DK3036336T3 - Fremgangsmåder og sammensætninger til cdna-syntese og enkeltcelle transkriptomprofilering under anvendelse af skabelonomskiftende reaktion - Google Patents

Fremgangsmåder og sammensætninger til cdna-syntese og enkeltcelle transkriptomprofilering under anvendelse af skabelonomskiftende reaktion Download PDF

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DK3036336T3
DK3036336T3 DK14837396.2T DK14837396T DK3036336T3 DK 3036336 T3 DK3036336 T3 DK 3036336T3 DK 14837396 T DK14837396 T DK 14837396T DK 3036336 T3 DK3036336 T3 DK 3036336T3
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propholfing
compositions
methods
single cell
cdna synthesis
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DK14837396.2T
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Rickard Sandberg
Simone Picelli
Omid R Faridani
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Ludwig Inst For Cancer Res Ltd
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
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    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Chemical & Material Sciences (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Biomedical Technology (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
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  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK14837396.2T 2013-08-23 2014-08-22 Fremgangsmåder og sammensætninger til cdna-syntese og enkeltcelle transkriptomprofilering under anvendelse af skabelonomskiftende reaktion DK3036336T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361869220P 2013-08-23 2013-08-23
PCT/US2014/052233 WO2015027135A1 (en) 2013-08-23 2014-08-22 Methods and compositions for cdna synthesis and single-cell transcriptome profiling using template switching reaction

Publications (1)

Publication Number Publication Date
DK3036336T3 true DK3036336T3 (da) 2020-11-02

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DK14837396.2T DK3036336T3 (da) 2013-08-23 2014-08-22 Fremgangsmåder og sammensætninger til cdna-syntese og enkeltcelle transkriptomprofilering under anvendelse af skabelonomskiftende reaktion

Country Status (8)

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US (2) US10266894B2 (da)
EP (1) EP3036336B1 (da)
JP (2) JP6336080B2 (da)
CN (2) CN105579587A (da)
CA (1) CA2921603C (da)
DK (1) DK3036336T3 (da)
ES (1) ES2827307T3 (da)
WO (1) WO2015027135A1 (da)

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DK3036336T3 (da) * 2013-08-23 2020-11-02 Ludwig Inst For Cancer Res Ltd Fremgangsmåder og sammensætninger til cdna-syntese og enkeltcelle transkriptomprofilering under anvendelse af skabelonomskiftende reaktion
PL3089822T3 (pl) * 2013-12-30 2022-09-19 Atreca, Inc. Analiza kwasów nukleinowych powiązanych z pojedynczymi komórkami z użyciem kodów kreskowych kwasów nukleinowych
US10870848B2 (en) 2015-09-15 2020-12-22 Takara Bio Usa, Inc. Methods for preparing a next generation sequencing (NGS) library from a ribonucleic acid (RNA) sample and compositions for practicing the same
AU2016348439B2 (en) 2015-11-04 2023-03-09 Atreca, Inc. Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
US11371087B2 (en) 2016-06-10 2022-06-28 Takara Bio Usa, Inc. Methods and compositions employing blocked primers
CN106498040B (zh) * 2016-10-12 2018-10-23 浙江大学 一种分子标记微珠及基于该分子标记微珠的高通量单细胞测序方法
EP3538662B1 (en) * 2016-11-10 2021-12-01 Takara Bio USA, Inc. Methods of producing amplified double stranded deoxyribonucleic acids and compositions and kits for use therein
KR20230013167A (ko) 2016-11-11 2023-01-26 바이오 래드 래버러토리스 인코오포레이티드 핵산 샘플을 프로세싱하는 방법
CN106868005B (zh) * 2017-03-17 2020-07-21 中山大学附属肿瘤医院 一种用于高效快速扩增cDNA末端的锚定引物及扩增方法
AU2018328324A1 (en) * 2017-09-11 2020-04-30 Ludwig Institute For Cancer Research Ltd Selective labeling of 5-methylcytosine in circulating cell-free DNA
CN108103055B (zh) * 2018-01-09 2021-05-28 序康医疗科技（苏州）有限公司 一种单细胞rna逆转录与文库构建的方法
EP3746570B1 (en) 2018-02-02 2022-11-30 The United States of America, as represented by the Secretary, Department of Health and Human Services Random amplification methods for extremely low input nucleic acids
WO2020036926A1 (en) 2018-08-17 2020-02-20 Cellecta, Inc. Multiplex preparation of barcoded gene specific dna fragments
US20220033811A1 (en) 2018-12-28 2022-02-03 Biobloxx Ab Method and kit for preparing complementary dna
CN113811610A (zh) * 2019-05-09 2021-12-17 加利福尼亚太平洋生物科学股份有限公司 用于改进cDNA合成的组合物和方法
US20220356462A1 (en) 2019-07-11 2022-11-10 Tokyo University Of Science Foundation Method for amplifying nucleic acid using solid-phase carrier
US20230193254A1 (en) * 2020-05-20 2023-06-22 Chan Zuckerberg Biohub, Inc. Total rna profiling of biological samples and single cells
CN117247990A (zh) * 2022-06-17 2023-12-19 复旦大学 基于拓扑捕获的rna样本处理***
CN116497105B (zh) * 2023-06-28 2023-09-29 浙江大学 基于末端转移酶的单细胞转录组测序试剂盒及测序方法

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US5962271A (en) * 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
IL145422A0 (en) 1999-03-18 2002-06-30 Exiqon As One step sample preparation and detection of nucleic acids in complex in biological samples
EP3002337B1 (en) 2009-03-30 2018-10-24 Illumina, Inc. Gene expression analysis in single cells
DK3037536T3 (da) 2011-01-28 2020-01-13 Illumina Inc Oligonukleotiderstatning for di-taggede og retnings biblioteker
EP2670894B1 (en) 2011-02-02 2017-11-29 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
WO2012129363A2 (en) * 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
DK3036336T3 (da) * 2013-08-23 2020-11-02 Ludwig Inst For Cancer Res Ltd Fremgangsmåder og sammensætninger til cdna-syntese og enkeltcelle transkriptomprofilering under anvendelse af skabelonomskiftende reaktion

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Publication number Publication date
CN115558661A (zh) 2023-01-03
JP6336080B2 (ja) 2018-06-06
CN105579587A (zh) 2016-05-11
EP3036336A1 (en) 2016-06-29
CA2921603A1 (en) 2015-02-26
EP3036336B1 (en) 2020-09-30
ES2827307T3 (es) 2021-05-20
US20190264284A1 (en) 2019-08-29
WO2015027135A1 (en) 2015-02-26
CA2921603C (en) 2019-11-05
JP6603699B2 (ja) 2019-11-06
US10266894B2 (en) 2019-04-23
JP2018088924A (ja) 2018-06-14
US20160258016A1 (en) 2016-09-08
EP3036336A4 (en) 2017-06-28
JP2016527919A (ja) 2016-09-15

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