IT1304500B1 - Ceppi batterici ricombinati per la produzione di nucleosidi naturali edi analoghi modificati. - Google Patents

Ceppi batterici ricombinati per la produzione di nucleosidi naturali edi analoghi modificati.

Info

Publication number
IT1304500B1
IT1304500B1 IT1998MI002792A ITMI982792A IT1304500B1 IT 1304500 B1 IT1304500 B1 IT 1304500B1 IT 1998MI002792 A IT1998MI002792 A IT 1998MI002792A IT MI982792 A ITMI982792 A IT MI982792A IT 1304500 B1 IT1304500 B1 IT 1304500B1
Authority
IT
Italy
Prior art keywords
recombined
bacterial
strips
production
natural nucleosides
Prior art date
Application number
IT1998MI002792A
Other languages
English (en)
Inventor
Giuseppina Bestetti
Simona Cali
Daniela Ghisotti
Gaetano Orsini
Giancarlo Tonon
Gabriele Zuffi
Original Assignee
Norpharma Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to IT1998MI002792A priority Critical patent/IT1304500B1/it
Application filed by Norpharma Spa filed Critical Norpharma Spa
Priority to ES99965565T priority patent/ES2229807T3/es
Priority to JP2000591198A priority patent/JP2002533126A/ja
Priority to PCT/EP1999/010416 priority patent/WO2000039307A2/en
Priority to AT99965565T priority patent/ATE278021T1/de
Priority to DE69920749T priority patent/DE69920749T2/de
Priority to AU21030/00A priority patent/AU2103000A/en
Priority to EP99965565A priority patent/EP1141328B1/en
Publication of ITMI982792A1 publication Critical patent/ITMI982792A1/it
Application granted granted Critical
Publication of IT1304500B1 publication Critical patent/IT1304500B1/it
Priority to US09/891,865 priority patent/US6911341B2/en
Priority to US11/036,497 priority patent/US7781189B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/38—Nucleosides
    • C12P19/40—Nucleosides having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same ring, e.g. purine nucleosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/38—Nucleosides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
IT1998MI002792A 1998-12-23 1998-12-23 Ceppi batterici ricombinati per la produzione di nucleosidi naturali edi analoghi modificati. IT1304500B1 (it)

Priority Applications (10)

Application Number Priority Date Filing Date Title
IT1998MI002792A IT1304500B1 (it) 1998-12-23 1998-12-23 Ceppi batterici ricombinati per la produzione di nucleosidi naturali edi analoghi modificati.
JP2000591198A JP2002533126A (ja) 1998-12-23 1999-12-23 天然ヌクレオシドおよびその改変類縁体を産生するための組換え細菌株
PCT/EP1999/010416 WO2000039307A2 (en) 1998-12-23 1999-12-23 Recombinant bacterial strains for the production of natural nucleosides and modified analogues thereof
AT99965565T ATE278021T1 (de) 1998-12-23 1999-12-23 Rekombinante bakterienstämme zur herstellung von natürlichen nucleosiden, sowie von modifizierten analogen davon
ES99965565T ES2229807T3 (es) 1998-12-23 1999-12-23 Cepas bacterianas recombinantes para la produccion de nucleosidos naturales y analogos modificados de los mismos.
DE69920749T DE69920749T2 (de) 1998-12-23 1999-12-23 Rekombinante bakterienstämme zur herstellung von natürlichen nucleosiden, sowie von modifizierten analogen davon
AU21030/00A AU2103000A (en) 1998-12-23 1999-12-23 Recombinant bacterial strains for the production of natural nucleosides and modified analogues thereof
EP99965565A EP1141328B1 (en) 1998-12-23 1999-12-23 Recombinant bacterial strains for the production of natural nucleosides and modified analogues thereof
US09/891,865 US6911341B2 (en) 1998-12-23 2001-06-25 Vectors, host cells, and methods for production of uridine phosphorylase and purine nucleoside phosphorylase
US11/036,497 US7781189B2 (en) 1998-12-23 2005-01-14 Catalyzing transglycosylation using a recombinant host cell overexpressing uridine phosphorylase and purine nucleoside phosphorylase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT1998MI002792A IT1304500B1 (it) 1998-12-23 1998-12-23 Ceppi batterici ricombinati per la produzione di nucleosidi naturali edi analoghi modificati.

Publications (2)

Publication Number Publication Date
ITMI982792A1 ITMI982792A1 (it) 2000-06-23
IT1304500B1 true IT1304500B1 (it) 2001-03-19

Family

ID=11381317

Family Applications (1)

Application Number Title Priority Date Filing Date
IT1998MI002792A IT1304500B1 (it) 1998-12-23 1998-12-23 Ceppi batterici ricombinati per la produzione di nucleosidi naturali edi analoghi modificati.

Country Status (9)

Country Link
US (2) US6911341B2 (it)
EP (1) EP1141328B1 (it)
JP (1) JP2002533126A (it)
AT (1) ATE278021T1 (it)
AU (1) AU2103000A (it)
DE (1) DE69920749T2 (it)
ES (1) ES2229807T3 (it)
IT (1) IT1304500B1 (it)
WO (1) WO2000039307A2 (it)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1304500B1 (it) * 1998-12-23 2001-03-19 Norpharma Spa Ceppi batterici ricombinati per la produzione di nucleosidi naturali edi analoghi modificati.
US7037718B2 (en) * 2001-10-26 2006-05-02 Cornell Research Foundation, Inc. Mutant purine nucleoside phosphorylase proteins and cellular delivery thereof
US7488598B2 (en) 2001-10-26 2009-02-10 Cornell Center For Technology Enterprise And Commercialization Mutant purine nucleoside phosphorylase proteins and cellular delivery thereof
ES2235638B1 (es) * 2003-12-17 2006-10-16 Cellerix, S.L. Nuevo vector retroviral de alto titulo y metodos de transduccion.
PT1835035E (pt) * 2006-03-15 2010-05-26 Explora Lab Sa Processo para a imobilização de células em resina
EP2338985A1 (en) * 2009-12-22 2011-06-29 Institut Univ. de Ciència i Tecnologia, s.a. Thermostable biocatalyst combination for nucleoside synthesis
JP2016198075A (ja) * 2015-04-14 2016-12-01 プラズミア バイオテック，エス．エル． シトシンヌクレオシドアナログの酵素産生
CN113969299B (zh) * 2021-10-17 2023-10-20 拓新药业集团股份有限公司 一种生物转化合成尿苷的方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1573777A (en) * 1977-11-03 1980-08-28 Wellcome Found 9-d-arabinonucleosides and an enzymatic process for their preparation
JPS56164793A (en) * 1980-04-23 1981-12-17 Wellcome Found Pyridine derivative
US4347315A (en) * 1980-04-25 1982-08-31 Burroughs Wellcome Co. Synthesis of ribosides using bacterial phosphorylase
US4835104A (en) 1987-06-16 1989-05-30 Ajinomoto Co., Inc., Patent & Licensing Department Process for producing and purifying 2',3'-dideoxynucleosides, and process for producing 2',3'-dideoxy-2',3'-didehydronucleosides
JP2619710B2 (ja) * 1989-02-27 1997-06-11 日本製紙 株式会社 ２′，３′−ジデオキシプリンヌクレオシド類の製造方法
EP0423641A3 (en) * 1989-10-18 1992-01-15 Nippon Mining Company Limited Expression vector, transformed microorganism, fusion protein and method for the production of platelet factor 4 or tgfalpha
JPH06253854A (ja) * 1993-02-26 1994-09-13 Yamasa Shoyu Co Ltd 組換えｄｎａ手法によるヌクレオシド・ホスホリラーゼの製造法
JP4304727B2 (ja) * 1996-11-21 2009-07-29 味の素株式会社 ヌクレオシド−５’−燐酸エステルの製造法
US6229001B1 (en) * 1997-01-03 2001-05-08 Southern Research Institute Mycobacterium fol a gene that encodes for the enzyme dihydrofolate reductase
JPH10286097A (ja) * 1997-04-15 1998-10-27 Yamasa Shoyu Co Ltd プリンアラビノシドの酵素的製造法
JP3799784B2 (ja) 1997-11-14 2006-07-19 味の素株式会社 ２，６−ジアミノプリン−２’−デオキシリボシドおよび２’−デオキシグアノシンの製造法
IT1304500B1 (it) * 1998-12-23 2001-03-19 Norpharma Spa Ceppi batterici ricombinati per la produzione di nucleosidi naturali edi analoghi modificati.

Also Published As

Publication number Publication date
DE69920749T2 (de) 2006-03-02
DE69920749D1 (de) 2004-11-04
US6911341B2 (en) 2005-06-28
JP2002533126A (ja) 2002-10-08
ATE278021T1 (de) 2004-10-15
EP1141328A2 (en) 2001-10-10
WO2000039307A2 (en) 2000-07-06
US20050142645A1 (en) 2005-06-30
US7781189B2 (en) 2010-08-24
AU2103000A (en) 2000-07-31
ITMI982792A1 (it) 2000-06-23
WO2000039307A3 (en) 2000-11-09
ES2229807T3 (es) 2005-04-16
EP1141328B1 (en) 2004-09-29
US20030059870A1 (en) 2003-03-27

Similar Documents

Publication Publication Date Title
Berens et al. Purine metabolism in Trypanosoma cruzi
IT1294728B1 (it) Ceppi di lievito per la riproduzione di acido lattico
WO1995007718A3 (en) Purine nucleoside phosphorylase gene therapy for human malignancy
AU1603899A (en) Mutant (bacillus licheniformis) alpha-amylase
WO1998020145A3 (en) Improvements in or relating to starch content of plants
NO885560D0 (no) Astaxanthin-produserende gjaerceller, metoder dor deres fremstilling og deres anvendelser.
EP0905232A4 (en) NEW BETA 1-4 N-ACETYLGLUCOSAMINYL TRANSFERASE AND THE GENE CODING FOR THESE
IT1304500B1 (it) Ceppi batterici ricombinati per la produzione di nucleosidi naturali edi analoghi modificati.
KR930016543A (ko) 시티딘 디인산 콜린의 제조방법
WO2003035012A3 (en) Mutant purine nucleoside phosphorylase proteins and cellular delivery thereof
Utagawa et al. Properties of nucleoside phosphorylase from Enterobacter aerogenes
DK197487A (da) System til biotinsyntese
DE59812281D1 (en) Promotor aus ashbya gossypii
ES8701224A1 (es) Un metodo para producir citidina y-o desoxicitidina
AU5108090A (en) Production of nucleoside
Nóbile et al. Aeromonas hydrophila strains as biocatalysts for transglycosylation
DK0751218T3 (da) Saccharose-stofskifte-mutanter
ATE42341T1 (de) Herstellung von uridin.
ES2181897T3 (es) Composicion enzimatica.
Avraham et al. Thymidine phosphorylase and uridine phosphorylase of Lactobacillus casei
PT1001957E (pt) Macrolidos com actividade antitumoral
Barbes et al. DNA-methyltransferase activities in Streptomyces antibioticus
ES2188703T3 (es) Procedimiento para la sintesis enzimatica de nucleotido-6-desoxi-d-xilo-4-hexulosas.
NADKARNI et al. Raffinose metabolism and a novel mode of sucrose synthesis in Corynebacterium murisepticum
MOORE et al. Synthesis of two distinct α‐glucosidase activities in Bacillus licheniformis