CY1107118T1 - Βελτιωμενη μεθοδος κατεργασιας με οξινο θειωδες αλας - Google Patents

Βελτιωμενη μεθοδος κατεργασιας με οξινο θειωδες αλας

Info

Publication number
CY1107118T1
CY1107118T1 CY20081100002T CY081100002T CY1107118T1 CY 1107118 T1 CY1107118 T1 CY 1107118T1 CY 20081100002 T CY20081100002 T CY 20081100002T CY 081100002 T CY081100002 T CY 081100002T CY 1107118 T1 CY1107118 T1 CY 1107118T1
Authority
CY
Cyprus
Prior art keywords
solid phase
glass
nucleic acid
treatment
improved method
Prior art date
Application number
CY20081100002T
Other languages
English (en)
Inventor
Christine Dr Markert-Hahn
Dirk Dr Block
Original Assignee
F. Hoffmann-La Roche Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=32071078&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CY1107118(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from EP02019097A external-priority patent/EP1394172A1/en
Application filed by F. Hoffmann-La Roche Ag filed Critical F. Hoffmann-La Roche Ag
Publication of CY1107118T1 publication Critical patent/CY1107118T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Compounds Of Iron (AREA)
  • Saccharide Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

Η παρούσα δήλωση αναφέρεται σε μια μέθοδο διεξαγωγής μιας αντίδρασης όξινου θειώδους άλατος για τον καθορισμό των θέσεων μεθυλίωσης σε ένα νουκλεϊκό οξύ, ήτοι μεθυλιωμένες και μη μεθυλιωμένες κυτοσίνες, κατά την οποία το νουκλεϊκό οξύ είναι συνδεδεμένο με μια στερεά φάση κατά τη διάρκεια της βαθμίδας απαμίνωσης ή/και αποσουλφόνωσης της αντίδρασης όξινου θειώδους άλατος. Η στερεά φάση είναι κατά προτίμηση ένα υλικό που περιέχει ύαλο ή πυριτία, προτιμότερα μαλλί υάλου, υάλινη μεμβράνη ή μαγνητικό υάλινο σωμάτιο. Περαιτέρω, αποκαλύπτεται η χρήση μιας στερεάς φάσης για σύνδεση ενός νουκλεϊκού οξέος κατά τη διάρκεια της βαθμίδας απαμίνωσης ή/και αποσουλφόνωσης της αντίδρασης όξινου θειώδους άλατος και ένα κυτίο συνέργων (κιτ) που περιέχει ένα αντιδραστήριο όξινου θειώδους άλατος και μια στερεά φάση.
CY20081100002T 2002-08-29 2008-01-02 Βελτιωμενη μεθοδος κατεργασιας με οξινο θειωδες αλας CY1107118T1 (el)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP02019097A EP1394172A1 (en) 2002-08-29 2002-08-29 Improved method for bisulfite treatment
EP02028114 2002-12-18
EP03019321A EP1394173B9 (en) 2002-08-29 2003-08-27 Improved method for bisulfite treatment

Publications (1)

Publication Number Publication Date
CY1107118T1 true CY1107118T1 (el) 2012-10-24

Family

ID=32071078

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20081100002T CY1107118T1 (el) 2002-08-29 2008-01-02 Βελτιωμενη μεθοδος κατεργασιας με οξινο θειωδες αλας

Country Status (15)

Country Link
US (2) US9394332B2 (el)
EP (2) EP1829886B1 (el)
JP (2) JP5153048B2 (el)
CN (1) CN1271081C (el)
AT (1) ATE374782T1 (el)
AU (1) AU2003236461B2 (el)
CA (1) CA2438327C (el)
CY (1) CY1107118T1 (el)
DE (1) DE60316642T2 (el)
DK (2) DK1829886T3 (el)
ES (2) ES2545577T3 (el)
HK (1) HK1062688A1 (el)
HU (1) HUE027240T2 (el)
PT (1) PT1394173E (el)
SI (2) SI1829886T1 (el)

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Also Published As

Publication number Publication date
US9868756B2 (en) 2018-01-16
EP1829886B1 (en) 2015-07-29
CA2438327A1 (en) 2004-02-29
HUE027240T2 (en) 2016-10-28
SI1394173T1 (sl) 2008-02-29
DK1829886T3 (en) 2015-08-17
JP5153048B2 (ja) 2013-02-27
EP1394173A1 (en) 2004-03-03
EP1829886A3 (en) 2008-10-15
AU2003236461A1 (en) 2004-03-18
ES2545577T3 (es) 2015-09-14
CN1271081C (zh) 2006-08-23
JP5310059B2 (ja) 2013-10-09
JP2004089195A (ja) 2004-03-25
DK1394173T3 (da) 2008-02-04
PT1394173E (pt) 2007-11-28
JP2009106304A (ja) 2009-05-21
CN1495192A (zh) 2004-05-12
US20160068888A1 (en) 2016-03-10
CA2438327C (en) 2008-04-15
EP1394173B1 (en) 2007-10-03
EP1829886A2 (en) 2007-09-05
DE60316642D1 (de) 2007-11-15
EP1394173B9 (en) 2008-10-15
AU2003236461B2 (en) 2009-05-28
US9394332B2 (en) 2016-07-19
ATE374782T1 (de) 2007-10-15
HK1062688A1 (en) 2004-11-19
SI1829886T1 (sl) 2015-12-31
ES2294231T3 (es) 2008-04-01
US20040241704A1 (en) 2004-12-02
DE60316642T2 (de) 2008-01-31

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