DE60331311D1 - Expressionsvektor, wirt, fusionsprotein, verfahren zur herstellung von fusionsprotein und verfahren zur herstellung von protein - Google Patents

Expressionsvektor, wirt, fusionsprotein, verfahren zur herstellung von fusionsprotein und verfahren zur herstellung von protein

Info

Publication number
DE60331311D1
DE60331311D1 DE60331311T DE60331311T DE60331311D1 DE 60331311 D1 DE60331311 D1 DE 60331311D1 DE 60331311 T DE60331311 T DE 60331311T DE 60331311 T DE60331311 T DE 60331311T DE 60331311 D1 DE60331311 D1 DE 60331311D1
Authority
DE
Germany
Prior art keywords
protein
coding region
production
expression vector
fusion protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60331311T
Other languages
English (en)
Inventor
Akira Ideno
Tadashi Maruyama
Masahiro Furutani
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sekisui Chemical Co Ltd
Original Assignee
Sekisui Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sekisui Chemical Co Ltd filed Critical Sekisui Chemical Co Ltd
Application granted granted Critical
Publication of DE60331311D1 publication Critical patent/DE60331311D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DE60331311T 2002-06-25 2003-06-25 Expressionsvektor, wirt, fusionsprotein, verfahren zur herstellung von fusionsprotein und verfahren zur herstellung von protein Expired - Fee Related DE60331311D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2002185020 2002-06-25
PCT/JP2003/008020 WO2004001041A1 (ja) 2002-06-25 2003-06-25 発現ベクター、宿主、融合タンパク質、融合タンパク質の製造方法及びタンパク質の製造方法

Publications (1)

Publication Number Publication Date
DE60331311D1 true DE60331311D1 (de) 2010-04-01

Family

ID=29996719

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60331311T Expired - Fee Related DE60331311D1 (de) 2002-06-25 2003-06-25 Expressionsvektor, wirt, fusionsprotein, verfahren zur herstellung von fusionsprotein und verfahren zur herstellung von protein

Country Status (8)

Country Link
US (1) US20050130259A1 (de)
EP (1) EP1516928B1 (de)
JP (1) JP4168028B2 (de)
AT (1) ATE458059T1 (de)
AU (1) AU2003243969B2 (de)
CA (1) CA2490384A1 (de)
DE (1) DE60331311D1 (de)
WO (1) WO2004001041A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4988337B2 (ja) * 2004-05-21 2012-08-01 タカラバイオ株式会社 ポリペプチドの製造方法
JP2006230251A (ja) * 2005-02-23 2006-09-07 Kagoshima Univ 融合タンパク質発現ベクター
ATE527345T1 (de) 2006-01-03 2011-10-15 Hoffmann La Roche Chimäres fusionsprotein mit überlegenen chaperon- und faltungsaktivitäten
JP2010235492A (ja) * 2009-03-31 2010-10-21 Sekisui Chem Co Ltd シャペロニン−目的タンパク質複合体及びその製造方法
AR086250A1 (es) 2011-05-05 2013-11-27 Hoffmann La Roche Polipeptido de fusion presentador de una secuencia de aminoacidos y utilizacion del mismo
WO2014071978A1 (en) 2012-11-08 2014-05-15 Roche Diagnostics Gmbh Nucleic acids encoding chimeric polypeptides for library screening
US10414818B2 (en) 2013-09-27 2019-09-17 Roche Diagnostics Operations, Inc. Thermus thermophilus SlyD FKBP domain specific antibodies
CN107530410B (zh) * 2015-03-18 2021-11-09 欧姆尼赛特有限公司 包含经修饰的α病毒表面糖蛋白与肿瘤相关抗原的融合蛋白及其方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997010253A1 (en) * 1995-09-15 1997-03-20 Merck & Co., Inc. A high throughput assay using fusion proteins
US5989868A (en) * 1997-09-12 1999-11-23 The Board Of Regents Of The University Of Oklahoma Fusion protein systems designed to increase soluble cytoplasmic expression of heterologous proteins in esherichia coli
DE69841767D1 (de) * 1998-10-02 2010-08-26 Rhein Biotech Proz & Prod Gmbh Methode zur Herstellung von (Poly)peptiden unter Verwendung von verkürzten Varianten von SV40 Large T antigen mit einem intakten N Terminus
GB9913437D0 (en) * 1999-06-09 1999-08-11 Medical Res Council Fusion proteins
JP2002306182A (ja) * 2000-05-26 2002-10-22 Toyota Central Res & Dev Lab Inc 抗菌蛋白質の製造方法、蛋白質融合体
JP2002262883A (ja) * 2001-03-13 2002-09-17 Sekisui Chem Co Ltd モノクローナル抗体の製造方法
DE60233942D1 (de) * 2001-06-22 2009-11-19 Hoffmann La Roche Verwendung von fkbp-chaperons als expressionswerkzeug

Also Published As

Publication number Publication date
AU2003243969B2 (en) 2008-03-13
EP1516928A1 (de) 2005-03-23
ATE458059T1 (de) 2010-03-15
AU2003243969A1 (en) 2004-01-06
JP4168028B2 (ja) 2008-10-22
EP1516928A4 (de) 2006-11-08
US20050130259A1 (en) 2005-06-16
EP1516928B1 (de) 2010-02-17
WO2004001041A1 (ja) 2003-12-31
JPWO2004001041A1 (ja) 2005-10-20
CA2490384A1 (en) 2003-12-31

Similar Documents

Publication Publication Date Title
DE60018909D1 (de) Neue carbonyl-reduktase, gen davon und verfahren der anwendung derselben
DE69942280D1 (de) Zusammensetzungen und verfahren zur verpackung von alpavirus-vektoren
DK1346034T3 (da) von Willebrand-faktor (vWF) spaltende protease-polypeptid, nukleinsyre kodende for polypeptidet og anvendelse af polypeptidet
DE60331226D1 (de) Verfahren und dna-konstrukte zur produktion von polypeptiden mit hoher ausbeute
DE60227069D1 (de) VERFAHREN UND ZUSAMMENSETZUNGEN ZUR MODIFIKATION DER BEARBEITUNG VON APOLIPOPROTEIN B-mRNA
DE60238472D1 (de) Abschwächung der immunogenizität von fusionsproteinen
EE200200538A (et) Rekombinantsete verehüübimisfaktorite produtseerimine inimese rakuliinides
ATE458059T1 (de) Expressionsvektor, wirt, fusionsprotein, verfahren zur herstellung von fusionsprotein und verfahren zur herstellung von protein
DK1826271T3 (da) Termofilt svampeekspressionssystem
ATE524559T1 (de) Methode zur verbesserung der proteinproduktion
DE69943393D1 (de) Ein plasmaprotein-fragment erzeugendes enzym mit krebsmetastase-hemmender wirkung und durch dieses enzym fragmentiertes plasmaprotein-fragment
DK0866848T3 (da) Forbedrede neurospora-værter til fremstilling af rekombinante proteiner og fremgangsmåde til fremstilling af samme
DE60211772D1 (de) Verfahren zur Herstellung von Proteine
FI930626A (fi) Proteinstruktur hos vaexttoxingelonin
ATE532793T1 (de) Verfahren zur herstellung von molekülen mit verminderter immunogenität
ATE389010T1 (de) Verfahren zur herstellung von optisch aktiven pyridinethanol-derivaten
DE60327228D1 (de) Verfahren zur herstellung von wachstumshormon und antagonisten davon mit geringeren konzentrationen an isoformen verunreinigungen davon
EP1634954A4 (de) Protease, für die protease codierende dna und verfahren zur herstellung der protease
KR930008148A (ko) 변이 인간 프로우로키나아제
DE60040771D1 (de) Verfahren zur kontrolle der spaltung mit ompt protease
NO20005819L (no) Nye peptidfragmenter for rensing av proteiner
ATE482274T1 (de) Polypeptid mit amidaseaktivität und dessen gen
ATE449857T1 (de) Koexpressionsvektor für die membrandomänen von virushüllproteinen und verwendungen davon

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee