DE69920749D1 - Rekombinante bakterienstämme zur herstellung von natürlichen nucleosiden, sowie von modifizierten analogen davon - Google Patents

Rekombinante bakterienstämme zur herstellung von natürlichen nucleosiden, sowie von modifizierten analogen davon

Info

Publication number
DE69920749D1
DE69920749D1 DE69920749T DE69920749T DE69920749D1 DE 69920749 D1 DE69920749 D1 DE 69920749D1 DE 69920749 T DE69920749 T DE 69920749T DE 69920749 T DE69920749 T DE 69920749T DE 69920749 D1 DE69920749 D1 DE 69920749D1
Authority
DE
Germany
Prior art keywords
stranks
production
recombinant bacteria
natural nucleosides
modified analogs
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69920749T
Other languages
English (en)
Other versions
DE69920749T2 (de
Inventor
Giuseppina Bestetti
Simona Cali
Daniela Ghisotti
Gaetano Orsini
Giancarlo Tonon
Gabriele Zuffi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SICOR Societa Italiana Corticosteroidi SRL
Original Assignee
KERYOS SpA MILANO
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by KERYOS SpA MILANO filed Critical KERYOS SpA MILANO
Application granted granted Critical
Publication of DE69920749D1 publication Critical patent/DE69920749D1/de
Publication of DE69920749T2 publication Critical patent/DE69920749T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/38—Nucleosides
    • C12P19/40—Nucleosides having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same ring, e.g. purine nucleosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/38—Nucleosides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69920749T 1998-12-23 1999-12-23 Rekombinante bakterienstämme zur herstellung von natürlichen nucleosiden, sowie von modifizierten analogen davon Expired - Lifetime DE69920749T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
ITMI982792 1998-12-23
IT1998MI002792A IT1304500B1 (it) 1998-12-23 1998-12-23 Ceppi batterici ricombinati per la produzione di nucleosidi naturali edi analoghi modificati.
PCT/EP1999/010416 WO2000039307A2 (en) 1998-12-23 1999-12-23 Recombinant bacterial strains for the production of natural nucleosides and modified analogues thereof

Publications (2)

Publication Number Publication Date
DE69920749D1 true DE69920749D1 (de) 2004-11-04
DE69920749T2 DE69920749T2 (de) 2006-03-02

Family

ID=11381317

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69920749T Expired - Lifetime DE69920749T2 (de) 1998-12-23 1999-12-23 Rekombinante bakterienstämme zur herstellung von natürlichen nucleosiden, sowie von modifizierten analogen davon

Country Status (9)

Country Link
US (2) US6911341B2 (de)
EP (1) EP1141328B1 (de)
JP (1) JP2002533126A (de)
AT (1) ATE278021T1 (de)
AU (1) AU2103000A (de)
DE (1) DE69920749T2 (de)
ES (1) ES2229807T3 (de)
IT (1) IT1304500B1 (de)
WO (1) WO2000039307A2 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1304500B1 (it) * 1998-12-23 2001-03-19 Norpharma Spa Ceppi batterici ricombinati per la produzione di nucleosidi naturali edi analoghi modificati.
US7037718B2 (en) * 2001-10-26 2006-05-02 Cornell Research Foundation, Inc. Mutant purine nucleoside phosphorylase proteins and cellular delivery thereof
US7488598B2 (en) 2001-10-26 2009-02-10 Cornell Center For Technology Enterprise And Commercialization Mutant purine nucleoside phosphorylase proteins and cellular delivery thereof
ES2235638B1 (es) * 2003-12-17 2006-10-16 Cellerix, S.L. Nuevo vector retroviral de alto titulo y metodos de transduccion.
ES2343741T3 (es) * 2006-03-15 2010-08-09 Explora Laboratories Sa Un proceso para la inmovilizacion de celulas en una resina.
EP2338985A1 (de) * 2009-12-22 2011-06-29 Institut Univ. de Ciència i Tecnologia, s.a. Wärmebeständige Biokatalysatorkombination zur Nukleotidsynthese
JP2016198075A (ja) * 2015-04-14 2016-12-01 プラズミア バイオテック，エス．エル． シトシンヌクレオシドアナログの酵素産生
CN113969299B (zh) * 2021-10-17 2023-10-20 拓新药业集团股份有限公司 一种生物转化合成尿苷的方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1573777A (en) * 1977-11-03 1980-08-28 Wellcome Found 9-d-arabinonucleosides and an enzymatic process for their preparation
HU185928B (en) * 1980-04-23 1985-04-28 Wellcome Found Process for preparing 4-substituted-1beta-d-ribofuranosyl-1h-imidazo/4,5-c/pyridines
US4347315A (en) * 1980-04-25 1982-08-31 Burroughs Wellcome Co. Synthesis of ribosides using bacterial phosphorylase
US4835104A (en) 1987-06-16 1989-05-30 Ajinomoto Co., Inc., Patent & Licensing Department Process for producing and purifying 2',3'-dideoxynucleosides, and process for producing 2',3'-dideoxy-2',3'-didehydronucleosides
JP2619710B2 (ja) * 1989-02-27 1997-06-11 日本製紙 株式会社 ２′，３′−ジデオキシプリンヌクレオシド類の製造方法
EP0423641A3 (en) * 1989-10-18 1992-01-15 Nippon Mining Company Limited Expression vector, transformed microorganism, fusion protein and method for the production of platelet factor 4 or tgfalpha
JPH06253854A (ja) * 1993-02-26 1994-09-13 Yamasa Shoyu Co Ltd 組換えｄｎａ手法によるヌクレオシド・ホスホリラーゼの製造法
JP4304727B2 (ja) * 1996-11-21 2009-07-29 味の素株式会社 ヌクレオシド−５’−燐酸エステルの製造法
US6229001B1 (en) * 1997-01-03 2001-05-08 Southern Research Institute Mycobacterium fol a gene that encodes for the enzyme dihydrofolate reductase
JPH10286097A (ja) * 1997-04-15 1998-10-27 Yamasa Shoyu Co Ltd プリンアラビノシドの酵素的製造法
JP3799784B2 (ja) 1997-11-14 2006-07-19 味の素株式会社 ２，６−ジアミノプリン−２’−デオキシリボシドおよび２’−デオキシグアノシンの製造法
IT1304500B1 (it) * 1998-12-23 2001-03-19 Norpharma Spa Ceppi batterici ricombinati per la produzione di nucleosidi naturali edi analoghi modificati.

Also Published As

Publication number Publication date
WO2000039307A3 (en) 2000-11-09
AU2103000A (en) 2000-07-31
US20030059870A1 (en) 2003-03-27
IT1304500B1 (it) 2001-03-19
EP1141328B1 (de) 2004-09-29
JP2002533126A (ja) 2002-10-08
DE69920749T2 (de) 2006-03-02
US7781189B2 (en) 2010-08-24
ATE278021T1 (de) 2004-10-15
US20050142645A1 (en) 2005-06-30
WO2000039307A2 (en) 2000-07-06
US6911341B2 (en) 2005-06-28
ES2229807T3 (es) 2005-04-16
EP1141328A2 (de) 2001-10-10
ITMI982792A1 (it) 2000-06-23

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Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: KERYOS S.P.A., GESSATE, IT

8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: BIO-KER SRL, GESSATE, IT

8327 Change in the person/name/address of the patent owner

Owner name: SICOR SRL, MAILAND, IT

8327 Change in the person/name/address of the patent owner

Owner name: SICOR SOCIETA ITALIANA CORTICOSTEROIDI S.R.L.,, IT