DK0640141T3 - Nucleinsyre-fragmenter og fremgangsmåde til forøgelse af lysin og threonin indholdet i plantefrø - Google Patents

Nucleinsyre-fragmenter og fremgangsmåde til forøgelse af lysin og threonin indholdet i plantefrø

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Publication number
DK0640141T3
DK0640141T3 DK93908395T DK93908395T DK0640141T3 DK 0640141 T3 DK0640141 T3 DK 0640141T3 DK 93908395 T DK93908395 T DK 93908395T DK 93908395 T DK93908395 T DK 93908395T DK 0640141 T3 DK0640141 T3 DK 0640141T3
Authority
DK
Denmark
Prior art keywords
nucleic acid
acid fragments
plant seeds
threonine content
increasing lysine
Prior art date
Application number
DK93908395T
Other languages
English (en)
Inventor
Saverio Carl Falco
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Application granted granted Critical
Publication of DK0640141T3 publication Critical patent/DK0640141T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/04Alpha- or beta- amino acids
    • C12P13/08Lysine; Diaminopimelic acid; Threonine; Valine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254Tryptophan or lysine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0012Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0012Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0012Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1217Phosphotransferases with a carboxyl group as acceptor (2.7.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK93908395T 1992-03-19 1993-03-18 Nucleinsyre-fragmenter og fremgangsmåde til forøgelse af lysin og threonin indholdet i plantefrø DK0640141T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US85541492A 1992-03-19 1992-03-19
PCT/US1993/002480 WO1993019190A1 (en) 1992-03-19 1993-03-18 Nucleic acid fragments and methods for increasing the lysine and threonine content of the seeds of plants

Publications (1)

Publication Number Publication Date
DK0640141T3 true DK0640141T3 (da) 2003-07-21

Family

ID=25321215

Family Applications (2)

Application Number Title Priority Date Filing Date
DK03009812T DK1394257T3 (da) 1992-03-19 1993-03-18 Nukleinsyrefragmenter og fremgangsmåder til forögelse af lysin- og threonin-indholdet i fröene fra planter
DK93908395T DK0640141T3 (da) 1992-03-19 1993-03-18 Nucleinsyre-fragmenter og fremgangsmåde til forøgelse af lysin og threonin indholdet i plantefrø

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK03009812T DK1394257T3 (da) 1992-03-19 1993-03-18 Nukleinsyrefragmenter og fremgangsmåder til forögelse af lysin- og threonin-indholdet i fröene fra planter

Country Status (9)

Country Link
EP (2) EP1394257B1 (da)
JP (1) JPH07504821A (da)
AU (1) AU675933B2 (da)
CA (1) CA2132414C (da)
DE (2) DE69334163T2 (da)
DK (2) DK1394257T3 (da)
MX (1) MX9301521A (da)
WO (1) WO1993019190A1 (da)
ZA (1) ZA931978B (da)

Families Citing this family (38)

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US7705215B1 (en) * 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5550318A (en) * 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
ATE217319T1 (de) * 1992-10-05 2002-05-15 Univ North Carolina State Verfahren zur erhöhung der expression und zur verminderung der expressionsvariabilität von fremdgenen in pflanzenzellen
EP0643135B1 (en) * 1992-11-10 2001-08-01 Ajinomoto Co., Inc. Dna encoding aspartokinase iii mutants and their use for the production of l-threonine by fermentation
EP0745126B1 (en) * 1993-01-13 2001-09-12 Pioneer Hi-Bred International, Inc. High lysine derivatives of alpha-hordothionin
US6118047A (en) 1993-08-25 2000-09-12 Dekalb Genetic Corporation Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction
HU222085B1 (hu) * 1993-11-30 2003-04-28 E.I. Du Pont De Nemours And Co. Kiméragének és eljárások kukorica-, szójabab- és repcemagvak lizintartalmának növelésére
JPH07155184A (ja) * 1993-12-08 1995-06-20 Ajinomoto Co Inc 発酵法によるl−リジンの製造法
IL113685A0 (en) * 1994-05-13 1995-08-31 Du Pont Nucleic acid fragments chimeric genes and methods for increasing the methionine content of the seeds of plants
ZA955629B (en) * 1994-07-08 1997-01-06 Du Pont Chimeric genes and method for increasing the threonine content of the seeds of plants
AU5956196A (en) * 1995-05-31 1996-12-18 Pioneer Hi-Bred International, Inc. Methods of increasing accumulation of essential amino acids n seeds
WO1996038563A1 (en) * 1995-06-02 1996-12-05 Pioneer Hi-Bred International, Inc. HIGH METHIONINE DERIVATIVES OF α-HORDOTHIONIN
BR9609200A (pt) * 1995-06-02 1999-05-11 Pioneer Hi Bred Int Derivados de alta treonina de alpha-hordotionina
EP0834559B1 (en) * 1995-06-13 2003-07-30 Ajinomoto Co., Inc. Process for producing l-lysine by fermentation
US6166292A (en) * 1996-04-26 2000-12-26 Ajinomoto Co., Inc. Raffinose synthetase gene, method of producing raffinose and transgenic plant
US6891084B1 (en) 1996-04-26 2005-05-10 Ajinomoto Co., Inc. DNA encoding raffinose synthase from soybean
JP4088982B2 (ja) * 1996-10-15 2008-05-21 味の素株式会社 発酵法によるl−アミノ酸の製造法
AU7509096A (en) * 1996-11-18 1998-06-10 Avebe Transgenic plant or plants with a naturally high water content overproducing at least two amino acids of the aspartate family
BR9811256A (pt) * 1997-03-27 2000-07-18 Du Pont Fragmento de ácido nucleico, gene quimérico, célula vegetal, semente de planta, método para reduzir a atividade da lisina cetoglutarato redutase em uma semente de planta e planta
AU8148398A (en) 1997-07-15 1999-02-10 Dow Agrosciences Llc Nucleotide sequences of genes encoding sink proteins and uses thereof for improving the nutritional quality of feeds
EP1440151B1 (en) * 2001-09-05 2008-02-27 Monsanto Technology LLC Seed specific 7s alpha promoter for expressing genes in plants
US8008545B2 (en) 2003-04-15 2011-08-30 Basf Plant Science Gmbh Process for the production of fine chemicals
EP1654368A2 (en) 2003-08-01 2006-05-10 BASF Plant Science GmbH Process for the production of fine chemicals
US7157281B2 (en) * 2003-12-11 2007-01-02 Monsanto Technology Llc High lysine maize compositions and event LY038 maize plants
US7855323B2 (en) 2004-02-10 2010-12-21 Monsanto Technology Llc Recombinant DNA for gene suppression
CN1954076B (zh) 2004-04-06 2013-11-27 梅坦诺米克斯有限公司 产生精细化学品的方法
EP2080769A3 (en) 2004-07-02 2010-12-01 Metanomics GmbH Process for the production of fine chemicals
MX2007007040A (es) 2004-12-17 2008-10-24 Metanomics Gmbh Proceso para el control de produccion de productos quimicos finos.
CA2598792A1 (en) 2005-03-02 2006-09-08 Metanomics Gmbh Process for the production of fine chemicals
US20140199313A1 (en) 2005-03-02 2014-07-17 Metanomics Gmbh Process for the Production of Fine Chemicals
EP1931790B1 (en) * 2005-10-03 2012-01-25 Monsanto Technology, LLC Transgenic plant seed with increased lysine
CA2644273A1 (en) 2006-04-05 2008-03-27 Metanomics Gmbh Process for the production of a fine chemical
BRPI0810083B8 (pt) 2007-03-28 2020-10-06 Monsanto Technology Llc métodos para estabelecer onde uma planta de soja ou semente de soja deve ser cultivada, de melhoramento da planta de soja, para selecionar uma semente de soja, de distribuição de uma planta de soja, para isolar sementes de soja de maturidade precoce indeterminada, e para determinar se uma planta de soja tem um grupo de maturidade
CA2704725C (en) 2007-09-11 2018-01-02 Monsanto Technology Llc Increased alpha-prime beta-conglycinin soybeans
WO2010002876A2 (en) * 2008-06-30 2010-01-07 Monsanto Technology Llc Compositions and methods for enhanced amino acid levels in plants
CN108486082A (zh) 2012-10-18 2018-09-04 中粮生物化学(安徽)股份有限公司 天冬氨酸激酶iii突变体及其宿主细胞和应用
CN106086011B (zh) * 2016-06-18 2019-10-18 北京大北农科技集团股份有限公司 用于检测除草剂耐受性大豆植物dbn9004的核酸序列及其检测方法
CN113201514B (zh) 2020-10-16 2022-09-06 中国科学院天津工业生物技术研究所 具有天冬氨酸激酶活性的多肽及其在生产氨基酸中的应用

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Publication number Priority date Publication date Assignee Title
US5258300A (en) * 1988-06-09 1993-11-02 Molecular Genetics Research And Development Limited Partnership Method of inducing lysine overproduction in plants
DE3943117A1 (de) * 1989-12-27 1991-07-04 Forschungszentrum Juelich Gmbh Verfahren zur fermentativen herstellung von aminosaeure, insbesondere l-lysin, dafuer geeignete mikroorganismen und rekombinante dna
US5367110A (en) * 1990-11-13 1994-11-22 Yeda Research And Development Co. Ltd. Transgenic plants overproducing threonine and lysine
US7746705B2 (en) * 2007-12-10 2010-06-29 Spansion Llc Selective application of word line bias to minimize fringe effects in electromagnetic fields during erase of nonvolatile memory

Also Published As

Publication number Publication date
MX9301521A (es) 1993-09-01
WO1993019190A1 (en) 1993-09-30
EP0640141A1 (en) 1995-03-01
DK1394257T3 (da) 2007-12-10
AU675933B2 (en) 1997-02-27
ZA931978B (en) 1994-09-19
CA2132414A1 (en) 1993-09-30
EP1394257A1 (en) 2004-03-03
DE69332974D1 (de) 2003-06-18
DE69334163D1 (de) 2007-09-20
EP0640141B1 (en) 2003-05-14
CA2132414C (en) 2009-01-27
EP1394257B1 (en) 2007-08-08
DE69334163T2 (de) 2008-04-30
AU3923393A (en) 1993-10-21
JPH07504821A (ja) 1995-06-01
DE69332974T2 (de) 2004-05-19

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