DK1957636T3 - Pdx1-udtrykkende dorsal og ventral for-tarm-endoderm - Google Patents

Pdx1-udtrykkende dorsal og ventral for-tarm-endoderm Download PDF

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DK1957636T3
DK1957636T3 DK06827129.5T DK06827129T DK1957636T3 DK 1957636 T3 DK1957636 T3 DK 1957636T3 DK 06827129 T DK06827129 T DK 06827129T DK 1957636 T3 DK1957636 T3 DK 1957636T3
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cells
pdx1
cell
positive
expression
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DK06827129.5T
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Kevin Allen D'amour
Susan Eliazer
Emmanuel E Baetge
Alan D Agulnick
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Viacyte Inc
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Claims (9)

1. En in v/fro-cel lekultur omfattende humane celler, hvor mindst 10% af de humane celler er endodermceller der udtrykker en markør valgt fra gruppen bestående af ONECUT2, SOX9 og PROX1 og som udtrykker det pankreatiske-duodenale homeobox faktor-1 (PDXl)-protein.
2. Cellekulturen ifølge krav 1, yderligere omfatter et retinoid.
3. Cellekulturen ifølge krav 2, hvor retinoidet omfatter retinonsyre (RA).
4. Cellekulturen ifølge et hvilket som helst af de foregående krav, hvor cellekulturen i det væsentlige er fri for celler valgt fra gruppen bestående af viscerale endodermceller, parietale endodermceller og neurale celler.
5. Cellekulturen ifølge et hvilket som helst af de foregående krav, hvor endodermcellerne er multipotente celler der yderligere kan differentiere til celler afledt fra for-tarmen.
6. Fremgangsmåde til fremstilling af PDXl-positive endodermceller, hvilken fremgangsmåde omfatter at: (a) dyrke en opnået population af celler omfattende humane pluripotente celler i tilstedeværelse af en exogen TGFp-superfamilievækstfaktor, derved producere en population af celler omfattende PDXl-negative definitive endodermceller; (b) dyrke cellerne fra trin (a) i tilstedeværelse af en exogen FGF- familievækstfaktor valgt fra gruppen bestående af FGF10 og FGF7, derved producere fortarm-endotermceller; og (c) dyrke cellerne fra trin (b) i tilstedeværelse af et retinoid, derved producere multipotente PDXl-positive endodermceller.
7. Fremgangsmåden ifølge krav 6, hvor TGFp-superfamilievækstfaktoren omfatter activin A, hvor FGF-familievækstfaktoren omfatter FGF-7 eller hvor retinoidet omfatter retinonsyre (RA).
8. Fremgangsmåden ifølge krav 6 eller krav 7, hvor den exogene TGFp-superfamilievækstfaktor er fjernet før trin (b).
9. Fremgangsmåden ifølge et hvilket som helst af kravene 6-8, hvor ekspressionen af PDX1 er større end ekspressionen af en markør valgt fra gruppen bestående af alfa-fetoprotein (AFP), SOX7, SOX1, ZIC1 og NFM i hvilken de PDXl-positive endodermceller som bestemt ved kvantitativ polymerasekædereaktion eller ved immunocytokemi.
DK06827129.5T 2005-10-27 2006-10-27 Pdx1-udtrykkende dorsal og ventral for-tarm-endoderm DK1957636T3 (da)

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US73091705P 2005-10-27 2005-10-27
PCT/US2006/042413 WO2007051038A2 (en) 2005-10-27 2006-10-27 Pdx1-expressing dorsal and ventral foregut endoderm

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DK13171221.8T DK2674485T3 (da) 2005-10-27 2006-10-27 Pdx-1 udtrykkende dorsal og ventral fortarm endoderm

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US (6) US20070122905A1 (da)
EP (3) EP3584311A1 (da)
JP (5) JP5404047B2 (da)
CN (1) CN101351547A (da)
AU (1) AU2006305879B2 (da)
CA (2) CA2869687C (da)
DK (2) DK1957636T3 (da)
ES (2) ES2743202T3 (da)
PL (1) PL1957636T3 (da)
WO (1) WO2007051038A2 (da)

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