DK2451954T3 - Modificeret promotor - Google Patents

Modificeret promotor Download PDF

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Publication number
DK2451954T3
DK2451954T3 DK10773723.1T DK10773723T DK2451954T3 DK 2451954 T3 DK2451954 T3 DK 2451954T3 DK 10773723 T DK10773723 T DK 10773723T DK 2451954 T3 DK2451954 T3 DK 2451954T3
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DK
Denmark
Prior art keywords
promoter
asn
glu
ala
asp
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DK10773723.1T
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English (en)
Inventor
Akihito Kawahara
Hiroshi Kodama
Katsutoshi Ara
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Kao Corp
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Publication of DK2451954T3 publication Critical patent/DK2451954T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (6)

1. Fremgangsmåde til fremstilling afen modificeret promotor, hvilken fremgangsmåde består i et trin med deletering af én eller begge af SEQ ID NO: 1 eller SEQ ID NO: 2 i 5 nucleotidsekvensen for promotoren afledes fra en bakterie hørende til slægten Bacillus, hvor promotoren afledt fra en bakterie hørende til slægten Bacillus er: (a) en promotor for et alkalisk cellulasegen fra Bacillus sp. KSM-64-stammen (FERM BP-2886) bestående af en nucleotidsekvens repræsenteret ved nucleo- 10 tidnumrene 1 til 609 i SEQ ID NO: 3; (b) en promotor for et alkalisk cellulasegen fra Bacillus sp. KSM-S237-stammen (FERM BP-7875) bestående af en nucleotidsekvens repræsenteret ved nucleo-tidnumrene 1 til 572 i SEQ ID NO: 4; eller (c) en promotor, som indeholder en nucleotidsekvens, som har en sekvens- 15 identitet på 90% eller mere i forhold til promotoren for det alkaliske cellulasegen bestående af en nucleotidsekvens repræsenteret ved nucleotidnumrene 1 til 609 i SEQ ID NO: 3, eller i forhold til promotoren for det alkaliske cellulasegen, som består af en nucleotidsekvens repræsenteret ved nucleotidnumrene 1 til 572 i SEQ ID NO: 4, og som har en cre-lignende sekvens repræsenteret ved SEQ ID 20 NO: 1 og/ellerSEQ ID NO: 2.
2. Modificeret promotor afledt fra en bakterie hørende til slægten Bacillus, hvor promotoren afledt fra bakterien hørende til slæften Bacillus er: 25 (a) en promotor for et alkalisk cellulasegen fra Bacillus sp. KSM-64-stammen (FERM BP-2886) bestående af en nucleotidsekvens repræsenteret ved nucleotidnumrene 1 til 609 i SEQ ID NO: 3 med en deletering af SEQ ID NO: 1 og/eller SEQ ID NO: 2; (b) en promotor for et alkalisk cellulasegen fra Bacillus sp. KSM-S237-stammen 30 (FERM BP-7875) bestående af en nucleotidsekvens repræsenterete ved nucleotidnumrene 1 til 572 i SEQ ID NO: 4, med en deletering af SEQ ID NO: 1 og/eller SEQ ID NO: 2; eller (c) en promotor bestående af en nucleotidsekvens, som har en sekvensidentitet på 90% eller mere i forhold til promotoren for et alkalisk cellulasegen bestående af 35 en nucleotidsekvens repræsenteret ved nucleotidnumrene 1 til 609 i SEQ ID NO: 3, eller i forhold til en promotor for det alkaliske cellulosegen bestående af nucleo-tidsekvensen repræsenteret ved nucleotidnumrene 1 til 572 i SEQ ID NO: 4 og som har en deletion af den cre-lignende sekvens repræsenteret ved SEQ ID NO: 1 og/eller SEQ ID NO: 2 5
3. Ekspressionsvektor omfattende en modificeret promotor ifølge krav 2.
4. Ekspressionsvektor ifølge krav 3, hvor den modificerede promotor er funktionsmæssigt forbundet med et gen opstrøms derfor, som koder for et genprodukt af 10 interesse.
5. Transformant omfattende den modificerede promotor ifølge krav 2, hvor den modificerede promotor er funktionsmæssigt forbundet med et opstrøms derfor, som koder for et genprodukt af interesse. 15
6. Fremgangsmåde til fremstilling af et genprodukt af interesse, hvilken fremgangsmåde omfatter anvendelse af en transformant ifølge krav 5. 20
DK10773723.1T 2009-10-21 2010-10-18 Modificeret promotor DK2451954T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2009242178 2009-10-21
JP2010224065A JP5735775B2 (ja) 2009-10-21 2010-10-01 改変プロモーター
PCT/JP2010/068783 WO2011049227A1 (en) 2009-10-21 2010-10-18 Modified promoter

Publications (1)

Publication Number Publication Date
DK2451954T3 true DK2451954T3 (da) 2018-11-05

Family

ID=43589658

Family Applications (1)

Application Number Title Priority Date Filing Date
DK10773723.1T DK2451954T3 (da) 2009-10-21 2010-10-18 Modificeret promotor

Country Status (8)

Country Link
US (1) US9029519B2 (da)
EP (1) EP2451954B1 (da)
JP (1) JP5735775B2 (da)
CN (1) CN102575253A (da)
AU (1) AU2010308865B2 (da)
BR (1) BR112012005263A2 (da)
DK (1) DK2451954T3 (da)
WO (1) WO2011049227A1 (da)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016134081A1 (en) 2015-02-18 2016-08-25 Iowa State University Research Foundation, Inc. Modification of transcriptional repressor binding site in nf-yc4 promoter for increased protein content and resistance to stress
WO2017022583A1 (ja) * 2015-08-06 2017-02-09 花王株式会社 新規プロモーター
CN107142259A (zh) * 2017-05-25 2017-09-08 中国科学院上海高等研究院 一种表达外源基因的启动子及其应用
JP7218090B2 (ja) 2018-01-12 2023-02-06 花王株式会社 タンパク質の製造方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000078981A (ja) 1998-07-08 2000-03-21 Kao Corp Ｄｎａ断片
JP2000210081A (ja) 1999-01-21 2000-08-02 Kao Corp 耐熱性アルカリセルラ―ゼ遺伝子
DE60127661T2 (de) * 2000-04-13 2007-12-06 Mark Aaron Jupiter Emalfarb Expression regulierende sequenzen von schimmelpilz chrysosporium
JP4426716B2 (ja) 2000-10-11 2010-03-03 花王株式会社 高生産性α−アミラーゼ
WO2005085441A1 (ja) * 2004-03-05 2005-09-15 Kao Corporation 改変プロモーター
CN101978056B (zh) 2008-03-25 2013-05-22 花王株式会社 质粒载体

Also Published As

Publication number Publication date
US20120183998A1 (en) 2012-07-19
WO2011049227A1 (en) 2011-04-28
US9029519B2 (en) 2015-05-12
EP2451954A1 (en) 2012-05-16
BR112012005263A2 (pt) 2020-11-03
EP2451954B1 (en) 2018-08-08
JP5735775B2 (ja) 2015-06-17
JP2011103875A (ja) 2011-06-02
AU2010308865A1 (en) 2012-05-17
AU2010308865B2 (en) 2014-08-21
CN102575253A (zh) 2012-07-11

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