AR001678A1 - Variantes de glucanotransferasa de ciclomaltodextrina construccion de adn vector de expresion recombinante celula huesped método para producir variantes usos de dichas variantes método para modificar la adhesion y/o especialmente de la enzima CGTasa precursora - Google Patents
Variantes de glucanotransferasa de ciclomaltodextrina construccion de adn vector de expresion recombinante celula huesped método para producir variantes usos de dichas variantes método para modificar la adhesion y/o especialmente de la enzima CGTasa precursoraInfo
- Publication number
- AR001678A1 AR001678A1 AR33624396A AR33624396A AR001678A1 AR 001678 A1 AR001678 A1 AR 001678A1 AR 33624396 A AR33624396 A AR 33624396A AR 33624396 A AR33624396 A AR 33624396A AR 001678 A1 AR001678 A1 AR 001678A1
- Authority
- AR
- Argentina
- Prior art keywords
- variants
- cgtase
- producing
- host cell
- expression vector
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
- C12N9/1074—Cyclomaltodextrin glucanotransferase (2.4.1.19)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01019—Cyclomaltodextrin glucanotransferase (2.4.1.19)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La presente invención se refiere a variantes de ciclomaltodextrina glucanotransferasa. Más específicamente la invención se refiere a un métodopara modificar la adhesión del sustrato y/o la especificidad del producto de una enzima CGTasap recursora, y variantes de CGTasa derivadas de una enzimaCGTasa precursora mediante sustitución, inserción y/o deleción de uno o más residuos amino ácidos, cuyos residuos amino ácidos mantinen una posiciónpróxima al sustrato. Además, lainvención se refiere a construcciones ADN que codifican las variantes CGTasa, los vectores de expresión, las células huéspedy los métodos para producir variantes CGTasa de acuerdo con la invención.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK47795 | 1995-04-21 | ||
DK117395 | 1995-10-17 | ||
DK128195 | 1995-11-16 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR001678A1 true AR001678A1 (es) | 1997-11-26 |
Family
ID=27220709
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AR33624396A AR001678A1 (es) | 1995-04-21 | 1996-04-22 | Variantes de glucanotransferasa de ciclomaltodextrina construccion de adn vector de expresion recombinante celula huesped método para producir variantes usos de dichas variantes método para modificar la adhesion y/o especialmente de la enzima CGTasa precursora |
Country Status (11)
Country | Link |
---|---|
US (1) | US6004790A (es) |
EP (2) | EP1632566A3 (es) |
JP (1) | JP4057055B2 (es) |
KR (1) | KR19990007930A (es) |
AR (1) | AR001678A1 (es) |
AT (1) | ATE311439T1 (es) |
AU (1) | AU5396896A (es) |
CA (1) | CA2217876A1 (es) |
DE (1) | DE69635515T2 (es) |
DK (1) | DK0822982T3 (es) |
WO (1) | WO1996033267A1 (es) |
Families Citing this family (43)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19615336A1 (de) * | 1996-04-18 | 1997-10-23 | Consortium Elektrochem Ind | Cyclodextringlycosyltransferasen zur Produktion von gamma-Cyclodextrin |
US6472192B1 (en) | 1996-04-18 | 2002-10-29 | Consortium für elektrochemische Industrie GmbH | Cyclodextrin glycosyl transferases for producing γ-cyclodextrin |
DE19718978A1 (de) * | 1997-05-05 | 1998-11-12 | Henkel Kgaa | Verfahren zur Inhibierung der Farbübertragung bei Textilien während des Waschens |
US6271010B1 (en) * | 1997-09-24 | 2001-08-07 | Novzymes A/S | Cyclomaltodextrin glucanotransferase variants |
DE69931615T2 (de) * | 1998-02-27 | 2007-05-10 | Novozymes A/S | Varianten amylolytischer enzyme |
US6876932B1 (en) | 1998-02-27 | 2005-04-05 | Novozymes A/S | Maltogenic alpha-amylase variants |
AU757935B2 (en) | 1998-02-27 | 2003-03-13 | Novozymes A/S | Maltogenic alpha-amylase variants |
CA2320813A1 (en) | 1998-02-27 | 1999-09-02 | Novo Nordisk A/S | Amylolytic enzyme variants |
WO2001016349A1 (en) * | 1999-09-01 | 2001-03-08 | Novozymes A/S | Method for production of maltose and/or enzymatically modified starch |
JP3934851B2 (ja) * | 2000-05-23 | 2007-06-20 | 日本食品化工株式会社 | 新規シクロテ゛キストリン・ク゛ルカノトランスフェラーセ゛、その製造方法及びこの酵素を用いるシクロテ゛キストリンの製造方法 |
US7700338B2 (en) * | 2000-07-19 | 2010-04-20 | Novozymes A/S | CGTase and DNA sequence encoding same |
DK1337648T3 (da) | 2000-11-28 | 2008-01-07 | Henkel Kgaa | Ny cyclodextrin-glucanotransferase (CGTase) fra Bacillus agaradherens (DSM 9948) samt vaske- og rengöringsmidler med denne nye cyclodextrin-glucanotransferase |
US6960461B2 (en) | 2001-07-11 | 2005-11-01 | Nihon Shokuhin Kako Co., Ltd. | Gene coding for cyclodextrin glucanotransferase chiefly producing γ-cyclodextrin and use thereof |
EP1429626B1 (en) | 2001-09-18 | 2011-03-09 | Novozymes A/S | Enzymatic treatment of starchy food products for shortening the tempering step |
MX264256B (es) * | 2002-02-14 | 2009-02-03 | Novozymes As | Proceso para producir hidrolizado de almidon. |
GB0305685D0 (en) * | 2003-03-12 | 2003-04-16 | Danisco | Enzyme |
US20070148287A1 (en) * | 2003-07-01 | 2007-06-28 | Novozymes A/S | Cgtase variants |
BRPI0511171A (pt) | 2004-05-17 | 2007-12-04 | Procter & Gamble | composição de alvejamento compreendendo um carboidrato oxidase |
CA2575878A1 (en) | 2004-08-02 | 2006-02-09 | Novozymes A/S | Creation of diversity in polypeptides |
JP4989922B2 (ja) * | 2006-06-08 | 2012-08-01 | 日本食品化工株式会社 | 変異体及びこれをコードする遺伝子 |
EP2248907A1 (en) * | 2009-05-08 | 2010-11-10 | Rijksuniversiteit Groningen | Gluco-oligosaccharides comprising (alpha 1-->4) and (alpha 1-->6) glycosidic bonds, use thereof, and methods for providing them |
WO2010138347A1 (en) | 2009-05-26 | 2010-12-02 | The Procter & Gamble Company | Aqueous liquid composition for pre-treating soiled dishware |
WO2011005623A1 (en) | 2009-07-09 | 2011-01-13 | The Procter & Gamble Company | Laundry detergent composition comprising low level of bleach |
US20110009307A1 (en) | 2009-07-09 | 2011-01-13 | Alan Thomas Brooker | Laundry Detergent Composition Comprising Low Level of Sulphate |
CN106220696B (zh) | 2011-03-07 | 2019-12-24 | 株式会社林原 | 含有2-O-α-D-葡萄糖基-L-抗坏血酸无水结晶的粉末的制造方法 |
US8753453B2 (en) | 2012-10-04 | 2014-06-17 | Ecolab Usa Inc. | Pre-soak technology for laundry and other hard surface cleaning |
CN103740669A (zh) * | 2013-04-26 | 2014-04-23 | 江南大学 | 钙离子结合位点氨基酸残基突变改善环糊精葡萄糖基转移酶产β-环糊精能力的方法 |
CN103602641B (zh) * | 2013-11-20 | 2015-05-20 | 广西大学 | 一种产γ-环糊精的β-环糊精葡萄糖基转移酶突变体及其用途 |
CA2956850C (en) | 2014-08-01 | 2021-07-06 | Ecolab Usa Inc. | A method of manual surface cleaning using cleaning textiles and of washing said cleaning textiles |
CN104293743B (zh) * | 2014-09-26 | 2016-09-07 | 江南大学 | 一种受产物抑制减弱的环糊精葡萄糖基转移酶突变体 |
MX2017009108A (es) * | 2015-01-16 | 2017-11-23 | Novozymes As | Metodo para mejorar la capacidad de rebanado de productos horneados. |
US10577571B2 (en) | 2016-11-08 | 2020-03-03 | Ecolab Usa Inc. | Non-aqueous cleaner for vegetable oil soils |
CN111051518B (zh) | 2017-09-04 | 2023-11-03 | 株式会社力森诺科 | 肌醇衍生物的制造方法 |
US20200268780A1 (en) | 2017-09-04 | 2020-08-27 | Showa Denko K.K. | Mixture of inositol derivatives |
CN108034645B (zh) * | 2018-01-15 | 2020-05-08 | 江南大学 | 一种环糊精葡萄糖基转移酶突变体的制备及其应用 |
CN109486786B (zh) * | 2018-12-07 | 2020-09-04 | 江南大学 | 一种环糊精葡萄糖基转移酶突变体 |
US11873465B2 (en) | 2019-08-14 | 2024-01-16 | Ecolab Usa Inc. | Methods of cleaning and soil release of highly oil absorbing substrates employing optimized extended chain nonionic surfactants |
CN113493747A (zh) * | 2020-04-02 | 2021-10-12 | 青岛蔚蓝生物集团有限公司 | 一种高产环糊精葡萄糖基转移酶的突变菌株及其应用 |
CN111534498B (zh) * | 2020-05-28 | 2022-03-25 | 江南大学 | 歧化比活和aa-2g产量提高的环糊精葡萄糖基转移酶突变体 |
WO2022010906A1 (en) | 2020-07-06 | 2022-01-13 | Ecolab Usa Inc. | Peg-modified castor oil based compositions for microemulsifying and removing multiple oily soils |
AU2021306707B2 (en) | 2020-07-06 | 2024-06-13 | Ecolab Usa Inc. | Foaming mixed alcohol/water compositions comprising a structured alkoxylated siloxane |
US20220000757A1 (en) | 2020-07-06 | 2022-01-06 | Ecolab Usa Inc. | Foaming mixed alcohol/water compositions comprising a combination of alkyl siloxane and a hydrotrope/solubilizer |
US20240026248A1 (en) | 2022-07-20 | 2024-01-25 | Ecolab Usa Inc. | Novel nonionic extended surfactants, compositions and methods of use thereof |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5278059A (en) * | 1985-12-04 | 1994-01-11 | Kabushiki Kaisha Hayashibara Seibutsu Kagaki Kenkyujo | Polypeptide possessing cyclomaltodextrin glucanotransferase activity |
WO1989003421A1 (en) * | 1987-10-15 | 1989-04-20 | Novo Industri A/S | Thermostable cyclodextrin glycosyl transferase, its production and use |
DE4009822A1 (de) * | 1990-03-27 | 1991-10-02 | Consortium Elektrochem Ind | (gamma)-cgtase |
US5162210A (en) * | 1990-06-29 | 1992-11-10 | Iowa State University Research Foundation | Process for enzymatic hydrolysis of starch to glucose |
JPH0541985A (ja) * | 1991-03-04 | 1993-02-23 | Oji Koonsutaac Kk | 変異サイクロマルトデキストリングルカノトランスフエラーゼ |
JP3155324B2 (ja) * | 1992-02-04 | 2001-04-09 | 武司 魚住 | サイクロデキストリングルカノトランスフェラーゼ、その製造法、およびその酵素をコードする遺伝子 |
JPH05244945A (ja) * | 1992-03-04 | 1993-09-24 | Nippon Shokuhin Kako Co Ltd | α−サイクロデキストリンの製造方法 |
JP3360291B2 (ja) * | 1993-02-09 | 2002-12-24 | 天野エンザイム株式会社 | γ−サイクロデキストリンの増収方法 |
JPH0740938B2 (ja) * | 1993-03-04 | 1995-05-10 | 工業技術院長 | 変異型の糖質加水分解酵素、該酵素の変異遺伝子及び該酵素を用いたオリゴ糖の製造方法 |
TW383336B (en) * | 1993-06-24 | 2000-03-01 | Consortium Elektrochem Ind | Cyclodextrin glycosyl transferases for the preparation of gama-cyclodextrin |
JPH0723781A (ja) * | 1993-06-25 | 1995-01-27 | Oji Koonsutaac Kk | 改変されたCGTase及びその製造方法並びにその使用方法 |
-
1996
- 1996-04-22 AR AR33624396A patent/AR001678A1/es unknown
- 1996-04-22 KR KR1019970707453A patent/KR19990007930A/ko not_active Application Discontinuation
- 1996-04-22 WO PCT/DK1996/000179 patent/WO1996033267A1/en active IP Right Grant
- 1996-04-22 CA CA002217876A patent/CA2217876A1/en not_active Abandoned
- 1996-04-22 JP JP53141396A patent/JP4057055B2/ja not_active Expired - Fee Related
- 1996-04-22 EP EP05110409A patent/EP1632566A3/en not_active Withdrawn
- 1996-04-22 AT AT96910915T patent/ATE311439T1/de not_active IP Right Cessation
- 1996-04-22 DE DE69635515T patent/DE69635515T2/de not_active Expired - Fee Related
- 1996-04-22 DK DK96910915A patent/DK0822982T3/da active
- 1996-04-22 AU AU53968/96A patent/AU5396896A/en not_active Abandoned
- 1996-04-22 EP EP96910915A patent/EP0822982B1/en not_active Expired - Lifetime
-
1997
- 1997-10-09 US US08/947,965 patent/US6004790A/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
WO1996033267A1 (en) | 1996-10-24 |
ATE311439T1 (de) | 2005-12-15 |
CA2217876A1 (en) | 1996-10-24 |
AU5396896A (en) | 1996-11-07 |
JP4057055B2 (ja) | 2008-03-05 |
US6004790A (en) | 1999-12-21 |
EP0822982B1 (en) | 2005-11-30 |
EP1632566A2 (en) | 2006-03-08 |
DE69635515D1 (de) | 2006-01-05 |
DK0822982T3 (da) | 2006-02-13 |
JPH11503906A (ja) | 1999-04-06 |
KR19990007930A (ko) | 1999-01-25 |
EP0822982A1 (en) | 1998-02-11 |
EP1632566A3 (en) | 2006-08-16 |
DE69635515T2 (de) | 2006-08-10 |
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