EA200300991A1 - Продукт, содержащий иммобилизованную нуклеиновую кислоту, полученный способом с участием химерного олигонуклеотидного праймера, днк-полимеразы и эндонуклеазы - Google Patents

Продукт, содержащий иммобилизованную нуклеиновую кислоту, полученный способом с участием химерного олигонуклеотидного праймера, днк-полимеразы и эндонуклеазы

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Publication number
EA200300991A1
EA200300991A1 EA200300991A EA200300991A EA200300991A1 EA 200300991 A1 EA200300991 A1 EA 200300991A1 EA 200300991 A EA200300991 A EA 200300991A EA 200300991 A EA200300991 A EA 200300991A EA 200300991 A1 EA200300991 A1 EA 200300991A1
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EA
Eurasian Patent Office
Prior art keywords
nucleic acid
chimerous
endonuclease
participation
dna polymerase
Prior art date
Application number
EA200300991A
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English (en)
Other versions
EA005577B1 (ru
Inventor
Хироюки Мукаи
Юнко Ямамото
Осаму Такеда
Казуе Мияке
Такаси Уемори
Есими Сато
Марико Морияма
Харухиза Савараги
Митио Хагия
Киезо Асада
Икуносин Като
Original Assignee
Такара Био Инк.
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Application filed by Такара Био Инк. filed Critical Такара Био Инк.
Publication of EA200300991A1 publication Critical patent/EA200300991A1/ru
Publication of EA005577B1 publication Critical patent/EA005577B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

В изобретении раскрыты средство, применимое для удобного и эффективного способа амплификации последовательности нуклеиновой кислоты и способа выявления нуклеиновой кислоты - мишени, а именно химерные олигонуклеотидные праймеры, содержащие дезоксирибонуклеотид и рибонуклеотид, причём рибонуклеотид расположен на 3'-конце или со стороны 3'-конца праймера, а также применение ДНК-полимеразы с активностью вытеснения цепи и эндонуклеазы для осуществления указанных способов. Кроме того в изобретении раскрыт продукт, содержащий иммобилизованную нуклеиновую кислоту, размещенную в определенном порядке на предварительно заданном участке, причем способ получения продукта включает амплификацию последовательности нуклеиновой кислоты способом с участием химерного олигонуклеотидного праймера, ДНК-полимеразы с активностью вытеснения цепи и эндонуклеазы.Международная заявка была опубликована вместе с отчетом о международном поиске.
EA200300991A 1999-03-19 2000-03-14 Продукт, содержащий иммобилизованную нуклеиновую кислоту, полученный способом с участием химерного олигонуклеотидного праймера, днк-полимеразы и эндонуклеазы EA005577B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP7696699 1999-03-19
JP37003599 1999-12-27

Publications (2)

Publication Number Publication Date
EA200300991A1 true EA200300991A1 (ru) 2004-02-26
EA005577B1 EA005577B1 (ru) 2005-04-28

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EA200300991A EA005577B1 (ru) 1999-03-19 2000-03-14 Продукт, содержащий иммобилизованную нуклеиновую кислоту, полученный способом с участием химерного олигонуклеотидного праймера, днк-полимеразы и эндонуклеазы
EA200100990A EA004327B1 (ru) 1999-03-19 2000-03-14 Способ амплификации последовательности нуклеиновой кислоты

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EA200100990A EA004327B1 (ru) 1999-03-19 2000-03-14 Способ амплификации последовательности нуклеиновой кислоты

Country Status (11)

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EP (1) EP1167524B1 (ru)
JP (1) JP3433929B2 (ru)
KR (1) KR100682576B1 (ru)
CN (1) CN1227357C (ru)
AT (1) ATE362532T1 (ru)
AU (1) AU773536B2 (ru)
CA (1) CA2365135C (ru)
DE (1) DE60034878T2 (ru)
EA (2) EA005577B1 (ru)
TW (1) TWI259204B (ru)
WO (1) WO2000056877A1 (ru)

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Publication number Publication date
KR100682576B1 (ko) 2007-02-15
DE60034878T2 (de) 2008-01-17
WO2000056877A1 (fr) 2000-09-28
ATE362532T1 (de) 2007-06-15
EP1167524A4 (en) 2005-03-09
JP3433929B2 (ja) 2003-08-04
CN1227357C (zh) 2005-11-16
AU2944200A (en) 2000-10-09
CA2365135A1 (en) 2000-09-28
EA200100990A1 (ru) 2002-02-28
EA004327B1 (ru) 2004-04-29
DE60034878D1 (de) 2007-06-28
EP1167524B1 (en) 2007-05-16
CA2365135C (en) 2009-08-18
EA005577B1 (ru) 2005-04-28
KR20010104720A (ko) 2001-11-26
CN1350581A (zh) 2002-05-22
EP1167524A1 (en) 2002-01-02
AU773536B2 (en) 2004-05-27
TWI259204B (en) 2006-08-01

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