DE69032630D1 - Verfahren zur Herstellung von Restriktionsendonuklease XmaI - Google Patents
Verfahren zur Herstellung von Restriktionsendonuklease XmaIInfo
- Publication number
- DE69032630D1 DE69032630D1 DE69032630T DE69032630T DE69032630D1 DE 69032630 D1 DE69032630 D1 DE 69032630D1 DE 69032630 T DE69032630 T DE 69032630T DE 69032630 T DE69032630 T DE 69032630T DE 69032630 D1 DE69032630 D1 DE 69032630D1
- Authority
- DE
- Germany
- Prior art keywords
- preparation
- restriction endonuclease
- xmai
- endonuclease xmai
- restriction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
- C12N9/1007—Methyltransferases (general) (2.1.1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/344,268 US5002882A (en) | 1989-04-27 | 1989-04-27 | Method for producing the XmaI restriction endonuclease and methylase |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69032630D1 true DE69032630D1 (de) | 1998-10-15 |
DE69032630T2 DE69032630T2 (de) | 1999-03-25 |
Family
ID=23349779
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE90304413T Pending DE395367T1 (de) | 1989-04-27 | 1990-04-25 | Verfahren zur Herstellung von Restriktionsendonuklease und -methylase XamI. |
DE69032630T Expired - Lifetime DE69032630T2 (de) | 1989-04-27 | 1990-04-25 | Verfahren zur Herstellung von Restriktionsendonuklease XmaI |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE90304413T Pending DE395367T1 (de) | 1989-04-27 | 1990-04-25 | Verfahren zur Herstellung von Restriktionsendonuklease und -methylase XamI. |
Country Status (4)
Country | Link |
---|---|
US (1) | US5002882A (de) |
EP (1) | EP0395367B1 (de) |
JP (1) | JP2952003B2 (de) |
DE (2) | DE395367T1 (de) |
Families Citing this family (55)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5298404A (en) * | 1989-10-13 | 1994-03-29 | New England Biolabs, Inc. | Method for producing the Hpa I restriction endonuclease and methylase |
US5192676A (en) * | 1991-02-05 | 1993-03-09 | New England Biolabs, Inc. | Type ii restriction endonuclease, asci, obtainable from arthrobacter species and a process for producing the same |
US5200337A (en) * | 1991-10-25 | 1993-04-06 | New England Biolabs, Inc. | Type ii restriction endonuclease, apo i, obtainable from arthrobacter protophormiae and a process for producing the same |
EP0555797A1 (de) * | 1992-02-10 | 1993-08-18 | Becton, Dickinson and Company | Universalpufferlösung für Restriktionsendonukleasen |
US5231021A (en) * | 1992-04-10 | 1993-07-27 | Life Technologies, Inc. | Cloning and expressing restriction endonucleases and modification methylases from xanthomonas |
US5248605A (en) * | 1992-12-07 | 1993-09-28 | Life Technologies, Inc. | Cloning and expressing restriction endonucleases from haemophilus |
US5312746A (en) * | 1993-01-08 | 1994-05-17 | Life Technologies, Inc. | Cloning and expressing restriction endonucleases and modification methylases from caryophanon |
US5334526A (en) * | 1993-05-28 | 1994-08-02 | Life Technologies, Inc. | Cloning and expression of AluI restriction endonuclease |
WO1996007803A1 (en) * | 1994-09-08 | 1996-03-14 | Non-Compact, Inc. | System for mounting building panels |
EP2548953A1 (de) | 2005-08-04 | 2013-01-23 | New England Biolabs, Inc. | Neuartige Restriktionsendonukleasen, DNA-Kodierung dieser Endonukleasen und Verfahren zur Identifizierung neuer Endonukleasen mit der gleichen oder variierten Spezifizität |
US9085798B2 (en) | 2009-04-30 | 2015-07-21 | Prognosys Biosciences, Inc. | Nucleic acid constructs and methods of use |
US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
HUE026666T2 (en) | 2010-04-05 | 2016-07-28 | Prognosys Biosciences Inc | Spatially encoded biological assays |
WO2012139110A2 (en) | 2011-04-08 | 2012-10-11 | Prognosys Biosciences, Inc. | Peptide constructs and assay systems |
GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
EP3736573A1 (de) | 2013-03-15 | 2020-11-11 | Prognosys Biosciences, Inc. | Verfahren zum nachweis von peptid/mhc/tcr-bindung |
CN111662960B (zh) | 2013-06-25 | 2024-04-12 | 普罗格诺西斯生物科学公司 | 采用微流控装置的空间编码生物分析 |
WO2015070037A2 (en) | 2013-11-08 | 2015-05-14 | Prognosys Biosciences, Inc. | Polynucleotide conjugates and methods for analyte detection |
CN113186256A (zh) | 2015-04-10 | 2021-07-30 | 空间转录公司 | 生物样本的空间区别、多重核酸分析 |
US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
US20220033888A1 (en) | 2018-12-10 | 2022-02-03 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
WO2020243579A1 (en) | 2019-05-30 | 2020-12-03 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
WO2021092433A2 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
WO2021091611A1 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Spatially-tagged analyte capture agents for analyte multiplexing |
FI3891300T3 (fi) | 2019-12-23 | 2023-05-10 | 10X Genomics Inc | Menetelmät spatiaalista analyysiä varten rna-templatoitua ligaatiota käyttäen |
US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
US11821035B1 (en) | 2020-01-29 | 2023-11-21 | 10X Genomics, Inc. | Compositions and methods of making gene expression libraries |
US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
US11835462B2 (en) | 2020-02-11 | 2023-12-05 | 10X Genomics, Inc. | Methods and compositions for partitioning a biological sample |
US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
US11926863B1 (en) | 2020-02-27 | 2024-03-12 | 10X Genomics, Inc. | Solid state single cell method for analyzing fixed biological cells |
US11768175B1 (en) | 2020-03-04 | 2023-09-26 | 10X Genomics, Inc. | Electrophoretic methods for spatial analysis |
CN115916999A (zh) | 2020-04-22 | 2023-04-04 | 10X基因组学有限公司 | 用于使用靶向rna耗竭进行空间分析的方法 |
WO2021237087A1 (en) | 2020-05-22 | 2021-11-25 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
WO2021236929A1 (en) | 2020-05-22 | 2021-11-25 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
WO2021242834A1 (en) | 2020-05-26 | 2021-12-02 | 10X Genomics, Inc. | Method for resetting an array |
AU2021283184A1 (en) | 2020-06-02 | 2023-01-05 | 10X Genomics, Inc. | Spatial transcriptomics for antigen-receptors |
WO2021247543A2 (en) | 2020-06-02 | 2021-12-09 | 10X Genomics, Inc. | Nucleic acid library methods |
US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
EP4162074B1 (de) | 2020-06-08 | 2024-04-24 | 10X Genomics, Inc. | Verfahren zur bestimmung eines chirurgischen randes und verfahren zur verwendung davon |
WO2021252591A1 (en) | 2020-06-10 | 2021-12-16 | 10X Genomics, Inc. | Methods for determining a location of an analyte in a biological sample |
WO2021263111A1 (en) | 2020-06-25 | 2021-12-30 | 10X Genomics, Inc. | Spatial analysis of dna methylation |
US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
WO2022140028A1 (en) | 2020-12-21 | 2022-06-30 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
EP4301870A1 (de) | 2021-03-18 | 2024-01-10 | 10X Genomics, Inc. | Multiplex-erfassung von gen- und proteinexpression aus einer biologischen probe |
WO2023034489A1 (en) | 2021-09-01 | 2023-03-09 | 10X Genomics, Inc. | Methods, compositions, and kits for blocking a capture probe on a spatial array |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IN166864B (de) * | 1985-03-01 | 1990-07-28 | New England Biolabs Inc |
-
1989
- 1989-04-27 US US07/344,268 patent/US5002882A/en not_active Expired - Lifetime
-
1990
- 1990-04-25 DE DE90304413T patent/DE395367T1/de active Pending
- 1990-04-25 DE DE69032630T patent/DE69032630T2/de not_active Expired - Lifetime
- 1990-04-25 EP EP90304413A patent/EP0395367B1/de not_active Expired - Lifetime
- 1990-04-27 JP JP2115010A patent/JP2952003B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DE395367T1 (de) | 1994-02-03 |
EP0395367B1 (de) | 1998-09-09 |
EP0395367A1 (de) | 1990-10-31 |
US5002882A (en) | 1991-03-26 |
JP2952003B2 (ja) | 1999-09-20 |
JPH0361491A (ja) | 1991-03-18 |
DE69032630T2 (de) | 1999-03-25 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |