EA201300780A1 - Способ получения индуцированных плюрипотентных стволовых клеток и дифференцированных клеток - Google Patents

Способ получения индуцированных плюрипотентных стволовых клеток и дифференцированных клеток

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Publication number
EA201300780A1
EA201300780A1 EA201300780A EA201300780A EA201300780A1 EA 201300780 A1 EA201300780 A1 EA 201300780A1 EA 201300780 A EA201300780 A EA 201300780A EA 201300780 A EA201300780 A EA 201300780A EA 201300780 A1 EA201300780 A1 EA 201300780A1
Authority
EA
Eurasian Patent Office
Prior art keywords
cells
pluripotent stem
induced pluripotent
differentiated
stem cells
Prior art date
Application number
EA201300780A
Other languages
English (en)
Other versions
EA028902B1 (ru
Inventor
Мигель Эстебан
Йоханнес Гриллари
Регина Гриллари
Дуаньцин Пэй
Тин Чжоу
Original Assignee
Университет Фюр Боденкультур Вена
Гуанчжоу Институт Ов Байомедисин Энд Хелт
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP11152519A external-priority patent/EP2481795A1/en
Application filed by Университет Фюр Боденкультур Вена, Гуанчжоу Институт Ов Байомедисин Энд Хелт filed Critical Университет Фюр Боденкультур Вена
Publication of EA201300780A1 publication Critical patent/EA201300780A1/ru
Publication of EA028902B1 publication Critical patent/EA028902B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Hematology (AREA)
  • Transplantation (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Представлены способы получения iPSCs и представляющих интерес дифференцированных клеток путем перепрограммирования донорских клеток, полученных неинвазивным способом. В частности, донорские клетки представляют собой отслоившиеся эпителиальные клетки из мочи (мочевые клетки). Дифференцированные клетки могут быть получены путем дифференцировки перепрограммированных iPSCs или путем прямого перепрограммирования мочевых клеток.
EA201300780A 2010-12-31 2011-12-23 Способ получения индуцированных плюрипотентных стволовых клеток и дифференцированных клеток EA028902B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CNPCT/CN2010/002226 2010-12-31
EP11152519A EP2481795A1 (en) 2011-01-28 2011-01-28 Method of generating induced pluripotent stem cells and differentiated cells
PCT/EP2011/073962 WO2012089669A1 (en) 2010-12-31 2011-12-23 Method of generating induced pluripotent stem cells and differentiated cells

Publications (2)

Publication Number Publication Date
EA201300780A1 true EA201300780A1 (ru) 2013-11-29
EA028902B1 EA028902B1 (ru) 2018-01-31

Family

ID=45464553

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201300780A EA028902B1 (ru) 2010-12-31 2011-12-23 Способ получения индуцированных плюрипотентных стволовых клеток и дифференцированных клеток

Country Status (10)

Country Link
US (1) US9926532B2 (ru)
EP (1) EP2658965B1 (ru)
JP (2) JP2014501114A (ru)
BR (1) BR112013016592B1 (ru)
CA (1) CA2823265C (ru)
DK (1) DK2658965T3 (ru)
EA (1) EA028902B1 (ru)
ES (1) ES2575605T3 (ru)
PT (1) PT2658965E (ru)
WO (1) WO2012089669A1 (ru)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9719068B2 (en) 2010-05-06 2017-08-01 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
JP2016507231A (ja) * 2013-02-05 2016-03-10 グアンジョウ インスティテュート オブ バイオメディスン アンド ヘルス、チャイニーズ アカデミー オブ サイエンスィズＧｕａｎｇｚｈｏｕ Ｉｎｓｔｉｔｕｔｅｓ Ｏｆ Ｂｉｏｍｅｄｉｃｉｎｅ Ａｎｄ Ｈｅａｌｔｈ， Ｃｈｉｎｅｓｅ Ａｃａｄｅｍｙ Ｏｆ Ｓｃｉｅｎｃｅｓ 幹細胞を使用する歯様構造物の調製
US11066649B2 (en) * 2014-03-19 2021-07-20 Institut National De La Sante Et De La Recherche Medicale (Inserm) Method for inducing human cholangiocyte differentiation
CA2949834A1 (en) 2014-05-28 2015-12-03 James Macormack Wells Methods and systems for converting precursor cells into gastric tissues through directed differentiation
JP6804438B2 (ja) 2014-10-17 2020-12-23 チルドレンズ ホスピタル メディカル センター 多能性幹細胞を使用するヒト小腸のｉｎ ｖｉｖｏモデル、並びにそれを作製、及び使用する方法
WO2016101010A1 (en) * 2014-12-22 2016-06-30 University Of South Australia Method of inducing beta cells from urine-derived cells using small molecules
CA2995582A1 (en) 2015-08-19 2017-02-23 Children's Research Institute, Children's National Medical Center Compositions and methods for treating graft versus host disease
CA3016641A1 (en) 2016-05-05 2017-11-09 Children's Hospital Medical Center Methods for the in vitro manufacture of gastric fundus tissue and compositions related to same
CN110062764A (zh) 2016-12-05 2019-07-26 儿童医院医学中心 结肠类器官及其制备和使用方法
KR102150489B1 (ko) * 2019-04-09 2020-09-01 고려대학교 산학협력단 소변세포로부터 신장전구세포로의 직접 역분화를 유도하는 방법 및 이의 방법으로 역분화된 신장전구세포를 포함하는 신장세포 손상 질환 예방 또는 치료용 약학 조성물
CN112143706A (zh) * 2020-09-27 2020-12-29 中国科学院广州生物医药与健康研究院 一种将尿液细胞重编程为诱导性多能干细胞的方法
CN113122505A (zh) * 2021-04-13 2021-07-16 四川大学华西医院 一种通过逆病毒获取尿源诱导多能干细胞的方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW438973B (en) 1995-12-19 2001-06-07 Sysmex Corp Apparatus and method for analyzing solid components in urine
JP3589113B2 (ja) * 1998-09-24 2004-11-17 田辺製薬株式会社 腎疾患治療薬およびそのスクリーニング方法
US20100111914A1 (en) 2007-05-21 2010-05-06 Yuanyuan Zhang Stem cells from urine and methods for using the same
KR101722033B1 (ko) * 2007-05-21 2017-03-31 웨이크 포리스트 유니버시티 헬스 사이언시즈 소변으로부터의 전구 세포 및 이를 사용하는 방법
EP3078738B1 (en) 2007-08-31 2020-05-20 Whitehead Institute for Biomedical Research Wnt pathway stimulation in reprogramming somatic cells
JP2011525794A (ja) * 2008-06-26 2011-09-29 国立大学法人大阪大学 ｉＰＳ細胞の製造方法および製造キット
CA2695590C (en) * 2008-06-27 2018-01-09 Kyoto University Method of efficiently establishing induced pluripotent stem cells
WO2010036923A1 (en) * 2008-09-25 2010-04-01 Salk Institute For Biological Studies Induced pluripotent stem cells and methods of use
US9249412B2 (en) * 2009-01-26 2016-02-02 Fred Zacouto Simplified method for partial genetic and epigenetic reprogramming of cells using siRNA specific for a heterochromatin protein 1
JP2010207162A (ja) * 2009-03-11 2010-09-24 Japan Health Science Foundation Ｂａｃクローンを用いる尿路上皮癌の発生リスク評価方法及び予後予測方法

Also Published As

Publication number Publication date
DK2658965T3 (da) 2016-06-13
US20130323782A1 (en) 2013-12-05
BR112013016592B1 (pt) 2022-03-15
US9926532B2 (en) 2018-03-27
JP2017169574A (ja) 2017-09-28
CA2823265A1 (en) 2012-07-05
WO2012089669A1 (en) 2012-07-05
BR112013016592A2 (pt) 2021-03-30
EP2658965B1 (en) 2016-03-02
EP2658965A1 (en) 2013-11-06
CA2823265C (en) 2019-05-21
JP2014501114A (ja) 2014-01-20
ES2575605T3 (es) 2016-06-29
PT2658965E (pt) 2016-06-06
EA028902B1 (ru) 2018-01-31

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Designated state(s): AM AZ BY KZ KG MD TJ TM