BR0311360A - métodos para expansão e transdiferenciação in vitro de células acinares pancreáticas humanas em células produtoras de insulina - Google Patents
métodos para expansão e transdiferenciação in vitro de células acinares pancreáticas humanas em células produtoras de insulinaInfo
- Publication number
- BR0311360A BR0311360A BR0311360A BR0311360A BR0311360A BR 0311360 A BR0311360 A BR 0311360A BR 0311360 A BR0311360 A BR 0311360A BR 0311360 A BR0311360 A BR 0311360A BR 0311360 A BR0311360 A BR 0311360A
- Authority
- BR
- Brazil
- Prior art keywords
- cells
- methods
- insulin producing
- transdifferentiation
- human pancreatic
- Prior art date
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- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0676—Pancreatic cells
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- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
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Abstract
"MéTODOS PARA EXPANSãO E TRANSDIFERENCIAçãO IN VITRO DE CéLULAS ACINARES PANCREáTICAS HUMANAS EM CéLULAS PRODUTORAS DE INSULINA". Esta invenção diz respeito, por exemplo, a um método para expandir células acinares de mamífero, que compreende cultivar as células em um sistema de cultura de células que compreende um meio de cultura de células e uma superfície de anexação das células, sob condições em que as células acinares são submetidas a uma expansão de 3-4 vezes juntamente com transdiferenciação em um fenótipo de célula modificada (células IP) que apresenta características de células acinares e células hepáticas. A invenção também diz respeito a um método para transformar essas células IP em células produtoras de insulina in vitro, que compreende cultivar as células em um meio inédito definido. São também revelados meios de cultura adequados para realizar esses métodos, células isoladas que têm o fenótipo de células IP e/ou produzidas por esses métodos, e estojos para realizar os métodos.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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US38400002P | 2002-05-28 | 2002-05-28 | |
PCT/US2003/016124 WO2003102134A2 (en) | 2002-05-28 | 2003-05-22 | Pancreatic acinar cells into insulin producing cells |
Publications (1)
Publication Number | Publication Date |
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BR0311360A true BR0311360A (pt) | 2006-06-06 |
Family
ID=29584613
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
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BR0311413A BR0311413A (pt) | 2002-05-28 | 2003-05-22 | Desenvolvimento e transdiferenciação de células acinares humanas |
BR0311360A BR0311360A (pt) | 2002-05-28 | 2003-05-22 | métodos para expansão e transdiferenciação in vitro de células acinares pancreáticas humanas em células produtoras de insulina |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR0311413A BR0311413A (pt) | 2002-05-28 | 2003-05-22 | Desenvolvimento e transdiferenciação de células acinares humanas |
Country Status (8)
Country | Link |
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US (5) | US20060122104A1 (pt) |
EP (3) | EP1578925A4 (pt) |
JP (2) | JP2006512046A (pt) |
CN (2) | CN1662643A (pt) |
AU (3) | AU2003273573A1 (pt) |
BR (2) | BR0311413A (pt) |
CA (2) | CA2487094A1 (pt) |
WO (3) | WO2003102134A2 (pt) |
Families Citing this family (108)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101089591B1 (ko) * | 2001-12-07 | 2011-12-05 | 제론 코포레이션 | 인간 배아 줄기세포 유래의 섬세포 |
US20060122104A1 (en) * | 2002-05-28 | 2006-06-08 | Presnell Sharon C | Methods for in vitro expansion and transdifferentiation of human pancreatic acinar cells into insulin-producing cells |
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