DK3037536T3 - Oligonukleotiderstatning for di-taggede og retnings biblioteker - Google Patents
Oligonukleotiderstatning for di-taggede og retnings biblioteker Download PDFInfo
- Publication number
- DK3037536T3 DK3037536T3 DK16155567.7T DK16155567T DK3037536T3 DK 3037536 T3 DK3037536 T3 DK 3037536T3 DK 16155567 T DK16155567 T DK 16155567T DK 3037536 T3 DK3037536 T3 DK 3037536T3
- Authority
- DK
- Denmark
- Prior art keywords
- oligonucleotid
- tagged
- directory
- libraries
- replacement
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161437451P | 2011-01-28 | 2011-01-28 | |
| US201161506777P | 2011-07-12 | 2011-07-12 | |
| EP12707675.0A EP2668273B9 (en) | 2011-01-28 | 2012-01-30 | Oligonucleotide replacement for di-tagged and directional libraries |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3037536T3 true DK3037536T3 (da) | 2020-01-13 |
Family
ID=45809570
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK12707675.0T DK2668273T3 (da) | 2011-01-28 | 2012-01-30 | Oligonukleotiderstatning for di-taggede og retningsbiblioteker |
| DK16155567.7T DK3037536T3 (da) | 2011-01-28 | 2012-01-30 | Oligonukleotiderstatning for di-taggede og retnings biblioteker |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK12707675.0T DK2668273T3 (da) | 2011-01-28 | 2012-01-30 | Oligonukleotiderstatning for di-taggede og retningsbiblioteker |
Country Status (7)
| Country | Link |
|---|---|
| US (4) | US9238671B2 (da) |
| EP (2) | EP2668273B9 (da) |
| AU (1) | AU2012211081B2 (da) |
| CA (1) | CA2821559C (da) |
| DK (2) | DK2668273T3 (da) |
| ES (2) | ES2568910T3 (da) |
| WO (1) | WO2012103545A1 (da) |
Families Citing this family (86)
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| US8383345B2 (en) | 2008-09-12 | 2013-02-26 | University Of Washington | Sequence tag directed subassembly of short sequencing reads into long sequencing reads |
| WO2010086622A1 (en) | 2009-01-30 | 2010-08-05 | Oxford Nanopore Technologies Limited | Adaptors for nucleic acid constructs in transmembrane sequencing |
| US9074251B2 (en) | 2011-02-10 | 2015-07-07 | Illumina, Inc. | Linking sequence reads using paired code tags |
| EP2670894B1 (en) | 2011-02-02 | 2017-11-29 | University Of Washington Through Its Center For Commercialization | Massively parallel continguity mapping |
| CA2843136C (en) | 2011-07-25 | 2020-12-29 | Oxford Nanopore Technologies Limited | Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores |
| NO2694769T3 (da) * | 2012-03-06 | 2018-03-03 | ||
| US11155860B2 (en) | 2012-07-19 | 2021-10-26 | Oxford Nanopore Technologies Ltd. | SSB method |
| US9388465B2 (en) | 2013-02-08 | 2016-07-12 | 10X Genomics, Inc. | Polynucleotide barcode generation |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10400280B2 (en) | 2012-08-14 | 2019-09-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| BR112015003354A8 (pt) | 2012-08-14 | 2018-01-16 | 10X Genomics Inc | métodos e composições de microcápsula |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CA2894694C (en) | 2012-12-14 | 2023-04-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9683230B2 (en) * | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
| WO2014135838A1 (en) | 2013-03-08 | 2014-09-12 | Oxford Nanopore Technologies Limited | Enzyme stalling method |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| AU2013382098B2 (en) | 2013-03-13 | 2019-02-07 | Illumina, Inc. | Methods and compositions for nucleic acid sequencing |
| CN111394426B (zh) | 2013-05-23 | 2024-05-10 | 斯坦福大学托管董事会 | 用于个人表观基因组学的至天然染色质的转座 |
| DK3036336T3 (da) | 2013-08-23 | 2020-11-02 | Ludwig Inst For Cancer Res Ltd | Fremgangsmåder og sammensætninger til cdna-syntese og enkeltcelle transkriptomprofilering under anvendelse af skabelonomskiftende reaktion |
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| JP6626830B2 (ja) * | 2013-11-07 | 2019-12-25 | アジレント・テクノロジーズ・インクＡｇｉｌｅｎｔ Ｔｅｃｈｎｏｌｏｇｉｅｓ， Ｉｎｃ． | Ｄｎａ操作のための複数のトランスポザーゼアダプター |
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| US20190194718A1 (en) * | 2014-01-14 | 2019-06-27 | Qiagen Gmbh | Generation of tagged dna fragments |
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| EP3889325A1 (en) | 2014-06-26 | 2021-10-06 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
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| CN106715713B (zh) * | 2014-09-12 | 2020-11-03 | 深圳华大智造科技有限公司 | 试剂盒及其在核酸测序中的用途 |
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| JP2017534297A (ja) * | 2014-10-14 | 2017-11-24 | ビージーアイ シェンヂェン カンパニー リミテッドＢｇｉ Ｓｈｅｎｚｈｅｎ Ｃｏ．， Ｌｉｍｉｔｅｄ | トランスポゼースによる核酸の切断及びリンカー付加の方法及び試薬 |
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2012
- 2012-01-30 WO PCT/US2012/023139 patent/WO2012103545A1/en active Application Filing
- 2012-01-30 DK DK12707675.0T patent/DK2668273T3/da active
- 2012-01-30 US US13/979,346 patent/US9238671B2/en active Active
- 2012-01-30 EP EP12707675.0A patent/EP2668273B9/en active Active
- 2012-01-30 DK DK16155567.7T patent/DK3037536T3/da active
- 2012-01-30 AU AU2012211081A patent/AU2012211081B2/en active Active
- 2012-01-30 ES ES12707675.0T patent/ES2568910T3/es active Active
- 2012-01-30 CA CA2821559A patent/CA2821559C/en active Active
- 2012-01-30 ES ES16155567T patent/ES2762866T3/es active Active
- 2012-01-30 EP EP16155567.7A patent/EP3037536B1/en active Active
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2015
- 2015-12-08 US US14/963,021 patent/US10287574B2/en active Active
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2019
- 2019-04-01 US US16/372,207 patent/US11008567B2/en active Active
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2021
- 2021-04-29 US US17/244,365 patent/US20210261951A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20190225960A1 (en) | 2019-07-25 |
| US20140031261A1 (en) | 2014-01-30 |
| ES2568910T3 (es) | 2016-05-05 |
| US11008567B2 (en) | 2021-05-18 |
| EP2668273B1 (en) | 2016-04-06 |
| CA2821559A1 (en) | 2012-08-02 |
| US10287574B2 (en) | 2019-05-14 |
| ES2762866T3 (es) | 2020-05-26 |
| EP2668273B9 (en) | 2017-03-29 |
| DK2668273T3 (da) | 2016-04-18 |
| WO2012103545A1 (en) | 2012-08-02 |
| EP3037536A1 (en) | 2016-06-29 |
| US20160090591A1 (en) | 2016-03-31 |
| US20210261951A1 (en) | 2021-08-26 |
| EP3037536B1 (en) | 2019-11-27 |
| AU2012211081B2 (en) | 2016-02-04 |
| CA2821559C (en) | 2017-01-31 |
| US9238671B2 (en) | 2016-01-19 |
| AU2012211081A1 (en) | 2013-07-04 |
| EP2668273A1 (en) | 2013-12-04 |
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