ES8304150A1 - A procedure for purifying a physiologically active protein substance that has antitumorigenic activity - Google Patents

A procedure for purifying a physiologically active protein substance that has antitumorigenic activity

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Publication number
ES8304150A1
ES8304150A1 ES511376A ES511376A ES8304150A1 ES 8304150 A1 ES8304150 A1 ES 8304150A1 ES 511376 A ES511376 A ES 511376A ES 511376 A ES511376 A ES 511376A ES 8304150 A1 ES8304150 A1 ES 8304150A1
Authority
ES
Spain
Prior art keywords
physiologically active
substance
active substance
procedure
active protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES511376A
Other languages
Spanish (es)
Other versions
ES511376A0 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Asahi Kasei Corp
Dainippon Pharmaceutical Co Ltd
Asahi Chemical Industry Co Ltd
Original Assignee
Dainippon Pharmaceutical Co Ltd
Asahi Chemical Industry Co Ltd
Asahi Kasei Kogyo KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dainippon Pharmaceutical Co Ltd, Asahi Chemical Industry Co Ltd, Asahi Kasei Kogyo KK filed Critical Dainippon Pharmaceutical Co Ltd
Priority to ES511376A priority Critical patent/ES8304150A1/en
Publication of ES511376A0 publication Critical patent/ES511376A0/en
Publication of ES8304150A1 publication Critical patent/ES8304150A1/en
Expired legal-status Critical Current

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Abstract

A procedure is provided for the purification of a physiologically active protein substance that has antitumorigenic activity, which is induced by the administration to a rabbit of at least one substance which is able to stimulate the reticuloendothelial system and, afterwards, by injection into the rabbit of endotoxin from a Gram-negative bacterium. The procedure involves bringing a raw solution of said physiologically active protein substance into contact with a basic anion exchanger, in order to adsorb said physiologically active substance on the anion exchanger, elute the physiologically active substance adsorbed, and subject the eluate which contains said physiologically active substance to gel filtration, with a gel that is suitable for the separation of a substance with a molecular weight in the range from 30,000 to 70,000. The purified preparation of said physiologically active substance so obtained can be used as an antitumorigenic agent for the treatment of malignant tumours. Said physiologically active substance is isolated by subjecting the purified preparation successively to affinity chromatography on immobilized Cibacron Blue F3G-A, gel filtration, affinity chromatography on immobilized concanavalin A, preparative polyacrylamide gel electrophoresis, and gel filtration.
ES511376A 1982-04-13 1982-04-13 A procedure for purifying a physiologically active protein substance that has antitumorigenic activity Expired ES8304150A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES511376A ES8304150A1 (en) 1982-04-13 1982-04-13 A procedure for purifying a physiologically active protein substance that has antitumorigenic activity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES511376A ES8304150A1 (en) 1982-04-13 1982-04-13 A procedure for purifying a physiologically active protein substance that has antitumorigenic activity

Publications (2)

Publication Number Publication Date
ES511376A0 ES511376A0 (en) 1983-02-16
ES8304150A1 true ES8304150A1 (en) 1983-02-16

Family

ID=8483993

Family Applications (1)

Application Number Title Priority Date Filing Date
ES511376A Expired ES8304150A1 (en) 1982-04-13 1982-04-13 A procedure for purifying a physiologically active protein substance that has antitumorigenic activity

Country Status (1)

Country Link
ES (1) ES8304150A1 (en)

Also Published As

Publication number Publication date
ES511376A0 (en) 1983-02-16

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