ATE217319T1 - Verfahren zur erhöhung der expression und zur verminderung der expressionsvariabilität von fremdgenen in pflanzenzellen - Google Patents

Verfahren zur erhöhung der expression und zur verminderung der expressionsvariabilität von fremdgenen in pflanzenzellen

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Publication number
ATE217319T1
ATE217319T1 AT93922757T AT93922757T ATE217319T1 AT E217319 T1 ATE217319 T1 AT E217319T1 AT 93922757 T AT93922757 T AT 93922757T AT 93922757 T AT93922757 T AT 93922757T AT E217319 T1 ATE217319 T1 AT E217319T1
Authority
AT
Austria
Prior art keywords
expression
plant cells
foreign genes
transcription initiation
initiation region
Prior art date
Application number
AT93922757T
Other languages
English (en)
Inventor
William F Thomson
Steven L Spiker
George C Allen
Gerald E Hall Jr
Lisa C Childs
Original Assignee
Univ North Carolina State
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ North Carolina State filed Critical Univ North Carolina State
Application granted granted Critical
Publication of ATE217319T1 publication Critical patent/ATE217319T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/822—Reducing position variability, e.g. by the use of scaffold attachment region/matrix attachment region (SAR/MAR); Use of SAR/MAR to regulate gene expression

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Fruits And Vegetables (AREA)
AT93922757T 1992-10-05 1993-09-27 Verfahren zur erhöhung der expression und zur verminderung der expressionsvariabilität von fremdgenen in pflanzenzellen ATE217319T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US95642092A 1992-10-05 1992-10-05
PCT/US1993/009179 WO1994007902A1 (en) 1992-10-05 1993-09-27 Method for increasing expression and reducing expression variability of foreign genes in plant cells

Publications (1)

Publication Number Publication Date
ATE217319T1 true ATE217319T1 (de) 2002-05-15

Family

ID=25498228

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93922757T ATE217319T1 (de) 1992-10-05 1993-09-27 Verfahren zur erhöhung der expression und zur verminderung der expressionsvariabilität von fremdgenen in pflanzenzellen

Country Status (10)

Country Link
US (1) US5773689A (de)
EP (1) EP0663921B1 (de)
AT (1) ATE217319T1 (de)
AU (1) AU673859B2 (de)
CA (1) CA2147006C (de)
DE (1) DE69331908T2 (de)
DK (1) DK0663921T3 (de)
ES (1) ES2176208T3 (de)
PT (1) PT663921E (de)
WO (1) WO1994007902A1 (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6239328B1 (en) 1992-10-05 2001-05-29 North Carolina State University Method for reducing expression variability of transgenes in plant cells
AU6679094A (en) * 1993-04-19 1994-11-08 Sandoz Ltd. Genetic stabilizing elements
US6040185A (en) * 1993-04-19 2000-03-21 Novartis Ag Genetic stabilizing elements
US5773695A (en) * 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
US6037525A (en) * 1996-08-01 2000-03-14 North Carolina State University Method for reducing expression variability of transgenes in plant cells
IL129268A0 (en) * 1996-10-17 2000-02-17 Du Pont Enhanced transgene expression in a population of monocot cells employing scaffold attachment regions
AU6871698A (en) * 1997-03-28 1998-10-22 Mycogen Plant Science, Inc. Improved sar plant transformation process
CA2292842A1 (en) * 1997-06-03 1998-12-10 North Carolina State University Method for reducing expression variability of transgenes in plants cells
US6245974B1 (en) * 1997-08-06 2001-06-12 North Carolina State University Matrix attachment regions
US6518485B1 (en) * 1998-06-04 2003-02-11 Westvaco Corporation Particle-mediated conifer transformation
AU3476399A (en) * 1998-07-31 2000-02-21 Mycogen Plant Science, Inc. Improved plant transformation process by scaffold attachment regions (sar)
AU762382B2 (en) * 1998-09-29 2003-06-26 Pioneer Hi-Bred International, Inc. MAR/SAR elements flanking RSYN7-driven construct
US6482646B1 (en) 1998-11-06 2002-11-19 E. I. Du Pont De Nemours And Company Plant proteins that interact with nuclear matrix proteins and function as transcriptional activators
ATE279524T1 (de) * 1998-12-01 2004-10-15 Dow Agrosciences Llc Künstliche matrix-anheftungsregion zur erhöhung der expression von in pflanzen eingeführten genen
US6395962B1 (en) 1999-06-22 2002-05-28 University Of South Carolina Enhancing expression of a silenced target sequence in plants using plant viral enhancers and amplicons
US7126041B1 (en) 1999-12-10 2006-10-24 North Carolina State Unversity High efficiency gene targeting in plants
KR100408844B1 (ko) 2000-07-29 2003-12-06 한국산업기술평가원 동물세포 발현벡터
WO2002061039A2 (en) * 2000-10-25 2002-08-08 Washington State University Research Foundation Thuja plicata dirigent protein promotors
CA2435972C (en) 2001-01-26 2011-09-13 University Of Lausanne Matrix attachment regions and methods for use thereof
DE10114209A1 (de) * 2001-03-23 2002-12-05 Icon Genetics Ag Ortsgerichtete Transformation durch Verwendung von Amplifikationsvektoren
EP1279737A1 (de) 2001-07-27 2003-01-29 Coöperatieve Verkoop- en Productievereniging, van Aardappelmeel en Derivaten AVEBE B.A. Transformationsmethode zur Erzeugung von Pflanzen ohne Selektionsmarker
US20040016014A1 (en) * 2002-07-17 2004-01-22 Nguyen Thanh-Tuyen T. High efficiency germline transformation system
US20040016015A1 (en) * 2002-07-17 2004-01-22 Nguyen Thanh-Tuyen T. High efficiency germline transformation system
JP2007502621A (ja) * 2003-08-19 2007-02-15 ２２ンド センチュリー リミテッド，エルエルシー 曝露低減タバコ製品
KR101229989B1 (ko) 2003-10-24 2013-02-06 셀렉시스 에스. 에이. Ｍａｒ 서열의 복합 트랜스펙션 방법에 의한 포유동물 세포에서의 고효율 유전자 전달 및 발현
EP1942931A4 (de) 2005-11-04 2009-04-29 Dow Agrosciences Llc Herstellung von vakzin-master-zelllinien mit rekombinanten pflanzensuspensionskulturen
AR064500A1 (es) 2006-12-22 2009-04-08 Dow Agrosciences Llc Vacunas del virus west nile (wnv) de plantas, vectores y secuencias optimizadas de codones de plantas
DK2655629T3 (da) * 2010-12-24 2024-07-15 BASF Agricultural Solutions Seed US LLC Brassica-plante, som omfatter en mutant alcatraz-allel
EP2761003A4 (de) * 2011-09-15 2015-11-11 Basf Plant Science Co Gmbh Regulatorische nukleinsäuremoleküle für zuverlässige genexpression bei pflanzen
EP3782465B1 (de) 2019-08-23 2023-08-30 Universität Potsdam Transgenes zebrafischmodell für schlaganfall

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5122466A (en) * 1989-06-13 1992-06-16 North Carolina State University Ballistic transformation of conifers
US5550318A (en) * 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
EP0519011A1 (de) * 1990-03-05 1992-12-23 The Upjohn Company Protein-expression durch verwendung von samenspezifischen regulierungssequenzen
US5187267A (en) * 1990-06-19 1993-02-16 Calgene, Inc. Plant proteins, promoters, coding sequences and use
MX9200621A (es) * 1991-02-14 1993-02-01 Du Pont Gen de una proteina con alto contenido de azufre de una semilla y metodo para aumentar el contenido de azufre en aminoacidos de las plantas.
JPH07504821A (ja) * 1992-03-19 1995-06-01 イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー 植物の種子のリシンおよびスレオニン含有量を増加させるための核酸断片および方法
AU6679094A (en) * 1993-04-19 1994-11-08 Sandoz Ltd. Genetic stabilizing elements

Also Published As

Publication number Publication date
US5773689A (en) 1998-06-30
DE69331908D1 (de) 2002-06-13
WO1994007902A1 (en) 1994-04-14
CA2147006A1 (en) 1994-04-14
AU673859B2 (en) 1996-11-28
CA2147006C (en) 2004-11-30
ES2176208T3 (es) 2002-12-01
PT663921E (pt) 2002-10-31
DK0663921T3 (da) 2002-09-02
DE69331908T2 (de) 2002-12-12
EP0663921A1 (de) 1995-07-26
EP0663921A4 (de) 1996-07-17
AU5165593A (en) 1994-04-26
EP0663921B1 (de) 2002-05-08

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Legal Events

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UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee