DK163582A - PROCEDURE FOR CLEANING A PHYSIOLOGICALLY ACTIVE SUBSTANCE WITH ANTITUMUM EFFECT - Google Patents

PROCEDURE FOR CLEANING A PHYSIOLOGICALLY ACTIVE SUBSTANCE WITH ANTITUMUM EFFECT Download PDF

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Publication number
DK163582A
DK163582A DK163582A DK163582A DK163582A DK 163582 A DK163582 A DK 163582A DK 163582 A DK163582 A DK 163582A DK 163582 A DK163582 A DK 163582A DK 163582 A DK163582 A DK 163582A
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DK
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Prior art keywords
physiologically active
active substance
substance
gel filtration
gel
Prior art date
Application number
DK163582A
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Danish (da)
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DK158230B (en
DK158230C (en
Inventor
Katsuyuki Haranaka
Original Assignee
Asahi Chemical Ind
Dainippon Pharmaceuical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Asahi Chemical Ind, Dainippon Pharmaceuical Co Ltd filed Critical Asahi Chemical Ind
Priority to DK163582A priority Critical patent/DK158230C/en
Publication of DK163582A publication Critical patent/DK163582A/en
Publication of DK158230B publication Critical patent/DK158230B/en
Application granted granted Critical
Publication of DK158230C publication Critical patent/DK158230C/en

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  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

A protein-like, physiologically active substance having an antineoplastic effect, which substance is induced by administering at least substance which can stimulate the reticuloendothelial system to a rabbit and then injecting the rabbit with endotoxin from a Gram-negative bacterium, is purified. The process comprises bringing a crude solution of the protein-like, physiologically active substance into contact with a basic ion exchanger, with the physiologically active substance being adsorbed with the ion exchanger, eluting the adsorbed physiologically active substance and subjecting the eluate, which contains the physiologically active substance, to gel filtration using a gel which is suitable for separating a substance having a molecular weight in the range of 30,000-70,000. The resulting purified preparation of the physiologically active substance can be used as an antineoplastic agent for treating malignant tumours. The physiologically active substance is isolated by subjecting the purified preparation to affinity chromatography on immobilized Cibacron Blue F3G-A, gel filtration, affinity chromatography and immobilized concanavalin A, preparative polyacrylamide gel electrophoresis and gel filtration, in the given order.
DK163582A 1982-04-07 1982-04-07 PROCEDURE FOR CLEANING A PROTEIN-LIKING PHYSIOLOGICAL ACTIVE SUBSTANCE WITH ANTITUMUM EFFECT AND THE PROTEIN-LIKING PHYSIOLOGICAL ACTIVE SUBSTANCE PURIFIED BY THIS PROCEDURE DK158230C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DK163582A DK158230C (en) 1982-04-07 1982-04-07 PROCEDURE FOR CLEANING A PROTEIN-LIKING PHYSIOLOGICAL ACTIVE SUBSTANCE WITH ANTITUMUM EFFECT AND THE PROTEIN-LIKING PHYSIOLOGICAL ACTIVE SUBSTANCE PURIFIED BY THIS PROCEDURE

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK163582 1982-04-07
DK163582A DK158230C (en) 1982-04-07 1982-04-07 PROCEDURE FOR CLEANING A PROTEIN-LIKING PHYSIOLOGICAL ACTIVE SUBSTANCE WITH ANTITUMUM EFFECT AND THE PROTEIN-LIKING PHYSIOLOGICAL ACTIVE SUBSTANCE PURIFIED BY THIS PROCEDURE

Publications (3)

Publication Number Publication Date
DK163582A true DK163582A (en) 1983-10-08
DK158230B DK158230B (en) 1990-04-16
DK158230C DK158230C (en) 1990-09-17

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Family Applications (1)

Application Number Title Priority Date Filing Date
DK163582A DK158230C (en) 1982-04-07 1982-04-07 PROCEDURE FOR CLEANING A PROTEIN-LIKING PHYSIOLOGICAL ACTIVE SUBSTANCE WITH ANTITUMUM EFFECT AND THE PROTEIN-LIKING PHYSIOLOGICAL ACTIVE SUBSTANCE PURIFIED BY THIS PROCEDURE

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DK (1) DK158230C (en)

Also Published As

Publication number Publication date
DK158230B (en) 1990-04-16
DK158230C (en) 1990-09-17

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