EA201101306A1 - Способ повышения выхода полипептидов - Google Patents

Способ повышения выхода полипептидов

Info

Publication number
EA201101306A1
EA201101306A1 EA201101306A EA201101306A EA201101306A1 EA 201101306 A1 EA201101306 A1 EA 201101306A1 EA 201101306 A EA201101306 A EA 201101306A EA 201101306 A EA201101306 A EA 201101306A EA 201101306 A1 EA201101306 A1 EA 201101306A1
Authority
EA
Eurasian Patent Office
Prior art keywords
increasing
polypeptides
output
approaching
optimal value
Prior art date
Application number
EA201101306A
Other languages
English (en)
Other versions
EA021205B1 (ru
Inventor
ВАН ДЕР Ян Метске ЛААН
Лиан Ву
Йоханнес Андрис Раубос
Люси Пареникова
Алрик Питер Лос
Ноэль Николас Мария Элизабет Пейдж Ван
Херман Ян Пел
Original Assignee
ДСМ АйПи АССЕТС Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ДСМ АйПи АССЕТС Б.В. filed Critical ДСМ АйПи АССЕТС Б.В.
Publication of EA201101306A1 publication Critical patent/EA201101306A1/ru
Publication of EA021205B1 publication Critical patent/EA021205B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Настоящее изобретение относится к способу повышения выхода белка. Способ включает в себя модифицирование набора релевантных характеристик белка до приведения их в оптимальный диапазон или приближения к оптимальному значению для одной или более характеристик в эукариотическом хозяине.
EA201101306A 2009-03-10 2010-03-08 Способ повышения выхода полипептидов EA021205B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP09154783 2009-03-10
PCT/EP2010/052918 WO2010102982A1 (en) 2009-03-10 2010-03-08 Method for improving the yield of a polypeptide

Publications (2)

Publication Number Publication Date
EA201101306A1 true EA201101306A1 (ru) 2012-02-28
EA021205B1 EA021205B1 (ru) 2015-04-30

Family

ID=40912127

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201101306A EA021205B1 (ru) 2009-03-10 2010-03-08 Способ повышения выхода полипептидов

Country Status (11)

Country Link
US (1) US9181573B2 (ru)
EP (1) EP2406386B1 (ru)
JP (1) JP2012520064A (ru)
CN (1) CN102741421B (ru)
AU (1) AU2010223354A1 (ru)
BR (1) BRPI1009189A2 (ru)
CA (1) CA2754851A1 (ru)
DK (1) DK2406386T3 (ru)
EA (1) EA021205B1 (ru)
MX (1) MX346700B (ru)
WO (1) WO2010102982A1 (ru)

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CN105308171B (zh) 2012-07-19 2019-03-08 帝斯曼知识产权资产管理有限公司 Agse缺陷菌株
US9951339B2 (en) 2012-09-19 2018-04-24 Dsm Ip Assets B.V. Cell modification method using essential genes as markers and optionally recycling these
WO2014202623A2 (en) * 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
MX370305B (es) 2013-12-02 2019-12-09 Dsm Ip Assets Bv Proteína estructurante de hielo.
WO2015091455A1 (en) 2013-12-17 2015-06-25 Dpx Holdings B.V. Bioreactor
KR102105352B1 (ko) * 2014-11-03 2020-04-29 메르크 파텐트 게엠베하 생체분자를 정제하기 위한 가용성 인테인 융합 단백질 및 방법
US11396665B2 (en) 2015-01-06 2022-07-26 Dsm Ip Assets B.V. CRISPR-CAS system for a filamentous fungal host cell
WO2016110511A1 (en) 2015-01-06 2016-07-14 Dsm Ip Assets B.V. A crispr-cas system for a lipolytic yeast host cell
US20180103650A1 (en) 2015-04-10 2018-04-19 Dsm Ip Assets B.V. Method for preparing a dough
US20180160695A1 (en) * 2015-06-02 2018-06-14 Dsm Ip Assets B.V. Use of ice structuring protein afp19 expressed in filamentous fungal strains for preparing food
CN109689856A (zh) 2016-07-13 2019-04-26 帝斯曼知识产权资产管理有限公司 用于海藻宿主细胞的CRISPR-Cas***
CN109715804A (zh) 2016-09-23 2019-05-03 帝斯曼知识产权资产管理有限公司 用于宿主细胞的指导rna表达***
WO2018166943A1 (en) 2017-03-13 2018-09-20 Dsm Ip Assets B.V. Zinc binuclear cluster transcriptional regulator-deficient strain
US20200032252A1 (en) 2017-04-06 2020-01-30 Dsm Ip Assets B.V. Self-guiding integration construct (sgic)
WO2019063849A1 (en) 2017-12-20 2019-04-04 Dsm Ip Assets B.V. PURIFICATION OF A POLYPEPTIDE OF INTEREST
EP3728603A2 (en) 2017-12-20 2020-10-28 DSM IP Assets B.V. A method for genome editing in a host cell
EP3790969A1 (en) 2018-05-09 2021-03-17 DSM IP Assets B.V. Crispr transient expression construct (ctec)
EP3891281A1 (en) 2018-12-05 2021-10-13 DSM IP Assets B.V. Crispr guide-rna expression strategies for multiplex genome engineering
DK3715464T3 (da) 2019-03-28 2021-07-26 Dsm Ip Assets Bv Drivhusgasforbedret fermentering
WO2021228880A1 (en) 2020-05-14 2021-11-18 Dsm Ip Assets B.V. Fed-batch fermentation process
CN112480139B (zh) * 2021-01-05 2022-04-19 遵义医科大学 罗丹明类荧光探针的制备方法及其检测有机自由基方法
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

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US5888773A (en) * 1994-08-17 1999-03-30 The United States Of America As Represented By The Department Of Health And Human Services Method of producing single-chain Fv molecules
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Also Published As

Publication number Publication date
BRPI1009189A2 (pt) 2015-09-15
AU2010223354A1 (en) 2011-09-08
EP2406386A1 (en) 2012-01-18
CN102741421A (zh) 2012-10-17
WO2010102982A1 (en) 2010-09-16
US9181573B2 (en) 2015-11-10
CN102741421B (zh) 2015-03-25
CA2754851A1 (en) 2010-09-16
MX346700B (es) 2017-03-28
EP2406386B1 (en) 2019-01-23
DK2406386T3 (en) 2019-04-23
MX2011009059A (es) 2011-11-18
US20110318752A1 (en) 2011-12-29
JP2012520064A (ja) 2012-09-06
EA021205B1 (ru) 2015-04-30

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Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM

MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): RU