DE602006019203D1 - Probenherstellungsverfahren mit einem alkali-stoss - Google Patents

Probenherstellungsverfahren mit einem alkali-stoss

Info

Publication number
DE602006019203D1
DE602006019203D1 DE602006019203T DE602006019203T DE602006019203D1 DE 602006019203 D1 DE602006019203 D1 DE 602006019203D1 DE 602006019203 T DE602006019203 T DE 602006019203T DE 602006019203 T DE602006019203 T DE 602006019203T DE 602006019203 D1 DE602006019203 D1 DE 602006019203D1
Authority
DE
Germany
Prior art keywords
biological sample
nucleic acid
jump
alkali
sample preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602006019203T
Other languages
English (en)
Inventor
Kui Gao
Michael M Becker
Wen Wu
Jeffrey M Linnen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gen Probe Inc
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Publication of DE602006019203D1 publication Critical patent/DE602006019203D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE602006019203T 2005-02-18 2006-02-17 Probenherstellungsverfahren mit einem alkali-stoss Active DE602006019203D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US65419905P 2005-02-18 2005-02-18
US66919205P 2005-04-06 2005-04-06
PCT/US2006/005725 WO2006089154A1 (en) 2005-02-18 2006-02-17 Sample preparation method incorporating an alkaline shock

Publications (1)

Publication Number Publication Date
DE602006019203D1 true DE602006019203D1 (de) 2011-02-10

Family

ID=36603476

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602006019203T Active DE602006019203D1 (de) 2005-02-18 2006-02-17 Probenherstellungsverfahren mit einem alkali-stoss

Country Status (9)

Country Link
US (4) US7510837B2 (de)
EP (2) EP2243832A3 (de)
JP (1) JP4819064B2 (de)
AT (1) ATE493494T1 (de)
AU (1) AU2006214141B2 (de)
CA (1) CA2597319C (de)
DE (1) DE602006019203D1 (de)
DK (1) DK1853706T3 (de)
WO (1) WO2006089154A1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4819064B2 (ja) 2005-02-18 2011-11-16 ジェン−プロウブ インコーポレイテッド アルカリショックを取り入れたサンプル調製法
US20070190526A1 (en) * 2006-02-16 2007-08-16 Nexgen Diagnostics Llc Methods of extracting nucleic acids
US10503872B2 (en) * 2006-09-29 2019-12-10 Gearbox Llc Computational systems for biomedical data
EP3392351A1 (de) * 2008-04-21 2018-10-24 Gen-Probe Incorporated Verfahren zur erkennung des chikungunya-virus
EP2588629B1 (de) * 2010-06-30 2017-05-17 Gen-Probe Incorporated Methode und apparatur zur identifizierung von analyt-beinhaltenden proben unter verwendung von single-read determination des analyten und prozesskontrollsignalen
EP3589408A1 (de) 2017-03-03 2020-01-08 Gen-Probe Incorporated Verdampfungsbegrenzende einsätze für reagenzbehälter und zugehörige verfahren zur verwendung
CA3055989A1 (en) 2017-03-24 2018-09-27 Gen-Probe Incorporated System for mixing contents of containers and related methods of use
EP4286055A3 (de) 2017-07-10 2024-02-28 Gen-Probe Incorporated Analytische systeme und verfahren zur nukleinsäure-amplifikation mit der verwendung von den proben zugeteilten parametern
US20210071242A1 (en) 2018-01-29 2021-03-11 Gen-Probe Incorporated Analytical systems and methods
US20210269854A1 (en) * 2018-06-28 2021-09-02 Gen-Probe Incorporated Sample preparation method and system
US20210317515A1 (en) 2018-07-10 2021-10-14 Gen-Probe Incorporated Methods and systems for detecting and quantifying nucleic acids
US20220396786A1 (en) * 2019-11-14 2022-12-15 Gen-Probe Incorporated Compositions and Methods for Capturing Target Nucleic Acids

Family Cites Families (33)

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US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4868105A (en) 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
DE3785658T2 (de) 1986-08-11 1993-08-12 Siska Diagnostics Inc Methoden und zusammensetzungen fuer tests mit nukleinsaeuresonden.
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
JP2774121B2 (ja) * 1987-07-31 1998-07-09 ザ ボード オブ トラスティーズ オブ ザ リーランド スタンフォード ジュニア ユニバーシティ 標的ポリヌクレオチド配列の選択的増幅
US5283174A (en) * 1987-09-21 1994-02-01 Gen-Probe, Incorporated Homogenous protection assay
US5639604A (en) 1987-09-21 1997-06-17 Gen-Probe Incorporated Homogeneous protection assay
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US4895650A (en) 1988-02-25 1990-01-23 Gen-Probe Incorporated Magnetic separation rack for diagnostic assays
US5130238A (en) * 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
JP3276955B2 (ja) 1988-09-30 2002-04-22 ジーン−トラック・システムス Ｒｎａの鋳型の末端に結合したプローブ構造およびその使用方法
US5656207A (en) * 1989-06-24 1997-08-12 Gen Probe Incorporated Detecting or quantifying multiple analytes using labelling techniques
CA2020958C (en) * 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
ES2214472T3 (es) 1991-08-02 2004-09-16 Biomerieux B.V. Cuantificacion de acidos nucleicos.
DE4212555A1 (de) * 1992-04-15 1993-10-21 Boehringer Mannheim Gmbh Verfahren zum Nachweis von Nukleinsäuren
KR100249110B1 (ko) 1992-05-06 2000-04-01 다니엘 엘. 캐시앙 핵산 서열 증폭 방법, 이에 이용되는 조성물 및 키트
WO1994003472A1 (en) 1992-08-04 1994-02-17 Gen-Probe Incorporated Nucleic acid sequence amplification
US5386024A (en) * 1993-02-10 1995-01-31 Gen-Probe Incorporated Method to prepare nucleic acids from a biological sample using low pH and acid protease
KR100189229B1 (ko) 1993-07-23 1999-06-01 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 핵산 증폭을 강화하는 방법
ATE340866T1 (de) * 1994-10-28 2006-10-15 Gen Probe Inc Zusammensetzungen und verfahren für die gleichzeitige detektion und quantifizierung von einer mehrheit spezifischer nuklein säure sequenzen
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US6534273B2 (en) * 1997-05-02 2003-03-18 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
ATE340868T1 (de) 1997-05-02 2006-10-15 Gen Probe Inc Zwei-schritt hybridisierung und einfang von einem polynukleotid
US6914137B2 (en) * 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
JP3856174B2 (ja) * 1997-12-11 2006-12-13 東洋紡績株式会社 植物ｄｎａの抽出精製方法
PT1206571E (pt) * 1999-05-04 2004-12-31 Ortho Clinical Diagnostics Inc Captura rapida e eficaz de adn a partir duma amostra sem recurso a um reagente de lise celular
US7893228B2 (en) * 2001-10-12 2011-02-22 Qiagen North American Holdings, Inc. Compositions and methods for using a solid support to purify RNA
US6682884B2 (en) * 2002-01-03 2004-01-27 Vijay Sharma Method and device for the rapid clinical diagnosis of hepatitis B virus (HBV) infection in biological samples
ES2370169T3 (es) 2002-06-14 2011-12-13 Gen-Probe Incorporated Composiciones y métodos para detectar el virus de la hepatitis b.
KR20130143677A (ko) * 2004-11-05 2013-12-31 퀴아젠 노쓰 아메리칸 홀딩즈, 인크. 안정화 시약으로부터 핵산을 정제하기 위한 조성물 및 방법
JP4819064B2 (ja) * 2005-02-18 2011-11-16 ジェン−プロウブ インコーポレイテッド アルカリショックを取り入れたサンプル調製法

Also Published As

Publication number Publication date
US8420317B2 (en) 2013-04-16
EP2243832A2 (de) 2010-10-27
EP1853706A1 (de) 2007-11-14
AU2006214141A1 (en) 2006-08-24
DK1853706T3 (da) 2011-03-07
US7803581B2 (en) 2010-09-28
US20120071360A1 (en) 2012-03-22
US20060188912A1 (en) 2006-08-24
ATE493494T1 (de) 2011-01-15
AU2006214141B2 (en) 2010-07-15
CA2597319C (en) 2014-09-30
US8071301B2 (en) 2011-12-06
JP2008529553A (ja) 2008-08-07
WO2006089154A1 (en) 2006-08-24
CA2597319A1 (en) 2006-08-24
US7510837B2 (en) 2009-03-31
US20090191596A1 (en) 2009-07-30
JP4819064B2 (ja) 2011-11-16
US20110008847A1 (en) 2011-01-13
EP1853706B1 (de) 2010-12-29
EP2243832A3 (de) 2011-01-05

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