CY1112394T1 - Παραγωγη fgf18 σε προκαρυωτικους ξενιστες - Google Patents

Παραγωγη fgf18 σε προκαρυωτικους ξενιστες

Info

Publication number
CY1112394T1
CY1112394T1 CY20111101149T CY111101149T CY1112394T1 CY 1112394 T1 CY1112394 T1 CY 1112394T1 CY 20111101149 T CY20111101149 T CY 20111101149T CY 111101149 T CY111101149 T CY 111101149T CY 1112394 T1 CY1112394 T1 CY 1112394T1
Authority
CY
Cyprus
Prior art keywords
fgf18
coli
students
preventive
production
Prior art date
Application number
CY20111101149T
Other languages
English (en)
Inventor
Brian J Reardon
Susan D Julien
Chung-Leung Chan
Hong Y Liu
Original Assignee
Zymogenetics, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zymogenetics, Inc. filed Critical Zymogenetics, Inc.
Publication of CY1112394T1 publication Critical patent/CY1112394T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/50—Fibroblast growth factor [FGF]
    • C07K14/501—Fibroblast growth factor [FGF] acidic FGF [aFGF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/50—Fibroblast growth factor [FGF]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Περιγράφονται πλασμίδια έκφρασης και μέθοδοι που χρησιμοποιούν σύστημα έκφρασης E. coli για την υψηλής κλίμακας παραγωγή FGF18. Τα πλασμίδια χρησιμοποιούν την κωδικοποιητική αλληλουχία της FGF18 με συγκεκριμένες αλλαγές στα νουκλεοτίδια ώστε να βελτιστοποιηθούν τα κωδικόνια και η δευτεροταγής δομή του mRNA για μετάφραση στο E. coli. Χρησιμοποιώντας τα πλασμίδια έκφρασης, το γονίδιο FGF18 παρήχθη στο E. coli σε επίπεδο μεγαλύτερο του 1 g/L σε αντιδραστήρα διαλείποντος έργου. Περιλαμβάνονται επίσης στελέχη E. coli με ανεπάρκεια στην OmpT, καθώς και στελέχη αρνητικά σε OmpT και fhuA μετασχηματισμένα με πλασμίδια έκφρασης FGF18.
CY20111101149T 2004-12-10 2011-11-24 Παραγωγη fgf18 σε προκαρυωτικους ξενιστες CY1112394T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US63502304P 2004-12-10 2004-12-10
EP05853969A EP1828239B1 (en) 2004-12-10 2005-12-12 Fgf18 production in prokaryotic hosts

Publications (1)

Publication Number Publication Date
CY1112394T1 true CY1112394T1 (el) 2015-12-09

Family

ID=36061577

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20111101149T CY1112394T1 (el) 2004-12-10 2011-11-24 Παραγωγη fgf18 σε προκαρυωτικους ξενιστες

Country Status (13)

Country Link
US (1) US7858341B2 (el)
EP (1) EP1828239B1 (el)
AT (1) ATE521633T1 (el)
AU (1) AU2005314438B2 (el)
CA (1) CA2592261C (el)
CY (1) CY1112394T1 (el)
DK (1) DK1828239T3 (el)
ES (1) ES2371219T3 (el)
HK (1) HK1113161A1 (el)
PL (1) PL1828239T3 (el)
PT (1) PT1828239E (el)
SI (1) SI1828239T1 (el)
WO (1) WO2006063362A1 (el)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1931704T3 (da) * 2005-10-04 2011-03-14 Zymogenetics L L C Frembringelse og rensning af IL-29
SI2054050T1 (sl) * 2006-08-25 2012-12-31 Ares Trading S.A. Zdravljenje hrustanäśnih obolenj z fgf-18
WO2008023063A2 (en) 2006-08-25 2008-02-28 Ares Trading S.A. Treatment of cartilage disorders with fgf-18
MX2011013417A (es) 2009-06-25 2012-03-29 Amgen Inc Procesos de purificacion por captura para proteinas expresadas en un sistema no mamifero.
WO2012038953A2 (en) 2010-09-21 2012-03-29 Hepacore Ltd. Fgf-18 truncated variants having increased receptor specificity and uses thereof
WO2012140650A2 (en) 2011-04-12 2012-10-18 Hepacore Ltd. Conjugates of carboxy polysaccharides with fibroblast growth factors and variants thereof
TW201307563A (zh) * 2011-05-19 2013-02-16 Shire Human Genetic Therapies 純化乙醯肝素－ｎ－硫酸酯酶之方法
TWI527590B (zh) 2011-06-17 2016-04-01 艾瑞斯貿易公司 Ｆｇｆ－１８之凍乾調配物
US9580692B2 (en) * 2012-02-29 2017-02-28 Ge Healthcare Bioprocess R&D Ab Method for endotoxin removal
US10041050B2 (en) 2012-02-29 2018-08-07 Ge Healthcare Bioprocess R&D Ab Method for endotoxin removal
CN104736723B (zh) 2012-08-06 2017-10-31 默克专利有限公司 用于预测对fgf‑18化合物的响应性的遗传标记
CA2938796C (en) * 2014-02-20 2022-08-02 Merck Patent Gmbh Implant comprising fgf-18
KR101608734B1 (ko) 2014-03-21 2016-04-04 씨제이제일제당 주식회사 L-아미노산을 생산하는 미생물 및 이를 이용하여 l-아미노산을 생산하는 방법
CA2971315C (en) 2015-01-29 2023-02-14 Ares Trading S.A. Immunoassays for high positively charged proteins
EP3957654A1 (en) * 2015-07-15 2022-02-23 Prosit Sole Biotechnology (Beijing) Co., Ltd Fusion polypeptides and methods of use
ES2918249T3 (es) 2017-09-29 2022-07-14 Merck Patent Gmbh Biomarcadores inflamatorios para predecir la capacidad de respuesta al compuesto FGF-18
PL3687558T3 (pl) 2017-09-29 2022-07-18 Merck Patent Gmbh Biomarkery metaboliczne do przewidywania podatności na leczenie związkiem fgf-18
CN110759986B (zh) * 2019-10-18 2023-01-31 大连工业大学 一种可逆自组装蛋白的高效制备方法
CN114181321B (zh) * 2021-12-10 2023-04-11 中国海洋大学 花鲈fgf6a、fgf6b以及fgf18重组蛋白及其制备方法和应用
WO2024084807A1 (ja) * 2022-10-19 2024-04-25 学校法人東邦大学 線維芽細胞増殖因子１８の測定方法及び測定用試薬

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5439818A (en) 1985-09-12 1995-08-08 Scios Nova Inc. DNA encoding human recombinant basic fibroblast growth factor
US5604293A (en) 1985-09-12 1997-02-18 Scios Inc. Recombinant human basic fibroblast growth factor
US5773252A (en) 1995-06-05 1998-06-30 Human Genome Sciences, Inc. Fibroblast growth factor 15
US20050043234A1 (en) 1996-10-16 2005-02-24 Deisher Theresa A. Novel FGF homologs
IL129379A (en) 1996-10-16 2008-12-29 Zymogenetics Inc Fibroblast growth factor homologues
US7135459B2 (en) 1996-10-16 2006-11-14 Zymogenetics, Inc. Methods of use of FGF homologs
US6518236B1 (en) 1996-10-16 2003-02-11 Zymogenetics, Inc. FGF homologs
WO2001039788A2 (en) 1999-12-02 2001-06-07 Zymogenetics, Inc. Methods for targeting cells that express fibroblast growth receptor-3 or-2
AU2003279835B2 (en) 2002-10-07 2009-09-10 Zymogenetics, Inc. Methods of administering FGF18
US20040229292A1 (en) 2002-11-26 2004-11-18 Sebastiano Cavallaro Use of FGF-18 in the diagnosis and treatment of memory disorders

Also Published As

Publication number Publication date
HK1113161A1 (en) 2008-09-26
WO2006063362A1 (en) 2006-06-15
AU2005314438A1 (en) 2006-06-15
PL1828239T3 (pl) 2012-01-31
AU2005314438B2 (en) 2012-03-29
PT1828239E (pt) 2011-10-27
US20060172384A1 (en) 2006-08-03
US7858341B2 (en) 2010-12-28
CA2592261C (en) 2015-11-24
EP1828239A1 (en) 2007-09-05
EP1828239B1 (en) 2011-08-24
DK1828239T3 (da) 2011-12-19
CA2592261A1 (en) 2006-06-15
SI1828239T1 (sl) 2011-11-30
ATE521633T1 (de) 2011-09-15
ES2371219T3 (es) 2011-12-28

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