CO2022011682A2 - Método para producir linfocitos citolíticos naturales a partir de células madre pluripotentes - Google Patents
Método para producir linfocitos citolíticos naturales a partir de células madre pluripotentesInfo
- Publication number
- CO2022011682A2 CO2022011682A2 CONC2022/0011682A CO2022011682A CO2022011682A2 CO 2022011682 A2 CO2022011682 A2 CO 2022011682A2 CO 2022011682 A CO2022011682 A CO 2022011682A CO 2022011682 A2 CO2022011682 A2 CO 2022011682A2
- Authority
- CO
- Colombia
- Prior art keywords
- cells
- pluripotent stem
- natural killer
- producing natural
- stem cells
- Prior art date
Links
- 210000000822 natural killer cell Anatomy 0.000 title abstract 2
- 210000001778 pluripotent stem cell Anatomy 0.000 title abstract 2
- 238000004519 manufacturing process Methods 0.000 title 1
- 238000000034 method Methods 0.000 abstract 2
- 101000581981 Homo sapiens Neural cell adhesion molecule 1 Proteins 0.000 abstract 1
- 102100027347 Neural cell adhesion molecule 1 Human genes 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 210000002865 immune cell Anatomy 0.000 abstract 1
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0646—Natural killers cells [NK], NKT cells
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- A61K35/14—Blood; Artificial blood
- A61K35/17—Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes
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Abstract
La presente descripción proporciona, entre otras cosas, un método para producir eficazmente linfocitos citolíticos naturales a partir de células pluripotentes inducidas. El método incluye los pasos de: (I) cultivar células madre pluripotentes en un medio de cultivo para producir células inmunitarias CD56+/CD3-.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US202062983511P | 2020-02-28 | 2020-02-28 | |
PCT/US2021/019917 WO2021174004A1 (en) | 2020-02-28 | 2021-02-26 | Method for producing natural killer cells from pluripotent stem cells |
Publications (1)
Publication Number | Publication Date |
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CO2022011682A2 true CO2022011682A2 (es) | 2022-08-30 |
Family
ID=75108897
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CONC2022/0011682A CO2022011682A2 (es) | 2020-02-28 | 2022-08-19 | Método para producir linfocitos citolíticos naturales a partir de células madre pluripotentes |
Country Status (14)
Country | Link |
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US (1) | US20210292713A1 (es) |
EP (1) | EP4110904A1 (es) |
JP (1) | JP2023516632A (es) |
KR (1) | KR20220148174A (es) |
CN (1) | CN115427555A (es) |
AR (1) | AR121455A1 (es) |
AU (1) | AU2021225952A1 (es) |
BR (1) | BR112022017216A2 (es) |
CA (1) | CA3169617A1 (es) |
CO (1) | CO2022011682A2 (es) |
IL (1) | IL295964A (es) |
MX (1) | MX2022010521A (es) |
TW (1) | TW202146646A (es) |
WO (1) | WO2021174004A1 (es) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2022144632A1 (en) | 2020-12-30 | 2022-07-07 | Crispr Therapeutics Ag | Compositions and methods for differentiating stem cells into nk cells |
CN113801846A (zh) * | 2021-09-22 | 2021-12-17 | 南京艾尔普再生医学科技有限公司 | 一种从人诱导多能干细胞分化为自然杀伤细胞的方法 |
CN114517176B (zh) * | 2022-01-27 | 2022-12-02 | 广东普罗凯融生物医药科技有限公司 | 一种将ips细胞诱导成nk细胞的试剂盒及其应用方法 |
WO2024101862A1 (ko) * | 2022-11-07 | 2024-05-16 | 마루테라퓨틱스 주식회사 | Nk 세포의 분화 효율 및 기능성 증가를 위한 배양 방법 |
CN115537397B (zh) * | 2022-12-02 | 2023-03-24 | 广东先康达细胞库有限公司 | Nk细胞诱导培养基及其培养方法 |
CN116410336B (zh) * | 2023-06-02 | 2023-09-22 | 云南赛元生物技术有限公司 | 一种嵌合抗原受体的编码核苷酸、car-nk细胞及其构建方法和应用 |
CN117050941B (zh) * | 2023-10-11 | 2024-01-26 | 苏州艾凯利元生物科技有限公司 | 一种制备自然杀伤细胞的方法 |
CN117050940B (zh) * | 2023-10-11 | 2024-01-26 | 苏州艾凯利元生物科技有限公司 | 一种制备自然杀伤细胞的方法 |
Family Cites Families (40)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4797368A (en) | 1985-03-15 | 1989-01-10 | The United States Of America As Represented By The Department Of Health And Human Services | Adeno-associated virus as eukaryotic expression vector |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US5139941A (en) | 1985-10-31 | 1992-08-18 | University Of Florida Research Foundation, Inc. | AAV transduction vectors |
US6013516A (en) | 1995-10-06 | 2000-01-11 | The Salk Institute For Biological Studies | Vector and method of use for nucleic acid delivery to non-dividing cells |
US5928906A (en) | 1996-05-09 | 1999-07-27 | Sequenom, Inc. | Process for direct sequencing during template amplification |
US5994136A (en) | 1997-12-12 | 1999-11-30 | Cell Genesys, Inc. | Method and means for producing high titer, safe, recombinant lentivirus vectors |
US9453219B2 (en) | 2003-05-15 | 2016-09-27 | Mello Biotech Taiwan Co., Ltd. | Cosmetic designs and products using intronic RNA |
CN101864392B (zh) | 2005-12-13 | 2016-03-23 | 国立大学法人京都大学 | 核重新编程因子 |
JP5813321B2 (ja) | 2007-03-23 | 2015-11-17 | ウィスコンシン アラムニ リサーチ ファンデーション | 体細胞の再プログラム化 |
JP2008307007A (ja) | 2007-06-15 | 2008-12-25 | Bayer Schering Pharma Ag | 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞 |
CN101855338B (zh) | 2007-08-31 | 2013-07-17 | 怀特黑德生物医学研究所 | 在程序重排体细胞中的wnt途径刺激 |
WO2009057831A1 (ja) | 2007-10-31 | 2009-05-07 | Kyoto University | 核初期化方法 |
WO2009075119A1 (ja) | 2007-12-10 | 2009-06-18 | Kyoto University | 効率的な核初期化方法 |
WO2009079007A1 (en) | 2007-12-17 | 2009-06-25 | Gliamed, Inc. | Stem-like cells and method for reprogramming adult mammalian somatic cells |
CN101970664B (zh) | 2008-01-16 | 2013-08-21 | 林希龙 | 使用可诱导的重组核糖核酸因子生成不含肿瘤的类胚胎干细胞的多能性细胞 |
EP2250252A2 (en) | 2008-02-11 | 2010-11-17 | Cambridge Enterprise Limited | Improved reprogramming of mammalian cells, and the cells obtained |
EP2090649A1 (en) | 2008-02-13 | 2009-08-19 | Fondazione Telethon | Method for reprogramming differentiated cells |
US20110014164A1 (en) | 2008-02-15 | 2011-01-20 | President And Fellows Of Harvard College | Efficient induction of pluripotent stem cells using small molecule compounds |
SG188904A1 (en) | 2008-03-17 | 2013-04-30 | Scripps Research Inst | Combined chemical and genetic approaches for generation of induced pluripotent stem cells |
WO2009114949A1 (en) | 2008-03-20 | 2009-09-24 | UNIVERSITé LAVAL | Methods for deprogramming somatic cells and uses thereof |
AU2009234424A1 (en) | 2008-04-07 | 2009-10-15 | Nupotential, Inc. | Reprogramming a cell by inducing a pluripotent gene through use of an HDAC modulator |
CN101835890B (zh) | 2008-06-27 | 2013-07-24 | 国立大学法人京都大学 | 有效建立诱导的多能干细胞的方法 |
CA2730546A1 (en) | 2008-07-14 | 2010-01-21 | Oklahoma Medical Research Foundation | Production of pluripotent cells through inhibition of bright/arid3a function |
WO2010147612A1 (en) | 2009-06-18 | 2010-12-23 | Lixte Biotechnology, Inc. | Methods of modulating cell regulation by inhibiting p53 |
WO2010033906A2 (en) | 2008-09-19 | 2010-03-25 | President And Fellows Of Harvard College | Efficient induction of pluripotent stem cells using small molecule compounds |
WO2010033920A2 (en) | 2008-09-19 | 2010-03-25 | Whitehead Institute For Biomedical Research | Compositions and methods for enhancing cell reprogramming |
WO2010042800A1 (en) | 2008-10-10 | 2010-04-15 | Nevada Cancer Institute | Methods of reprogramming somatic cells and methods of use for such cells |
JP2012507258A (ja) | 2008-10-30 | 2012-03-29 | 国立大学法人京都大学 | 人工多能性幹細胞の作製方法 |
US20110059526A1 (en) | 2008-11-12 | 2011-03-10 | Nupotential, Inc. | Reprogramming a cell by inducing a pluripotent gene through use of an hdac modulator |
WO2010068955A2 (en) | 2008-12-13 | 2010-06-17 | Dna Microarray | MICROENVIRONMENT NICHE ASSAY FOR CiPS SCREENING |
WO2010098419A1 (en) | 2009-02-27 | 2010-09-02 | Kyoto University | Novel nuclear reprogramming substance |
WO2010102267A2 (en) | 2009-03-06 | 2010-09-10 | Ipierian, Inc. | Tgf-beta pathway inhibitors for enhancement of cellular reprogramming of human cells |
US9340775B2 (en) | 2009-03-25 | 2016-05-17 | The Salk Institute For Biological Studies | Induced pluripotent stem cell produced by transfecting a human neural stem cell with an episomal vector encoding the Oct4 and Nanog proteins |
WO2010111422A2 (en) | 2009-03-25 | 2010-09-30 | The Salk Institute For Biological Studies | Induced pluripotent stem cell generation using two factors and p53 inactivation |
WO2010115050A2 (en) | 2009-04-01 | 2010-10-07 | The Regents Of The University Of California | Embryonic stem cell specific micrornas promote induced pluripotency |
WO2010124290A2 (en) | 2009-04-24 | 2010-10-28 | Whitehead Institute For Biomedical Research | Compositions and methods for deriving or culturing pluripotent cells |
WO2010147395A2 (en) | 2009-06-16 | 2010-12-23 | Korea Research Institute Of Bioscience And Biotechnology | Medium composition comprising neuropeptide y for the generation, maintenance, prologned undifferentiated growth of pluripotent stem cells and method of culturing pluripotent stem cell using the same |
TWI654206B (zh) | 2013-03-16 | 2019-03-21 | 諾華公司 | 使用人類化抗-cd19嵌合抗原受體治療癌症 |
DK3365435T3 (da) * | 2015-10-20 | 2021-04-06 | Fujifilm Cellular Dynamics Inc | Produktion af hæmatopoietiske prækursorceller med multi-afstamning med genetisk programmering |
CN115927199A (zh) * | 2015-11-04 | 2023-04-07 | 菲特治疗公司 | 用于诱导造血细胞分化的方法和组合物 |
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AU2021225952A1 (en) | 2022-10-20 |
EP4110904A1 (en) | 2023-01-04 |
IL295964A (en) | 2022-10-01 |
BR112022017216A2 (pt) | 2022-10-11 |
KR20220148174A (ko) | 2022-11-04 |
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