DE68917642D1 - Verfahren zur kontinuierlichen Züchtung von haftenden tierischen Zellen. - Google Patents

Verfahren zur kontinuierlichen Züchtung von haftenden tierischen Zellen.

Info

Publication number
DE68917642D1
DE68917642D1 DE68917642T DE68917642T DE68917642D1 DE 68917642 D1 DE68917642 D1 DE 68917642D1 DE 68917642 T DE68917642 T DE 68917642T DE 68917642 T DE68917642 T DE 68917642T DE 68917642 D1 DE68917642 D1 DE 68917642D1
Authority
DE
Germany
Prior art keywords
animal cells
continuous cultivation
adherent animal
adherent
cultivation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE68917642T
Other languages
English (en)
Other versions
DE68917642T2 (de
Inventor
Yoshiharu Takazawa
Michiyuki Tokashiki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Teijin Ltd
Original Assignee
Teijin Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP12566488A external-priority patent/JPH01296986A/ja
Priority claimed from JP63204733A external-priority patent/JPH084495B2/ja
Application filed by Teijin Ltd filed Critical Teijin Ltd
Application granted granted Critical
Publication of DE68917642D1 publication Critical patent/DE68917642D1/de
Publication of DE68917642T2 publication Critical patent/DE68917642T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE68917642T 1988-05-25 1989-05-24 Verfahren zur kontinuierlichen Züchtung von haftenden tierischen Zellen. Expired - Fee Related DE68917642T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP12566488A JPH01296986A (ja) 1988-05-25 1988-05-25 接着性動物細胞の培養方法
JP63204733A JPH084495B2 (ja) 1988-08-19 1988-08-19 接着性動物細胞の培養方法

Publications (2)

Publication Number Publication Date
DE68917642D1 true DE68917642D1 (de) 1994-09-29
DE68917642T2 DE68917642T2 (de) 1995-02-09

Family

ID=26462027

Family Applications (1)

Application Number Title Priority Date Filing Date
DE68917642T Expired - Fee Related DE68917642T2 (de) 1988-05-25 1989-05-24 Verfahren zur kontinuierlichen Züchtung von haftenden tierischen Zellen.

Country Status (2)

Country Link
EP (1) EP0343635B1 (de)
DE (1) DE68917642T2 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4115722A1 (de) * 1991-05-14 1992-11-19 Boehringer Mannheim Gmbh Serumfreies medium zur kultivierung von saeugerzellen
GB9118664D0 (en) * 1991-08-30 1991-10-16 Celltech Ltd Cell culture
WO1993022448A1 (en) * 1992-05-01 1993-11-11 Teijin Limited Fed batch culture method for protein secreting cells
EP0653487A1 (de) * 1993-11-07 1995-05-17 Ferruccio Dr. Messi Serum- und proteinfrei wachsende Zellen
WO1998004681A2 (en) * 1996-07-25 1998-02-05 Genzyme Corporation Chondrocyte media formulations and culture procedures
JO2291B1 (en) 1999-07-02 2005-09-12 اف . هوفمان لاروش ايه جي Erythropoietin derivatives
AU2002233230B2 (en) 2000-12-20 2007-02-01 F. Hoffmann-La Roche Ag Erythropoietin conjugates
US8206981B2 (en) 2004-03-05 2012-06-26 Dsm Ip Assets B.V. Process for cell culturing by continuous perfusion and alternating tangential flow
KR20090046923A (ko) 2006-08-04 2009-05-11 프로롱 파마슈티컬스, 인크. 수식된 에리스로포이에틴
UA107678C2 (uk) 2009-09-23 2015-02-10 ратіофарм ГмбХ Процес очищення рекомбінантного людського еритропоетину (епо), епо, очищений таким чином, та фармацевтична композиція, що містить його

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2543158B1 (fr) * 1983-03-24 1985-11-15 Inst Nat Sante Rech Med Milieu de culture de cellules animales sans serum, sans hormones et sans facteurs de croissance et procedes de culture primaire et d'obtention de lignees cellulaires utilisant ce milieu
US4673649A (en) * 1983-07-15 1987-06-16 University Patents, Inc. Process and defined medium for growth of human epidermal keratinocyte cells
US4767704A (en) * 1983-10-07 1988-08-30 Columbia University In The City Of New York Protein-free culture medium
JPS62134086A (ja) * 1985-12-06 1987-06-17 Teijin Ltd 動物細胞の培養方法
DE3787805T2 (de) * 1986-08-04 1994-02-10 Garvan Inst Med Res Serumfreies gewebekulturmedium, das ein polymerzellenschutzmittel enthält.

Also Published As

Publication number Publication date
EP0343635B1 (de) 1994-08-24
DE68917642T2 (de) 1995-02-09
EP0343635A3 (en) 1990-05-16
EP0343635A2 (de) 1989-11-29

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee