DK3059307T5 - Anvendelse af et laminin til differentiering af pluripotente celler i hepatocytcellelinier - Google Patents

Anvendelse af et laminin til differentiering af pluripotente celler i hepatocytcellelinier Download PDF

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Publication number
DK3059307T5
DK3059307T5 DK15305265.9T DK15305265T DK3059307T5 DK 3059307 T5 DK3059307 T5 DK 3059307T5 DK 15305265 T DK15305265 T DK 15305265T DK 3059307 T5 DK3059307 T5 DK 3059307T5
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Prior art keywords
laminin
differentiation
cell lines
pluripotent cells
hepatocyte cell
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DK15305265.9T
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DK3059307T4 (da
DK3059307T3 (da
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Angélique Fourrier
Tuan Huy Nguyen
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Inst Nat Sante Rech Med
Univ Nantes
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/98—Xeno-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/237—Oncostatin M [OSM]
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DK15305265.9T 2015-02-20 2015-02-20 Anvendelse af et laminin til differentiering af pluripotente celler i hepatocytcellelinier DK3059307T5 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP15305265.9A EP3059307B2 (en) 2015-02-20 2015-02-20 Use of a laminin for differentiating pluripotent cells into hepatocyte lineage cells

Publications (3)

Publication Number Publication Date
DK3059307T3 DK3059307T3 (da) 2019-01-14
DK3059307T4 DK3059307T4 (da) 2022-08-01
DK3059307T5 true DK3059307T5 (da) 2022-08-29

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DK15305265.9T DK3059307T5 (da) 2015-02-20 2015-02-20 Anvendelse af et laminin til differentiering af pluripotente celler i hepatocytcellelinier
DK16705202.6T DK3259345T3 (da) 2015-02-20 2016-02-19 Anvendelse af laminin til differentiering af pluripotente celler i hepatocyt-linie-celler

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DK16705202.6T DK3259345T3 (da) 2015-02-20 2016-02-19 Anvendelse af laminin til differentiering af pluripotente celler i hepatocyt-linie-celler

Country Status (17)

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US (2) US20180030415A1 (da)
EP (2) EP3059307B2 (da)
JP (2) JP6840084B2 (da)
CN (2) CN111548985A (da)
CA (1) CA2986830A1 (da)
CY (1) CY1121243T1 (da)
DK (2) DK3059307T5 (da)
ES (2) ES2703167T5 (da)
FI (1) FI3259345T3 (da)
HR (2) HRP20182119T4 (da)
HU (2) HUE041518T2 (da)
LT (2) LT3059307T (da)
PL (2) PL3059307T5 (da)
PT (2) PT3059307T (da)
RS (2) RS58070B2 (da)
SI (2) SI3059307T2 (da)
WO (1) WO2016131959A1 (da)

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US10226487B2 (en) * 2012-07-12 2019-03-12 Isletone Ab Derivation and self-renewal of lsl1+ cells and uses thereof
HUE041518T2 (hu) * 2015-02-20 2019-05-28 Inst Nat Sante Rech Med Laminin alkalmazása pluripotens sejtek hepatocita sejtvonalhoz tartozó sejtekké történõ differenciálódásához
DK3368657T3 (da) * 2015-10-30 2021-10-04 Biolamina Ab Fremgangsmåder til fremstilling af hepatocytter
WO2018019780A1 (en) * 2016-07-28 2018-02-01 F. Hoffmann-La Roche Ag Non-human primate induced pluripotent stem cell derived hepatocytes and uses thereof
BR112019009746A2 (pt) * 2016-11-16 2019-09-03 Cynata Therapeutics Ltd ensaio de células-tronco pluripotentes
US11530388B2 (en) 2017-02-14 2022-12-20 University of Pittsburgh—of the Commonwealth System of Higher Education Methods of engineering human induced pluripotent stem cells to produce liver tissue
JP6950887B2 (ja) * 2017-04-28 2021-10-13 米満　吉和 体外型の人工肝臓、及び体外型の人工肝臓用又は肝細胞培養用の器具
CN110914407A (zh) * 2017-05-05 2020-03-24 新加坡国立大学 人角质形成细胞的培养方法
US20210189346A1 (en) * 2018-05-16 2021-06-24 INSERM (Institut National de la Santé et de la Recherche Médicale) Method of culturing proliferative hepatocytes
US20210252070A1 (en) * 2018-06-29 2021-08-19 Platelet Biogenesis, Inc. Compositions for Drug Delivery and Methods of Use Thereof
US11227435B2 (en) 2018-08-13 2022-01-18 Magic Leap, Inc. Cross reality system
CN110982776B (zh) * 2018-09-30 2023-07-04 中国科学院分子细胞科学卓越创新中心 肝细胞的体外扩增培养方法及应用
US20210395679A1 (en) * 2018-11-09 2021-12-23 Children's Hospital Medical Center In vitro cell culture system for producing hepatocyte-like cells and uses thereof
CN111621467A (zh) * 2020-06-12 2020-09-04 青岛大学 一种诱导人脂肪间充质干细胞分化为肝脏样细胞的方法
CN115851578B (zh) * 2022-12-23 2023-11-21 华南理工大学 一种3d悬浮诱导持续扩增肝祖细胞类器官和/或肝细胞类器官的试剂盒及其应用

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US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
CA2457296A1 (en) * 2003-08-19 2005-02-19 Takahiro Ochiya Methods for inducing differentiation of pluripotent cells
JP2005095138A (ja) * 2003-08-19 2005-04-14 Effector Cell Institute Inc 多分化能を有する細胞の分化誘導方法
WO2006040763A2 (en) 2004-10-12 2006-04-20 Technion Research & Development Foundation Ltd. Isolated primate embryonic cells and methods of generating and using same
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CA2693481A1 (en) * 2007-07-20 2009-01-29 Cellartis Ab A novel population of hepatocytes derived via definitive endoderm (de-hep) from human blastocysts derived stem cells
WO2009057831A1 (ja) 2007-10-31 2009-05-07 Kyoto University 核初期化方法
JP2011516042A (ja) 2008-03-17 2011-05-26 へルムホルツ・ツェントルム・ミュンヘン−ドイチェス・フォルシュングスツェントルム・ヒューア・ゲズントハイト・ウント・ウムヴェルト（ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング） 部位特異的組み換えを用いて無ベクター誘導多能性幹（ｉＰＳ）細胞を作製するベクターおよびその方法
WO2010042490A1 (en) 2008-10-06 2010-04-15 Boston Medical Center Corporation A single lentiviral vector system for induced pluripotent (ips) stem cells derivation
JP2011010653A (ja) * 2009-06-05 2011-01-20 Kumamoto Univ 細胞の分化誘導方法
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US20130330825A1 (en) * 2012-06-07 2013-12-12 City Of Hope Attachment substrates for directed differentiation of human embryonic stem cells in culture
US10000554B2 (en) * 2012-12-28 2018-06-19 Osaka University Modified laminin containing collagen binding molecule and use thereof
CN105121632B (zh) * 2013-02-18 2021-08-10 大学健康网络 由多能干细胞生成肝细胞和胆管细胞的方法
SG11201507828VA (en) † 2013-04-08 2015-10-29 Nat Inst Of Biomedical Innovation Health And Nutrition Method for culturing hepatoblast-like cells and culture product thereof
HUE041518T2 (hu) 2015-02-20 2019-05-28 Inst Nat Sante Rech Med Laminin alkalmazása pluripotens sejtek hepatocita sejtvonalhoz tartozó sejtekké történõ differenciálódásához

Also Published As

Publication number Publication date
LT3059307T (lt) 2019-01-10
PL3059307T3 (pl) 2019-05-31
WO2016131959A1 (en) 2016-08-25
CN111548985A (zh) 2020-08-18
JP7132463B2 (ja) 2022-09-07
CA2986830A1 (en) 2016-08-25
SI3259345T1 (sl) 2023-08-31
HUE061457T2 (hu) 2023-06-28
RS64062B1 (sr) 2023-04-28
CN107532144A (zh) 2018-01-02
RS58070B2 (sr) 2022-10-31
LT3259345T (lt) 2023-03-27
HRP20182119T4 (hr) 2022-09-02
EP3259345A1 (en) 2017-12-27
JP6840084B2 (ja) 2021-03-17
PT3259345T (pt) 2023-03-15
PL3059307T5 (pl) 2023-04-03
US20180030415A1 (en) 2018-02-01
CY1121243T1 (el) 2020-05-29
US20240150721A1 (en) 2024-05-09
JP2021006054A (ja) 2021-01-21
RS58070B1 (sr) 2019-02-28
EP3059307B2 (en) 2022-05-04
DK3259345T3 (da) 2023-03-27
HRP20182119T1 (hr) 2019-03-08
SI3059307T2 (sl) 2022-08-31
HUE041518T2 (hu) 2019-05-28
PL3259345T3 (pl) 2023-05-02
HRP20230302T1 (hr) 2023-05-12
ES2940191T3 (es) 2023-05-04
EP3059307A1 (en) 2016-08-24
JP2018508207A (ja) 2018-03-29
ES2703167T3 (es) 2019-03-07
PT3059307T (pt) 2019-01-23
EP3259345B1 (en) 2023-02-08
DK3059307T4 (da) 2022-08-01
EP3059307B1 (en) 2018-10-17
FI3259345T3 (fi) 2023-04-03
DK3059307T3 (da) 2019-01-14
ES2703167T5 (es) 2022-09-30
SI3059307T1 (sl) 2019-03-29

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