EA200501101A1 - Способ получения рекомбинантных поликлональных белков - Google Patents

Способ получения рекомбинантных поликлональных белков

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Publication number
EA200501101A1
EA200501101A1 EA200501101A EA200501101A EA200501101A1 EA 200501101 A1 EA200501101 A1 EA 200501101A1 EA 200501101 A EA200501101 A EA 200501101A EA 200501101 A EA200501101 A EA 200501101A EA 200501101 A1 EA200501101 A1 EA 200501101A1
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Eurasian Patent Office
Prior art keywords
cells
collection
library
nucleic acid
recombinant polyclonal
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EA200501101A
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English (en)
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EA013225B1 (ru
Inventor
Джон С. Хаурум
Финн К. Виберг
Винсент В. Колье
Жаклин Шарон
Чиоу-Ин Ян
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Симфоген А/С
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Publication of EA200501101A1 publication Critical patent/EA200501101A1/ru
Publication of EA013225B1 publication Critical patent/EA013225B1/ru

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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    • C07—ORGANIC CHEMISTRY
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    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2833—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against MHC-molecules, e.g. HLA-molecules
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    • C07—ORGANIC CHEMISTRY
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    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/36—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against blood coagulation factors
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    • C07—ORGANIC CHEMISTRY
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    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
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    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
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    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

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Abstract

Настоящее изобретение относится к способу производства композиции рекомбинантного поликлонального белка, в особенности композиции рекомбинантного поликлонального антитела. Способ предусматривает получение коллекции клеток, трансфицированных библиотекой вариантных последовательностей нуклеиновой кислоты, в котором каждая клетка в коллекции трансфицирована и способна к экспрессии одного элемента библиотеки, который кодирует определенный элемент поликлонального белка, который связывает специфический антиген и который расположен на том же отдельном сайте в геноме отдельных клеток в указанной коллекции, в котором указанная последовательность нуклеиновой кислоты в природе не связана с указанной клеткой в коллекции. Клетки культивируют при подходящих условиях для экспрессии поликлонального белка, который получают из супернатанта культуры или клеток. Последовательность нуклеиновой кислоты вводят в клетки посредством трансфекции библиотекой векторов для сайт-специфичной интеграции. Настоящий способ подходит для производства рекомбинантных поликлональных антител, что, таким образом, делает возможной описанную выше замену полученных из плазмы терапевтических продуктов иммуноглобулина.Отчет о международном поиске был опубликован 2004.11.04.
EA200501101A 2003-01-07 2004-01-07 Способ получения линии клеток, продуцирующей рекомбинантный поликлональный белок, способ получения поликлонального белка, линия клеток, продуцирующая рекомбинантный поликлональный белок, библиотека векторов, популяция клеток EA013225B1 (ru)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US43840303P 2003-01-07 2003-01-07
DKPA200300008 2003-01-07
US47601803P 2003-06-05 2003-06-05
PCT/DK2004/000001 WO2004061104A2 (en) 2003-01-07 2004-01-07 Method for manufacturing recombinant polyclonal proteins

Publications (2)

Publication Number Publication Date
EA200501101A1 true EA200501101A1 (ru) 2006-02-24
EA013225B1 EA013225B1 (ru) 2010-04-30

Family

ID=32718469

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200501101A EA013225B1 (ru) 2003-01-07 2004-01-07 Способ получения линии клеток, продуцирующей рекомбинантный поликлональный белок, способ получения поликлонального белка, линия клеток, продуцирующая рекомбинантный поликлональный белок, библиотека векторов, популяция клеток

Country Status (19)

Country Link
US (1) US9163232B2 (ru)
EP (2) EP1583830B1 (ru)
JP (2) JP4589914B2 (ru)
KR (1) KR101024443B1 (ru)
AT (1) ATE338816T1 (ru)
AU (1) AU2004203727C1 (ru)
BR (1) BRPI0406678A (ru)
CA (1) CA2512647C (ru)
DE (1) DE602004002275T2 (ru)
DK (1) DK1583830T3 (ru)
EA (1) EA013225B1 (ru)
ES (1) ES2273202T3 (ru)
HK (1) HK1078896A1 (ru)
IL (1) IL169101A (ru)
MX (1) MXPA05006724A (ru)
NZ (1) NZ541458A (ru)
PT (1) PT1583830E (ru)
SI (1) SI1583830T1 (ru)
WO (1) WO2004061104A2 (ru)

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