CY1116526T1 - Διαδικασια για καθαρισμο πρωτεϊνων - Google Patents
Διαδικασια για καθαρισμο πρωτεϊνωνInfo
- Publication number
- CY1116526T1 CY1116526T1 CY20151100535T CY151100535T CY1116526T1 CY 1116526 T1 CY1116526 T1 CY 1116526T1 CY 20151100535 T CY20151100535 T CY 20151100535T CY 151100535 T CY151100535 T CY 151100535T CY 1116526 T1 CY1116526 T1 CY 1116526T1
- Authority
- CY
- Cyprus
- Prior art keywords
- recombinant
- recombinant protein
- host cell
- cleaning procedure
- isomerase
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
- C07K16/241—Tumor Necrosis Factors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
Abstract
Η παρούσα εφεύρεση παρέχει μια μέθοδο για τον καθαρισμό μιας ανασυνδυαστικής πρωτεΐνης από ένα αρνητικό κατά gram βακτηριακό δείγμα κυττάρου ξενιστή ή ένα εκχύλισμα αυτού, όπου το εν λόγω κύτταρο ξενιστή εκφράζει μια ανασυνδυαστική πρωτεΐνη και μια ανασυνδυαστική ισομεράση δισουλφιδίου DsbC. Η εφεύρεση περιλαμβάνει: α. την προσαρμογή του pΗ του δείγματος κυττάρου ξενιστή ή ενός εκχυλίσματος αυτού σε ένα pΗ 5 ή λιγότερο προκειμένου να καθιζάσει την ανασυνδυαστική ισομεράση δισουλφιδίου και β. τον διαχωρισμό της καθιζημένης ανασυνδυαστικής ισομεράσης δισουλφιδίου DsbC από την ανασυνδυαστική πρωτεΐνη ώστε να παράσχει ένα δείγμα ανασυνδυαστικής πρωτεΐνης.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB1012599.5A GB201012599D0 (en) | 2010-07-27 | 2010-07-27 | Process for purifying proteins |
EP11745810.9A EP2598516B8 (en) | 2010-07-27 | 2011-07-27 | Process for purifying proteins |
Publications (1)
Publication Number | Publication Date |
---|---|
CY1116526T1 true CY1116526T1 (el) | 2017-03-15 |
Family
ID=42752868
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CY20151100535T CY1116526T1 (el) | 2010-07-27 | 2015-06-23 | Διαδικασια για καθαρισμο πρωτεϊνων |
Country Status (24)
Country | Link |
---|---|
US (1) | US9751930B2 (el) |
EP (1) | EP2598516B8 (el) |
JP (1) | JP5935222B2 (el) |
KR (1) | KR101944121B1 (el) |
CN (1) | CN103189385B (el) |
AU (1) | AU2011284548B2 (el) |
BR (1) | BR112013000177B1 (el) |
CA (1) | CA2804099C (el) |
CY (1) | CY1116526T1 (el) |
DK (1) | DK2598516T3 (el) |
ES (1) | ES2537877T3 (el) |
GB (1) | GB201012599D0 (el) |
HR (1) | HRP20150639T1 (el) |
HU (1) | HUE026049T2 (el) |
IL (1) | IL224044A (el) |
ME (1) | ME02162B (el) |
MX (1) | MX337184B (el) |
PL (1) | PL2598516T3 (el) |
PT (1) | PT2598516E (el) |
RS (1) | RS54085B1 (el) |
SG (2) | SG196862A1 (el) |
SI (1) | SI2598516T1 (el) |
SM (1) | SMT201500142B (el) |
WO (1) | WO2012013930A2 (el) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
PL2993231T3 (pl) | 2009-09-24 | 2019-01-31 | Ucb Biopharma Sprl | Szczep bakteryjny do ekspresji rekombinowanego białka mający degp pozbawione aktywności proteazowej przy zachowaniu aktywności białka opiekuńczego i nokauty genów tsp i ptr |
GB201000590D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial host strain |
GB201000587D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial hoist strain |
GB201000591D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial hoist strain |
GB201001791D0 (en) | 2010-02-03 | 2010-03-24 | Ucb Pharma Sa | Process for obtaining antibodies |
PT2731973T (pt) | 2011-07-13 | 2018-02-22 | Ucb Biopharma Sprl | Estirpe hospedeira bacteriana que expressa dsbc recombinante |
GB201208367D0 (en) * | 2012-05-14 | 2012-06-27 | Ucb Pharma Sa | Biological product |
WO2013180648A1 (en) * | 2012-05-31 | 2013-12-05 | Agency For Science, Technology And Research | Methods for use of mixed multifunctional surfaces for reducing aggregate content in protein preparations |
EP2855504B1 (en) | 2012-05-31 | 2018-10-24 | Agency For Science, Technology And Research | Chromatographic purification of immunoglobulin g preparations with particles having multimodal functionalities |
AU2015371341B2 (en) | 2014-12-22 | 2021-05-20 | Ucb Biopharma Sprl | Method of protein manufacture |
CA2969889A1 (en) * | 2014-12-22 | 2016-06-30 | Ucb Biopharma Sprl | Protein manufacture |
SI3265557T1 (sl) * | 2015-03-06 | 2020-02-28 | F. Hoffmann-La Roche Ag | Ultraprečiščena DsbA in DsbC ter postopki njune izdelave in uporabe |
CN110272491B (zh) * | 2018-03-13 | 2023-01-24 | 江苏恒瑞医药股份有限公司 | 一种抗pd-1抗体的纯化工艺 |
KR20220031670A (ko) * | 2019-08-05 | 2022-03-11 | 와커 헤미 아게 | 발효 방법에서 재조합 단백질 방출용 박테리아 균주 |
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WO1993008829A1 (en) | 1991-11-04 | 1993-05-13 | The Regents Of The University Of California | Compositions that mediate killing of hiv-infected cells |
GB9215540D0 (en) | 1992-07-22 | 1992-09-02 | Celltech Ltd | Protein expression system |
US6027888A (en) * | 1996-04-05 | 2000-02-22 | Board Of Regents, The University Of Texas System | Methods for producing soluble, biologically-active disulfide-bond containing eukaryotic proteins in bacterial cells |
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US6083715A (en) * | 1997-06-09 | 2000-07-04 | Board Of Regents, The University Of Texas System | Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells |
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AU8867501A (en) | 2000-09-01 | 2002-03-13 | Biogen Inc | Co-crystal structure of monoclonal antibody 5c8 and cd154, and use thereof in drug design |
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US7041479B2 (en) * | 2000-09-06 | 2006-05-09 | The Board Of Trustess Of The Leland Stanford Junior University | Enhanced in vitro synthesis of active proteins containing disulfide bonds |
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PL2993231T3 (pl) | 2009-09-24 | 2019-01-31 | Ucb Biopharma Sprl | Szczep bakteryjny do ekspresji rekombinowanego białka mający degp pozbawione aktywności proteazowej przy zachowaniu aktywności białka opiekuńczego i nokauty genów tsp i ptr |
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GB201000588D0 (en) * | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial host strain |
GB201000591D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial hoist strain |
GB201000587D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial hoist strain |
GB201000590D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial host strain |
GB201001791D0 (en) | 2010-02-03 | 2010-03-24 | Ucb Pharma Sa | Process for obtaining antibodies |
PT2731973T (pt) | 2011-07-13 | 2018-02-22 | Ucb Biopharma Sprl | Estirpe hospedeira bacteriana que expressa dsbc recombinante |
EP2546267A1 (en) | 2011-07-13 | 2013-01-16 | UCB Pharma S.A. | Bacterial host strain expressing recombinant DsbC |
GB201208367D0 (en) | 2012-05-14 | 2012-06-27 | Ucb Pharma Sa | Biological product |
-
2010
- 2010-07-27 GB GBGB1012599.5A patent/GB201012599D0/en not_active Ceased
-
2011
- 2011-07-27 EP EP11745810.9A patent/EP2598516B8/en active Active
- 2011-07-27 AU AU2011284548A patent/AU2011284548B2/en active Active
- 2011-07-27 US US13/812,350 patent/US9751930B2/en active Active
- 2011-07-27 PT PT117458109T patent/PT2598516E/pt unknown
- 2011-07-27 ES ES11745810.9T patent/ES2537877T3/es active Active
- 2011-07-27 PL PL11745810T patent/PL2598516T3/pl unknown
- 2011-07-27 WO PCT/GB2011/001129 patent/WO2012013930A2/en active Application Filing
- 2011-07-27 CN CN201180036417.8A patent/CN103189385B/zh active Active
- 2011-07-27 SG SG2014005441A patent/SG196862A1/en unknown
- 2011-07-27 HU HUE11745810A patent/HUE026049T2/en unknown
- 2011-07-27 MX MX2013000899A patent/MX337184B/es active IP Right Grant
- 2011-07-27 RS RS20150429A patent/RS54085B1/en unknown
- 2011-07-27 SI SI201130516T patent/SI2598516T1/sl unknown
- 2011-07-27 JP JP2013521208A patent/JP5935222B2/ja active Active
- 2011-07-27 KR KR1020137004911A patent/KR101944121B1/ko active IP Right Grant
- 2011-07-27 BR BR112013000177-1A patent/BR112013000177B1/pt not_active IP Right Cessation
- 2011-07-27 SG SG2013001318A patent/SG186974A1/en unknown
- 2011-07-27 DK DK11745810.9T patent/DK2598516T3/da active
- 2011-07-27 ME MEP-2015-94A patent/ME02162B/me unknown
- 2011-07-27 CA CA2804099A patent/CA2804099C/en active Active
-
2012
- 2012-12-31 IL IL224044A patent/IL224044A/en active IP Right Grant
-
2015
- 2015-06-15 HR HRP20150639TT patent/HRP20150639T1/hr unknown
- 2015-06-18 SM SM201500142T patent/SMT201500142B/xx unknown
- 2015-06-23 CY CY20151100535T patent/CY1116526T1/el unknown
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