DK3039122T3 - Generering af endokrine progenitorceller fra humane pluripotente stamceller under anvendelse af små molekyler - Google Patents
Generering af endokrine progenitorceller fra humane pluripotente stamceller under anvendelse af små molekyler Download PDFInfo
- Publication number
- DK3039122T3 DK3039122T3 DK14761822.7T DK14761822T DK3039122T3 DK 3039122 T3 DK3039122 T3 DK 3039122T3 DK 14761822 T DK14761822 T DK 14761822T DK 3039122 T3 DK3039122 T3 DK 3039122T3
- Authority
- DK
- Denmark
- Prior art keywords
- generation
- small molecules
- pluripotent stem
- human pluripotent
- endocrine progenitor
- Prior art date
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- C—CHEMISTRY; METALLURGY
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0613—Cells from endocrine organs
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0676—Pancreatic cells
- C12N5/0678—Stem cells; Progenitor cells; Precursor cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/15—Transforming growth factor beta (TGF-β)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/40—Regulators of development
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases (EC 2.)
- C12N2501/727—Kinases (EC 2.7.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/999—Small molecules not provided for elsewhere
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/22—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from pancreatic cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP13182440 | 2013-08-30 | ||
US201361875191P | 2013-09-09 | 2013-09-09 | |
PCT/EP2014/068393 WO2015028614A1 (en) | 2013-08-30 | 2014-08-29 | Generation of endocrine progenitor cells from human pluripotent stem cells using small molecules |
Publications (1)
Publication Number | Publication Date |
---|---|
DK3039122T3 true DK3039122T3 (da) | 2019-09-30 |
Family
ID=49080758
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK14761822.7T DK3039122T3 (da) | 2013-08-30 | 2014-08-29 | Generering af endokrine progenitorceller fra humane pluripotente stamceller under anvendelse af små molekyler |
Country Status (6)
Country | Link |
---|---|
US (2) | US20160208215A1 (da) |
EP (1) | EP3039122B1 (da) |
JP (1) | JP6636427B2 (da) |
CN (1) | CN105683361B (da) |
DK (1) | DK3039122T3 (da) |
WO (1) | WO2015028614A1 (da) |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR102580225B1 (ko) | 2013-06-11 | 2023-09-20 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | SC-β 세포 및 조성물 그리고 그 생성 방법 |
CN107614678B (zh) | 2014-12-18 | 2021-04-30 | 哈佛学院校长同事会 | 干细胞来源的β细胞的产生方法及其使用方法 |
US10190096B2 (en) | 2014-12-18 | 2019-01-29 | President And Fellows Of Harvard College | Methods for generating stem cell-derived β cells and uses thereof |
US10443042B2 (en) | 2014-12-18 | 2019-10-15 | President And Fellows Of Harvard College | Serum-free in vitro directed differentiation protocol for generating stem cell-derived beta cells and uses thereof |
WO2017144695A1 (en) * | 2016-02-24 | 2017-08-31 | Novo Nordisk A/S | Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors |
EP3681992A1 (en) * | 2017-09-11 | 2020-07-22 | Novo Nordisk A/S | Enrichment of nkx6.1 and c-peptide co-expressing cells derived in vitro from stem cells |
EP3710021A4 (en) | 2017-11-15 | 2021-08-11 | Semma Therapeutics, Inc. | COMPOSITIONS FOR THE MANUFACTURE OF ISLAND CELLS AND METHODS OF USE |
CN108165531A (zh) * | 2017-12-23 | 2018-06-15 | 淮北智淮科技有限公司 | 一种造血干细胞用的脐带血单核细胞培养方法 |
TWI818948B (zh) | 2018-01-18 | 2023-10-21 | 日商第一三共股份有限公司 | 啉酮衍生物 |
CA3108275A1 (en) | 2018-08-10 | 2020-02-13 | Vertex Pharmaceuticals Incorporated | Stem cell derived islet differentiation |
CA3110932A1 (en) | 2018-08-30 | 2020-03-05 | Novo Nordisk A/S | Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors |
US20220177849A1 (en) | 2019-04-08 | 2022-06-09 | Novo Nordisk A/S | Generation of pancreatic endoderm from stem cell derived definitive endoderm |
TW202115245A (zh) | 2019-06-27 | 2021-04-16 | 丹麥商諾佛 儂迪克股份有限公司 | 安全免疫隱形細胞 |
EP4204542A1 (en) | 2020-08-28 | 2023-07-05 | Novo Nordisk A/S | Method for screening in vitro population of stem cell derived beta like cells and novel markers thereof |
TW202242095A (zh) | 2020-12-18 | 2022-11-01 | 丹麥商諾佛 儂迪克股份有限公司 | 安全免疫隱形細胞 |
WO2022136215A1 (en) | 2020-12-21 | 2022-06-30 | Novo Nordisk A/S | Safe immuno-stealth cells |
WO2024008810A1 (en) | 2022-07-06 | 2024-01-11 | Novo Nordisk A/S | Differentiation of stem cells to pancreatic endocrine cells |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007103282A2 (en) * | 2006-03-02 | 2007-09-13 | Cythera, Inc. | Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production |
US7695965B2 (en) * | 2006-03-02 | 2010-04-13 | Cythera, Inc. | Methods of producing pancreatic hormones |
US20120021519A1 (en) * | 2008-09-19 | 2012-01-26 | Presidents And Fellows Of Harvard College | Efficient induction of pluripotent stem cells using small molecule compounds |
CN102272291B (zh) * | 2008-10-31 | 2018-01-16 | 詹森生物科技公司 | 人胚胎干细胞向胰腺内分泌谱系的分化 |
WO2010108126A2 (en) * | 2009-03-19 | 2010-09-23 | Fate Therapeutics, Inc. | Reprogramming compositions and methods of using the same |
EP2454271A4 (en) * | 2009-07-15 | 2015-08-12 | Univ California | PEPTIDES WITH CONTROLLABLE CELLULAR RECORDING |
CA2785966C (en) * | 2009-12-29 | 2020-10-27 | Takeda Pharmaceutical Company Limited | Method for manufacturing pancreatic-hormone-producing cells |
CN102093977A (zh) * | 2010-11-29 | 2011-06-15 | 中国人民解放军军事医学科学院基础医学研究所 | 一种诱导iPS细胞向心肌细胞分化的诱导因子及其应用 |
-
2014
- 2014-08-29 JP JP2016537310A patent/JP6636427B2/ja active Active
- 2014-08-29 DK DK14761822.7T patent/DK3039122T3/da active
- 2014-08-29 EP EP14761822.7A patent/EP3039122B1/en active Active
- 2014-08-29 CN CN201480047988.5A patent/CN105683361B/zh active Active
- 2014-08-29 US US14/913,604 patent/US20160208215A1/en not_active Abandoned
- 2014-08-29 WO PCT/EP2014/068393 patent/WO2015028614A1/en active Application Filing
-
2019
- 2019-08-20 US US16/546,018 patent/US20200002670A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
US20200002670A1 (en) | 2020-01-02 |
US20160208215A1 (en) | 2016-07-21 |
CN105683361B (zh) | 2021-04-02 |
EP3039122A1 (en) | 2016-07-06 |
JP6636427B2 (ja) | 2020-01-29 |
JP2016528908A (ja) | 2016-09-23 |
WO2015028614A1 (en) | 2015-03-05 |
EP3039122B1 (en) | 2019-07-17 |
CN105683361A (zh) | 2016-06-15 |
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