DK2691411T3 - Buffersystem til proteinoprensning - Google Patents

Buffersystem til proteinoprensning Download PDF

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Publication number
DK2691411T3
DK2691411T3 DK12764025.8T DK12764025T DK2691411T3 DK 2691411 T3 DK2691411 T3 DK 2691411T3 DK 12764025 T DK12764025 T DK 12764025T DK 2691411 T3 DK2691411 T3 DK 2691411T3
Authority
DK
Denmark
Prior art keywords
protein purification
buffer system
purification buffer
protein
purification
Prior art date
Application number
DK12764025.8T
Other languages
English (en)
Inventor
Kent E Goklen
Eric J Suda
Antonio Raul Ubiera
Original Assignee
Glaxosmithkline Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=46931905&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK2691411(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Glaxosmithkline Llc filed Critical Glaxosmithkline Llc
Application granted granted Critical
Publication of DK2691411T3 publication Critical patent/DK2691411T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3847—Multimodal interactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
DK12764025.8T 2011-03-29 2012-03-29 Buffersystem til proteinoprensning DK2691411T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161468814P 2011-03-29 2011-03-29
PCT/US2012/031076 WO2012135415A1 (en) 2011-03-29 2012-03-29 Buffer system for protein purification

Publications (1)

Publication Number Publication Date
DK2691411T3 true DK2691411T3 (da) 2020-05-11

Family

ID=46931905

Family Applications (1)

Application Number Title Priority Date Filing Date
DK12764025.8T DK2691411T3 (da) 2011-03-29 2012-03-29 Buffersystem til proteinoprensning

Country Status (15)

Country Link
US (2) US9624261B2 (da)
EP (1) EP2691411B1 (da)
JP (1) JP6147726B2 (da)
KR (1) KR102058254B1 (da)
CN (2) CN103547589A (da)
AU (1) AU2012236486B2 (da)
BR (1) BR112013024933A2 (da)
CA (1) CA2831787A1 (da)
DK (1) DK2691411T3 (da)
EA (1) EA028326B1 (da)
ES (1) ES2784628T3 (da)
IL (1) IL228610B (da)
MY (1) MY162570A (da)
SG (1) SG193545A1 (da)
WO (1) WO2012135415A1 (da)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG193545A1 (en) 2011-03-29 2013-11-29 Glaxosmithkline Llc Buffer system for protein purification
WO2013075740A1 (en) * 2011-11-23 2013-05-30 Sanofi Antibody purification method
BR112015022321A2 (pt) * 2013-03-15 2017-07-18 Glaxosmithkline Ip No 2 Ltd método para purificação de uma proteína
JP6463734B2 (ja) * 2013-05-06 2019-02-06 サノフイＳａｎｏｆｉ 抗体を精製するための連続的多工程法
CA2922832A1 (en) * 2013-09-05 2015-03-12 Genentech, Inc. Method for chromatography reuse
US11566082B2 (en) 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
EP3252158A4 (en) 2015-01-26 2018-07-18 Kaneka Corporation Mutant immunoglobulin kappa chain variable region-binding peptide
JPWO2016121701A1 (ja) 2015-01-26 2017-11-09 株式会社カネカ 免疫グロブリンκ鎖可変領域含有タンパク質精製用アフィニティー分離マトリックス
WO2017156355A1 (en) * 2016-03-11 2017-09-14 Boehringer Ingelheim International Gmbh Methods for continuously inactivating a virus during manufacture of a protein
WO2017195638A1 (ja) 2016-05-09 2017-11-16 株式会社カネカ 抗体またはκ鎖可変領域含有抗体断片の精製方法
US10889615B2 (en) 2016-05-11 2021-01-12 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
US10513537B2 (en) 2016-05-11 2019-12-24 Ge Healthcare Bioprocess R&D Ab Separation matrix
US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
US11753438B2 (en) 2016-05-11 2023-09-12 Cytiva Bioprocess R&D Ab Method of cleaning and/or sanitizing a separation matrix
WO2017194592A1 (en) 2016-05-11 2017-11-16 Ge Healthcare Bioprocess R&D Ab Method of storing a separation matrix
US10654887B2 (en) 2016-05-11 2020-05-19 Ge Healthcare Bio-Process R&D Ab Separation matrix
US10730908B2 (en) 2016-05-11 2020-08-04 Ge Healthcare Bioprocess R&D Ab Separation method
KR102489451B1 (ko) 2016-09-07 2023-01-16 글락소스미스클라인 인털렉츄얼 프로퍼티 디벨로프먼트 리미티드 항체를 정제하는 방법
KR20190047376A (ko) * 2017-10-27 2019-05-08 주식회사 녹십자 개선된 면역글로불린의 정제방법
KR20190092951A (ko) * 2018-01-31 2019-08-08 씨제이제일제당 (주) 연속식 크로마토그래피 공정을 이용한 천연 l-시스테인 결정의 제조 방법
CN111902720A (zh) 2018-03-21 2020-11-06 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
EP3791172B1 (en) * 2018-05-08 2023-06-28 Waters Technologies Corporation Methods, compositions and kits useful for ph gradient cation exchange chromatography
KR20210021542A (ko) * 2018-06-19 2021-02-26 브리스톨-마이어스 스큅 컴퍼니 크로마토그래피를 사용하여 단백질을 정제하는 방법
RU2693251C1 (ru) * 2018-11-12 2019-07-01 Федеральное государственное бюджетное учреждение науки Институт химической биологии и фундаментальной медицины Сибирского отделения Российской академии наук (ИХБФМ СО РАН) Способ выделения и очистки рекомбинантного белка, аналога фрагмента каппа-казеина человека, обладающего цитотоксической активностью по отношению к раковым клеткам человека
CN110437302A (zh) * 2019-07-19 2019-11-12 上海药明生物技术有限公司 基于商业蛋白层析***的半自动化高通量纯化方法
WO2022172284A1 (en) * 2021-02-11 2022-08-18 Gennova Biopharmaceuticals Ltd. Purification of a therapeutic protein
CN113980092B (zh) * 2021-12-09 2024-05-14 上海药明生物技术有限公司 一种蛋白质亲和纯化方法
WO2023194837A1 (en) * 2022-04-04 2023-10-12 Intas Pharmaceuticals Ltd. Multi-component buffer system for purification of antibodies

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57106673A (en) 1980-12-24 1982-07-02 Chugai Pharmaceut Co Ltd Dibenzo(b,f)(1,4)oxazepin derivative
US4634678A (en) * 1982-12-13 1987-01-06 Molecular Genetics Research And Development Limited Partnership Plasmid cloning and expression vectors for use in microorganisms
GB8607679D0 (en) 1986-03-27 1986-04-30 Winter G P Recombinant dna product
FR2597605B1 (fr) * 1986-04-16 1989-06-23 Merieux Inst Nouveau materiau pour chromatographie d'affinite et son application a la separation et a la purification des antigenes proteiques des bacteries du genre bordetella
US4981952A (en) 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
IT1262899B (it) 1992-03-27 1996-07-22 Sclavo Spa Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano
US5429746A (en) 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
JPH09121858A (ja) 1995-11-06 1997-05-13 Fusanori Hamashima 免疫的寛容誘導剤
CA2249551A1 (en) * 1996-03-19 1997-09-25 Proscript, Inc. A kinase capable of site-specific phosphorylation of i.kappa.b.alpha.
IL127127A0 (en) 1998-11-18 1999-09-22 Peptor Ltd Small functional units of antibody heavy chain variable regions
GB9902919D0 (en) * 1999-02-09 1999-03-31 Isis Innovation Modulator of inflammation
US7101982B2 (en) 2001-03-30 2006-09-05 Immunex Corporation Control of ph transitions during chromatography
AU2002319544B2 (en) 2001-08-10 2008-07-10 Aberdeen University Antigen binding domains from fish
IL145035A0 (en) * 2001-08-21 2002-06-30 Yeda Res & Dev Molecular linkers suitable for crystallization and structural analysis of molecules of interest and method of using same
SI21102A (sl) 2001-12-19 2003-06-30 LEK, tovarna farmacevtskih in kemi�nih izdelkov, d.d. Postopek za izolacijo biološko aktivnega granulocitne kolonije stimulirajočega dejavnika
ATE417858T1 (de) * 2002-02-05 2009-01-15 Genentech Inc Proteinaufreinigung
DE60228460D1 (de) 2002-12-13 2008-10-02 Bioceuticals Arzneimittel Ag Verfahren zur Herstellung und Reinigung von Erythropoietin
AU2004291341B2 (en) 2003-11-05 2010-08-05 Ares Trading S.A. Process for the purification of IL-18 binding protein
SE0400886D0 (sv) 2004-04-02 2004-04-02 Amersham Biosciences Ab Process of purification
GB0417487D0 (en) * 2004-08-05 2004-09-08 Novartis Ag Organic compound
US20060118492A1 (en) 2004-12-08 2006-06-08 Chia-Hui Shieh Integrated column, related system and method for liquid chromatography
SE0403057D0 (sv) * 2004-12-14 2004-12-14 Amersham Biosciences Ab Purification of immunoglobulins
CN104926938B (zh) * 2005-06-17 2019-06-04 惠氏有限责任公司 纯化含Fc区蛋白的方法
WO2009017491A1 (en) * 2006-06-14 2009-02-05 Smithkline Beecham Corporation Methods for purifying antibodies using ceramic hydroxyapatite
EP2061802A1 (en) 2006-08-28 2009-05-27 Ares Trading S.A. Process for the purification of fc-fusion proteins
FI2061803T4 (fi) 2006-08-28 2023-03-14 Process for the purification of fc-containing proteins
AU2008209404B2 (en) * 2007-01-22 2012-08-16 Genentech, Inc. Polyelectrolyte precipitation and purification of antibodies
DK2125866T3 (da) 2007-02-28 2013-07-29 Baxter Int Fremgangsmåde til rensning af rekombinant blodkoagulationsfaktor IX, som er beriget med sulfaterede og/eller phosphorylerede molekyler
SG175597A1 (en) * 2007-10-30 2011-11-28 Genentech Inc Antibody purification by cation exchange chromatography
CN101220081B (zh) * 2008-01-28 2011-01-05 江苏吴中医药集团有限公司苏州中凯生物制药厂 重组蛋白提取纯化方法
WO2009144775A1 (ja) * 2008-05-29 2009-12-03 Toto株式会社 抗腫瘍剤
WO2010063717A1 (en) 2008-12-02 2010-06-10 Novo Nordisk A/S Polypeptide purification
EP2360183B1 (en) 2008-12-19 2016-07-27 Takeda Pharmaceutical Company Limited Antibody purification method by mixed mode chromatography utilizing an arginine-containing loading solution
WO2010127069A1 (en) * 2009-04-29 2010-11-04 Schering Corporation Antibody purification
WO2011015919A1 (en) 2009-08-03 2011-02-10 Avesthagen Limited A highly efficient process of purification and production of recombinant infliximab
SG193545A1 (en) * 2011-03-29 2013-11-29 Glaxosmithkline Llc Buffer system for protein purification

Also Published As

Publication number Publication date
EA201391402A1 (ru) 2014-01-30
KR20140022849A (ko) 2014-02-25
US20140018525A1 (en) 2014-01-16
ES2784628T3 (es) 2020-09-29
JP6147726B2 (ja) 2017-06-14
CN107915769A (zh) 2018-04-17
CN103547589A (zh) 2014-01-29
CA2831787A1 (en) 2012-10-04
AU2012236486A1 (en) 2013-10-17
EA028326B1 (ru) 2017-11-30
MY162570A (en) 2017-06-30
BR112013024933A2 (pt) 2016-09-06
US9624261B2 (en) 2017-04-18
EP2691411A4 (en) 2014-09-10
KR102058254B1 (ko) 2019-12-20
US20170174721A1 (en) 2017-06-22
JP2014509651A (ja) 2014-04-21
EP2691411A1 (en) 2014-02-05
AU2012236486B2 (en) 2016-06-23
EP2691411B1 (en) 2020-02-26
SG193545A1 (en) 2013-11-29
US9868761B2 (en) 2018-01-16
IL228610A0 (en) 2013-12-31
WO2012135415A1 (en) 2012-10-04
IL228610B (en) 2018-06-28

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