
Publication of EA028341B1 publication Critical patent/EA028341B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/974—Thrombin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Neurosurgery (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Изобретение относится к медицине и биологии и может быть использовано, в частности, для диагностических и исследовательских целей для определения характеристик системы свертывания крови и ее компонентов. Задачей настоящего изобретения является исследование пространственной динамики факторов свертывания в процессе роста фибринового сгустка и оценка роли факторов свертывания в различных фазах процесса свертывания крови в гетерогенной системе, что позволяет диагностировать отдельные заболевания и оценивать активность препаратов, влияющих на параметры свертывания крови. Поставленная задача решена путем создания способа определения пространственно-временного распределения активности протеолитического фермента в гетерогенной системе, в котором обеспечивают систему in vitro, которая содержит образец исследуемой среды, выбранной из группы, состоящей из плазмы крови, цельной крови, воды, лимфы, коллоидного раствора, кристаллоидного раствора или геля, и распределенный в образце протеолитический фермент или его предшественник, добавляют в систему in vitro флуорогенный субстрат с последующим высвобождением метки при расщеплении указанного субстрата протеолитическим ферментом, освещают образец исследуемой среды в заданные моменты времени для возбуждения сигнала флуоресценции метки, регистрируют одновременно с освещением в заданные моменты времени пространственное распределение сигнала флуоресценции метки в образце, получают из совокупности пространственных распределений сигнала флуоресцентной метки для заданных моментов времени пространственно-временное распределение активности протеолитического
EA201400156A 2011-07-26 2012-07-16 Способ определения активности протеолитического фермента (варианты), устройство для его реализации и способ диагностики EA028341B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
RU2011131293/10A RU2518247C2 (ru) 2011-07-26 2011-07-26 Способ определения пространственно-временного распределения активности протеолитического фермента в гетерогенной системе, устройство для реализации указанного способа и способ диагностики нарушений системы гемостаза по изменению пространственно-временного распределения активности протеолитического фермента в гетерогенной системе
PCT/RU2012/000570 WO2013015717A2 (ru) 2011-07-26 2012-07-16 Способ - определения пространственно-временного распределения активности протеолитического фермента в гетерогенной системе (варианты), устройство для реализации указанного способа и способ диагностики нарушений системы гемостаза по изменению пространственно-временного распределения активности протеолитического фермента в гетерогенной системе

Publications (2)

Publication Number Publication Date
EA201400156A1 true EA201400156A1 (ru) 2014-08-29
EA028341B1 EA028341B1 (ru) 2017-11-30

Family

ID=47601696

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201400156A EA028341B1 (ru) 2011-07-26 2012-07-16 Способ определения активности протеолитического фермента (варианты), устройство для его реализации и способ диагностики

Country Status (8)

Country Link
US (1) US9938563B2 (ru)
EP (1) EP2752495B8 (ru)
JP (1) JP2014521952A (ru)
CN (1) CN103998620A (ru)
CA (1) CA2842594C (ru)
EA (1) EA028341B1 (ru)
RU (1) RU2518247C2 (ru)
WO (1) WO2013015717A2 (ru)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105738378A (zh) * 2016-02-23 2016-07-06 上海航天动力科技工程有限公司 一种多功能自显像着色渗透剂及其应用
ES2857686T3 (es) * 2016-11-30 2021-09-29 Obschestvo S Ogranichennoy Otvetstvennostyu Hemacore Labs Dispositivo para monitorizar la dinámica espacial y temporal de trombina
RU2657294C1 (ru) * 2016-12-15 2018-06-13 Государственное бюджетное учреждение здравоохранения Московской области "Московский областной научно-исследовательский клинический институт им. М.Ф. Владимирского" (ГБУЗ МО МОНИКИ им. М.Ф. Владимирского) Устройство для количественной оценки флюоресценции и оптических свойств тканей in vivo
JP7273631B2 (ja) * 2019-06-26 2023-05-15 株式会社日立製作所 検体性状識別装置、検体性状識別方法及び検体搬送システム
US20240044921A1 (en) * 2020-12-28 2024-02-08 Fujimori Kogyo Co., Ltd. Method for evaluating blood coagulation performance, and method for inspecting risk of thrombosis
CN114465969B (zh) * 2021-12-23 2023-07-04 珠海格力电器股份有限公司 通讯消息组的管理方法、装置、设备和存储介质

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3516579A1 (de) * 1984-11-19 1986-05-22 Boehringer Mannheim Gmbh, 6800 Mannheim Gerinnungstest auf teststreifen
EP0608235B2 (en) * 1991-11-13 2005-05-04 T.A.C. Thrombosis and Coagulation Aktiebolag Method for the diagnosis of blood coagulation disorders
US5339830A (en) 1992-01-21 1994-08-23 Blake Joseph W Iii Blood coagulation test system
DK1159448T3 (da) * 1999-03-04 2004-08-09 Synapse Bv Bestemmelse af biologisk aktive former af proteolytiske enzymer
WO2003023360A2 (en) * 2001-09-10 2003-03-20 Meso Scale Technologies, Llc Methods and apparatus for conducting multiple measurements on a sample
CA2484554C (en) * 2002-05-01 2012-12-04 Synapse B.V. Diagnostic test for determining the concentration of transient proteolytic activity in composite biological media
EP1717588A1 (en) * 2005-04-29 2006-11-02 Synapse B.V. Measuring thrombin activity in whole blood
US8138308B2 (en) * 2005-12-27 2012-03-20 Mcmaster University Modified peptide substrate
US8133696B2 (en) * 2006-06-06 2012-03-13 Thrombinoscope B.V. Method for determining thrombin activity
JP5145426B2 (ja) * 2007-11-02 2013-02-20 メドプラスト ソシエテ アノニム 空間的な線維素凝塊形成を監視するための方法及び装置
EP2088435B1 (en) * 2008-02-07 2011-07-13 Synapse B.V. Time-course measurement of enzymatic activity corrected for impacts of disturbances relating to the reaction of the enzyme with a substrate
RU2395812C2 (ru) 2008-11-14 2010-07-27 Общество с ограниченной ответственностью "Медицинские инновации" Устройство для исследования пространственного свертывания крови и ее компонентов

Also Published As

Publication number Publication date
EP2752495A2 (en) 2014-07-09
US9938563B2 (en) 2018-04-10
JP2014521952A (ja) 2014-08-28
RU2011131293A (ru) 2013-02-10
WO2013015717A8 (ru) 2014-02-20
CN103998620A (zh) 2014-08-20
EP2752495B1 (en) 2020-03-25
EA028341B1 (ru) 2017-11-30
CA2842594C (en) 2020-01-07
WO2013015717A3 (ru) 2013-04-11
RU2518247C2 (ru) 2014-06-10
WO2013015717A2 (ru) 2013-01-31
CA2842594A1 (en) 2013-01-31
US20140227726A1 (en) 2014-08-14
EP2752495A4 (en) 2015-04-15
EP2752495B8 (en) 2020-04-29

Similar Documents

Publication Publication Date Title
EA201400156A1 (ru) Способ определения активности протеолитического фермента (варианты), устройство для его реализации и способ диагностики
Li et al. The strength of mechanical forces determines the differentiation of alveolar epithelial cells
Espuny-Camacho et al. Pyramidal neurons derived from human pluripotent stem cells integrate efficiently into mouse brain circuits in vivo
Gray et al. Identification of therapeutic covariant microRNA clusters in hypoxia-treated cardiac progenitor cell exosomes using systems biology
Navarrete et al. Screening drug-induced arrhythmia using human induced pluripotent stem cell–derived cardiomyocytes and low-impedance microelectrode arrays
Cho et al. Dual modulation of the mitochondrial permeability transition pore and redox signaling synergistically promotes cardiomyocyte differentiation from pluripotent stem cells
Reitinger et al. Electric impedance sensing in cell-substrates for rapid and selective multipotential differentiation capacity monitoring of human mesenchymal stem cells
Zhu et al. Two dimensional electrophysiological characterization of human pluripotent stem cell-derived cardiomyocyte system
Piccinato et al. High OCT4 and low p16INK4A expressions determine in vitro lifespan of mesenchymal stem cells
Ivanyuk et al. Ascorbic acid-induced cardiac differentiation of murine pluripotent stem cells: transcriptional profiling and effect of a small molecule synergist of Wnt/β-catenin signaling pathway
Chen et al. Dissipation monitoring for assessing EGF-induced changes of cell adhesion
RU2013118341A (ru) Получение сыворотки
Charwat et al. Monitoring dynamic interactions of tumor cells with tissue and immune cells in a lab-on-a-chip
RU2011127117A (ru) Способы и применения, включающие гемсвязывающий белок 1
Yamasaki et al. Assessment of human bioengineered cardiac tissue function in hypoxic and re-oxygenized environments to understand functional recovery in heart failure
Deng et al. Scalable generation of sensory neurons from human pluripotent stem cells
Kraft et al. Astrocytic calcium waves signal brain injury to neural stem and progenitor cells
Bergström et al. Developmentally regulated collagen/integrin interactions confer adhesive properties to early postnatal neural stem cells
Jeong et al. Podoplanin is responsible for the distinct blood and lymphatic capillaries
Bardot et al. Notch signaling commits mesoderm to the cardiac lineage
EP3116989A1 (en) A novel, high-throughput, nanotopographic platform for screening cell migratory behavior
ES2886472T3 (es) Métodos para la determinación de la etapa de desarrollo de células del cúmulo humanas
Matsuo et al. Possible effects of inhibition of IKr and IKs on field-potential waveforms in the human iPS cell-derived cardiomyocytes sheet
Emechebe et al. Phosphoproteomic response of cardiac endothelial cells to ischemia and ultrasound
WO2013188437A3 (en) Ex vivo methods to identify circulating drug metabolites with drug interaction potential

Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ KG TJ TM

QB4A Registration of a licence in a contracting state