KR830009779A - Purification method of anti-tumor physiologically active substance - Google Patents

Purification method of anti-tumor physiologically active substance Download PDF

Info

Publication number
KR830009779A
KR830009779A KR1019820001464A KR820001464A KR830009779A KR 830009779 A KR830009779 A KR 830009779A KR 1019820001464 A KR1019820001464 A KR 1019820001464A KR 820001464 A KR820001464 A KR 820001464A KR 830009779 A KR830009779 A KR 830009779A
Authority
KR
South Korea
Prior art keywords
active substance
physiologically active
tumor
buffer
purification method
Prior art date
Application number
KR1019820001464A
Other languages
Korean (ko)
Other versions
KR890004692B1 (en
Inventor
가쯔유끼 하라나까
Original Assignee
세꼬 마오미
아사히 가세이 고오교 가부시끼 가이샤
후지와라 도미오
다이닛뽕 세이야꾸 가부시끼 가이샤
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 세꼬 마오미, 아사히 가세이 고오교 가부시끼 가이샤, 후지와라 도미오, 다이닛뽕 세이야꾸 가부시끼 가이샤 filed Critical 세꼬 마오미
Priority to KR8201464A priority Critical patent/KR890004692B1/en
Publication of KR830009779A publication Critical patent/KR830009779A/en
Application granted granted Critical
Publication of KR890004692B1 publication Critical patent/KR890004692B1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/18—Growth factors; Growth regulators

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

내용 없음No content

Description

항종양 생리학적 활성물질의 정제방법Purification method of anti-tumor physiologically active substance

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도 : 생리학적 활성물질을 컬럼 크로마토그라피(1단계) 및 겔여과(2단계)시킨 것이다.FIG. 1: Physiologically active substance is column chromatography (stage 1) and gel filtration (stage 2).

제2도 : 생리학적 활성물질을 컬럼 크로마토그라피(1단계) 및 겔여과(2단계)시킨 것이다.Figure 2: The physiologically active substance is column chromatography (stage 1) and gel filtration (stage 2).

Claims (4)

토끼에 세망내피계 자극작용을 지닌 물질 적어도 1가지를 투여한 후 그탐 음성균으로부터 얻은 엔도톡신을 주사하여 유도된 항종양작용을 지닌 단백질성 생리학적 활성물질의 조용액을 염기성 음이온 교환기와 접촉시켜 음이온 교환기에 생리학적 활성물질을 흡착시키고, 흡착된 생리학적 활성물질을 용출하여, 이 생리학적 활성물질을 함유하는 용출물을 분자량 30,000 내지 70,000 범위의 물질을 분리시키기에 적합한 겔을 사용하여 겔 여과시킴을 특징으로 하여 항종양 작용을 지닌 단백질성 생리학적 활성물질을 정제하는 방법.Anion exchanger was prepared by contacting a basic anion exchanger with a crude solution of an anti-tumor proteinaceous physiologically active substance induced by injecting an endotoxin obtained from gtam-negative bacteria into a rabbit. The physiologically active substance is adsorbed on the effluent and the physiologically active substance is eluted, and the eluate containing the physiologically active substance is subjected to gel filtration using a gel suitable for separating a substance having a molecular weight ranging from 30,000 to 70,000. Characterized in that the purification of the proteinaceous physiologically active substance having an anti-tumor action. 제1항에 있어서, 겔 여과시 용출제가 pH 6.0 내지 9.0의 완충용액인 방법.The method of claim 1 wherein the eluent in gel filtration is a buffer solution of pH 6.0-9.0. 제1항에 있어서, 조용액을 pH 6.0 내지 9.0의 완충용액 및 0.2M 이하 농도의 염을 사용하여 염기성 이온교환기에 접촉시키는 방법.The method of claim 1, wherein the crude solution is contacted with a basic ion exchanger using a pH 6.0-9.0 buffer and a salt at a concentration of 0.2 M or less. 제2항에 있어서, 조 용액을 pH 6.0 내지 9.0의 완충용액 및 0.2M 이하 농도의 염을 사용하여 염기성 이온교환기에 접촉시키는 방법.The method of claim 2, wherein the crude solution is contacted with a basic ion exchanger using a pH 6.0-9.0 buffer and a salt at a concentration of 0.2 M or less. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR8201464A 1982-04-02 1982-04-02 Process for the purification of physiologically active substance having antitumor activity KR890004692B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR8201464A KR890004692B1 (en) 1982-04-02 1982-04-02 Process for the purification of physiologically active substance having antitumor activity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR8201464A KR890004692B1 (en) 1982-04-02 1982-04-02 Process for the purification of physiologically active substance having antitumor activity

Publications (2)

Publication Number Publication Date
KR830009779A true KR830009779A (en) 1983-12-23
KR890004692B1 KR890004692B1 (en) 1989-11-25

Family

ID=19224343

Family Applications (1)

Application Number Title Priority Date Filing Date
KR8201464A KR890004692B1 (en) 1982-04-02 1982-04-02 Process for the purification of physiologically active substance having antitumor activity

Country Status (1)

Country Link
KR (1) KR890004692B1 (en)

Also Published As

Publication number Publication date
KR890004692B1 (en) 1989-11-25

Similar Documents

Publication Publication Date Title
SE8200715L (en) PYROGEN CONTENT REDUCTION
ES553016A0 (en) DEVICE FOR SPECIFIC ADsorption of Heparin
KR840007438A (en) Method for Purifying Anthracyclinone Glucoside
ES2031101T3 (en) METHOD OF PURIFYING TOXIN CONJUGATES USING HYDROPHOBA INTERACTION CHROMATOGRAPHY.
ES2051684T3 (en) PREPARATION METHOD OF ALPHA-1-PROTEINASE INHIBITOR.
IL79972A0 (en) Protein recovery
EP0239565A3 (en) Method of and apparatus for separating proteins
BE892846A (en) PROCESS FOR THE PURIFICATION OF A PHYSIOLOGICALLY ACTIVE SUBSTANCE WITH ANTI-TUMOR ACTIVITY, AND SUBSTANCE THUS OBTAINED
KR840003422A (en) Method of producing homogeneous human immune interferon
KR870003056A (en) Basic amino acid separation method
Hochwald et al. Trace proteins in cerebrospinal fluid and other biological fluids I. Effect of various fractionation procedures on β-trace and γ-trace proteins and methods for isolation of both proteins
GB1440369A (en) Purification of coenzyme a
KR860009121A (en) Preparation of Treponema Hiodicenteria bacterin
SE8600641D0 (en) NITRILOFORA EDA ABSORBENTS
KR830009779A (en) Purification method of anti-tumor physiologically active substance
KR910009283A (en) Process for Purifying Pertussis Toxin and / or Sasang Hemagglutinin Antigen
KR850001285A (en) Manufacturing method of natural human interleukin-2
Carroll et al. The mechanism of calcium and magnesium uptake from sea water by a marine bacterium
ES2041690T3 (en) METHOD FOR THE PURIFICATION OF ANTIBODIES.
ES456196A1 (en) Method for the purification of heparin
JPS57140726A (en) Purification of physiologically active substance having carcinostatic action
KR850001688A (en) Therapeutic composition using liposome containing fructose 1-6 diphosphate and method for preparing same
KR860000868A (en) LPF-HA Purification Method
KR860009034A (en) Treatment method of nucleic acid purified mother liquor
Porterfield The effect of calcium and electrolytes on the enzymic action of influenza viruses and v. cholerae extract

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E601 Decision to refuse application
J2X1 Appeal (before the patent court)

Free format text: TRIAL NUMBER: 1988201001082; APPEAL AGAINST DECISION TO DECLINE REFUSAL

G160 Decision to publish patent application
O035 Opposition [patent]: request for opposition
O073 Decision to grant registration after opposition [patent]: decision to grant registration
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20001115

Year of fee payment: 12

LAPS Lapse due to unpaid annual fee