DE69739898D1 - Tests auf proteinkinasen mit fluoreszierenden proteinsubstraten - Google Patents
Tests auf proteinkinasen mit fluoreszierenden proteinsubstratenInfo
- Publication number
- DE69739898D1 DE69739898D1 DE69739898T DE69739898T DE69739898D1 DE 69739898 D1 DE69739898 D1 DE 69739898D1 DE 69739898 T DE69739898 T DE 69739898T DE 69739898 T DE69739898 T DE 69739898T DE 69739898 D1 DE69739898 D1 DE 69739898D1
- Authority
- DE
- Germany
- Prior art keywords
- protein
- testing
- kinas
- substrates
- phosphorylated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- G01N2333/91205—Phosphotransferases in general
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- General Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US68087796A | 1996-07-16 | 1996-07-16 | |
US08/679,865 US5912137A (en) | 1996-07-16 | 1996-07-16 | Assays for protein kinases using fluorescent |
US08/680,876 US5925558A (en) | 1996-07-16 | 1996-07-16 | Assays for protein kinases using fluorescent protein substrates |
PCT/US1997/012410 WO1998002571A1 (en) | 1996-07-16 | 1997-07-16 | Assays for protein kinases using fluorescent protein substrates |
Publications (1)
Publication Number | Publication Date |
---|---|
DE69739898D1 true DE69739898D1 (de) | 2010-07-15 |
Family
ID=27418360
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69739898T Expired - Lifetime DE69739898D1 (de) | 1996-07-16 | 1997-07-16 | Tests auf proteinkinasen mit fluoreszierenden proteinsubstraten |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP0915989B1 (de) |
AT (1) | ATE469981T1 (de) |
AU (1) | AU3801997A (de) |
DE (1) | DE69739898D1 (de) |
WO (1) | WO1998002571A1 (de) |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8669074B2 (en) | 1996-01-31 | 2014-03-11 | The Regents Of The University Of California | Chimeric phosphorylation indicator |
US6900304B2 (en) * | 1996-01-31 | 2005-05-31 | The Regents Of The University Of California | Emission ratiometric indicators of phosphorylation |
WO1998040477A1 (en) * | 1997-03-14 | 1998-09-17 | The Regents Of The University Of California | Fluorescent protein sensors for detection of analytes |
US6197928B1 (en) | 1997-03-14 | 2001-03-06 | The Regents Of The University Of California | Fluorescent protein sensors for detection of analytes |
WO1998045704A2 (en) * | 1997-04-07 | 1998-10-15 | Bioimage A/S | A method for extracting quantitative information relating to an influence on a cellular response |
US7105307B2 (en) | 1997-08-30 | 2006-09-12 | Cyclacel, Ltd. | Compositions and methods for screening for modulators of enzymatic activity |
FR2768817B1 (fr) | 1997-09-19 | 1999-12-10 | Cis Bio Int | Methode homogene pour la detection et/ou la determination de l'activite phosphorylante d'un materiel biologique |
EP1925320A3 (de) | 1998-03-27 | 2008-09-03 | Prolume, Ltd. | Luciferasen, fluoreszierende Proteine, Nukleinsäuren zur Kodierung der Luciferasen und der fluoreszierenden Proteine sowie deren Verwendung zur Diagnose, zum Screening mit hohem Durchsatz und zur Behandlung neuartiger Probleme |
US6150176A (en) * | 1998-06-09 | 2000-11-21 | The Regents Of The University Of California | Fluorescent protein sensors for measuring the pH of a biological sample |
JP2002526756A (ja) * | 1998-09-24 | 2002-08-20 | デューク ユニバーシティ | 生細胞におけるタンパク質−タンパク質相互作用の測定方法 |
JP2002527761A (ja) * | 1998-10-15 | 2002-08-27 | バイオイメージ エイ/エス | 細胞応答への影響に関する量的情報を入手するための改良方法 |
GB9904407D0 (en) * | 1999-02-25 | 1999-04-21 | Fluorescience Ltd | Compositions and methods for monitoring the modification of engineered binding partners |
EP1163363B1 (de) * | 1999-02-25 | 2006-07-19 | Cyclacel Limited | Verfahren und zusammensetzungen zur überwachung der modifikation von natürlichen bindungspartners |
US6656696B2 (en) | 1999-02-26 | 2003-12-02 | Cyclacel | Compositions and methods for monitoring the phosphorylation of natural binding partners |
NL1012782C2 (nl) * | 1999-08-05 | 2001-02-06 | Keygene Nv | Verbeterde gfp-variant, bruikbaar als markering, en gfp-construct. |
ATE378400T1 (de) | 2001-02-14 | 2007-11-15 | Sysmex Corp | Verfahren zur bestimmung der aktivität des zellzyklus-regulationsfaktors und verfahren zur diagnose von krebs unter verwendung desselben |
AU2002254173A1 (en) | 2001-03-12 | 2002-10-08 | The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The Unive | Oxidation-reduction sensitive green fluorescent protein variants |
JP3829252B2 (ja) | 2001-06-08 | 2006-10-04 | 独立行政法人理化学研究所 | 蛍光蛋白質 |
DE60234131D1 (de) | 2001-08-01 | 2009-12-03 | Cellomics Inc | Neue fusionsproteine und molekulare bindungsassays |
FR2828692B1 (fr) * | 2001-08-16 | 2004-07-09 | France Etat Armement | Procede d'ingenierie genetique pour le suivi en temps reel de l'activation de proteines specifiques de transduction de signaux cellulaires |
GB0208987D0 (en) * | 2002-04-19 | 2002-05-29 | Amersham Biosciences Uk Ltd | Methods for measuring protein kinase and phosphatase activity |
US7727752B2 (en) | 2003-07-29 | 2010-06-01 | Life Technologies Corporation | Kinase and phosphatase assays |
GB0421285D0 (en) | 2004-09-24 | 2004-10-27 | Univ Nottingham | Improvements in high content screening |
US8680235B2 (en) | 2006-09-22 | 2014-03-25 | Stowers Institute For Medical Research | Branchiostoma derived fluorescent proteins |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8916806D0 (en) * | 1989-07-22 | 1989-09-06 | Univ Wales Medicine | Modified proteins |
US5723286A (en) * | 1990-06-20 | 1998-03-03 | Affymax Technologies N.V. | Peptide library and screening systems |
US5532167A (en) * | 1994-01-07 | 1996-07-02 | Beth Israel Hospital | Substrate specificity of protein kinases |
ES2170220T3 (es) * | 1995-01-31 | 2002-08-01 | Bioimage As | Procedimiento para detectar sustancias biologicamente activas. |
-
1997
- 1997-07-16 AT AT97934976T patent/ATE469981T1/de not_active IP Right Cessation
- 1997-07-16 EP EP97934976A patent/EP0915989B1/de not_active Expired - Lifetime
- 1997-07-16 DE DE69739898T patent/DE69739898D1/de not_active Expired - Lifetime
- 1997-07-16 AU AU38019/97A patent/AU3801997A/en not_active Abandoned
- 1997-07-16 WO PCT/US1997/012410 patent/WO1998002571A1/en active Application Filing
Also Published As
Publication number | Publication date |
---|---|
WO1998002571A1 (en) | 1998-01-22 |
EP0915989A4 (de) | 2005-10-12 |
EP0915989A1 (de) | 1999-05-19 |
EP0915989B1 (de) | 2010-06-02 |
ATE469981T1 (de) | 2010-06-15 |
AU3801997A (en) | 1998-02-09 |
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